Job ID = 10166259 SRX = SRX9008800 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21451417 spots for SRR12518272/SRR12518272.sra Written 21451417 spots for SRR12518272/SRR12518272.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166617 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:47 21451417 reads; of these: 21451417 (100.00%) were unpaired; of these: 4724631 (22.02%) aligned 0 times 14164712 (66.03%) aligned exactly 1 time 2562074 (11.94%) aligned >1 times 77.98% overall alignment rate Time searching: 00:05:47 Overall time: 00:05:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2993683 / 16726786 = 0.1790 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:55:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:55:25: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:55:25: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:55:30: 1000000 INFO @ Thu, 08 Oct 2020 20:55:35: 2000000 INFO @ Thu, 08 Oct 2020 20:55:40: 3000000 INFO @ Thu, 08 Oct 2020 20:55:45: 4000000 INFO @ Thu, 08 Oct 2020 20:55:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:55:54: 6000000 INFO @ Thu, 08 Oct 2020 20:55:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:55:55: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:55:55: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:55:59: 7000000 INFO @ Thu, 08 Oct 2020 20:56:01: 1000000 INFO @ Thu, 08 Oct 2020 20:56:04: 8000000 INFO @ Thu, 08 Oct 2020 20:56:06: 2000000 INFO @ Thu, 08 Oct 2020 20:56:09: 9000000 INFO @ Thu, 08 Oct 2020 20:56:11: 3000000 INFO @ Thu, 08 Oct 2020 20:56:14: 10000000 INFO @ Thu, 08 Oct 2020 20:56:16: 4000000 INFO @ Thu, 08 Oct 2020 20:56:19: 11000000 INFO @ Thu, 08 Oct 2020 20:56:20: 5000000 INFO @ Thu, 08 Oct 2020 20:56:23: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:56:25: 6000000 INFO @ Thu, 08 Oct 2020 20:56:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:56:25: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:56:25: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:56:28: 13000000 INFO @ Thu, 08 Oct 2020 20:56:30: 7000000 INFO @ Thu, 08 Oct 2020 20:56:31: 1000000 INFO @ Thu, 08 Oct 2020 20:56:32: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:56:32: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:56:32: #1 total tags in treatment: 13733103 INFO @ Thu, 08 Oct 2020 20:56:32: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:56:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:56:32: #1 tags after filtering in treatment: 13733103 INFO @ Thu, 08 Oct 2020 20:56:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:56:32: #1 finished! INFO @ Thu, 08 Oct 2020 20:56:32: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:56:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:56:33: #2 number of paired peaks: 50 WARNING @ Thu, 08 Oct 2020 20:56:33: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:56:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:56:35: 8000000 INFO @ Thu, 08 Oct 2020 20:56:36: 2000000 INFO @ Thu, 08 Oct 2020 20:56:39: 9000000 INFO @ Thu, 08 Oct 2020 20:56:42: 3000000 INFO @ Thu, 08 Oct 2020 20:56:44: 10000000 INFO @ Thu, 08 Oct 2020 20:56:47: 4000000 INFO @ Thu, 08 Oct 2020 20:56:49: 11000000 INFO @ Thu, 08 Oct 2020 20:56:53: 5000000 INFO @ Thu, 08 Oct 2020 20:56:54: 12000000 INFO @ Thu, 08 Oct 2020 20:56:58: 6000000 INFO @ Thu, 08 Oct 2020 20:56:58: 13000000 INFO @ Thu, 08 Oct 2020 20:57:02: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:57:02: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:57:02: #1 total tags in treatment: 13733103 INFO @ Thu, 08 Oct 2020 20:57:02: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:57:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:57:02: #1 tags after filtering in treatment: 13733103 INFO @ Thu, 08 Oct 2020 20:57:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:57:02: #1 finished! INFO @ Thu, 08 Oct 2020 20:57:02: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:57:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:57:03: #2 number of paired peaks: 50 WARNING @ Thu, 08 Oct 2020 20:57:03: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:57:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:57:03: 7000000 INFO @ Thu, 08 Oct 2020 20:57:08: 8000000 INFO @ Thu, 08 Oct 2020 20:57:14: 9000000 INFO @ Thu, 08 Oct 2020 20:57:19: 10000000 INFO @ Thu, 08 Oct 2020 20:57:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:57:29: 12000000 INFO @ Thu, 08 Oct 2020 20:57:34: 13000000 INFO @ Thu, 08 Oct 2020 20:57:38: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:57:38: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:57:38: #1 total tags in treatment: 13733103 INFO @ Thu, 08 Oct 2020 20:57:38: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:57:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:57:38: #1 tags after filtering in treatment: 13733103 INFO @ Thu, 08 Oct 2020 20:57:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:57:38: #1 finished! INFO @ Thu, 08 Oct 2020 20:57:38: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:57:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:57:39: #2 number of paired peaks: 50 WARNING @ Thu, 08 Oct 2020 20:57:39: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:57:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX9008800/SRX9008800.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。