Job ID = 14168094 SRX = SRX8723976 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 46067027 spots for SRR12213374/SRR12213374.sra Written 46067027 spots for SRR12213374/SRR12213374.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168892 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:09 46067027 reads; of these: 46067027 (100.00%) were unpaired; of these: 2019899 (4.38%) aligned 0 times 32251645 (70.01%) aligned exactly 1 time 11795483 (25.61%) aligned >1 times 95.62% overall alignment rate Time searching: 00:12:09 Overall time: 00:12:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11170202 / 44047128 = 0.2536 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:43:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:43:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:43:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:43:50: 1000000 INFO @ Fri, 10 Dec 2021 15:43:56: 2000000 INFO @ Fri, 10 Dec 2021 15:44:02: 3000000 INFO @ Fri, 10 Dec 2021 15:44:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:44:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:44:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:44:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:44:15: 5000000 INFO @ Fri, 10 Dec 2021 15:44:21: 1000000 INFO @ Fri, 10 Dec 2021 15:44:21: 6000000 INFO @ Fri, 10 Dec 2021 15:44:27: 2000000 INFO @ Fri, 10 Dec 2021 15:44:27: 7000000 INFO @ Fri, 10 Dec 2021 15:44:34: 3000000 INFO @ Fri, 10 Dec 2021 15:44:34: 8000000 INFO @ Fri, 10 Dec 2021 15:44:40: 4000000 INFO @ Fri, 10 Dec 2021 15:44:41: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:44:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:44:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:44:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:44:47: 5000000 INFO @ Fri, 10 Dec 2021 15:44:47: 10000000 INFO @ Fri, 10 Dec 2021 15:44:51: 1000000 INFO @ Fri, 10 Dec 2021 15:44:53: 6000000 INFO @ Fri, 10 Dec 2021 15:44:54: 11000000 INFO @ Fri, 10 Dec 2021 15:44:58: 2000000 INFO @ Fri, 10 Dec 2021 15:45:00: 12000000 INFO @ Fri, 10 Dec 2021 15:45:00: 7000000 INFO @ Fri, 10 Dec 2021 15:45:05: 3000000 INFO @ Fri, 10 Dec 2021 15:45:07: 8000000 INFO @ Fri, 10 Dec 2021 15:45:07: 13000000 INFO @ Fri, 10 Dec 2021 15:45:11: 4000000 INFO @ Fri, 10 Dec 2021 15:45:14: 9000000 INFO @ Fri, 10 Dec 2021 15:45:14: 14000000 INFO @ Fri, 10 Dec 2021 15:45:18: 5000000 INFO @ Fri, 10 Dec 2021 15:45:20: 10000000 INFO @ Fri, 10 Dec 2021 15:45:20: 15000000 INFO @ Fri, 10 Dec 2021 15:45:25: 6000000 INFO @ Fri, 10 Dec 2021 15:45:27: 11000000 INFO @ Fri, 10 Dec 2021 15:45:27: 16000000 INFO @ Fri, 10 Dec 2021 15:45:31: 7000000 INFO @ Fri, 10 Dec 2021 15:45:33: 12000000 INFO @ Fri, 10 Dec 2021 15:45:33: 17000000 INFO @ Fri, 10 Dec 2021 15:45:38: 8000000 INFO @ Fri, 10 Dec 2021 15:45:40: 13000000 INFO @ Fri, 10 Dec 2021 15:45:40: 18000000 INFO @ Fri, 10 Dec 2021 15:45:44: 9000000 INFO @ Fri, 10 Dec 2021 15:45:46: 14000000 INFO @ Fri, 10 Dec 2021 15:45:47: 19000000 INFO @ Fri, 10 Dec 2021 15:45:51: 10000000 INFO @ Fri, 10 Dec 2021 15:45:53: 15000000 INFO @ Fri, 10 Dec 2021 15:45:53: 20000000 INFO @ Fri, 10 Dec 2021 15:45:58: 11000000 INFO @ Fri, 10 Dec 2021 15:45:59: 16000000 INFO @ Fri, 10 Dec 2021 15:46:00: 21000000 INFO @ Fri, 10 Dec 2021 15:46:04: 12000000 INFO @ Fri, 10 Dec 2021 15:46:06: 17000000 INFO @ Fri, 10 Dec 2021 15:46:07: 22000000 INFO @ Fri, 10 Dec 2021 15:46:12: 13000000 INFO @ Fri, 10 Dec 2021 15:46:13: 18000000 INFO @ Fri, 10 Dec 2021 15:46:14: 23000000 INFO @ Fri, 10 Dec 2021 15:46:19: 14000000 INFO @ Fri, 10 Dec 2021 15:46:20: 19000000 INFO @ Fri, 10 Dec 2021 15:46:21: 24000000 INFO @ Fri, 10 Dec 2021 15:46:25: 15000000 INFO @ Fri, 10 Dec 2021 15:46:26: 20000000 INFO @ Fri, 10 Dec 2021 15:46:28: 25000000 INFO @ Fri, 10 Dec 2021 15:46:32: 16000000 INFO @ Fri, 10 Dec 2021 15:46:33: 21000000 INFO @ Fri, 10 Dec 2021 15:46:34: 26000000 INFO @ Fri, 10 Dec 2021 15:46:38: 17000000 INFO @ Fri, 10 Dec 2021 15:46:40: 22000000 INFO @ Fri, 10 Dec 2021 15:46:40: 27000000 INFO @ Fri, 10 Dec 2021 15:46:44: 18000000 INFO @ Fri, 10 Dec 2021 15:46:47: 28000000 INFO @ Fri, 10 Dec 2021 15:46:47: 23000000 INFO @ Fri, 10 Dec 2021 15:46:51: 19000000 INFO @ Fri, 10 Dec 2021 15:46:53: 29000000 INFO @ Fri, 10 Dec 2021 15:46:54: 24000000 INFO @ Fri, 10 Dec 2021 15:46:59: 20000000 INFO @ Fri, 10 Dec 2021 15:47:00: 30000000 INFO @ Fri, 10 Dec 2021 15:47:00: 25000000 INFO @ Fri, 10 Dec 2021 15:47:05: 21000000 INFO @ Fri, 10 Dec 2021 15:47:06: 31000000 INFO @ Fri, 10 Dec 2021 15:47:07: 26000000 INFO @ Fri, 10 Dec 2021 15:47:12: 22000000 INFO @ Fri, 10 Dec 2021 15:47:13: 32000000 INFO @ Fri, 10 Dec 2021 15:47:13: 27000000 INFO @ Fri, 10 Dec 2021 15:47:18: 23000000 INFO @ Fri, 10 Dec 2021 15:47:19: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:47:19: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:47:19: #1 total tags in treatment: 32876926 INFO @ Fri, 10 Dec 2021 15:47:19: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:47:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:47:19: 28000000 INFO @ Fri, 10 Dec 2021 15:47:19: #1 tags after filtering in treatment: 32876926 INFO @ Fri, 10 Dec 2021 15:47:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:47:19: #1 finished! INFO @ Fri, 10 Dec 2021 15:47:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:47:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:47:21: #2 number of paired peaks: 74 WARNING @ Fri, 10 Dec 2021 15:47:21: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:47:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:47:24: 24000000 INFO @ Fri, 10 Dec 2021 15:47:25: 29000000 INFO @ Fri, 10 Dec 2021 15:47:30: 25000000 INFO @ Fri, 10 Dec 2021 15:47:31: 30000000 INFO @ Fri, 10 Dec 2021 15:47:37: 26000000 INFO @ Fri, 10 Dec 2021 15:47:38: 31000000 INFO @ Fri, 10 Dec 2021 15:47:43: 27000000 INFO @ Fri, 10 Dec 2021 15:47:44: 32000000 INFO @ Fri, 10 Dec 2021 15:47:49: 28000000 INFO @ Fri, 10 Dec 2021 15:47:50: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:47:50: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:47:50: #1 total tags in treatment: 32876926 INFO @ Fri, 10 Dec 2021 15:47:50: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:47:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:47:50: #1 tags after filtering in treatment: 32876926 INFO @ Fri, 10 Dec 2021 15:47:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:47:50: #1 finished! INFO @ Fri, 10 Dec 2021 15:47:50: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:47:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:47:53: #2 number of paired peaks: 74 WARNING @ Fri, 10 Dec 2021 15:47:53: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:47:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:47:55: 29000000 INFO @ Fri, 10 Dec 2021 15:48:00: 30000000 INFO @ Fri, 10 Dec 2021 15:48:06: 31000000 INFO @ Fri, 10 Dec 2021 15:48:12: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 15:48:18: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:48:18: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:48:18: #1 total tags in treatment: 32876926 INFO @ Fri, 10 Dec 2021 15:48:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:48:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:48:19: #1 tags after filtering in treatment: 32876926 INFO @ Fri, 10 Dec 2021 15:48:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:48:19: #1 finished! INFO @ Fri, 10 Dec 2021 15:48:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:48:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:48:21: #2 number of paired peaks: 74 WARNING @ Fri, 10 Dec 2021 15:48:21: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723976/SRX8723976.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。