Job ID = 16439462 SRX = SRX9518320 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20251427 spots for SRR13070803/SRR13070803.sra Written 20251427 spots for SRR13070803/SRR13070803.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439865 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:02:54 20251427 reads; of these: 20251427 (100.00%) were paired; of these: 1522913 (7.52%) aligned concordantly 0 times 14091068 (69.58%) aligned concordantly exactly 1 time 4637446 (22.90%) aligned concordantly >1 times ---- 1522913 pairs aligned concordantly 0 times; of these: 218051 (14.32%) aligned discordantly 1 time ---- 1304862 pairs aligned 0 times concordantly or discordantly; of these: 2609724 mates make up the pairs; of these: 1757648 (67.35%) aligned 0 times 395695 (15.16%) aligned exactly 1 time 456381 (17.49%) aligned >1 times 95.66% overall alignment rate Time searching: 01:02:55 Overall time: 01:02:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 198857 / 18903297 = 0.0105 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:41:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:41:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:41:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:41:46: 1000000 INFO @ Tue, 02 Aug 2022 16:41:55: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:42:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:42:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:42:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:42:04: 3000000 INFO @ Tue, 02 Aug 2022 16:42:14: 4000000 INFO @ Tue, 02 Aug 2022 16:42:16: 1000000 INFO @ Tue, 02 Aug 2022 16:42:24: 5000000 INFO @ Tue, 02 Aug 2022 16:42:27: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:42:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:42:34: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:42:34: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:42:35: 6000000 INFO @ Tue, 02 Aug 2022 16:42:38: 3000000 INFO @ Tue, 02 Aug 2022 16:42:45: 7000000 INFO @ Tue, 02 Aug 2022 16:42:45: 1000000 INFO @ Tue, 02 Aug 2022 16:42:49: 4000000 INFO @ Tue, 02 Aug 2022 16:42:56: 2000000 INFO @ Tue, 02 Aug 2022 16:42:57: 8000000 INFO @ Tue, 02 Aug 2022 16:43:00: 5000000 INFO @ Tue, 02 Aug 2022 16:43:07: 3000000 INFO @ Tue, 02 Aug 2022 16:43:09: 9000000 INFO @ Tue, 02 Aug 2022 16:43:10: 6000000 INFO @ Tue, 02 Aug 2022 16:43:18: 4000000 INFO @ Tue, 02 Aug 2022 16:43:21: 7000000 INFO @ Tue, 02 Aug 2022 16:43:21: 10000000 INFO @ Tue, 02 Aug 2022 16:43:29: 5000000 INFO @ Tue, 02 Aug 2022 16:43:32: 8000000 INFO @ Tue, 02 Aug 2022 16:43:33: 11000000 INFO @ Tue, 02 Aug 2022 16:43:39: 6000000 INFO @ Tue, 02 Aug 2022 16:43:42: 9000000 INFO @ Tue, 02 Aug 2022 16:43:43: 12000000 INFO @ Tue, 02 Aug 2022 16:43:49: 7000000 INFO @ Tue, 02 Aug 2022 16:43:53: 10000000 INFO @ Tue, 02 Aug 2022 16:43:54: 13000000 INFO @ Tue, 02 Aug 2022 16:43:58: 8000000 INFO @ Tue, 02 Aug 2022 16:44:03: 11000000 INFO @ Tue, 02 Aug 2022 16:44:04: 14000000 INFO @ Tue, 02 Aug 2022 16:44:07: 9000000 INFO @ Tue, 02 Aug 2022 16:44:14: 15000000 INFO @ Tue, 02 Aug 2022 16:44:14: 12000000 INFO @ Tue, 02 Aug 2022 16:44:17: 10000000 INFO @ Tue, 02 Aug 2022 16:44:24: 16000000 INFO @ Tue, 02 Aug 2022 16:44:24: 13000000 INFO @ Tue, 02 Aug 2022 16:44:26: 11000000 INFO @ Tue, 02 Aug 2022 16:44:34: 17000000 INFO @ Tue, 02 Aug 2022 16:44:35: 14000000 INFO @ Tue, 02 Aug 2022 16:44:37: 12000000 INFO @ Tue, 02 Aug 2022 16:44:45: 18000000 INFO @ Tue, 02 Aug 2022 16:44:45: 15000000 INFO @ Tue, 02 Aug 2022 16:44:46: 13000000 INFO @ Tue, 02 Aug 2022 16:44:55: 19000000 INFO @ Tue, 02 Aug 2022 16:44:55: 14000000 INFO @ Tue, 02 Aug 2022 16:44:56: 16000000 INFO @ Tue, 02 Aug 2022 16:45:05: 15000000 INFO @ Tue, 02 Aug 2022 16:45:05: 20000000 INFO @ Tue, 02 Aug 2022 16:45:06: 17000000 INFO @ Tue, 02 Aug 2022 16:45:15: 18000000 INFO @ Tue, 02 Aug 2022 16:45:15: 16000000 INFO @ Tue, 02 Aug 2022 16:45:16: 21000000 INFO @ Tue, 02 Aug 2022 16:45:24: 19000000 INFO @ Tue, 02 Aug 2022 16:45:24: 17000000 INFO @ Tue, 02 Aug 2022 16:45:26: 22000000 INFO @ Tue, 02 Aug 2022 16:45:33: 18000000 INFO @ Tue, 02 Aug 2022 16:45:33: 20000000 INFO @ Tue, 02 Aug 2022 16:45:36: 23000000 INFO @ Tue, 02 Aug 2022 16:45:41: 19000000 INFO @ Tue, 02 Aug 2022 16:45:43: 21000000 INFO @ Tue, 02 Aug 2022 16:45:46: 24000000 INFO @ Tue, 02 Aug 2022 16:45:50: 20000000 INFO @ Tue, 02 Aug 2022 16:45:53: 22000000 INFO @ Tue, 02 Aug 2022 16:45:56: 25000000 INFO @ Tue, 02 Aug 2022 16:45:59: 21000000 INFO @ Tue, 02 Aug 2022 16:46:02: 23000000 INFO @ Tue, 02 Aug 2022 16:46:06: 26000000 INFO @ Tue, 02 Aug 2022 16:46:07: 22000000 INFO @ Tue, 02 Aug 2022 16:46:13: 24000000 INFO @ Tue, 02 Aug 2022 16:46:15: 27000000 INFO @ Tue, 02 Aug 2022 16:46:16: 23000000 INFO @ Tue, 02 Aug 2022 16:46:23: 25000000 INFO @ Tue, 02 Aug 2022 16:46:24: 28000000 INFO @ Tue, 02 Aug 2022 16:46:26: 24000000 INFO @ Tue, 02 Aug 2022 16:46:33: 26000000 INFO @ Tue, 02 Aug 2022 16:46:34: 29000000 INFO @ Tue, 02 Aug 2022 16:46:35: 25000000 INFO @ Tue, 02 Aug 2022 16:46:43: 27000000 INFO @ Tue, 02 Aug 2022 16:46:44: 30000000 INFO @ Tue, 02 Aug 2022 16:46:44: 26000000 INFO @ Tue, 02 Aug 2022 16:46:53: 31000000 INFO @ Tue, 02 Aug 2022 16:46:54: 27000000 INFO @ Tue, 02 Aug 2022 16:46:54: 28000000 INFO @ Tue, 02 Aug 2022 16:47:03: 32000000 INFO @ Tue, 02 Aug 2022 16:47:03: 28000000 INFO @ Tue, 02 Aug 2022 16:47:04: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:47:13: 29000000 INFO @ Tue, 02 Aug 2022 16:47:14: 33000000 INFO @ Tue, 02 Aug 2022 16:47:15: 30000000 INFO @ Tue, 02 Aug 2022 16:47:23: 30000000 INFO @ Tue, 02 Aug 2022 16:47:23: 34000000 INFO @ Tue, 02 Aug 2022 16:47:25: 31000000 INFO @ Tue, 02 Aug 2022 16:47:32: 31000000 INFO @ Tue, 02 Aug 2022 16:47:33: 35000000 INFO @ Tue, 02 Aug 2022 16:47:35: 32000000 INFO @ Tue, 02 Aug 2022 16:47:42: 32000000 INFO @ Tue, 02 Aug 2022 16:47:42: 36000000 INFO @ Tue, 02 Aug 2022 16:47:47: 33000000 INFO @ Tue, 02 Aug 2022 16:47:52: 37000000 INFO @ Tue, 02 Aug 2022 16:47:53: 33000000 INFO @ Tue, 02 Aug 2022 16:47:58: 34000000 INFO @ Tue, 02 Aug 2022 16:48:01: 38000000 INFO @ Tue, 02 Aug 2022 16:48:03: 34000000 INFO @ Tue, 02 Aug 2022 16:48:04: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:48:04: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:48:04: #1 total tags in treatment: 18530260 INFO @ Tue, 02 Aug 2022 16:48:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:48:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:48:05: #1 tags after filtering in treatment: 17674771 INFO @ Tue, 02 Aug 2022 16:48:05: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 16:48:05: #1 finished! INFO @ Tue, 02 Aug 2022 16:48:05: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:48:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:48:06: #2 number of paired peaks: 22 WARNING @ Tue, 02 Aug 2022 16:48:06: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:48:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:48:08: 35000000 INFO @ Tue, 02 Aug 2022 16:48:12: 35000000 INFO @ Tue, 02 Aug 2022 16:48:18: 36000000 INFO @ Tue, 02 Aug 2022 16:48:22: 36000000 INFO @ Tue, 02 Aug 2022 16:48:29: 37000000 INFO @ Tue, 02 Aug 2022 16:48:31: 37000000 INFO @ Tue, 02 Aug 2022 16:48:39: 38000000 INFO @ Tue, 02 Aug 2022 16:48:40: 38000000 INFO @ Tue, 02 Aug 2022 16:48:43: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:48:43: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:48:43: #1 total tags in treatment: 18530260 INFO @ Tue, 02 Aug 2022 16:48:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:48:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:48:43: #1 tags after filtering in treatment: 17674771 INFO @ Tue, 02 Aug 2022 16:48:43: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 16:48:43: #1 finished! INFO @ Tue, 02 Aug 2022 16:48:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:48:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:48:44: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:48:44: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:48:44: #1 total tags in treatment: 18530260 INFO @ Tue, 02 Aug 2022 16:48:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:48:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:48:44: #1 tags after filtering in treatment: 17674771 INFO @ Tue, 02 Aug 2022 16:48:44: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 16:48:44: #1 finished! INFO @ Tue, 02 Aug 2022 16:48:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:48:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:48:45: #2 number of paired peaks: 22 WARNING @ Tue, 02 Aug 2022 16:48:45: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:48:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:48:46: #2 number of paired peaks: 22 WARNING @ Tue, 02 Aug 2022 16:48:46: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:48:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9518320/SRX9518320.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。