Job ID = 14167013 SRX = SRX9717947 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 44430266 spots for SRR13288854/SRR13288854.sra Written 44430266 spots for SRR13288854/SRR13288854.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167394 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:49 44430266 reads; of these: 44430266 (100.00%) were unpaired; of these: 7399885 (16.66%) aligned 0 times 27657176 (62.25%) aligned exactly 1 time 9373205 (21.10%) aligned >1 times 83.34% overall alignment rate Time searching: 00:18:49 Overall time: 00:18:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14962384 / 37030381 = 0.4041 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:17:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:17:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:17:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:17:40: 1000000 INFO @ Fri, 10 Dec 2021 09:17:45: 2000000 INFO @ Fri, 10 Dec 2021 09:17:50: 3000000 INFO @ Fri, 10 Dec 2021 09:17:56: 4000000 INFO @ Fri, 10 Dec 2021 09:18:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:18:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:18:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:18:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:18:07: 6000000 INFO @ Fri, 10 Dec 2021 09:18:10: 1000000 INFO @ Fri, 10 Dec 2021 09:18:13: 7000000 INFO @ Fri, 10 Dec 2021 09:18:15: 2000000 INFO @ Fri, 10 Dec 2021 09:18:18: 8000000 INFO @ Fri, 10 Dec 2021 09:18:21: 3000000 INFO @ Fri, 10 Dec 2021 09:18:23: 9000000 INFO @ Fri, 10 Dec 2021 09:18:26: 4000000 INFO @ Fri, 10 Dec 2021 09:18:29: 10000000 INFO @ Fri, 10 Dec 2021 09:18:32: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:18:34: 11000000 INFO @ Fri, 10 Dec 2021 09:18:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:18:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:18:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:18:37: 6000000 INFO @ Fri, 10 Dec 2021 09:18:40: 12000000 INFO @ Fri, 10 Dec 2021 09:18:41: 1000000 INFO @ Fri, 10 Dec 2021 09:18:43: 7000000 INFO @ Fri, 10 Dec 2021 09:18:46: 13000000 INFO @ Fri, 10 Dec 2021 09:18:46: 2000000 INFO @ Fri, 10 Dec 2021 09:18:48: 8000000 INFO @ Fri, 10 Dec 2021 09:18:51: 14000000 INFO @ Fri, 10 Dec 2021 09:18:52: 3000000 INFO @ Fri, 10 Dec 2021 09:18:54: 9000000 INFO @ Fri, 10 Dec 2021 09:18:57: 15000000 INFO @ Fri, 10 Dec 2021 09:18:57: 4000000 INFO @ Fri, 10 Dec 2021 09:18:59: 10000000 INFO @ Fri, 10 Dec 2021 09:19:02: 5000000 INFO @ Fri, 10 Dec 2021 09:19:03: 16000000 INFO @ Fri, 10 Dec 2021 09:19:05: 11000000 INFO @ Fri, 10 Dec 2021 09:19:08: 6000000 INFO @ Fri, 10 Dec 2021 09:19:08: 17000000 INFO @ Fri, 10 Dec 2021 09:19:10: 12000000 INFO @ Fri, 10 Dec 2021 09:19:13: 7000000 INFO @ Fri, 10 Dec 2021 09:19:15: 18000000 INFO @ Fri, 10 Dec 2021 09:19:16: 13000000 INFO @ Fri, 10 Dec 2021 09:19:19: 8000000 INFO @ Fri, 10 Dec 2021 09:19:21: 19000000 INFO @ Fri, 10 Dec 2021 09:19:22: 14000000 INFO @ Fri, 10 Dec 2021 09:19:25: 9000000 INFO @ Fri, 10 Dec 2021 09:19:27: 20000000 INFO @ Fri, 10 Dec 2021 09:19:28: 15000000 INFO @ Fri, 10 Dec 2021 09:19:30: 10000000 INFO @ Fri, 10 Dec 2021 09:19:33: 21000000 INFO @ Fri, 10 Dec 2021 09:19:34: 16000000 INFO @ Fri, 10 Dec 2021 09:19:36: 11000000 INFO @ Fri, 10 Dec 2021 09:19:39: 22000000 INFO @ Fri, 10 Dec 2021 09:19:39: 17000000 INFO @ Fri, 10 Dec 2021 09:19:40: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:19:40: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:19:40: #1 total tags in treatment: 22067997 INFO @ Fri, 10 Dec 2021 09:19:40: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:19:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:19:40: #1 tags after filtering in treatment: 22067997 INFO @ Fri, 10 Dec 2021 09:19:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:19:40: #1 finished! INFO @ Fri, 10 Dec 2021 09:19:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:19:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:19:42: 12000000 INFO @ Fri, 10 Dec 2021 09:19:42: #2 number of paired peaks: 11 WARNING @ Fri, 10 Dec 2021 09:19:42: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:19:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:19:45: 18000000 INFO @ Fri, 10 Dec 2021 09:19:47: 13000000 INFO @ Fri, 10 Dec 2021 09:19:51: 19000000 INFO @ Fri, 10 Dec 2021 09:19:53: 14000000 INFO @ Fri, 10 Dec 2021 09:19:56: 20000000 INFO @ Fri, 10 Dec 2021 09:19:58: 15000000 INFO @ Fri, 10 Dec 2021 09:20:02: 21000000 INFO @ Fri, 10 Dec 2021 09:20:04: 16000000 INFO @ Fri, 10 Dec 2021 09:20:08: 22000000 INFO @ Fri, 10 Dec 2021 09:20:08: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:20:08: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:20:08: #1 total tags in treatment: 22067997 INFO @ Fri, 10 Dec 2021 09:20:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:20:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:20:09: #1 tags after filtering in treatment: 22067997 INFO @ Fri, 10 Dec 2021 09:20:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:20:09: #1 finished! INFO @ Fri, 10 Dec 2021 09:20:09: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:20:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:20:09: 17000000 INFO @ Fri, 10 Dec 2021 09:20:10: #2 number of paired peaks: 11 WARNING @ Fri, 10 Dec 2021 09:20:10: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:20:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:20:15: 18000000 INFO @ Fri, 10 Dec 2021 09:20:20: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:20:25: 20000000 INFO @ Fri, 10 Dec 2021 09:20:31: 21000000 INFO @ Fri, 10 Dec 2021 09:20:36: 22000000 INFO @ Fri, 10 Dec 2021 09:20:37: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:20:37: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:20:37: #1 total tags in treatment: 22067997 INFO @ Fri, 10 Dec 2021 09:20:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:20:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:20:37: #1 tags after filtering in treatment: 22067997 INFO @ Fri, 10 Dec 2021 09:20:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:20:37: #1 finished! INFO @ Fri, 10 Dec 2021 09:20:37: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:20:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:20:39: #2 number of paired peaks: 11 WARNING @ Fri, 10 Dec 2021 09:20:39: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:20:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9717947/SRX9717947.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。