Job ID = 14168682 SRX = SRX9910767 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 56637642 spots for SRR13498838/SRR13498838.sra Written 56637642 spots for SRR13498838/SRR13498838.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169799 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:42 56637642 reads; of these: 56637642 (100.00%) were unpaired; of these: 5426376 (9.58%) aligned 0 times 36076441 (63.70%) aligned exactly 1 time 15134825 (26.72%) aligned >1 times 90.42% overall alignment rate Time searching: 00:25:42 Overall time: 00:25:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9269606 / 51211266 = 0.1810 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:01:22: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:01:22: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:01:29: 1000000 INFO @ Fri, 10 Dec 2021 20:01:37: 2000000 INFO @ Fri, 10 Dec 2021 20:01:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:01:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:01:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:01:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:01:52: 4000000 INFO @ Fri, 10 Dec 2021 20:02:00: 1000000 INFO @ Fri, 10 Dec 2021 20:02:01: 5000000 INFO @ Fri, 10 Dec 2021 20:02:08: 2000000 INFO @ Fri, 10 Dec 2021 20:02:09: 6000000 INFO @ Fri, 10 Dec 2021 20:02:15: 3000000 INFO @ Fri, 10 Dec 2021 20:02:18: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 20:02:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 20:02:22: #1 read tag files... INFO @ Fri, 10 Dec 2021 20:02:22: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 20:02:23: 4000000 INFO @ Fri, 10 Dec 2021 20:02:26: 8000000 INFO @ Fri, 10 Dec 2021 20:02:30: 1000000 INFO @ Fri, 10 Dec 2021 20:02:31: 5000000 INFO @ Fri, 10 Dec 2021 20:02:35: 9000000 INFO @ Fri, 10 Dec 2021 20:02:39: 2000000 INFO @ Fri, 10 Dec 2021 20:02:40: 6000000 INFO @ Fri, 10 Dec 2021 20:02:43: 10000000 INFO @ Fri, 10 Dec 2021 20:02:48: 7000000 INFO @ Fri, 10 Dec 2021 20:02:48: 3000000 INFO @ Fri, 10 Dec 2021 20:02:52: 11000000 INFO @ Fri, 10 Dec 2021 20:02:56: 8000000 INFO @ Fri, 10 Dec 2021 20:02:57: 4000000 INFO @ Fri, 10 Dec 2021 20:03:00: 12000000 INFO @ Fri, 10 Dec 2021 20:03:04: 9000000 INFO @ Fri, 10 Dec 2021 20:03:06: 5000000 INFO @ Fri, 10 Dec 2021 20:03:09: 13000000 INFO @ Fri, 10 Dec 2021 20:03:12: 10000000 INFO @ Fri, 10 Dec 2021 20:03:15: 6000000 INFO @ Fri, 10 Dec 2021 20:03:18: 14000000 INFO @ Fri, 10 Dec 2021 20:03:20: 11000000 INFO @ Fri, 10 Dec 2021 20:03:24: 7000000 INFO @ Fri, 10 Dec 2021 20:03:27: 15000000 INFO @ Fri, 10 Dec 2021 20:03:28: 12000000 INFO @ Fri, 10 Dec 2021 20:03:33: 8000000 INFO @ Fri, 10 Dec 2021 20:03:35: 16000000 INFO @ Fri, 10 Dec 2021 20:03:36: 13000000 INFO @ Fri, 10 Dec 2021 20:03:41: 9000000 INFO @ Fri, 10 Dec 2021 20:03:43: 14000000 INFO @ Fri, 10 Dec 2021 20:03:44: 17000000 INFO @ Fri, 10 Dec 2021 20:03:50: 10000000 INFO @ Fri, 10 Dec 2021 20:03:51: 15000000 INFO @ Fri, 10 Dec 2021 20:03:53: 18000000 INFO @ Fri, 10 Dec 2021 20:03:58: 11000000 INFO @ Fri, 10 Dec 2021 20:03:59: 16000000 INFO @ Fri, 10 Dec 2021 20:04:01: 19000000 INFO @ Fri, 10 Dec 2021 20:04:07: 12000000 INFO @ Fri, 10 Dec 2021 20:04:07: 17000000 INFO @ Fri, 10 Dec 2021 20:04:10: 20000000 INFO @ Fri, 10 Dec 2021 20:04:15: 18000000 INFO @ Fri, 10 Dec 2021 20:04:15: 13000000 INFO @ Fri, 10 Dec 2021 20:04:18: 21000000 INFO @ Fri, 10 Dec 2021 20:04:23: 19000000 INFO @ Fri, 10 Dec 2021 20:04:24: 14000000 INFO @ Fri, 10 Dec 2021 20:04:27: 22000000 INFO @ Fri, 10 Dec 2021 20:04:30: 20000000 INFO @ Fri, 10 Dec 2021 20:04:32: 15000000 INFO @ Fri, 10 Dec 2021 20:04:35: 23000000 INFO @ Fri, 10 Dec 2021 20:04:39: 21000000 INFO @ Fri, 10 Dec 2021 20:04:41: 16000000 INFO @ Fri, 10 Dec 2021 20:04:44: 24000000 INFO @ Fri, 10 Dec 2021 20:04:46: 22000000 INFO @ Fri, 10 Dec 2021 20:04:50: 17000000 INFO @ Fri, 10 Dec 2021 20:04:52: 25000000 INFO @ Fri, 10 Dec 2021 20:04:54: 23000000 INFO @ Fri, 10 Dec 2021 20:04:58: 18000000 INFO @ Fri, 10 Dec 2021 20:05:01: 26000000 INFO @ Fri, 10 Dec 2021 20:05:02: 24000000 INFO @ Fri, 10 Dec 2021 20:05:07: 19000000 INFO @ Fri, 10 Dec 2021 20:05:09: 27000000 INFO @ Fri, 10 Dec 2021 20:05:10: 25000000 INFO @ Fri, 10 Dec 2021 20:05:16: 20000000 INFO @ Fri, 10 Dec 2021 20:05:17: 26000000 INFO @ Fri, 10 Dec 2021 20:05:18: 28000000 INFO @ Fri, 10 Dec 2021 20:05:25: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 20:05:25: 27000000 INFO @ Fri, 10 Dec 2021 20:05:27: 29000000 INFO @ Fri, 10 Dec 