Job ID = 14168685 SRX = SRX9910769 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 75407323 spots for SRR13498840/SRR13498840.sra Written 75407323 spots for SRR13498840/SRR13498840.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169829 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:39:01 75407323 reads; of these: 75407323 (100.00%) were unpaired; of these: 6372065 (8.45%) aligned 0 times 45996523 (61.00%) aligned exactly 1 time 23038735 (30.55%) aligned >1 times 91.55% overall alignment rate Time searching: 00:39:02 Overall time: 00:39:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 14056312 / 69035258 = 0.2036 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:29:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:29:46: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:29:46: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:29:54: 1000000 INFO @ Fri, 10 Dec 2021 20:30:01: 2000000 INFO @ Fri, 10 Dec 2021 20:30:08: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:30:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:30:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:30:16: 4000000 INFO @ Fri, 10 Dec 2021 20:30:23: 1000000 INFO @ Fri, 10 Dec 2021 20:30:24: 5000000 INFO @ Fri, 10 Dec 2021 20:30:31: 2000000 INFO @ Fri, 10 Dec 2021 20:30:32: 6000000 INFO @ Fri, 10 Dec 2021 20:30:39: 3000000 INFO @ Fri, 10 Dec 2021 20:30:40: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:30:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:30:46: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:30:46: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:30:47: 4000000 INFO @ Fri, 10 Dec 2021 20:30:48: 8000000 INFO @ Fri, 10 Dec 2021 20:30:54: 1000000 INFO @ Fri, 10 Dec 2021 20:30:56: 5000000 INFO @ Fri, 10 Dec 2021 20:30:56: 9000000 INFO @ Fri, 10 Dec 2021 20:31:02: 2000000 INFO @ Fri, 10 Dec 2021 20:31:04: 6000000 INFO @ Fri, 10 Dec 2021 20:31:04: 10000000 INFO @ Fri, 10 Dec 2021 20:31:11: 3000000 INFO @ Fri, 10 Dec 2021 20:31:12: 7000000 INFO @ Fri, 10 Dec 2021 20:31:13: 11000000 INFO @ Fri, 10 Dec 2021 20:31:19: 4000000 INFO @ Fri, 10 Dec 2021 20:31:21: 8000000 INFO @ Fri, 10 Dec 2021 20:31:21: 12000000 INFO @ Fri, 10 Dec 2021 20:31:27: 5000000 INFO @ Fri, 10 Dec 2021 20:31:29: 9000000 INFO @ Fri, 10 Dec 2021 20:31:30: 13000000 INFO @ Fri, 10 Dec 2021 20:31:35: 6000000 INFO @ Fri, 10 Dec 2021 20:31:37: 10000000 INFO @ Fri, 10 Dec 2021 20:31:38: 14000000 INFO @ Fri, 10 Dec 2021 20:31:44: 7000000 INFO @ Fri, 10 Dec 2021 20:31:46: 11000000 INFO @ Fri, 10 Dec 2021 20:31:47: 15000000 INFO @ Fri, 10 Dec 2021 20:31:52: 8000000 INFO @ Fri, 10 Dec 2021 20:31:54: 12000000 INFO @ Fri, 10 Dec 2021 20:31:55: 16000000 INFO @ Fri, 10 Dec 2021 20:32:00: 9000000 INFO @ Fri, 10 Dec 2021 20:32:02: 13000000 INFO @ Fri, 10 Dec 2021 20:32:04: 17000000 INFO @ Fri, 10 Dec 2021 20:32:08: 10000000 INFO @ Fri, 10 Dec 2021 20:32:10: 14000000 INFO @ Fri, 10 Dec 2021 20:32:12: 18000000 INFO @ Fri, 10 Dec 2021 20:32:16: 11000000 INFO @ Fri, 10 Dec 2021 20:32:19: 15000000 INFO @ Fri, 10 Dec 2021 20:32:21: 19000000 INFO @ Fri, 10 Dec 2021 20:32:25: 12000000 INFO @ Fri, 10 Dec 2021 20:32:27: 16000000 INFO @ Fri, 10 Dec 2021 20:32:30: 20000000 INFO @ Fri, 10 Dec 2021 20:32:33: 13000000 INFO @ Fri, 10 Dec 2021 20:32:35: 17000000 INFO @ Fri, 10 Dec 2021 20:32:38: 21000000 INFO @ Fri, 10 Dec 2021 20:32:41: 14000000 INFO @ Fri, 10 Dec 2021 20:32:43: 18000000 INFO @ Fri, 10 Dec 2021 20:32:46: 22000000 INFO @ Fri, 10 Dec 2021 20:32:49: 15000000 INFO @ Fri, 10 Dec 2021 20:32:52: 19000000 INFO @ Fri, 10 Dec 2021 20:32:55: 23000000 INFO @ Fri, 10 Dec 2021 20:32:58: 16000000 INFO @ Fri, 10 Dec 2021 20:33:00: 20000000 INFO @ Fri, 10 Dec 2021 20:33:03: 24000000 INFO @ Fri, 10 Dec 2021 20:33:06: 17000000 INFO @ Fri, 10 Dec 2021 20:33:08: 21000000 INFO @ Fri, 10 Dec 2021 20:33:12: 25000000 INFO @ Fri, 10 Dec 2021 20:33:14: 18000000 INFO @ Fri, 10 Dec 2021 20:33:17: 22000000 INFO @ Fri, 10 Dec 2021 20:33:20: 26000000 INFO @ Fri, 10 Dec 2021 20:33:23: 19000000 INFO @ Fri, 10 Dec 2021 20:33:25: 23000000 INFO @ Fri, 10 Dec 2021 20:33:28: 27000000 INFO @ Fri, 10 Dec 2021 20:33:31: 20000000 INFO @ Fri, 10 Dec 2021 20:33:33: 24000000 INFO @ Fri, 10 Dec 2021 20:33:37: 28000000 INFO @ Fri, 10 Dec 2021 20:33:39: 21000000 INFO @ Fri, 10 Dec 2021 20:33:42: 25000000 INFO @ Fri, 10 Dec 2021 20:33:45: 29000000 INFO @ Fri, 10 Dec 2021 20:33:48: 22000000 INFO @ Fri, 10 Dec 2021 20:33:50: 26000000 INFO @ Fri, 10 Dec 2021 20:33:53: 30000000 INFO @ Fri, 10 Dec 2021 20:33:56: 23000000 INFO @ Fri, 10 Dec 2021 20:33:58: 27000000 INFO @ Fri, 10 Dec 2021 20:34:01: 31000000 INFO @ Fri, 10 Dec 2021 20:34:04: 24000000 INFO @ Fri, 10 Dec 2021 20:34:06: 28000000 INFO @ Fri, 10 Dec 2021 20:34:09: 32000000 INFO @ Fri, 10 Dec 2021 20:34:13: 25000000 INFO @ Fri, 10 Dec 2021 20:34:14: 29000000 INFO @ Fri, 10 Dec 2021 20:34:18: 33000000 INFO @ Fri, 10 Dec 2021 20:34:21: 26000000 INFO @ Fri, 10 Dec 2021 20:34:23: 30000000 INFO @ Fri, 10 Dec 2021 20:34:26: 34000000 INFO @ Fri, 10 Dec 2021 20:34:29: 27000000 INFO @ Fri, 10 Dec 2021 20:34:31: 31000000 INFO @ Fri, 10 Dec 2021 20:34:34: 35000000 INFO @ Fri, 10 Dec 2021 20:34:37: 28000000 INFO @ Fri, 10 Dec 2021 20:34:39: 32000000 INFO @ Fri, 10 Dec 2021 20:34:42: 36000000 INFO @ Fri, 10 Dec 2021 20:34:45: 29000000 INFO @ Fri, 10 Dec 2021 20:34:47: 33000000 INFO @ Fri, 10 Dec 2021 20:34:50: 37000000 INFO @ Fri, 10 Dec 2021 20:34:54: 30000000 INFO @ Fri, 10 Dec 2021 20:34:55: 34000000 INFO @ Fri, 10 Dec 2021 20:34:59: 38000000 INFO @ Fri, 10 Dec 2021 20:35:02: 31000000 INFO @ Fri, 10 Dec 2021 20:35:04: 35000000 INFO @ Fri, 10 Dec 2021 20:35:07: 39000000 INFO @ Fri, 10 Dec 2021 20:35:10: 32000000 INFO @ Fri, 10 Dec 2021 20:35:12: 36000000 INFO @ Fri, 10 Dec 2021 20:35:15: 40000000 INFO @ Fri, 10 Dec 2021 20:35:18: 33000000 INFO @ Fri, 10 Dec 2021 20:35:20: 37000000 INFO @ Fri, 10 Dec 2021 20:35:24: 41000000 INFO @ Fri, 10 Dec 2021 20:35:27: 34000000 INFO @ Fri, 10 Dec 2021 20:35:28: 38000000 INFO @ Fri, 10 Dec 2021 20:35:32: 42000000 INFO @ Fri, 10 Dec 2021 20:35:35: 35000000 INFO @ Fri, 10 Dec 2021 20:35:36: 39000000 INFO @ Fri, 10 Dec 2021 20:35:40: 43000000 INFO @ Fri, 10 Dec 2021 20:35:43: 36000000 INFO @ Fri, 10 Dec 2021 20:35:45: 40000000 INFO @ Fri, 10 Dec 2021 20:35:48: 44000000 INFO @ Fri, 10 Dec 2021 20:35:51: 37000000 INFO @ Fri, 10 Dec 2021 20:35:53: 41000000 INFO @ Fri, 10 Dec 2021 20:35:56: 45000000 INFO @ Fri, 10 Dec 2021 20:36:00: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 20:36:01: 42000000 INFO @ Fri, 10 Dec 2021 20:36:05: 46000000 INFO @ Fri, 10 Dec 2021 20:36:08: 39000000 INFO @ Fri, 10 Dec 2021 20:36:10: 43000000 INFO @ Fri, 10 Dec 2021 20:36:13: 47000000 INFO @ Fri, 10 Dec 2021 20:36:16: 40000000 INFO @ Fri, 10 Dec 2021 20:36:18: 44000000 INFO @ Fri, 10 Dec 2021 20:36:21: 48000000 INFO @ Fri, 10 Dec 2021 20:36:24: 41000000 INFO @ Fri, 10 Dec 2021 20:36:26: 45000000 INFO @ Fri, 10 Dec 2021 20:36:29: 49000000 INFO @ Fri, 10 Dec 2021 20:36:33: 42000000 INFO @ Fri, 10 Dec 2021 20:36:34: 46000000 INFO @ Fri, 10 Dec 2021 20:36:38: 50000000 INFO @ Fri, 10 Dec 2021 20:36:41: 43000000 INFO @ Fri, 10 Dec 2021 20:36:43: 47000000 INFO @ Fri, 10 Dec 2021 20:36:46: 51000000 INFO @ Fri, 10 Dec 2021 20:36:49: 44000000 INFO @ Fri, 10 Dec 2021 20:36:51: 48000000 INFO @ Fri, 10 Dec 2021 20:36:54: 52000000 INFO @ Fri, 10 Dec 2021 20:36:58: 45000000 INFO @ Fri, 10 Dec 2021 20:36:59: 49000000 INFO @ Fri, 10 Dec 2021 20:37:02: 53000000 INFO @ Fri, 10 Dec 2021 20:37:06: 46000000 INFO @ Fri, 10 Dec 2021 20:37:07: 50000000 INFO @ Fri, 10 Dec 2021 20:37:11: 54000000 INFO @ Fri, 10 Dec 2021 20:37:14: 47000000 INFO @ Fri, 10 Dec 2021 20:37:16: 51000000 INFO @ Fri, 10 Dec 2021 20:37:19: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:37:19: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:37:19: #1 total tags in treatment: 54978946 INFO @ Fri, 10 Dec 2021 20:37:19: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:37:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:37:20: #1 tags after filtering in treatment: 54978946 INFO @ Fri, 10 Dec 2021 20:37:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:37:20: #1 finished! INFO @ Fri, 10 Dec 2021 20:37:20: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:37:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:37:22: 48000000 INFO @ Fri, 10 Dec 2021 20:37:24: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 20:37:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 20:37:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 10 Dec 2021 20:37:24: 52000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:37:30: 49000000 INFO @ Fri, 10 Dec 2021 20:37:32: 53000000 INFO @ Fri, 10 Dec 2021 20:37:38: 50000000 INFO @ Fri, 10 Dec 2021 20:37:40: 54000000 INFO @ Fri, 10 Dec 2021 20:37:46: 51000000 INFO @ Fri, 10 Dec 2021 20:37:48: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:37:48: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:37:48: #1 total tags in treatment: 54978946 INFO @ Fri, 10 Dec 2021 20:37:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:37:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:37:49: #1 tags after filtering in treatment: 54978946 INFO @ Fri, 10 Dec 2021 20:37:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:37:49: #1 finished! INFO @ Fri, 10 Dec 2021 20:37:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:37:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:37:52: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 20:37:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 20:37:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:37:54: 52000000 INFO @ Fri, 10 Dec 2021 20:38:01: 53000000 INFO @ Fri, 10 Dec 2021 20:38:08: 54000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 20:38:16: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:38:16: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:38:16: #1 total tags in treatment: 54978946 INFO @ Fri, 10 Dec 2021 20:38:16: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:38:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:38:17: #1 tags after filtering in treatment: 54978946 INFO @ Fri, 10 Dec 2021 20:38:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:38:17: #1 finished! INFO @ Fri, 10 Dec 2021 20:38:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:38:21: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 20:38:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 20:38:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910769/SRX9910769.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling