Job ID = 14171066 SRX = SRX9063529 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13164019 spots for SRR12576654/SRR12576654.sra Written 13164019 spots for SRR12576654/SRR12576654.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171502 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:12 13164019 reads; of these: 13164019 (100.00%) were unpaired; of these: 805057 (6.12%) aligned 0 times 9190869 (69.82%) aligned exactly 1 time 3168093 (24.07%) aligned >1 times 93.88% overall alignment rate Time searching: 00:06:13 Overall time: 00:06:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2791238 / 12358962 = 0.2258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:28:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:28:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:28:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:28:41: 1000000 INFO @ Sat, 11 Dec 2021 09:28:46: 2000000 INFO @ Sat, 11 Dec 2021 09:28:52: 3000000 INFO @ Sat, 11 Dec 2021 09:28:58: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:29:03: 5000000 INFO @ Sat, 11 Dec 2021 09:29:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:29:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:29:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:29:09: 6000000 INFO @ Sat, 11 Dec 2021 09:29:11: 1000000 INFO @ Sat, 11 Dec 2021 09:29:15: 7000000 INFO @ Sat, 11 Dec 2021 09:29:17: 2000000 INFO @ Sat, 11 Dec 2021 09:29:21: 8000000 INFO @ Sat, 11 Dec 2021 09:29:23: 3000000 INFO @ Sat, 11 Dec 2021 09:29:26: 9000000 INFO @ Sat, 11 Dec 2021 09:29:28: 4000000 INFO @ Sat, 11 Dec 2021 09:29:30: #1 tag size is determined as 76 bps INFO @ Sat, 11 Dec 2021 09:29:30: #1 tag size = 76 INFO @ Sat, 11 Dec 2021 09:29:30: #1 total tags in treatment: 9567724 INFO @ Sat, 11 Dec 2021 09:29:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:29:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:29:30: #1 tags after filtering in treatment: 9567724 INFO @ Sat, 11 Dec 2021 09:29:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:29:30: #1 finished! INFO @ Sat, 11 Dec 2021 09:29:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:29:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:29:31: #2 number of paired peaks: 88 WARNING @ Sat, 11 Dec 2021 09:29:31: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:29:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:29:34: 5000000 INFO @ Sat, 11 Dec 2021 09:29:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:29:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:29:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:29:39: 6000000 INFO @ Sat, 11 Dec 2021 09:29:41: 1000000 INFO @ Sat, 11 Dec 2021 09:29:45: 7000000 INFO @ Sat, 11 Dec 2021 09:29:47: 2000000 INFO @ Sat, 11 Dec 2021 09:29:51: 8000000 INFO @ Sat, 11 Dec 2021 09:29:53: 3000000 INFO @ Sat, 11 Dec 2021 09:29:57: 9000000 INFO @ Sat, 11 Dec 2021 09:29:59: 4000000 INFO @ Sat, 11 Dec 2021 09:30:00: #1 tag size is determined as 76 bps INFO @ Sat, 11 Dec 2021 09:30:00: #1 tag size = 76 INFO @ Sat, 11 Dec 2021 09:30:00: #1 total tags in treatment: 9567724 INFO @ Sat, 11 Dec 2021 09:30:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:30:00: #1 tags after filtering in treatment: 9567724 INFO @ Sat, 11 Dec 2021 09:30:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:30:00: #1 finished! INFO @ Sat, 11 Dec 2021 09:30:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:30:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:30:01: #2 number of paired peaks: 88 WARNING @ Sat, 11 Dec 2021 09:30:01: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:30:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:30:04: 5000000 INFO @ Sat, 11 Dec 2021 09:30:10: 6000000 INFO @ Sat, 11 Dec 2021 09:30:15: 7000000 INFO @ Sat, 11 Dec 2021 09:30:21: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:30:26: 9000000 INFO @ Sat, 11 Dec 2021 09:30:30: #1 tag size is determined as 76 bps INFO @ Sat, 11 Dec 2021 09:30:30: #1 tag size = 76 INFO @ Sat, 11 Dec 2021 09:30:30: #1 total tags in treatment: 9567724 INFO @ Sat, 11 Dec 2021 09:30:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:30:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:30:30: #1 tags after filtering in treatment: 9567724 INFO @ Sat, 11 Dec 2021 09:30:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:30:30: #1 finished! INFO @ Sat, 11 Dec 2021 09:30:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:30:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:30:30: #2 number of paired peaks: 88 WARNING @ Sat, 11 Dec 2021 09:30:30: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 09:30:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9063529/SRX9063529.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。