Job ID = 14171438 SRX = SRX9689641 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 42680888 spots for SRR13258876/SRR13258876.sra Written 42680888 spots for SRR13258876/SRR13258876.sra Read 42535901 spots for SRR13258877/SRR13258877.sra Written 42535901 spots for SRR13258877/SRR13258877.sra Read 44003837 spots for SRR13258878/SRR13258878.sra Written 44003837 spots for SRR13258878/SRR13258878.sra Read 42756069 spots for SRR13258879/SRR13258879.sra Written 42756069 spots for SRR13258879/SRR13258879.sra fastq に変換しました。 bowtie でマッピング中... Your job 14173570 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 06:30:34 171976695 reads; of these: 171976695 (100.00%) were paired; of these: 138594494 (80.59%) aligned concordantly 0 times 24504955 (14.25%) aligned concordantly exactly 1 time 8877246 (5.16%) aligned concordantly >1 times ---- 138594494 pairs aligned concordantly 0 times; of these: 5138861 (3.71%) aligned discordantly 1 time ---- 133455633 pairs aligned 0 times concordantly or discordantly; of these: 266911266 mates make up the pairs; of these: 246045992 (92.18%) aligned 0 times 2072134 (0.78%) aligned exactly 1 time 18793140 (7.04%) aligned >1 times 28.47% overall alignment rate Time searching: 06:30:34 Overall time: 06:30:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 13560707 / 38060844 = 0.3563 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:08:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:08:46: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:08:46: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:08:56: 1000000 INFO @ Sat, 11 Dec 2021 19:09:06: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:09:14: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:09:14: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:09:16: 3000000 INFO @ Sat, 11 Dec 2021 19:09:23: 1000000 INFO @ Sat, 11 Dec 2021 19:09:25: 4000000 INFO @ Sat, 11 Dec 2021 19:09:32: 2000000 INFO @ Sat, 11 Dec 2021 19:09:35: 5000000 INFO @ Sat, 11 Dec 2021 19:09:41: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 19:09:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 19:09:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 19:09:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 19:09:45: 6000000 INFO @ Sat, 11 Dec 2021 19:09:49: 4000000 INFO @ Sat, 11 Dec 2021 19:09:53: 1000000 INFO @ Sat, 11 Dec 2021 19:09:55: 7000000 INFO @ Sat, 11 Dec 2021 19:09:58: 5000000 INFO @ Sat, 11 Dec 2021 19:10:02: 2000000 INFO @ Sat, 11 Dec 2021 19:10:04: 8000000 INFO @ Sat, 11 Dec 2021 19:10:07: 6000000 INFO @ Sat, 11 Dec 2021 19:10:11: 3000000 INFO @ Sat, 11 Dec 2021 19:10:14: 9000000 INFO @ Sat, 11 Dec 2021 19:10:15: 7000000 INFO @ Sat, 11 Dec 2021 19:10:19: 4000000 INFO @ Sat, 11 Dec 2021 19:10:23: 10000000 INFO @ Sat, 11 Dec 2021 19:10:24: 8000000 INFO @ Sat, 11 Dec 2021 19:10:28: 5000000 INFO @ Sat, 11 Dec 2021 19:10:33: 9000000 INFO @ Sat, 11 Dec 2021 19:10:33: 11000000 INFO @ Sat, 11 Dec 2021 19:10:37: 6000000 INFO @ Sat, 11 Dec 2021 19:10:41: 10000000 INFO @ Sat, 11 Dec 2021 19:10:42: 12000000 INFO @ Sat, 11 Dec 2021 19:10:46: 7000000 INFO @ Sat, 11 Dec 2021 19:10:50: 11000000 INFO @ Sat, 11 Dec 2021 19:10:52: 13000000 INFO @ Sat, 11 Dec 2021 19:10:55: 8000000 INFO @ Sat, 11 Dec 2021 19:10:59: 12000000 INFO @ Sat, 11 Dec 2021 19:11:02: 14000000 INFO @ Sat, 11 Dec 2021 19:11:04: 9000000 INFO @ Sat, 11 Dec 2021 19:11:07: 13000000 INFO @ Sat, 11 Dec 2021 19:11:11: 15000000 INFO @ Sat, 11 Dec 2021 19:11:12: 10000000 INFO @ Sat, 11 Dec 2021 19:11:16: 14000000 INFO @ Sat, 11 Dec 2021 19:11:21: 16000000 INFO @ Sat, 11 Dec 2021 19:11:21: 11000000 INFO @ Sat, 11 Dec 2021 19:11:25: 15000000 INFO @ Sat, 11 Dec 2021 19:11:30: 17000000 INFO @ Sat, 11 Dec 2021 19:11:30: 12000000 INFO @ Sat, 11 Dec 2021 19:11:33: 16000000 INFO @ Sat, 11 Dec 2021 19:11:39: 13000000 INFO @ Sat, 11 Dec 2021 19:11:39: 18000000 INFO @ Sat, 11 Dec 2021 19:11:42: 17000000 INFO @ Sat, 11 Dec 2021 19:11:47: 14000000 INFO @ Sat, 11 Dec 2021 19:11:49: 19000000 INFO @ Sat, 11 Dec 2021 19:11:50: 18000000 INFO @ Sat, 11 Dec 2021 19:11:56: 15000000 INFO @ Sat, 11 Dec 2021 19:11:58: 19000000 INFO @ Sat, 11 Dec 2021 19:11:58: 20000000 INFO @ Sat, 11 Dec 2021 19:12:05: 16000000 INFO @ Sat, 11 Dec 2021 19:12:07: 20000000 INFO @ Sat, 11 Dec 2021 19:12:08: 21000000 INFO @ Sat, 11 Dec 2021 19:12:13: 17000000 INFO @ Sat, 11 Dec 2021 19:12:16: 21000000 INFO @ Sat, 11 Dec 2021 19:12:18: 22000000 INFO @ Sat, 11 Dec 2021 19:12:22: 18000000 INFO @ Sat, 11 Dec 2021 19:12:24: 22000000 INFO @ Sat, 11 Dec 2021 19:12:27: 23000000 INFO @ Sat, 11 Dec 2021 19:12:30: 19000000 INFO @ Sat, 11 Dec 2021 19:12:33: 23000000 INFO @ Sat, 11 Dec 2021 19:12:37: 24000000 INFO @ Sat, 11 Dec 2021 19:12:39: 20000000 INFO @ Sat, 11 Dec 2021 19:12:42: 24000000 INFO @ Sat, 11 Dec 2021 19:12:47: 25000000 INFO @ Sat, 11 Dec 2021 19:12:47: 21000000 INFO @ Sat, 11 Dec 2021 19:12:50: 25000000 INFO @ Sat, 11 Dec 2021 19:12:56: 22000000 INFO @ Sat, 11 Dec 2021 19:12:57: 26000000 INFO @ Sat, 11 Dec 2021 19:12:59: 26000000 INFO @ Sat, 11 Dec 2021 19:13:05: 23000000 INFO @ Sat, 11 Dec 2021 19:13:06: 27000000 INFO @ Sat, 11 Dec 2021 19:13:08: 27000000 INFO @ Sat, 11 Dec 2021 19:13:14: 24000000 INFO @ Sat, 11 Dec 2021 19:13:16: 28000000 INFO @ Sat, 11 Dec 2021 19:13:16: 28000000 INFO @ Sat, 11 Dec 2021 19:13:23: 25000000 INFO @ Sat, 11 Dec 2021 19:13:26: 29000000 INFO @ Sat, 11 Dec 2021 19:13:26: 29000000 INFO @ Sat, 11 Dec 2021 19:13:32: 26000000 INFO @ Sat, 11 Dec 2021 19:13:34: 30000000 INFO @ Sat, 11 Dec 2021 19:13:35: 30000000 INFO @ Sat, 11 Dec 2021 19:13:41: 27000000 INFO @ Sat, 11 Dec 2021 19:13:43: 31000000 INFO @ Sat, 11 Dec 2021 19:13:45: 31000000 INFO @ Sat, 11 Dec 2021 19:13:49: 28000000 INFO @ Sat, 11 Dec 2021 19:13:52: 32000000 INFO @ Sat, 11 Dec 2021 19:13:56: 32000000 INFO @ Sat, 11 Dec 2021 19:13:58: 29000000 INFO @ Sat, 11 Dec 2021 19:14:01: 33000000 INFO @ Sat, 11 Dec 2021 19:14:05: 33000000 INFO @ Sat, 11 Dec 2021 19:14:07: 30000000 INFO @ Sat, 11 Dec 2021 19:14:09: 34000000 INFO @ Sat, 11 Dec 2021 19:14:15: 34000000 INFO @ Sat, 11 Dec 2021 19:14:16: 31000000 INFO @ Sat, 11 Dec 2021 19:14:18: 35000000 INFO @ Sat, 11 Dec 2021 19:14:24: 32000000 INFO @ Sat, 11 Dec 2021 19:14:25: 35000000 INFO @ Sat, 11 Dec 2021 19:14:27: 36000000 INFO @ Sat, 11 Dec 2021 19:14:33: 33000000 INFO @ Sat, 11 Dec 2021 19:14:35: 36000000 INFO @ Sat, 11 Dec 2021 19:14:35: 37000000 INFO @ Sat, 11 Dec 2021 19:14:41: 34000000 INFO @ Sat, 11 Dec 2021 19:14:44: 38000000 INFO @ Sat, 11 Dec 2021 19:14:44: 37000000 INFO @ Sat, 11 Dec 2021 19:14:50: 35000000 INFO @ Sat, 11 Dec 2021 19:14:52: 39000000 INFO @ Sat, 11 Dec 2021 19:14:54: 38000000 INFO @ Sat, 11 Dec 2021 19:14:59: 36000000 INFO @ Sat, 11 Dec 2021 19:15:01: 40000000 INFO @ Sat, 11 Dec 2021 19:15:03: 39000000 INFO @ Sat, 11 Dec 2021 19:15:08: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 19:15:10: 41000000 