Job ID = 6528519 SRX = SRX969914 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:29:13 prefetch.2.10.7: 1) Downloading 'SRR1931630'... 2020-06-29T15:29:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:32:30 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:32:30 prefetch.2.10.7: 1) 'SRR1931630' was downloaded successfully Read 18510338 spots for SRR1931630/SRR1931630.sra Written 18510338 spots for SRR1931630/SRR1931630.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:25 18510338 reads; of these: 18510338 (100.00%) were unpaired; of these: 475227 (2.57%) aligned 0 times 13237651 (71.51%) aligned exactly 1 time 4797460 (25.92%) aligned >1 times 97.43% overall alignment rate Time searching: 00:06:25 Overall time: 00:06:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1833034 / 18035111 = 0.1016 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:51:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:51:07: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:51:07: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:51:13: 1000000 INFO @ Tue, 30 Jun 2020 00:51:19: 2000000 INFO @ Tue, 30 Jun 2020 00:51:25: 3000000 INFO @ Tue, 30 Jun 2020 00:51:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:51:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:51:37: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:51:37: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:51:39: 5000000 INFO @ Tue, 30 Jun 2020 00:51:44: 1000000 INFO @ Tue, 30 Jun 2020 00:51:45: 6000000 INFO @ Tue, 30 Jun 2020 00:51:50: 2000000 INFO @ Tue, 30 Jun 2020 00:51:52: 7000000 INFO @ Tue, 30 Jun 2020 00:51:57: 3000000 INFO @ Tue, 30 Jun 2020 00:51:58: 8000000 INFO @ Tue, 30 Jun 2020 00:52:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:52:06: 9000000 INFO @ Tue, 30 Jun 2020 00:52:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:52:07: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:52:07: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:52:11: 5000000 INFO @ Tue, 30 Jun 2020 00:52:12: 10000000 INFO @ Tue, 30 Jun 2020 00:52:15: 1000000 INFO @ Tue, 30 Jun 2020 00:52:18: 6000000 INFO @ Tue, 30 Jun 2020 00:52:20: 11000000 INFO @ Tue, 30 Jun 2020 00:52:23: 2000000 INFO @ Tue, 30 Jun 2020 00:52:26: 7000000 INFO @ Tue, 30 Jun 2020 00:52:28: 12000000 INFO @ Tue, 30 Jun 2020 00:52:31: 3000000 INFO @ Tue, 30 Jun 2020 00:52:35: 8000000 INFO @ Tue, 30 Jun 2020 00:52:37: 13000000 INFO @ Tue, 30 Jun 2020 00:52:39: 4000000 INFO @ Tue, 30 Jun 2020 00:52:43: 9000000 INFO @ Tue, 30 Jun 2020 00:52:44: 14000000 INFO @ Tue, 30 Jun 2020 00:52:47: 5000000 INFO @ Tue, 30 Jun 2020 00:52:50: 10000000 INFO @ Tue, 30 Jun 2020 00:52:52: 15000000 INFO @ Tue, 30 Jun 2020 00:52:54: 6000000 INFO @ Tue, 30 Jun 2020 00:52:57: 11000000 INFO @ Tue, 30 Jun 2020 00:52:59: 16000000 INFO @ Tue, 30 Jun 2020 00:53:00: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:53:00: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:53:00: #1 total tags in treatment: 16202077 INFO @ Tue, 30 Jun 2020 00:53:00: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:53:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:53:01: #1 tags after filtering in treatment: 16202077 INFO @ Tue, 30 Jun 2020 00:53:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:53:01: #1 finished! INFO @ Tue, 30 Jun 2020 00:53:01: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:53:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:53:02: #2 number of paired peaks: 63 WARNING @ Tue, 30 Jun 2020 00:53:02: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:53:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:53:02: 7000000 INFO @ Tue, 30 Jun 2020 00:53:04: 12000000 INFO @ Tue, 30 Jun 2020 00:53:09: 8000000 INFO @ Tue, 30 Jun 2020 00:53:11: 13000000 INFO @ Tue, 30 Jun 2020 00:53:16: 9000000 INFO @ Tue, 30 Jun 2020 00:53:17: 14000000 INFO @ Tue, 30 Jun 2020 00:53:23: 10000000 INFO @ Tue, 30 Jun 2020 00:53:24: 15000000 INFO @ Tue, 30 Jun 2020 00:53:30: 16000000 INFO @ Tue, 30 Jun 2020 00:53:30: 11000000 INFO @ Tue, 30 Jun 2020 00:53:31: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:53:31: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:53:31: #1 total tags in treatment: 16202077 INFO @ Tue, 30 Jun 2020 00:53:31: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:53:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:53:32: #1 tags after filtering in treatment: 16202077 INFO @ Tue, 30 Jun 2020 00:53:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:53:32: #1 finished! INFO @ Tue, 30 Jun 2020 00:53:32: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:53:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:53:33: #2 number of paired peaks: 63 WARNING @ Tue, 30 Jun 2020 00:53:33: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:53:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:53:36: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:53:43: 13000000 INFO @ Tue, 30 Jun 2020 00:53:50: 14000000 INFO @ Tue, 30 Jun 2020 00:53:56: 15000000 INFO @ Tue, 30 Jun 2020 00:54:02: 16000000 INFO @ Tue, 30 Jun 2020 00:54:04: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:54:04: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:54:04: #1 total tags in treatment: 16202077 INFO @ Tue, 30 Jun 2020 00:54:04: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:54:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:54:04: #1 tags after filtering in treatment: 16202077 INFO @ Tue, 30 Jun 2020 00:54:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:54:04: #1 finished! INFO @ Tue, 30 Jun 2020 00:54:04: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:54:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:54:05: #2 number of paired peaks: 63 WARNING @ Tue, 30 Jun 2020 00:54:05: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:54:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX969914/SRX969914.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。