Job ID = 14168762 SRX = SRX9910770 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 50137796 spots for SRR13498841/SRR13498841.sra Written 50137796 spots for SRR13498841/SRR13498841.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169856 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:32 50137796 reads; of these: 50137796 (100.00%) were unpaired; of these: 10325907 (20.60%) aligned 0 times 28489641 (56.82%) aligned exactly 1 time 11322248 (22.58%) aligned >1 times 79.40% overall alignment rate Time searching: 00:31:32 Overall time: 00:31:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 21658030 / 39811889 = 0.5440 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:48:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:48:02: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:48:02: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:48:11: 1000000 INFO @ Fri, 10 Dec 2021 20:48:20: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:48:29: 3000000 INFO @ Fri, 10 Dec 2021 20:48:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:48:31: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:48:31: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:48:37: 4000000 INFO @ Fri, 10 Dec 2021 20:48:40: 1000000 INFO @ Fri, 10 Dec 2021 20:48:46: 5000000 INFO @ Fri, 10 Dec 2021 20:48:49: 2000000 INFO @ Fri, 10 Dec 2021 20:48:55: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:48:58: 3000000 INFO @ Fri, 10 Dec 2021 20:49:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:49:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:49:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:49:04: 7000000 INFO @ Fri, 10 Dec 2021 20:49:08: 4000000 INFO @ Fri, 10 Dec 2021 20:49:10: 1000000 INFO @ Fri, 10 Dec 2021 20:49:13: 8000000 INFO @ Fri, 10 Dec 2021 20:49:18: 5000000 INFO @ Fri, 10 Dec 2021 20:49:20: 2000000 INFO @ Fri, 10 Dec 2021 20:49:23: 9000000 INFO @ Fri, 10 Dec 2021 20:49:29: 6000000 INFO @ Fri, 10 Dec 2021 20:49:30: 3000000 INFO @ Fri, 10 Dec 2021 20:49:32: 10000000 INFO @ Fri, 10 Dec 2021 20:49:38: 7000000 INFO @ Fri, 10 Dec 2021 20:49:40: 4000000 INFO @ Fri, 10 Dec 2021 20:49:41: 11000000 INFO @ Fri, 10 Dec 2021 20:49:48: 8000000 INFO @ Fri, 10 Dec 2021 20:49:49: 5000000 INFO @ Fri, 10 Dec 2021 20:49:50: 12000000 INFO @ Fri, 10 Dec 2021 20:49:58: 9000000 INFO @ Fri, 10 Dec 2021 20:49:59: 6000000 INFO @ Fri, 10 Dec 2021 20:50:00: 13000000 INFO @ Fri, 10 Dec 2021 20:50:07: 10000000 INFO @ Fri, 10 Dec 2021 20:50:09: 7000000 INFO @ Fri, 10 Dec 2021 20:50:09: 14000000 INFO @ Fri, 10 Dec 2021 20:50:17: 11000000 INFO @ Fri, 10 Dec 2021 20:50:18: 8000000 INFO @ Fri, 10 Dec 2021 20:50:19: 15000000 INFO @ Fri, 10 Dec 2021 20:50:28: 12000000 INFO @ Fri, 10 Dec 2021 20:50:28: 16000000 INFO @ Fri, 10 Dec 2021 20:50:29: 9000000 INFO @ Fri, 10 Dec 2021 20:50:37: 17000000 INFO @ Fri, 10 Dec 2021 20:50:38: 13000000 INFO @ Fri, 10 Dec 2021 20:50:38: 10000000 INFO @ Fri, 10 Dec 2021 20:50:46: 18000000 INFO @ Fri, 10 Dec 2021 20:50:48: 14000000 INFO @ Fri, 10 Dec 2021 20:50:48: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:50:48: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:50:48: #1 total tags in treatment: 18153859 INFO @ Fri, 10 Dec 2021 20:50:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:50:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:50:48: #1 tags after filtering in treatment: 18153859 INFO @ Fri, 10 Dec 2021 20:50:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:50:48: #1 finished! INFO @ Fri, 10 Dec 2021 20:50:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:50:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:50:48: 11000000 INFO @ Fri, 10 Dec 2021 20:50:50: #2 number of paired peaks: 1286 INFO @ Fri, 10 Dec 2021 20:50:50: start model_add_line... INFO @ Fri, 10 Dec 2021 20:50:50: start X-correlation... INFO @ Fri, 10 Dec 2021 20:50:50: end of X-cor INFO @ Fri, 10 Dec 2021 20:50:50: #2 finished! INFO @ Fri, 10 Dec 2021 20:50:50: #2 predicted fragment length is 236 bps INFO @ Fri, 10 Dec 2021 20:50:50: #2 alternative fragment length(s) may be 236 bps INFO @ Fri, 10 Dec 2021 20:50:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.05_model.r INFO @ Fri, 10 Dec 2021 20:50:50: #3 Call peaks... INFO @ Fri, 10 Dec 2021 20:50:50: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 20:50:57: 15000000 INFO @ Fri, 10 Dec 2021 20:50:58: 12000000 INFO @ Fri, 10 Dec 2021 20:51:07: 16000000 INFO @ Fri, 10 Dec 2021 20:51:08: 13000000 INFO @ Fri, 10 Dec 2021 20:51:17: 17000000 INFO @ Fri, 10 Dec 2021 20:51:18: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 20:51:27: 18000000 INFO @ Fri, 10 Dec 2021 20:51:28: 15000000 INFO @ Fri, 10 Dec 2021 20:51:29: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:51:29: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:51:29: #1 total tags in treatment: 18153859 INFO @ Fri, 10 Dec 2021 20:51:29: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:51:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:51:29: #1 tags after filtering in treatment: 18153859 INFO @ Fri, 10 Dec 2021 20:51:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:51:29: #1 finished! INFO @ Fri, 10 Dec 2021 20:51:29: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:51:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:51:31: #2 number of paired peaks: 1286 INFO @ Fri, 10 Dec 2021 20:51:31: start model_add_line... INFO @ Fri, 10 Dec 2021 20:51:31: start X-correlation... INFO @ Fri, 10 Dec 2021 20:51:31: end of X-cor INFO @ Fri, 10 Dec 2021 20:51:31: #2 finished! INFO @ Fri, 10 Dec 2021 20:51:31: #2 predicted fragment length is 236 bps INFO @ Fri, 10 Dec 2021 20:51:31: #2 alternative fragment length(s) may be 236 bps INFO @ Fri, 10 Dec 2021 20:51:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.10_model.r INFO @ Fri, 10 Dec 2021 20:51:31: #3 Call peaks... INFO @ Fri, 10 Dec 2021 20:51:31: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 20:51:37: 16000000 INFO @ Fri, 10 Dec 2021 20:51:46: 17000000 INFO @ Fri, 10 Dec 2021 20:51:51: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 20:51:56: 18000000 INFO @ Fri, 10 Dec 2021 20:51:57: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:51:57: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:51:57: #1 total tags in treatment: 18153859 INFO @ Fri, 10 Dec 2021 20:51:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:51:58: #1 tags after filtering in treatment: 18153859 INFO @ Fri, 10 Dec 2021 20:51:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:51:58: #1 finished! INFO @ Fri, 10 Dec 2021 20:51:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:51:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:51:59: #2 number of paired peaks: 1286 INFO @ Fri, 10 Dec 2021 20:51:59: start model_add_line... INFO @ Fri, 10 Dec 2021 20:52:00: start X-correlation... INFO @ Fri, 10 Dec 2021 20:52:00: end of X-cor INFO @ Fri, 10 Dec 2021 20:52:00: #2 finished! INFO @ Fri, 10 Dec 2021 20:52:00: #2 predicted fragment length is 236 bps INFO @ Fri, 10 Dec 2021 20:52:00: #2 alternative fragment length(s) may be 236 bps INFO @ Fri, 10 Dec 2021 20:52:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.20_model.r INFO @ Fri, 10 Dec 2021 20:52:00: #3 Call peaks... INFO @ Fri, 10 Dec 2021 20:52:00: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 20:52:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.05_peaks.xls INFO @ Fri, 10 Dec 2021 20:52:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 20:52:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.05_summits.bed INFO @ Fri, 10 Dec 2021 20:52:19: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (9691 records, 4 fields): 24 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:52:30: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 20:52:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.10_peaks.xls INFO @ Fri, 10 Dec 2021 20:52:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 20:52:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.10_summits.bed INFO @ Fri, 10 Dec 2021 20:52:58: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6930 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:52:58: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 20:53:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.20_peaks.xls INFO @ Fri, 10 Dec 2021 20:53:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 20:53:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9910770/SRX9910770.20_summits.bed INFO @ Fri, 10 Dec 2021 20:53:25: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4525 records, 4 fields): 10 millis CompletedMACS2peakCalling