INFO @ Sat, 11 Dec 2021 19:15:13: 40000000 INFO @ Sat, 11 Dec 2021 19:15:16: 38000000 INFO @ Sat, 11 Dec 2021 19:15:18: 42000000 INFO @ Sat, 11 Dec 2021 19:15:22: 41000000 INFO @ Sat, 11 Dec 2021 19:15:25: 39000000 INFO @ Sat, 11 Dec 2021 19:15:25: 43000000 INFO @ Sat, 11 Dec 2021 19:15:32: 42000000 INFO @ Sat, 11 Dec 2021 19:15:33: 44000000 INFO @ Sat, 11 Dec 2021 19:15:34: 40000000 INFO @ Sat, 11 Dec 2021 19:15:40: 43000000 INFO @ Sat, 11 Dec 2021 19:15:40: 45000000 INFO @ Sat, 11 Dec 2021 19:15:43: 41000000 INFO @ Sat, 11 Dec 2021 19:15:48: 46000000 INFO @ Sat, 11 Dec 2021 19:15:48: 44000000 INFO @ Sat, 11 Dec 2021 19:15:51: 42000000 INFO @ Sat, 11 Dec 2021 19:15:56: 47000000 INFO @ Sat, 11 Dec 2021 19:15:57: 45000000 INFO @ Sat, 11 Dec 2021 19:15:59: 43000000 INFO @ Sat, 11 Dec 2021 19:16:03: 48000000 INFO @ Sat, 11 Dec 2021 19:16:05: 46000000 INFO @ Sat, 11 Dec 2021 19:16:06: 44000000 INFO @ Sat, 11 Dec 2021 19:16:11: 49000000 INFO @ Sat, 11 Dec 2021 19:16:13: 47000000 INFO @ Sat, 11 Dec 2021 19:16:14: 45000000 INFO @ Sat, 11 Dec 2021 19:16:20: 50000000 INFO @ Sat, 11 Dec 2021 19:16:21: 46000000 INFO @ Sat, 11 Dec 2021 19:16:21: 48000000 INFO @ Sat, 11 Dec 2021 19:16:29: 47000000 INFO @ Sat, 11 Dec 2021 19:16:29: 51000000 INFO @ Sat, 11 Dec 2021 19:16:30: 49000000 INFO @ Sat, 11 Dec 2021 19:16:36: 48000000 INFO @ Sat, 11 Dec 2021 19:16:38: 52000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 19:16:40: 50000000 INFO @ Sat, 11 Dec 2021 19:16:44: 49000000 INFO @ Sat, 11 Dec 2021 19:16:47: 53000000 INFO @ Sat, 11 Dec 2021 19:16:50: 51000000 INFO @ Sat, 11 Dec 2021 19:16:54: 50000000 INFO @ Sat, 11 Dec 2021 19:16:56: 54000000 INFO @ Sat, 11 Dec 2021 19:16:59: 52000000 INFO @ Sat, 11 Dec 2021 19:17:02: 51000000 INFO @ Sat, 11 Dec 2021 19:17:05: 55000000 INFO @ Sat, 11 Dec 2021 19:17:09: 53000000 INFO @ Sat, 11 Dec 2021 19:17:11: 52000000 INFO @ Sat, 11 Dec 2021 19:17:14: 56000000 INFO @ Sat, 11 Dec 2021 19:17:19: 54000000 INFO @ Sat, 11 Dec 2021 19:17:20: 53000000 INFO @ Sat, 11 Dec 2021 19:17:22: 57000000 INFO @ Sat, 11 Dec 2021 19:17:29: 54000000 INFO @ Sat, 11 Dec 2021 19:17:30: 58000000 INFO @ Sat, 11 Dec 2021 19:17:30: 55000000 INFO @ Sat, 11 Dec 2021 19:17:38: 59000000 INFO @ Sat, 11 Dec 2021 19:17:38: 55000000 INFO @ Sat, 11 Dec 2021 19:17:40: 56000000 INFO @ Sat, 11 Dec 2021 19:17:45: 60000000 INFO @ Sat, 11 Dec 2021 19:17:47: 56000000 INFO @ Sat, 11 Dec 2021 19:17:49: 57000000 INFO @ Sat, 11 Dec 2021 19:17:53: 61000000 INFO @ Sat, 11 Dec 2021 19:17:55: 57000000 INFO @ Sat, 11 Dec 2021 19:17:57: 58000000 INFO @ Sat, 11 Dec 2021 19:18:01: 62000000 INFO @ Sat, 11 Dec 2021 19:18:03: 58000000 INFO @ Sat, 11 Dec 2021 19:18:06: 59000000 INFO @ Sat, 11 Dec 2021 19:18:09: 63000000 INFO @ Sat, 11 Dec 2021 19:18:10: 59000000 INFO @ Sat, 11 Dec 2021 19:18:14: 60000000 INFO @ Sat, 11 Dec 2021 19:18:17: 64000000 INFO @ Sat, 11 Dec 2021 19:18:18: 60000000 INFO @ Sat, 11 Dec 2021 19:18:23: 61000000 INFO @ Sat, 11 Dec 2021 19:18:26: 61000000 INFO @ Sat, 11 Dec 2021 19:18:26: 65000000 INFO @ Sat, 11 Dec 2021 19:18:31: 62000000 INFO @ Sat, 11 Dec 2021 19:18:34: 62000000 INFO @ Sat, 11 Dec 2021 19:18:35: 66000000 INFO @ Sat, 11 Dec 2021 19:18:40: 63000000 INFO @ Sat, 11 Dec 2021 19:18:41: 63000000 INFO @ Sat, 11 Dec 2021 19:18:44: 67000000 INFO @ Sat, 11 Dec 2021 19:18:49: 64000000 INFO @ Sat, 11 Dec 2021 19:18:50: 64000000 INFO @ Sat, 11 Dec 2021 19:18:53: 68000000 INFO @ Sat, 11 Dec 2021 