Job ID = 14168772 SRX = SRX9910771 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 74805909 spots for SRR13498842/SRR13498842.sra Written 74805909 spots for SRR13498842/SRR13498842.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169860 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:49 74805909 reads; of these: 74805909 (100.00%) were unpaired; of these: 6103425 (8.16%) aligned 0 times 45482556 (60.80%) aligned exactly 1 time 23219928 (31.04%) aligned >1 times 91.84% overall alignment rate Time searching: 00:31:49 Overall time: 00:31:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 14549827 / 68702484 = 0.2118 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:55:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:55:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:55:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:55:42: 1000000 INFO @ Fri, 10 Dec 2021 20:55:48: 2000000 INFO @ Fri, 10 Dec 2021 20:55:54: 3000000 INFO @ Fri, 10 Dec 2021 20:56:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:56:06: 5000000 INFO @ Fri, 10 Dec 2021 20:56:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:56:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:56:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:56:13: 6000000 INFO @ Fri, 10 Dec 2021 20:56:13: 1000000 INFO @ Fri, 10 Dec 2021 20:56:19: 7000000 INFO @ Fri, 10 Dec 2021 20:56:19: 2000000 INFO @ Fri, 10 Dec 2021 20:56:26: 8000000 INFO @ Fri, 10 Dec 2021 20:56:26: 3000000 INFO @ Fri, 10 Dec 2021 20:56:32: 9000000 INFO @ Fri, 10 Dec 2021 20:56:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:56:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:56:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:56:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:56:39: 10000000 INFO @ Fri, 10 Dec 2021 20:56:40: 5000000 INFO @ Fri, 10 Dec 2021 20:56:44: 1000000 INFO @ Fri, 10 Dec 2021 20:56:47: 11000000 INFO @ Fri, 10 Dec 2021 20:56:47: 6000000 INFO @ Fri, 10 Dec 2021 20:56:52: 2000000 INFO @ Fri, 10 Dec 2021 20:56:54: 12000000 INFO @ Fri, 10 Dec 2021 20:56:55: 7000000 INFO @ Fri, 10 Dec 2021 20:57:00: 3000000 INFO @ Fri, 10 Dec 2021 20:57:02: 13000000 INFO @ Fri, 10 Dec 2021 20:57:02: 8000000 INFO @ Fri, 10 Dec 2021 20:57:08: 4000000 INFO @ Fri, 10 Dec 2021 20:57:09: 14000000 INFO @ Fri, 10 Dec 2021 20:57:10: 9000000 INFO @ Fri, 10 Dec 2021 20:57:16: 5000000 INFO @ Fri, 10 Dec 2021 20:57:17: 15000000 INFO @ Fri, 10 Dec 2021 20:57:17: 10000000 INFO @ Fri, 10 Dec 2021 20:57:24: 6000000 INFO @ Fri, 10 Dec 2021 20:57:25: 16000000 INFO @ Fri, 10 Dec 2021 20:57:25: 11000000 INFO @ Fri, 10 Dec 2021 20:57:32: 17000000 INFO @ Fri, 10 Dec 2021 20:57:32: 7000000 INFO @ Fri, 10 Dec 2021 20:57:32: 12000000 INFO @ Fri, 10 Dec 2021 20:57:40: 18000000 INFO @ Fri, 10 Dec 2021 20:57:40: 13000000 INFO @ Fri, 10 Dec 2021 20:57:40: 8000000 INFO @ Fri, 10 Dec 2021 20:57:47: 19000000 INFO @ Fri, 10 Dec 2021 20:57:47: 14000000 INFO @ Fri, 10 Dec 2021 20:57:48: 9000000 INFO @ Fri, 10 Dec 2021 20:57:55: 20000000 INFO @ Fri, 10 Dec 2021 20:57:55: 15000000 INFO @ Fri, 10 Dec 2021 20:57:56: 10000000 INFO @ Fri, 10 Dec 2021 20:58:02: 21000000 INFO @ Fri, 10 Dec 2021 20:58:03: 16000000 INFO @ Fri, 10 Dec 2021 20:58:04: 11000000 INFO @ Fri, 10 Dec 2021 20:58:10: 22000000 INFO @ Fri, 10 Dec 2021 20:58:10: 17000000 INFO @ Fri, 10 Dec 2021 20:58:12: 12000000 INFO @ Fri, 10 Dec 2021 20:58:17: 23000000 INFO @ Fri, 10 Dec 2021 20:58:18: 18000000 INFO @ Fri, 10 Dec 2021 20:58:20: 13000000 INFO @ Fri, 10 Dec 2021 20:58:25: 24000000 INFO @ Fri, 10 Dec 2021 20:58:25: 19000000 INFO @ Fri, 10 Dec 2021 20:58:28: 14000000 INFO @ Fri, 10 Dec 2021 20:58:32: 25000000 INFO @ Fri, 10 Dec 2021 20:58:33: 20000000 INFO @ Fri, 10 Dec 2021 20:58:36: 15000000 INFO @ Fri, 10 Dec 2021 20:58:40: 26000000 INFO @ Fri, 10 Dec 2021 20:58:40: 21000000 INFO @ Fri, 10 Dec 2021 20:58:44: 16000000 INFO @ Fri, 10 Dec 2021 20:58:48: 27000000 INFO @ Fri, 10 Dec 2021 20:58:48: 22000000 INFO @ Fri, 10 Dec 2021 20:58:52: 17000000 INFO @ Fri, 10 Dec 2021 20:58:55: 28000000 INFO @ Fri, 10 Dec 2021 20:58:55: 23000000 INFO @ Fri, 10 Dec 2021 20:59:00: 18000000 INFO @ Fri, 10 Dec 2021 20:59:03: 29000000 INFO @ Fri, 10 Dec 2021 20:59:03: 24000000 INFO @ Fri, 10 Dec 2021 20:59:09: 19000000 INFO @ Fri, 10 Dec 2021 20:59:10: 30000000 INFO @ Fri, 10 Dec 2021 20:59:10: 25000000 INFO @ Fri, 10 Dec 2021 20:59:17: 20000000 INFO @ Fri, 10 Dec 2021 20:59:17: 31000000 INFO @ Fri, 10 Dec 2021 20:59:18: 26000000 INFO @ Fri, 10 Dec 2021 20:59:25: 32000000 INFO @ Fri, 10 Dec 2021 20:59:25: 21000000 INFO @ Fri, 10 Dec 2021 20:59:25: 27000000 INFO @ Fri, 10 Dec 2021 20:59:32: 33000000 INFO @ Fri, 10 Dec 2021 20:59:33: 28000000 INFO @ Fri, 10 Dec 2021 20:59:34: 22000000 INFO @ Fri, 10 Dec 2021 20:59:40: 34000000 INFO @ Fri, 10 Dec 2021 20:59:40: 29000000 INFO @ Fri, 10 Dec 2021 20:59:42: 23000000 INFO @ Fri, 10 Dec 2021 20:59:47: 35000000 INFO @ Fri, 10 Dec 2021 20:59:48: 30000000 INFO @ Fri, 10 Dec 2021 20:59:50: 24000000 INFO @ Fri, 10 Dec 2021 20:59:55: 36000000 INFO @ Fri, 10 Dec 2021 20:59:55: 31000000 INFO @ Fri, 10 Dec 2021 20:59:58: 25000000 INFO @ Fri, 10 Dec 2021 21:00:02: 37000000 INFO @ Fri, 10 Dec 2021 21:00:03: 32000000 INFO @ Fri, 10 Dec 2021 21:00:07: 26000000 INFO @ Fri, 10 Dec 2021 21:00:10: 38000000 INFO @ Fri, 10 Dec 2021 21:00:10: 33000000 INFO @ Fri, 10 Dec 2021 21:00:15: 27000000 INFO @ Fri, 10 Dec 2021 21:00:17: 39000000 INFO @ Fri, 10 Dec 2021 21:00:18: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 21:00:23: 28000000 INFO @ Fri, 10 Dec 2021 21:00:25: 40000000 INFO @ Fri, 10 Dec 2021 21:00:25: 35000000 INFO @ Fri, 10 Dec 2021 21:00:31: 29000000 INFO @ Fri, 10 Dec 2021 21:00:33: 41000000 INFO @ Fri, 10 Dec 2021 21:00:33: 36000000 INFO @ Fri, 10 Dec 2021 21:00:39: 30000000 INFO @ Fri, 10 Dec 2021 21:00:40: 37000000 INFO @ Fri, 10 Dec 2021 21:00:40: 42000000 INFO @ Fri, 10 Dec 2021 21:00:47: 31000000 INFO @ Fri, 10 Dec 2021 21:00:48: 38000000 INFO @ Fri, 10 Dec 2021 21:00:48: 43000000 INFO @ Fri, 10 Dec 2021 21:00:55: 39000000 INFO @ Fri, 10 Dec 2021 21:00:55: 44000000 INFO @ Fri, 10 Dec 2021 21:00:56: 32000000 INFO @ Fri, 10 Dec 2021 21:01:03: 40000000 INFO @ Fri, 10 Dec 2021 21:01:03: 45000000 INFO @ Fri, 10 Dec 2021 21:01:04: 33000000 INFO @ Fri, 10 Dec 2021 21:01:10: 41000000 INFO @ Fri, 10 Dec 2021 21:01:10: 46000000 INFO @ Fri, 10 Dec 2021 21:01:12: 34000000 INFO @ Fri, 10 Dec 2021 21:01:18: 42000000 INFO @ Fri, 10 Dec 2021 21:01:18: 47000000 INFO @ Fri, 10 Dec 2021 21:01:20: 35000000 INFO @ Fri, 10 Dec 2021 21:01:25: 43000000 INFO @ Fri, 10 Dec 2021 21:01:26: 48000000 INFO @ Fri, 10 Dec 2021 21:01:28: 36000000 INFO @ Fri, 10 Dec 2021 21:01:33: 44000000 INFO @ Fri, 10 Dec 2021 21:01:33: 49000000 INFO @ Fri, 10 Dec 2021 21:01:36: 37000000 INFO @ Fri, 10 Dec 2021 21:01:40: 50000000 INFO @ Fri, 10 Dec 2021 21:01:40: 45000000 INFO @ Fri, 10 Dec 2021 21:01:44: 38000000 INFO @ Fri, 10 Dec 2021 21:01:48: 51000000 INFO @ Fri, 10 Dec 2021 21:01:48: 46000000 INFO @ Fri, 10 Dec 2021 21:01:52: 39000000 INFO @ Fri, 10 Dec 2021 21:01:55: 52000000 INFO @ Fri, 10 Dec 