2021 20:05:33: 22000000 INFO @ Fri, 10 Dec 2021 20:05:33: 28000000 INFO @ Fri, 10 Dec 2021 20:05:36: 30000000 INFO @ Fri, 10 Dec 2021 20:05:42: 29000000 INFO @ Fri, 10 Dec 2021 20:05:42: 23000000 INFO @ Fri, 10 Dec 2021 20:05:44: 31000000 INFO @ Fri, 10 Dec 2021 20:05:50: 30000000 INFO @ Fri, 10 Dec 2021 20:05:50: 24000000 INFO @ Fri, 10 Dec 2021 20:05:53: 32000000 INFO @ Fri, 10 Dec 2021 20:05:58: 31000000 INFO @ Fri, 10 Dec 2021 20:05:58: 25000000 INFO @ Fri, 10 Dec 2021 20:06:02: 33000000 INFO @ Fri, 10 Dec 2021 20:06:06: 32000000 INFO @ Fri, 10 Dec 2021 20:06:07: 26000000 INFO @ Fri, 10 Dec 2021 20:06:11: 34000000 INFO @ Fri, 10 Dec 2021 20:06:14: 33000000 INFO @ Fri, 10 Dec 2021 20:06:15: 27000000 INFO @ Fri, 10 Dec 2021 20:06:20: 35000000 INFO @ Fri, 10 Dec 2021 20:06:22: 34000000 INFO @ Fri, 10 Dec 2021 20:06:24: 28000000 INFO @ Fri, 10 Dec 2021 20:06:29: 36000000 INFO @ Fri, 10 Dec 2021 20:06:30: 35000000 INFO @ Fri, 10 Dec 2021 20:06:33: 29000000 INFO @ Fri, 10 Dec 2021 20:06:37: 37000000 INFO @ Fri, 10 Dec 2021 20:06:38: 36000000 INFO @ Fri, 10 Dec 2021 20:06:42: 30000000 INFO @ Fri, 10 Dec 2021 20:06:46: 37000000 INFO @ Fri, 10 Dec 2021 20:06:46: 38000000 INFO @ Fri, 10 Dec 2021 20:06:51: 31000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 20:06:53: 38000000 INFO @ Fri, 10 Dec 2021 20:06:55: 39000000 INFO @ Fri, 10 Dec 2021 20:06:59: 32000000 INFO @ Fri, 10 Dec 2021 20:07:01: 39000000 INFO @ Fri, 10 Dec 2021 20:07:04: 40000000 INFO @ Fri, 10 Dec 2021 20:07:08: 33000000 INFO @ Fri, 10 Dec 2021 20:07:09: 40000000 INFO @ Fri, 10 Dec 2021 20:07:12: 41000000 INFO @ Fri, 10 Dec 2021 20:07:17: 41000000 INFO @ Fri, 10 Dec 2021 20:07:17: 34000000 INFO @ Fri, 10 Dec 2021 20:07:20: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:07:20: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:07:20: #1 total tags in treatment: 41941660 INFO @ Fri, 10 Dec 2021 20:07:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:07:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:07:21: #1 tags after filtering in treatment: 41941660 INFO @ Fri, 10 Dec 2021 20:07:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:07:21: #1 finished! INFO @ Fri, 10 Dec 2021 20:07:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:07:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:07:24: #2 number of paired peaks: 26 WARNING @ Fri, 10 Dec 2021 20:07:24: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 20:07:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:07:24: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:07:24: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:07:24: #1 total tags in treatment: 41941660 INFO @ Fri, 10 Dec 2021 20:07:24: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:07:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:07:25: #1 tags after filtering in treatment: 41941660 INFO @ Fri, 10 Dec 2021 20:07:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:07:25: #1 finished! INFO @ Fri, 10 Dec 2021 20:07:25: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:07:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:07:26: 35000000 INFO @ Fri, 10 Dec 2021 20:07:28: #2 number of paired peaks: 26 WARNING @ Fri, 10 Dec 2021 20:07:28: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 20:07:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 20:07:34: 36000000 INFO @ Fri, 10 Dec 2021 20:07:42: 37000000 INFO @ Fri, 10 Dec 2021 20:07:50: 38000000 INFO @ Fri, 10 Dec 2021 20:07:58: 39000000 INFO @ Fri, 10 Dec 2021 20:08:05: 40000000 INFO @ Fri, 10 Dec 2021 20:08:13: 41000000 INFO @ Fri, 10 Dec 2021 20:08:21: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 20:08:21: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 20:08:21: #1 total tags in treatment: 41941660 INFO @ Fri, 10 Dec 2021 20:08:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 20:08:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 20:08:21: #1 tags after filtering in treatment: 41941660 INFO @ Fri, 10 Dec 2021 20:08:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 20:08:21: #1 finished! INFO @ Fri, 10 Dec 2021 20:08:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 20:08:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 20:08:24: #2 number of paired peaks: 26 WARNING @ Fri, 10 Dec 2021 20:08:24: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 20:08:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9910767/SRX9910767.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling