Job ID = 16439608 SRX = SRX9518341 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26942224 spots for SRR13070824/SRR13070824.sra Written 26942224 spots for SRR13070824/SRR13070824.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440048 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:00:16 26942224 reads; of these: 26942224 (100.00%) were paired; of these: 2191494 (8.13%) aligned concordantly 0 times 17951041 (66.63%) aligned concordantly exactly 1 time 6799689 (25.24%) aligned concordantly >1 times ---- 2191494 pairs aligned concordantly 0 times; of these: 381655 (17.42%) aligned discordantly 1 time ---- 1809839 pairs aligned 0 times concordantly or discordantly; of these: 3619678 mates make up the pairs; of these: 2011286 (55.57%) aligned 0 times 837506 (23.14%) aligned exactly 1 time 770886 (21.30%) aligned >1 times 96.27% overall alignment rate Time searching: 01:00:16 Overall time: 01:00:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1319043 / 24897240 = 0.0530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:56:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:56:31: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:56:31: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:56:37: 1000000 INFO @ Tue, 02 Aug 2022 16:56:44: 2000000 INFO @ Tue, 02 Aug 2022 16:56:50: 3000000 INFO @ Tue, 02 Aug 2022 16:56:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:57:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:57:01: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:57:01: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:57:03: 5000000 INFO @ Tue, 02 Aug 2022 16:57:08: 1000000 INFO @ Tue, 02 Aug 2022 16:57:10: 6000000 INFO @ Tue, 02 Aug 2022 16:57:16: 2000000 INFO @ Tue, 02 Aug 2022 16:57:17: 7000000 INFO @ Tue, 02 Aug 2022 16:57:23: 3000000 INFO @ Tue, 02 Aug 2022 16:57:25: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:57:30: 4000000 INFO @ Tue, 02 Aug 2022 16:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:57:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:57:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:57:33: 9000000 INFO @ Tue, 02 Aug 2022 16:57:39: 5000000 INFO @ Tue, 02 Aug 2022 16:57:41: 1000000 INFO @ Tue, 02 Aug 2022 16:57:41: 10000000 INFO @ Tue, 02 Aug 2022 16:57:47: 6000000 INFO @ Tue, 02 Aug 2022 16:57:50: 11000000 INFO @ Tue, 02 Aug 2022 16:57:50: 2000000 INFO @ Tue, 02 Aug 2022 16:57:55: 7000000 INFO @ Tue, 02 Aug 2022 16:57:58: 12000000 INFO @ Tue, 02 Aug 2022 16:57:59: 3000000 INFO @ Tue, 02 Aug 2022 16:58:04: 8000000 INFO @ Tue, 02 Aug 2022 16:58:07: 13000000 INFO @ Tue, 02 Aug 2022 16:58:08: 4000000 INFO @ Tue, 02 Aug 2022 16:58:12: 9000000 INFO @ Tue, 02 Aug 2022 16:58:15: 14000000 INFO @ Tue, 02 Aug 2022 16:58:17: 5000000 INFO @ Tue, 02 Aug 2022 16:58:21: 10000000 INFO @ Tue, 02 Aug 2022 16:58:23: 15000000 INFO @ Tue, 02 Aug 2022 16:58:26: 6000000 INFO @ Tue, 02 Aug 2022 16:58:30: 11000000 INFO @ Tue, 02 Aug 2022 16:58:32: 16000000 INFO @ Tue, 02 Aug 2022 16:58:35: 7000000 INFO @ Tue, 02 Aug 2022 16:58:38: 12000000 INFO @ Tue, 02 Aug 2022 16:58:40: 17000000 INFO @ Tue, 02 Aug 2022 16:58:44: 8000000 INFO @ Tue, 02 Aug 2022 16:58:47: 13000000 INFO @ Tue, 02 Aug 2022 16:58:49: 18000000 INFO @ Tue, 02 Aug 2022 16:58:52: 9000000 INFO @ Tue, 02 Aug 2022 16:58:55: 14000000 INFO @ Tue, 02 Aug 2022 16:58:58: 19000000 INFO @ Tue, 02 Aug 2022 16:59:01: 10000000 INFO @ Tue, 02 Aug 2022 16:59:04: 15000000 INFO @ Tue, 02 Aug 2022 16:59:06: 20000000 INFO @ Tue, 02 Aug 2022 16:59:09: 11000000 INFO @ Tue, 02 Aug 2022 16:59:12: 16000000 INFO @ Tue, 02 Aug 2022 16:59:15: 21000000 INFO @ Tue, 02 Aug 2022 16:59:18: 12000000 INFO @ Tue, 02 Aug 2022 16:59:21: 17000000 INFO @ Tue, 02 Aug 2022 16:59:23: 22000000 INFO @ Tue, 02 Aug 2022 16:59:26: 13000000 INFO @ Tue, 02 Aug 2022 16:59:29: 18000000 INFO @ Tue, 02 Aug 2022 16:59:31: 23000000 INFO @ Tue, 02 Aug 2022 16:59:35: 14000000 INFO @ Tue, 02 Aug 2022 16:59:38: 19000000 INFO @ Tue, 02 Aug 2022 16:59:40: 24000000 INFO @ Tue, 02 Aug 2022 16:59:44: 15000000 INFO @ Tue, 02 Aug 2022 16:59:46: 20000000 INFO @ Tue, 02 Aug 2022 16:59:48: 25000000 INFO @ Tue, 02 Aug 2022 16:59:52: 16000000 INFO @ Tue, 02 Aug 2022 16:59:55: 21000000 INFO @ Tue, 02 Aug 2022 16:59:57: 26000000 INFO @ Tue, 02 Aug 2022 17:00:01: 17000000 INFO @ Tue, 02 Aug 2022 17:00:03: 22000000 INFO @ Tue, 02 Aug 2022 17:00:05: 27000000 INFO @ Tue, 02 Aug 2022 17:00:09: 18000000 INFO @ Tue, 02 Aug 2022 17:00:11: 23000000 INFO @ Tue, 02 Aug 2022 17:00:14: 28000000 INFO @ Tue, 02 Aug 2022 17:00:18: 19000000 INFO @ Tue, 02 Aug 2022 17:00:19: 24000000 INFO @ Tue, 02 Aug 2022 17:00:22: 29000000 INFO @ Tue, 02 Aug 2022 17:00:27: 20000000 INFO @ Tue, 02 Aug 2022 17:00:28: 25000000 INFO @ Tue, 02 Aug 2022 17:00:30: 30000000 INFO @ Tue, 02 Aug 2022 17:00:35: 21000000 INFO @ Tue, 02 Aug 2022 17:00:36: 26000000 INFO @ Tue, 02 Aug 2022 17:00:39: 31000000 INFO @ Tue, 02 Aug 2022 17:00:43: 22000000 INFO @ Tue, 02 Aug 2022 17:00:44: 27000000 INFO @ Tue, 02 Aug 2022 17:00:47: 32000000 INFO @ Tue, 02 Aug 2022 17:00:52: 23000000 INFO @ Tue, 02 Aug 2022 17:00:53: 28000000 INFO @ Tue, 02 Aug 2022 17:00:56: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:01:00: 24000000 INFO @ Tue, 02 Aug 2022 17:01:01: 29000000 INFO @ Tue, 02 Aug 2022 17:01:04: 34000000 INFO @ Tue, 02 Aug 2022 17:01:09: 25000000 INFO @ Tue, 02 Aug 2022 17:01:09: 30000000 INFO @ Tue, 02 Aug 2022 17:01:13: 35000000 INFO @ Tue, 02 Aug 2022 17:01:17: 26000000 INFO @ Tue, 02 Aug 2022 17:01:17: 31000000 INFO @ Tue, 02 Aug 2022 17:01:21: 36000000 INFO @ Tue, 02 Aug 2022 17:01:25: 27000000 INFO @ Tue, 02 Aug 2022 17:01:26: 32000000 INFO @ Tue, 02 Aug 2022 17:01:30: 37000000 INFO @ Tue, 02 Aug 2022 17:01:34: 28000000 INFO @ Tue, 02 Aug 2022 17:01:34: 33000000 INFO @ Tue, 02 Aug 2022 17:01:38: 38000000 INFO @ Tue, 02 Aug 2022 17:01:42: 29000000 INFO @ Tue, 02 Aug 2022 17:01:42: 34000000 INFO @ Tue, 02 Aug 2022 17:01:47: 39000000 INFO @ Tue, 02 Aug 2022 17:01:51: 30000000 INFO @ Tue, 02 Aug 2022 17:01:51: 35000000 INFO @ Tue, 02 Aug 2022 17:01:55: 40000000 INFO @ Tue, 02 Aug 2022 17:01:59: 31000000 INFO @ Tue, 02 Aug 2022 17:01:59: 36000000 INFO @ Tue, 02 Aug 2022 17:02:03: 41000000 INFO @ Tue, 02 Aug 2022 17:02:07: 32000000 INFO @ Tue, 02 Aug 2022 17:02:08: 37000000 INFO @ Tue, 02 Aug 2022 17:02:12: 42000000 INFO @ Tue, 02 Aug 2022 17:02:16: 33000000 INFO @ Tue, 02 Aug 2022 17:02:16: 38000000 INFO @ Tue, 02 Aug 2022 17:02:20: 43000000 INFO @ Tue, 02 Aug 2022 17:02:24: 34000000 INFO @ Tue, 02 Aug 2022 17:02:24: 39000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:02:29: 44000000 INFO @ Tue, 02 Aug 2022 17:02:33: 35000000 INFO @ Tue, 02 Aug 2022 17:02:33: 40000000 INFO @ Tue, 02 Aug 2022 17:02:37: 45000000 INFO @ Tue, 02 Aug 2022 17:02:41: 41000000 INFO @ Tue, 02 Aug 2022 17:02:41: 36000000 INFO @ Tue, 02 Aug 2022 17:02:45: 46000000 INFO @ Tue, 02 Aug 2022 17:02:50: 42000000 INFO @ Tue, 02 Aug 2022 17:02:50: 37000000 