2021 21:01:55: 47000000 INFO @ Fri, 10 Dec 2021 21:02:00: 40000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 21:02:03: 53000000 INFO @ Fri, 10 Dec 2021 21:02:03: 48000000 INFO @ Fri, 10 Dec 2021 21:02:08: 41000000 INFO @ Fri, 10 Dec 2021 21:02:10: 54000000 INFO @ Fri, 10 Dec 2021 21:02:11: 49000000 INFO @ Fri, 10 Dec 2021 21:02:12: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 21:02:12: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 21:02:12: #1 total tags in treatment: 54152657 INFO @ Fri, 10 Dec 2021 21:02:12: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:02:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:02:13: #1 tags after filtering in treatment: 54152657 INFO @ Fri, 10 Dec 2021 21:02:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:02:13: #1 finished! INFO @ Fri, 10 Dec 2021 21:02:13: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:02:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:02:16: 42000000 INFO @ Fri, 10 Dec 2021 21:02:16: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 21:02:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:02:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:02:18: 50000000 INFO @ Fri, 10 Dec 2021 21:02:24: 43000000 INFO @ Fri, 10 Dec 2021 21:02:25: 51000000 INFO @ Fri, 10 Dec 2021 21:02:32: 44000000 INFO @ Fri, 10 Dec 2021 21:02:32: 52000000 INFO @ Fri, 10 Dec 2021 21:02:40: 53000000 INFO @ Fri, 10 Dec 2021 21:02:40: 45000000 INFO @ Fri, 10 Dec 2021 21:02:47: 54000000 INFO @ Fri, 10 Dec 2021 21:02:48: 46000000 INFO @ Fri, 10 Dec 2021 21:02:48: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 21:02:48: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 21:02:48: #1 total tags in treatment: 54152657 INFO @ Fri, 10 Dec 2021 21:02:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:02:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:02:49: #1 tags after filtering in treatment: 54152657 INFO @ Fri, 10 Dec 2021 21:02:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:02:49: #1 finished! INFO @ Fri, 10 Dec 2021 21:02:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:02:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:02:52: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 21:02:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:02:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:02:55: 47000000 INFO @ Fri, 10 Dec 2021 21:03:03: 48000000 INFO @ Fri, 10 Dec 2021 21:03:10: 49000000 INFO @ Fri, 10 Dec 2021 21:03:17: 50000000 INFO @ Fri, 10 Dec 2021 21:03:24: 51000000 INFO @ Fri, 10 Dec 2021 21:03:32: 52000000 INFO @ Fri, 10 Dec 2021 21:03:39: 53000000 INFO @ Fri, 10 Dec 2021 21:03:46: 54000000 INFO @ Fri, 10 Dec 2021 21:03:47: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 21:03:47: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 21:03:47: #1 total tags in treatment: 54152657 INFO @ Fri, 10 Dec 2021 21:03:47: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:03:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:03:48: #1 tags after filtering in treatment: 54152657 INFO @ Fri, 10 Dec 2021 21:03:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 21:03:48: #1 finished! INFO @ Fri, 10 Dec 2021 21:03:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:03:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:03:52: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 21:03:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 21:03:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910771/SRX9910771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling