Job ID = 14171230 SRX = SRX9555336 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 1935626 spots for SRR13110849/SRR13110849.sra Written 1935626 spots for SRR13110849/SRR13110849.sra Read 1788182 spots for SRR13110850/SRR13110850.sra Written 1788182 spots for SRR13110850/SRR13110850.sra Read 1803353 spots for SRR13110851/SRR13110851.sra Written 1803353 spots for SRR13110851/SRR13110851.sra Read 1704125 spots for SRR13110852/SRR13110852.sra Written 1704125 spots for SRR13110852/SRR13110852.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171682 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:35 7231286 reads; of these: 7231286 (100.00%) were paired; of these: 7050508 (97.50%) aligned concordantly 0 times 123672 (1.71%) aligned concordantly exactly 1 time 57106 (0.79%) aligned concordantly >1 times ---- 7050508 pairs aligned concordantly 0 times; of these: 1324 (0.02%) aligned discordantly 1 time ---- 7049184 pairs aligned 0 times concordantly or discordantly; of these: 14098368 mates make up the pairs; of these: 14074959 (99.83%) aligned 0 times 5723 (0.04%) aligned exactly 1 time 17686 (0.13%) aligned >1 times 2.68% overall alignment rate Time searching: 00:01:35 Overall time: 00:01:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1206 / 55372 = 0.0218 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:40:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:40:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:40:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:40:29: #1 tag size is determined as 45 bps INFO @ Sat, 11 Dec 2021 10:40:29: #1 tag size = 45 INFO @ Sat, 11 Dec 2021 10:40:29: #1 total tags in treatment: 179575 INFO @ Sat, 11 Dec 2021 10:40:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:40:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:40:29: #1 tags after filtering in treatment: 174596 INFO @ Sat, 11 Dec 2021 10:40:29: #1 Redundant rate of treatment: 0.03 INFO @ Sat, 11 Dec 2021 10:40:29: #1 finished! INFO @ Sat, 11 Dec 2021 10:40:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:40:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:40:29: #2 number of paired peaks: 2239 INFO @ Sat, 11 Dec 2021 10:40:29: start model_add_line... INFO @ Sat, 11 Dec 2021 10:40:29: start X-correlation... INFO @ Sat, 11 Dec 2021 10:40:29: end of X-cor INFO @ Sat, 11 Dec 2021 10:40:29: #2 finished! INFO @ Sat, 11 Dec 2021 10:40:29: #2 predicted fragment length is 48 bps INFO @ Sat, 11 Dec 2021 10:40:29: #2 alternative fragment length(s) may be 48,97,162,247,561 bps INFO @ Sat, 11 Dec 2021 10:40:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.05_model.r WARNING @ Sat, 11 Dec 2021 10:40:29: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 10:40:29: #2 You may need to consider one of the other alternative d(s): 48,97,162,247,561 WARNING @ Sat, 11 Dec 2021 10:40:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 10:40:29: #3 Call peaks... INFO @ Sat, 11 Dec 2021 10:40:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 10:40:30: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 10:40:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.05_peaks.xls INFO @ Sat, 11 Dec 2021 10:40:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 10:40:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.05_summits.bed INFO @ Sat, 11 Dec 2021 10:40:30: Done! pass1 - making usageList (8 chroms): 0 millis pass2 - checking and writing primary data (138 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:40:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:40:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:40:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 10:40:59: #1 tag size is determined as 45 bps INFO @ Sat, 11 Dec 2021 10:40:59: #1 tag size = 45 INFO @ Sat, 11 Dec 2021 10:40:59: #1 total tags in treatment: 179575 INFO @ Sat, 11 Dec 2021 10:40:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:40:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:40:59: #1 tags after filtering in treatment: 174596 INFO @ Sat, 11 Dec 2021 10:40:59: #1 Redundant rate of treatment: 0.03 INFO @ Sat, 11 Dec 2021 10:40:59: #1 finished! INFO @ Sat, 11 Dec 2021 10:40:59: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:40:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:40:59: #2 number of paired peaks: 2239 INFO @ Sat, 11 Dec 2021 10:40:59: start model_add_line... INFO @ Sat, 11 Dec 2021 10:40:59: start X-correlation... INFO @ Sat, 11 Dec 2021 10:40:59: end of X-cor INFO @ Sat, 11 Dec 2021 10:40:59: #2 finished! INFO @ Sat, 11 Dec 2021 10:40:59: #2 predicted fragment length is 48 bps INFO @ Sat, 11 Dec 2021 10:40:59: #2 alternative fragment length(s) may be 48,97,162,247,561 bps INFO @ Sat, 11 Dec 2021 10:40:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.10_model.r WARNING @ Sat, 11 Dec 2021 10:40:59: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 10:40:59: #2 You may need to consider one of the other alternative d(s): 48,97,162,247,561 WARNING @ Sat, 11 Dec 2021 10:40:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 10:40:59: #3 Call peaks... INFO @ Sat, 11 Dec 2021 10:40:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 10:41:00: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 10:41:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.10_peaks.xls INFO @ Sat, 11 Dec 2021 10:41:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 10:41:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.10_summits.bed INFO @ Sat, 11 Dec 2021 10:41:00: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (59 records, 4 fields): 15 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 10:41:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 10:41:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 10:41:27: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 10:41:29: #1 tag size is determined as 45 bps INFO @ Sat, 11 Dec 2021 10:41:29: #1 tag size = 45 INFO @ Sat, 11 Dec 2021 10:41:29: #1 total tags in treatment: 179575 INFO @ Sat, 11 Dec 2021 10:41:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:41:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:41:29: #1 tags after filtering in treatment: 174596 INFO @ Sat, 11 Dec 2021 10:41:29: #1 Redundant rate of treatment: 0.03 INFO @ Sat, 11 Dec 2021 10:41:29: #1 finished! INFO @ Sat, 11 Dec 2021 10:41:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:41:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:41:29: #2 number of paired peaks: 2239 INFO @ Sat, 11 Dec 2021 10:41:29: start model_add_line... INFO @ Sat, 11 Dec 2021 10:41:29: start X-correlation... INFO @ Sat, 11 Dec 2021 10:41:29: end of X-cor INFO @ Sat, 11 Dec 2021 10:41:29: #2 finished! INFO @ Sat, 11 Dec 2021 10:41:29: #2 predicted fragment length is 48 bps INFO @ Sat, 11 Dec 2021 10:41:29: #2 alternative fragment length(s) may be 48,97,162,247,561 bps INFO @ Sat, 11 Dec 2021 10:41:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.20_model.r WARNING @ Sat, 11 Dec 2021 10:41:29: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 10:41:29: #2 You may need to consider one of the other alternative d(s): 48,97,162,247,561 WARNING @ Sat, 11 Dec 2021 10:41:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 10:41:29: #3 Call peaks... INFO @ Sat, 11 Dec 2021 10:41:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 10:41:30: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 10:41:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.20_peaks.xls INFO @ Sat, 11 Dec 2021 10:41:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 10:41:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9555336/SRX9555336.20_summits.bed INFO @ Sat, 11 Dec 2021 10:41:30: Done! pass1 - making usageList (5 chroms): 0 millis pass2 - checking and writing primary data (28 records, 4 fields): 18 millis CompletedMACS2peakCalling