INFO @ Tue, 02 Aug 2022 17:02:54: 47000000 INFO @ Tue, 02 Aug 2022 17:02:58: 43000000 INFO @ Tue, 02 Aug 2022 17:02:59: 38000000 INFO @ Tue, 02 Aug 2022 17:03:02: 48000000 INFO @ Tue, 02 Aug 2022 17:03:07: 44000000 INFO @ Tue, 02 Aug 2022 17:03:08: 39000000 INFO @ Tue, 02 Aug 2022 17:03:10: 49000000 INFO @ Tue, 02 Aug 2022 17:03:12: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 17:03:12: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 17:03:12: #1 total tags in treatment: 23436294 INFO @ Tue, 02 Aug 2022 17:03:12: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:03:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:03:13: #1 tags after filtering in treatment: 21789441 INFO @ Tue, 02 Aug 2022 17:03:13: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 17:03:13: #1 finished! INFO @ Tue, 02 Aug 2022 17:03:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:03:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:03:14: #2 number of paired peaks: 67 WARNING @ Tue, 02 Aug 2022 17:03:14: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:03:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:03:15: 45000000 INFO @ Tue, 02 Aug 2022 17:03:16: 40000000 INFO @ Tue, 02 Aug 2022 17:03:23: 46000000 INFO @ Tue, 02 Aug 2022 17:03:25: 41000000 INFO @ Tue, 02 Aug 2022 17:03:31: 47000000 INFO @ Tue, 02 Aug 2022 17:03:34: 42000000 INFO @ Tue, 02 Aug 2022 17:03:39: 48000000 INFO @ Tue, 02 Aug 2022 17:03:43: 43000000 INFO @ Tue, 02 Aug 2022 17:03:47: 49000000 INFO @ Tue, 02 Aug 2022 17:03:49: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 17:03:49: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 17:03:49: #1 total tags in treatment: 23436294 INFO @ Tue, 02 Aug 2022 17:03:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:03:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:03:49: #1 tags after filtering in treatment: 21789441 INFO @ Tue, 02 Aug 2022 17:03:49: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 17:03:49: #1 finished! INFO @ Tue, 02 Aug 2022 17:03:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:03:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:03:51: #2 number of paired peaks: 67 WARNING @ Tue, 02 Aug 2022 17:03:51: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:03:51: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 17:03:52: 44000000 cut: /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:03:59: 45000000 INFO @ Tue, 02 Aug 2022 17:04:06: 46000000 INFO @ Tue, 02 Aug 2022 17:04:13: 47000000 INFO @ Tue, 02 Aug 2022 17:04:20: 48000000 INFO @ Tue, 02 Aug 2022 17:04:27: 49000000 INFO @ Tue, 02 Aug 2022 17:04:29: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 17:04:29: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 17:04:29: #1 total tags in treatment: 23436294 INFO @ Tue, 02 Aug 2022 17:04:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:04:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:04:29: #1 tags after filtering in treatment: 21789441 INFO @ Tue, 02 Aug 2022 17:04:29: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 17:04:29: #1 finished! INFO @ Tue, 02 Aug 2022 17:04:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:04:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:04:30: #2 number of paired peaks: 67 WARNING @ Tue, 02 Aug 2022 17:04:30: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:04:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518341/SRX9518341.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling