Job ID = 14171546 SRX = SRX9008801 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 30139461 spots for SRR12518273/SRR12518273.sra Written 30139461 spots for SRR12518273/SRR12518273.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172014 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:03 30139461 reads; of these: 30139461 (100.00%) were unpaired; of these: 11494938 (38.14%) aligned 0 times 14675716 (48.69%) aligned exactly 1 time 3968807 (13.17%) aligned >1 times 61.86% overall alignment rate Time searching: 00:07:03 Overall time: 00:07:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2925753 / 18644523 = 0.1569 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:10:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:10:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:10:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:11:00: 1000000 INFO @ Sat, 11 Dec 2021 12:11:05: 2000000 INFO @ Sat, 11 Dec 2021 12:11:10: 3000000 INFO @ Sat, 11 Dec 2021 12:11:16: 4000000 INFO @ Sat, 11 Dec 2021 12:11:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:11:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:11:25: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:11:25: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:11:26: 6000000 INFO @ Sat, 11 Dec 2021 12:11:30: 1000000 INFO @ Sat, 11 Dec 2021 12:11:32: 7000000 INFO @ Sat, 11 Dec 2021 12:11:35: 2000000 INFO @ Sat, 11 Dec 2021 12:11:37: 8000000 INFO @ Sat, 11 Dec 2021 12:11:41: 3000000 INFO @ Sat, 11 Dec 2021 12:11:43: 9000000 INFO @ Sat, 11 Dec 2021 12:11:46: 4000000 INFO @ Sat, 11 Dec 2021 12:11:48: 10000000 INFO @ Sat, 11 Dec 2021 12:11:52: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:11:53: 11000000 INFO @ Sat, 11 Dec 2021 12:11:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:11:55: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:11:55: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:11:57: 6000000 INFO @ Sat, 11 Dec 2021 12:11:59: 12000000 INFO @ Sat, 11 Dec 2021 12:12:00: 1000000 INFO @ Sat, 11 Dec 2021 12:12:03: 7000000 INFO @ Sat, 11 Dec 2021 12:12:05: 2000000 INFO @ Sat, 11 Dec 2021 12:12:05: 13000000 INFO @ Sat, 11 Dec 2021 12:12:08: 8000000 INFO @ Sat, 11 Dec 2021 12:12:10: 3000000 INFO @ Sat, 11 Dec 2021 12:12:10: 14000000 INFO @ Sat, 11 Dec 2021 12:12:14: 9000000 INFO @ Sat, 11 Dec 2021 12:12:14: 4000000 INFO @ Sat, 11 Dec 2021 12:12:16: 15000000 INFO @ Sat, 11 Dec 2021 12:12:19: 5000000 INFO @ Sat, 11 Dec 2021 12:12:20: 10000000 INFO @ Sat, 11 Dec 2021 12:12:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:12:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:12:20: #1 total tags in treatment: 15718770 INFO @ Sat, 11 Dec 2021 12:12:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:12:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:12:21: #1 tags after filtering in treatment: 15718770 INFO @ Sat, 11 Dec 2021 12:12:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:12:21: #1 finished! INFO @ Sat, 11 Dec 2021 12:12:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:12:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:12:22: #2 number of paired peaks: 33 WARNING @ Sat, 11 Dec 2021 12:12:22: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:12:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:12:24: 6000000 INFO @ Sat, 11 Dec 2021 12:12:25: 11000000 INFO @ Sat, 11 Dec 2021 12:12:29: 7000000 INFO @ Sat, 11 Dec 2021 12:12:31: 12000000 INFO @ Sat, 11 Dec 2021 12:12:33: 8000000 INFO @ Sat, 11 Dec 2021 12:12:36: 13000000 INFO @ Sat, 11 Dec 2021 12:12:38: 9000000 INFO @ Sat, 11 Dec 2021 12:12:42: 14000000 INFO @ Sat, 11 Dec 2021 12:12:43: 10000000 INFO @ Sat, 11 Dec 2021 12:12:48: 15000000 INFO @ Sat, 11 Dec 2021 12:12:48: 11000000 INFO @ Sat, 11 Dec 2021 12:12:52: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:12:52: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:12:52: #1 total tags in treatment: 15718770 INFO @ Sat, 11 Dec 2021 12:12:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:12:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:12:52: #1 tags after filtering in treatment: 15718770 INFO @ Sat, 11 Dec 2021 12:12:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:12:52: #1 finished! INFO @ Sat, 11 Dec 2021 12:12:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:12:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:12:52: 12000000 INFO @ Sat, 11 Dec 2021 12:12:53: #2 number of paired peaks: 33 WARNING @ Sat, 11 Dec 2021 12:12:53: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:12:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 11 Dec 2021 12:12:57: 13000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:13:02: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:13:07: 15000000 INFO @ Sat, 11 Dec 2021 12:13:10: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 12:13:10: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 12:13:10: #1 total tags in treatment: 15718770 INFO @ Sat, 11 Dec 2021 12:13:10: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:13:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:13:11: #1 tags after filtering in treatment: 15718770 INFO @ Sat, 11 Dec 2021 12:13:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 12:13:11: #1 finished! INFO @ Sat, 11 Dec 2021 12:13:11: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:13:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:13:12: #2 number of paired peaks: 33 WARNING @ Sat, 11 Dec 2021 12:13:12: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 12:13:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008801/SRX9008801.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。