Job ID = 14171067 SRX = SRX9063530 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14222680 spots for SRR12576655/SRR12576655.sra Written 14222680 spots for SRR12576655/SRR12576655.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171501 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:02 14222680 reads; of these: 14222680 (100.00%) were unpaired; of these: 880533 (6.19%) aligned 0 times 10011116 (70.39%) aligned exactly 1 time 3331031 (23.42%) aligned >1 times 93.81% overall alignment rate Time searching: 00:06:03 Overall time: 00:06:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3094404 / 13342147 = 0.2319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:28:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:28:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:28:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:28:32: 1000000 INFO @ Sat, 11 Dec 2021 09:28:38: 2000000 INFO @ Sat, 11 Dec 2021 09:28:43: 3000000 INFO @ Sat, 11 Dec 2021 09:28:49: 4000000 INFO @ Sat, 11 Dec 2021 09:28:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:28:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:28:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:28:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:29:00: 6000000 INFO @ Sat, 11 Dec 2021 09:29:03: 1000000 INFO @ Sat, 11 Dec 2021 09:29:06: 7000000 INFO @ Sat, 11 Dec 2021 09:29:09: 2000000 INFO @ Sat, 11 Dec 2021 09:29:12: 8000000 INFO @ Sat, 11 Dec 2021 09:29:15: 3000000 INFO @ Sat, 11 Dec 2021 09:29:18: 9000000 INFO @ Sat, 11 Dec 2021 09:29:21: 4000000 INFO @ Sat, 11 Dec 2021 09:29:23: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:29:25: #1 tag size is determined as 76 bps INFO @ Sat, 11 Dec 2021 09:29:25: #1 tag size = 76 INFO @ Sat, 11 Dec 2021 09:29:25: #1 total tags in treatment: 10247743 INFO @ Sat, 11 Dec 2021 09:29:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:29:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:29:25: #1 tags after filtering in treatment: 10247743 INFO @ Sat, 11 Dec 2021 09:29:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:29:25: #1 finished! INFO @ Sat, 11 Dec 2021 09:29:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:29:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:29:26: #2 number of paired peaks: 83 WARNING @ Sat, 11 Dec 2021 09:29:26: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:29:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:29:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:29:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:29:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:29:27: 5000000 INFO @ Sat, 11 Dec 2021 09:29:33: 1000000 INFO @ Sat, 11 Dec 2021 09:29:33: 6000000 INFO @ Sat, 11 Dec 2021 09:29:39: 2000000 INFO @ Sat, 11 Dec 2021 09:29:39: 7000000 INFO @ Sat, 11 Dec 2021 09:29:45: 3000000 INFO @ Sat, 11 Dec 2021 09:29:45: 8000000 INFO @ Sat, 11 Dec 2021 09:29:51: 4000000 INFO @ Sat, 11 Dec 2021 09:29:51: 9000000 INFO @ Sat, 11 Dec 2021 09:29:57: 5000000 INFO @ Sat, 11 Dec 2021 09:29:57: 10000000 INFO @ Sat, 11 Dec 2021 09:29:58: #1 tag size is determined as 76 bps INFO @ Sat, 11 Dec 2021 09:29:58: #1 tag size = 76 INFO @ Sat, 11 Dec 2021 09:29:58: #1 total tags in treatment: 10247743 INFO @ Sat, 11 Dec 2021 09:29:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:29:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:29:59: #1 tags after filtering in treatment: 10247743 INFO @ Sat, 11 Dec 2021 09:29:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:29:59: #1 finished! INFO @ Sat, 11 Dec 2021 09:29:59: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:29:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:29:59: #2 number of paired peaks: 83 WARNING @ Sat, 11 Dec 2021 09:29:59: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:29:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:30:02: 6000000 INFO @ Sat, 11 Dec 2021 09:30:08: 7000000 INFO @ Sat, 11 Dec 2021 09:30:13: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:30:19: 9000000 INFO @ Sat, 11 Dec 2021 09:30:25: 10000000 INFO @ Sat, 11 Dec 2021 09:30:26: #1 tag size is determined as 76 bps INFO @ Sat, 11 Dec 2021 09:30:26: #1 tag size = 76 INFO @ Sat, 11 Dec 2021 09:30:26: #1 total tags in treatment: 10247743 INFO @ Sat, 11 Dec 2021 09:30:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:30:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:30:26: #1 tags after filtering in treatment: 10247743 INFO @ Sat, 11 Dec 2021 09:30:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:30:26: #1 finished! INFO @ Sat, 11 Dec 2021 09:30:26: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:30:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:30:27: #2 number of paired peaks: 83 WARNING @ Sat, 11 Dec 2021 09:30:27: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:30:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063530/SRX9063530.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。