Job ID = 14172317 SRX = SRX9281767 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 2634082 spots for SRR12813277/SRR12813277.sra Written 2634082 spots for SRR12813277/SRR12813277.sra Read 2587658 spots for SRR12813278/SRR12813278.sra Written 2587658 spots for SRR12813278/SRR12813278.sra Read 2694573 spots for SRR12813279/SRR12813279.sra Written 2694573 spots for SRR12813279/SRR12813279.sra Read 2639996 spots for SRR12813280/SRR12813280.sra Written 2639996 spots for SRR12813280/SRR12813280.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172781 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 10556309 reads; of these: 10556309 (100.00%) were paired; of these: 10240965 (97.01%) aligned concordantly 0 times 229741 (2.18%) aligned concordantly exactly 1 time 85603 (0.81%) aligned concordantly >1 times ---- 10240965 pairs aligned concordantly 0 times; of these: 740 (0.01%) aligned discordantly 1 time ---- 10240225 pairs aligned 0 times concordantly or discordantly; of these: 20480450 mates make up the pairs; of these: 20312059 (99.18%) aligned 0 times 44944 (0.22%) aligned exactly 1 time 123447 (0.60%) aligned >1 times 3.79% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7113 / 315617 = 0.0225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:10:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:10:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:10:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:10:14: #1 tag size is determined as 39 bps INFO @ Sat, 11 Dec 2021 15:10:14: #1 tag size = 39 INFO @ Sat, 11 Dec 2021 15:10:14: #1 total tags in treatment: 308243 INFO @ Sat, 11 Dec 2021 15:10:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:10:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:10:14: #1 tags after filtering in treatment: 304492 INFO @ Sat, 11 Dec 2021 15:10:14: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 11 Dec 2021 15:10:14: #1 finished! INFO @ Sat, 11 Dec 2021 15:10:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:10:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:10:14: #2 number of paired peaks: 859 WARNING @ Sat, 11 Dec 2021 15:10:14: Fewer paired peaks (859) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 859 pairs to build model! INFO @ Sat, 11 Dec 2021 15:10:14: start model_add_line... INFO @ Sat, 11 Dec 2021 15:10:14: start X-correlation... INFO @ Sat, 11 Dec 2021 15:10:14: end of X-cor INFO @ Sat, 11 Dec 2021 15:10:14: #2 finished! INFO @ Sat, 11 Dec 2021 15:10:14: #2 predicted fragment length is 100 bps INFO @ Sat, 11 Dec 2021 15:10:14: #2 alternative fragment length(s) may be 100 bps INFO @ Sat, 11 Dec 2021 15:10:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.05_model.r INFO @ Sat, 11 Dec 2021 15:10:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:10:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:10:15: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:10:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:10:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:10:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.05_summits.bed INFO @ Sat, 11 Dec 2021 15:10:15: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (80 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:10:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:10:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:10:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:10:44: #1 tag size is determined as 39 bps INFO @ Sat, 11 Dec 2021 15:10:44: #1 tag size = 39 INFO @ Sat, 11 Dec 2021 15:10:44: #1 total tags in treatment: 308243 INFO @ Sat, 11 Dec 2021 15:10:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:10:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:10:44: #1 tags after filtering in treatment: 304492 INFO @ Sat, 11 Dec 2021 15:10:44: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 11 Dec 2021 15:10:44: #1 finished! INFO @ Sat, 11 Dec 2021 15:10:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:10:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:10:44: #2 number of paired peaks: 859 WARNING @ Sat, 11 Dec 2021 15:10:44: Fewer paired peaks (859) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 859 pairs to build model! INFO @ Sat, 11 Dec 2021 15:10:44: start model_add_line... INFO @ Sat, 11 Dec 2021 15:10:44: start X-correlation... INFO @ Sat, 11 Dec 2021 15:10:44: end of X-cor INFO @ Sat, 11 Dec 2021 15:10:44: #2 finished! INFO @ Sat, 11 Dec 2021 15:10:44: #2 predicted fragment length is 100 bps INFO @ Sat, 11 Dec 2021 15:10:44: #2 alternative fragment length(s) may be 100 bps INFO @ Sat, 11 Dec 2021 15:10:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.10_model.r INFO @ Sat, 11 Dec 2021 15:10:44: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:10:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:10:45: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:10:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:10:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:10:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.10_summits.bed INFO @ Sat, 11 Dec 2021 15:10:45: Done! pass1 - making usageList (4 chroms): 0 millis pass2 - checking and writing primary data (45 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:11:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:11:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:11:10: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:11:14: #1 tag size is determined as 39 bps INFO @ Sat, 11 Dec 2021 15:11:14: #1 tag size = 39 INFO @ Sat, 11 Dec 2021 15:11:14: #1 total tags in treatment: 308243 INFO @ Sat, 11 Dec 2021 15:11:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:11:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:11:14: #1 tags after filtering in treatment: 304492 INFO @ Sat, 11 Dec 2021 15:11:14: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 11 Dec 2021 15:11:14: #1 finished! INFO @ Sat, 11 Dec 2021 15:11:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:11:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:11:14: #2 number of paired peaks: 859 WARNING @ Sat, 11 Dec 2021 15:11:14: Fewer paired peaks (859) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 859 pairs to build model! INFO @ Sat, 11 Dec 2021 15:11:14: start model_add_line... INFO @ Sat, 11 Dec 2021 15:11:14: start X-correlation... INFO @ Sat, 11 Dec 2021 15:11:14: end of X-cor INFO @ Sat, 11 Dec 2021 15:11:14: #2 finished! INFO @ Sat, 11 Dec 2021 15:11:14: #2 predicted fragment length is 100 bps INFO @ Sat, 11 Dec 2021 15:11:14: #2 alternative fragment length(s) may be 100 bps INFO @ Sat, 11 Dec 2021 15:11:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.20_model.r INFO @ Sat, 11 Dec 2021 15:11:14: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:11:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:11:14: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:11:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:11:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:11:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9281767/SRX9281767.20_summits.bed INFO @ Sat, 11 Dec 2021 15:11:15: Done! pass1 - making usageList (3 chroms): 0 millis pass2 - checking and writing primary data (35 records, 4 fields): 2 millis CompletedMACS2peakCalling