19:18:58: 65000000 INFO @ Sat, 11 Dec 2021 19:18:59: 65000000 INFO @ Sat, 11 Dec 2021 19:19:02: 69000000 INFO @ Sat, 11 Dec 2021 19:19:08: 66000000 INFO @ Sat, 11 Dec 2021 19:19:08: 66000000 INFO @ Sat, 11 Dec 2021 19:19:10: 70000000 INFO @ Sat, 11 Dec 2021 19:19:17: 67000000 INFO @ Sat, 11 Dec 2021 19:19:18: 67000000 INFO @ Sat, 11 Dec 2021 19:19:18: #1 tag size is determined as 79 bps INFO @ Sat, 11 Dec 2021 19:19:18: #1 tag size = 79 INFO @ Sat, 11 Dec 2021 19:19:18: #1 total tags in treatment: 21889292 INFO @ Sat, 11 Dec 2021 19:19:18: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:19:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:19:18: #1 tags after filtering in treatment: 14312648 INFO @ Sat, 11 Dec 2021 19:19:18: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 11 Dec 2021 19:19:18: #1 finished! INFO @ Sat, 11 Dec 2021 19:19:18: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:19:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:19:19: #2 number of paired peaks: 710 WARNING @ Sat, 11 Dec 2021 19:19:19: Fewer paired peaks (710) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 710 pairs to build model! INFO @ Sat, 11 Dec 2021 19:19:19: start model_add_line... INFO @ Sat, 11 Dec 2021 19:19:19: start X-correlation... INFO @ Sat, 11 Dec 2021 19:19:20: end of X-cor INFO @ Sat, 11 Dec 2021 19:19:20: #2 finished! INFO @ Sat, 11 Dec 2021 19:19:20: #2 predicted fragment length is 89 bps INFO @ Sat, 11 Dec 2021 19:19:20: #2 alternative fragment length(s) may be 89 bps INFO @ Sat, 11 Dec 2021 19:19:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.10_model.r WARNING @ Sat, 11 Dec 2021 19:19:20: #2 Since the d (89) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:19:20: #2 You may need to consider one of the other alternative d(s): 89 WARNING @ Sat, 11 Dec 2021 19:19:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:19:20: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:19:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:19:26: 68000000 INFO @ Sat, 11 Dec 2021 19:19:27: 68000000 INFO @ Sat, 11 Dec 2021 19:19:34: 69000000 INFO @ Sat, 11 Dec 2021 19:19:37: 69000000 INFO @ Sat, 11 Dec 2021 19:19:43: 70000000 INFO @ Sat, 11 Dec 2021 19:19:47: 70000000 INFO @ Sat, 11 Dec 2021 19:19:48: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:19:50: #1 tag size is determined as 79 bps INFO @ Sat, 11 Dec 2021 19:19:50: #1 tag size = 79 INFO @ Sat, 11 Dec 2021 19:19:50: #1 total tags in treatment: 21889292 INFO @ Sat, 11 Dec 2021 19:19:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:19:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:19:51: #1 tags after filtering in treatment: 14312648 INFO @ Sat, 11 Dec 2021 19:19:51: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 11 Dec 2021 19:19:51: #1 finished! INFO @ Sat, 11 Dec 2021 19:19:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:19:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:19:52: #2 number of paired peaks: 710 WARNING @ Sat, 11 Dec 2021 19:19:52: Fewer paired peaks (710) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 710 pairs to build model! INFO @ Sat, 11 Dec 2021 19:19:52: start model_add_line... INFO @ Sat, 11 Dec 2021 19:19:52: start X-correlation... INFO @ Sat, 11 Dec 2021 19:19:52: end of X-cor INFO @ Sat, 11 Dec 2021 19:19:52: #2 finished! INFO @ Sat, 11 Dec 2021 19:19:52: #2 predicted fragment length is 89 bps INFO @ Sat, 11 Dec 2021 19:19:52: #2 alternative fragment length(s) may be 89 bps INFO @ Sat, 11 Dec 2021 19:19:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.20_model.r WARNING @ Sat, 11 Dec 2021 19:19:52: #2 Since the d (89) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:19:52: #2 You may need to consider one of the other alternative d(s): 89 WARNING @ Sat, 11 Dec 2021 19:19:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:19:52: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:19:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:19:55: #1 tag size is determined as 79 bps INFO @ Sat, 11 Dec 2021 19:19:55: #1 tag size = 79 INFO @ Sat, 11 Dec 2021 19:19:55: #1 total tags in treatment: 21889292 INFO @ Sat, 11 Dec 2021 19:19:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 19:19:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 19:19:55: #1 tags after filtering in treatment: 14312648 INFO @ Sat, 11 Dec 2021 19:19:55: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 11 Dec 2021 19:19:55: #1 finished! INFO @ Sat, 11 Dec 2021 19:19:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 19:19:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 19:19:56: #2 number of paired peaks: 710 WARNING @ Sat, 11 Dec 2021 19:19:56: Fewer paired peaks (710) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 710 pairs to build model! INFO @ Sat, 11 Dec 2021 19:19:56: start model_add_line... INFO @ Sat, 11 Dec 2021 19:19:57: start X-correlation... INFO @ Sat, 11 Dec 2021 19:19:57: end of X-cor INFO @ Sat, 11 Dec 2021 19:19:57: #2 finished! INFO @ Sat, 11 Dec 2021 19:19:57: #2 predicted fragment length is 89 bps INFO @ Sat, 11 Dec 2021 19:19:57: #2 alternative fragment length(s) may be 89 bps INFO @ Sat, 11 Dec 2021 19:19:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.05_model.r WARNING @ Sat, 11 Dec 2021 19:19:57: #2 Since the d (89) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 19:19:57: #2 You may need to consider one of the other alternative d(s): 89 WARNING @ Sat, 11 Dec 2021 19:19:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 19:19:57: #3 Call peaks... INFO @ Sat, 11 Dec 2021 19:19:57: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 19:20:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.10_peaks.xls INFO @ Sat, 11 Dec 2021 19:20:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:20:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.10_summits.bed INFO @ Sat, 11 Dec 2021 19:20:05: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (9050 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 19:20:20: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:20:25: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 19:20:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.20_peaks.xls INFO @ Sat, 11 Dec 2021 19:20:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:20:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.20_summits.bed INFO @ Sat, 11 Dec 2021 19:20:37: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (3281 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 19:20:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.05_peaks.xls INFO @ Sat, 11 Dec 2021 19:20:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 19:20:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9689641/SRX9689641.05_summits.bed INFO @ Sat, 11 Dec 2021 19:20:43: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (15960 records, 4 fields): 21 millis CompletedMACS2peakCalling