Job ID = 14166943 SRX = SRX8755942 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28278679 spots for SRR12248079/SRR12248079.sra Written 28278679 spots for SRR12248079/SRR12248079.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167320 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:06 28278679 reads; of these: 28278679 (100.00%) were paired; of these: 7989391 (28.25%) aligned concordantly 0 times 18112485 (64.05%) aligned concordantly exactly 1 time 2176803 (7.70%) aligned concordantly >1 times ---- 7989391 pairs aligned concordantly 0 times; of these: 3151901 (39.45%) aligned discordantly 1 time ---- 4837490 pairs aligned 0 times concordantly or discordantly; of these: 9674980 mates make up the pairs; of these: 7817131 (80.80%) aligned 0 times 1192471 (12.33%) aligned exactly 1 time 665378 (6.88%) aligned >1 times 86.18% overall alignment rate Time searching: 00:26:06 Overall time: 00:26:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3385055 / 23069660 = 0.1467 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:44:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:44:41: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:44:41: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:44:48: 1000000 INFO @ Fri, 10 Dec 2021 08:44:55: 2000000 INFO @ Fri, 10 Dec 2021 08:45:02: 3000000 INFO @ Fri, 10 Dec 2021 08:45:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:45:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:45:11: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:45:11: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:45:16: 5000000 INFO @ Fri, 10 Dec 2021 08:45:18: 1000000 INFO @ Fri, 10 Dec 2021 08:45:23: 6000000 INFO @ Fri, 10 Dec 2021 08:45:26: 2000000 INFO @ Fri, 10 Dec 2021 08:45:31: 7000000 INFO @ Fri, 10 Dec 2021 08:45:33: 3000000 INFO @ Fri, 10 Dec 2021 08:45:39: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:45:40: 4000000 INFO @ Fri, 10 Dec 2021 08:45:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:45:41: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:45:41: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:45:46: 9000000 INFO @ Fri, 10 Dec 2021 08:45:47: 5000000 INFO @ Fri, 10 Dec 2021 08:45:49: 1000000 INFO @ Fri, 10 Dec 2021 08:45:54: 10000000 INFO @ Fri, 10 Dec 2021 08:45:55: 6000000 INFO @ Fri, 10 Dec 2021 08:45:56: 2000000 INFO @ Fri, 10 Dec 2021 08:46:02: 11000000 INFO @ Fri, 10 Dec 2021 08:46:02: 7000000 INFO @ Fri, 10 Dec 2021 08:46:04: 3000000 INFO @ Fri, 10 Dec 2021 08:46:09: 12000000 INFO @ Fri, 10 Dec 2021 08:46:10: 8000000 INFO @ Fri, 10 Dec 2021 08:46:11: 4000000 INFO @ Fri, 10 Dec 2021 08:46:17: 13000000 INFO @ Fri, 10 Dec 2021 08:46:17: 9000000 INFO @ Fri, 10 Dec 2021 08:46:19: 5000000 INFO @ Fri, 10 Dec 2021 08:46:24: 10000000 INFO @ Fri, 10 Dec 2021 08:46:25: 14000000 INFO @ Fri, 10 Dec 2021 08:46:26: 6000000 INFO @ Fri, 10 Dec 2021 08:46:32: 11000000 INFO @ Fri, 10 Dec 2021 08:46:33: 15000000 INFO @ Fri, 10 Dec 2021 08:46:34: 7000000 INFO @ Fri, 10 Dec 2021 08:46:39: 12000000 INFO @ Fri, 10 Dec 2021 08:46:40: 16000000 INFO @ Fri, 10 Dec 2021 08:46:42: 8000000 INFO @ Fri, 10 Dec 2021 08:46:47: 13000000 INFO @ Fri, 10 Dec 2021 08:46:48: 17000000 INFO @ Fri, 10 Dec 2021 08:46:49: 9000000 INFO @ Fri, 10 Dec 2021 08:46:54: 14000000 INFO @ Fri, 10 Dec 2021 08:46:56: 18000000 INFO @ Fri, 10 Dec 2021 08:46:57: 10000000 INFO @ Fri, 10 Dec 2021 08:47:02: 15000000 INFO @ Fri, 10 Dec 2021 08:47:04: 19000000 INFO @ Fri, 10 Dec 2021 08:47:04: 11000000 INFO @ Fri, 10 Dec 2021 08:47:09: 16000000 INFO @ Fri, 10 Dec 2021 08:47:11: 20000000 INFO @ Fri, 10 Dec 2021 08:47:12: 12000000 INFO @ Fri, 10 Dec 2021 08:47:17: 17000000 INFO @ Fri, 10 Dec 2021 08:47:19: 21000000 INFO @ Fri, 10 Dec 2021 08:47:19: 13000000 INFO @ Fri, 10 Dec 2021 08:47:24: 18000000 INFO @ Fri, 10 Dec 2021 08:47:27: 22000000 INFO @ Fri, 10 Dec 2021 08:47:27: 14000000 INFO @ Fri, 10 Dec 2021 08:47:31: 19000000 INFO @ Fri, 10 Dec 2021 08:47:34: 23000000 INFO @ Fri, 10 Dec 2021 08:47:35: 15000000 INFO @ Fri, 10 Dec 2021 08:47:39: 20000000 INFO @ Fri, 10 Dec 2021 08:47:42: 24000000 INFO @ Fri, 10 Dec 2021 08:47:42: 16000000 INFO @ Fri, 10 Dec 2021 08:47:46: 21000000 INFO @ Fri, 10 Dec 2021 08:47:50: 25000000 INFO @ Fri, 10 Dec 2021 08:47:50: 17000000 INFO @ Fri, 10 Dec 2021 08:47:54: 22000000 INFO @ Fri, 10 Dec 2021 08:47:58: 26000000 INFO @ Fri, 10 Dec 2021 08:47:58: 18000000 INFO @ Fri, 10 Dec 2021 08:48:02: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:48:05: 27000000 INFO @ Fri, 10 Dec 2021 08:48:05: 19000000 INFO @ Fri, 10 Dec 2021 08:48:09: 24000000 INFO @ Fri, 10 Dec 2021 08:48:13: 28000000 INFO @ Fri, 10 Dec 2021 08:48:13: 20000000 INFO @ Fri, 10 Dec 2021 08:48:17: 25000000 INFO @ Fri, 10 Dec 2021 08:48:21: 29000000 INFO @ Fri, 10 Dec 2021 08:48:21: 21000000 INFO @ Fri, 10 Dec 2021 08:48:24: 26000000 INFO @ Fri, 10 Dec 2021 08:48:29: 30000000 INFO @ Fri, 10 Dec 2021 08:48:29: 22000000 INFO @ Fri, 10 Dec 2021 08:48:32: 27000000 INFO @ Fri, 10 Dec 2021 08:48:36: 23000000 INFO @ Fri, 10 Dec 2021 08:48:36: 31000000 INFO @ Fri, 10 Dec 2021 08:48:39: 28000000 INFO @ Fri, 10 Dec 2021 08:48:44: 24000000 INFO @ Fri, 10 Dec 2021 08:48:44: 32000000 INFO @ Fri, 10 Dec 2021 08:48:47: 29000000 INFO @ Fri, 10 Dec 2021 08:48:52: 25000000 INFO @ Fri, 10 Dec 2021 08:48:52: 33000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:48:55: 30000000 INFO @ Fri, 10 Dec 2021 08:48:59: 34000000 INFO @ Fri, 10 Dec 2021 08:49:00: 26000000 INFO @ Fri, 10 Dec 2021 08:49:02: 31000000 INFO @ Fri, 10 Dec 2021 08:49:07: 35000000 INFO @ Fri, 10 Dec 2021 08:49:07: 27000000 INFO @ Fri, 10 Dec 2021 08:49:10: 32000000 INFO @ Fri, 10 Dec 2021 08:49:14: 36000000 INFO @ Fri, 10 Dec 2021 08:49:15: 28000000 INFO @ Fri, 10 Dec 2021 08:49:18: 33000000 INFO @ Fri, 10 Dec 2021 08:49:22: 37000000 INFO @ Fri, 10 Dec 2021 08:49:23: 29000000 INFO @ Fri, 10 Dec 2021 08:49:25: 34000000 INFO @ Fri, 10 Dec 2021 08:49:30: 38000000 INFO @ Fri, 10 Dec 2021 08:49:31: 30000000 INFO @ Fri, 10 Dec 2021 08:49:33: 35000000 INFO @ Fri, 10 Dec 2021 08:49:37: 39000000 INFO @ Fri, 10 Dec 2021 08:49:39: 31000000 INFO @ Fri, 10 Dec 2021 08:49:40: 36000000 INFO @ Fri, 10 Dec 2021 08:49:45: 40000000 INFO @ Fri, 10 Dec 2021 08:49:46: 32000000 INFO @ Fri, 10 Dec 2021 08:49:48: 37000000 INFO @ Fri, 10 Dec 2021 08:49:52: 41000000 INFO @ Fri, 10 Dec 2021 08:49:54: 33000000 INFO @ Fri, 10 Dec 2021 08:49:55: 38000000 INFO @ Fri, 10 Dec 2021 08:49:59: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 08:49:59: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 08:49:59: #1 total tags in treatment: 17542911 INFO @ Fri, 10 Dec 2021 08:49:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:49:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:50:00: #1 tags after filtering in treatment: 11014906 INFO @ Fri, 10 Dec 2021 08:50:00: #1 Redundant rate of treatment: 0.37 INFO @ Fri, 10 Dec 2021 08:50:00: #1 finished! INFO @ Fri, 10 Dec 2021 08:50:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:50:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:50:01: #2 number of paired peaks: 3680 INFO @ Fri, 10 Dec 2021 08:50:01: start model_add_line... INFO @ Fri, 10 Dec 2021 08:50:01: start X-correlation... INFO @ Fri, 10 Dec 2021 08:50:01: end of X-cor INFO @ Fri, 10 Dec 2021 08:50:01: #2 finished! INFO @ Fri, 10 Dec 2021 08:50:01: #2 predicted fragment length is 66 bps INFO @ Fri, 10 Dec 2021 08:50:01: #2 alternative fragment length(s) may be 66 bps INFO @ Fri, 10 Dec 2021 08:50:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.05_model.r WARNING @ Fri, 10 Dec 2021 08:50:01: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 08:50:01: #2 You may need to consider one of the other alternative d(s): 66 WARNING @ Fri, 10 Dec 2021 08:50:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 08:50:01: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:50:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:50:02: 34000000 INFO @ Fri, 10 Dec 2021 08:50:03: 39000000 INFO @ Fri, 10 Dec 2021 08:50:09: 35000000 INFO @ Fri, 10 Dec 2021 08:50:10: 40000000 INFO @ Fri, 10 Dec 2021 08:50:17: 36000000 INFO @ Fri, 10 Dec 2021 08:50:17: 41000000 INFO @ Fri, 10 Dec 2021 08:50:23: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:50:24: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 08:50:24: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 08:50:24: #1 total tags in treatment: 17542911 INFO @ Fri, 10 Dec 2021 08:50:24: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:50:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:50:24: 37000000 INFO @ Fri, 10 Dec 2021 08:50:25: #1 tags after filtering in treatment: 11014906 INFO @ Fri, 10 Dec 2021 08:50:25: #1 Redundant rate of treatment: 0.37 INFO @ Fri, 10 Dec 2021 08:50:25: #1 finished! INFO @ Fri, 10 Dec 2021 08:50:25: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:50:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:50:26: #2 number of paired peaks: 3680 INFO @ Fri, 10 Dec 2021 08:50:26: start model_add_line... INFO @ Fri, 10 Dec 2021 08:50:26: start X-correlation... INFO @ Fri, 10 Dec 2021 08:50:26: end of X-cor INFO @ Fri, 10 Dec 2021 08:50:26: #2 finished! INFO @ Fri, 10 Dec 2021 08:50:26: #2 predicted fragment length is 66 bps INFO @ Fri, 10 Dec 2021 08:50:26: #2 alternative fragment length(s) may be 66 bps INFO @ Fri, 10 Dec 2021 08:50:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.10_model.r WARNING @ Fri, 10 Dec 2021 08:50:26: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 08:50:26: #2 You may need to consider one of the other alternative d(s): 66 WARNING @ Fri, 10 Dec 2021 08:50:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 08:50:26: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:50:26: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:50:31: 38000000 INFO @ Fri, 10 Dec 2021 08:50:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.05_peaks.xls INFO @ Fri, 10 Dec 2021 08:50:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:50:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.05_summits.bed INFO @ Fri, 10 Dec 2021 08:50:35: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (20726 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:50:38: 39000000 INFO @ Fri, 10 Dec 2021 08:50:45: 40000000 INFO @ Fri, 10 Dec 2021 08:50:48: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:50:52: 41000000 INFO @ Fri, 10 Dec 2021 08:50:59: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 08:50:59: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 08:50:59: #1 total tags in treatment: 17542911 INFO @ Fri, 10 Dec 2021 08:50:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:50:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:50:59: #1 tags after filtering in treatment: 11014906 INFO @ Fri, 10 Dec 2021 08:50:59: #1 Redundant rate of treatment: 0.37 INFO @ Fri, 10 Dec 2021 08:50:59: #1 finished! INFO @ Fri, 10 Dec 2021 08:50:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:50:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:50:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.10_peaks.xls INFO @ Fri, 10 Dec 2021 08:50:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:51:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.10_summits.bed INFO @ Fri, 10 Dec 2021 08:51:00: Done! INFO @ Fri, 10 Dec 2021 08:51:00: #2 number of paired peaks: 3680 INFO @ Fri, 10 Dec 2021 08:51:00: start model_add_line... INFO @ Fri, 10 Dec 2021 08:51:00: start X-correlation... INFO @ Fri, 10 Dec 2021 08:51:00: end of X-cor INFO @ Fri, 10 Dec 2021 08:51:00: #2 finished! INFO @ Fri, 10 Dec 2021 08:51:00: #2 predicted fragment length is 66 bps INFO @ Fri, 10 Dec 2021 08:51:00: #2 alternative fragment length(s) may be 66 bps INFO @ Fri, 10 Dec 2021 08:51:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.20_model.r WARNING @ Fri, 10 Dec 2021 08:51:00: #2 Since the d (66) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 08:51:00: #2 You may need to consider one of the other alternative d(s): 66 WARNING @ Fri, 10 Dec 2021 08:51:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 08:51:00: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:51:00: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (12728 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:51:21: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:51:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.20_peaks.xls INFO @ Fri, 10 Dec 2021 08:51:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:51:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8755942/SRX8755942.20_summits.bed INFO @ Fri, 10 Dec 2021 08:51:32: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (4547 records, 4 fields): 5 millis CompletedMACS2peakCalling