Job ID = 14171380 SRX = SRX9005691 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 472826 READS because READLEN < 1 Read 2234438 spots for SRR12515092/SRR12515092.sra Written 2234438 spots for SRR12515092/SRR12515092.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171837 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6321 / 48921 = 0.1292 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:31:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:31:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:31:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:31:22: #1 tag size is determined as 97 bps INFO @ Sat, 11 Dec 2021 11:31:22: #1 tag size = 97 INFO @ Sat, 11 Dec 2021 11:31:22: #1 total tags in treatment: 41081 INFO @ Sat, 11 Dec 2021 11:31:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:31:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:31:22: #1 tags after filtering in treatment: 40229 INFO @ Sat, 11 Dec 2021 11:31:22: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 11 Dec 2021 11:31:22: #1 finished! INFO @ Sat, 11 Dec 2021 11:31:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:31:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:31:22: #2 number of paired peaks: 1491 INFO @ Sat, 11 Dec 2021 11:31:22: start model_add_line... INFO @ Sat, 11 Dec 2021 11:31:22: start X-correlation... INFO @ Sat, 11 Dec 2021 11:31:22: end of X-cor INFO @ Sat, 11 Dec 2021 11:31:22: #2 finished! INFO @ Sat, 11 Dec 2021 11:31:22: #2 predicted fragment length is 296 bps INFO @ Sat, 11 Dec 2021 11:31:22: #2 alternative fragment length(s) may be 220,275,296 bps INFO @ Sat, 11 Dec 2021 11:31:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.05_model.r INFO @ Sat, 11 Dec 2021 11:31:22: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:31:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:31:22: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:31:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.05_peaks.xls INFO @ Sat, 11 Dec 2021 11:31:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:31:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.05_summits.bed INFO @ Sat, 11 Dec 2021 11:31:22: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:31:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:31:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:31:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 11:31:52: #1 tag size is determined as 97 bps INFO @ Sat, 11 Dec 2021 11:31:52: #1 tag size = 97 INFO @ Sat, 11 Dec 2021 11:31:52: #1 total tags in treatment: 41081 INFO @ Sat, 11 Dec 2021 11:31:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:31:52: #1 tags after filtering in treatment: 40229 INFO @ Sat, 11 Dec 2021 11:31:52: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 11 Dec 2021 11:31:52: #1 finished! INFO @ Sat, 11 Dec 2021 11:31:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:31:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:31:52: #2 number of paired peaks: 1491 INFO @ Sat, 11 Dec 2021 11:31:52: start model_add_line... INFO @ Sat, 11 Dec 2021 11:31:52: start X-correlation... INFO @ Sat, 11 Dec 2021 11:31:52: end of X-cor INFO @ Sat, 11 Dec 2021 11:31:52: #2 finished! INFO @ Sat, 11 Dec 2021 11:31:52: #2 predicted fragment length is 296 bps INFO @ Sat, 11 Dec 2021 11:31:52: #2 alternative fragment length(s) may be 220,275,296 bps INFO @ Sat, 11 Dec 2021 11:31:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.10_model.r INFO @ Sat, 11 Dec 2021 11:31:52: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:31:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 11:31:52: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:31:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.10_peaks.xls INFO @ Sat, 11 Dec 2021 11:31:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:31:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.10_summits.bed INFO @ Sat, 11 Dec 2021 11:31:52: Done! pass1 - making usageList (1 chroms): 0 millis pass2 - checking and writing primary data (1 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 11:32:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 11:32:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 11:32:19: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 11:32:22: #1 tag size is determined as 97 bps INFO @ Sat, 11 Dec 2021 11:32:22: #1 tag size = 97 INFO @ Sat, 11 Dec 2021 11:32:22: #1 total tags in treatment: 41081 INFO @ Sat, 11 Dec 2021 11:32:22: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 11:32:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 11:32:22: #1 tags after filtering in treatment: 40229 INFO @ Sat, 11 Dec 2021 11:32:22: #1 Redundant rate of treatment: 0.02 INFO @ Sat, 11 Dec 2021 11:32:22: #1 finished! INFO @ Sat, 11 Dec 2021 11:32:22: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 11:32:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 11:32:22: #2 number of paired peaks: 1491 INFO @ Sat, 11 Dec 2021 11:32:22: start model_add_line... INFO @ Sat, 11 Dec 2021 11:32:22: start X-correlation... INFO @ Sat, 11 Dec 2021 11:32:22: end of X-cor INFO @ Sat, 11 Dec 2021 11:32:22: #2 finished! INFO @ Sat, 11 Dec 2021 11:32:22: #2 predicted fragment length is 296 bps INFO @ Sat, 11 Dec 2021 11:32:22: #2 alternative fragment length(s) may be 220,275,296 bps INFO @ Sat, 11 Dec 2021 11:32:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.20_model.r INFO @ Sat, 11 Dec 2021 11:32:22: #3 Call peaks... INFO @ Sat, 11 Dec 2021 11:32:22: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 11:32:22: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 11:32:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.20_peaks.xls INFO @ Sat, 11 Dec 2021 11:32:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 11:32:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX9005691/SRX9005691.20_summits.bed INFO @ Sat, 11 Dec 2021 11:32:22: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling