Job ID = 14168093 SRX = SRX8723975 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 50702107 spots for SRR12213375/SRR12213375.sra Written 50702107 spots for SRR12213375/SRR12213375.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168888 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:11:35 50702107 reads; of these: 50702107 (100.00%) were unpaired; of these: 2145252 (4.23%) aligned 0 times 36340137 (71.67%) aligned exactly 1 time 12216718 (24.10%) aligned >1 times 95.77% overall alignment rate Time searching: 00:11:36 Overall time: 00:11:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11882337 / 48556855 = 0.2447 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:40:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:40:41: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:40:41: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:40:47: 1000000 INFO @ Fri, 10 Dec 2021 15:40:54: 2000000 INFO @ Fri, 10 Dec 2021 15:41:01: 3000000 INFO @ Fri, 10 Dec 2021 15:41:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:41:11: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:41:11: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:41:13: 5000000 INFO @ Fri, 10 Dec 2021 15:41:17: 1000000 INFO @ Fri, 10 Dec 2021 15:41:20: 6000000 INFO @ Fri, 10 Dec 2021 15:41:23: 2000000 INFO @ Fri, 10 Dec 2021 15:41:27: 7000000 INFO @ Fri, 10 Dec 2021 15:41:29: 3000000 INFO @ Fri, 10 Dec 2021 15:41:33: 8000000 INFO @ Fri, 10 Dec 2021 15:41:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:41:40: 9000000 INFO @ Fri, 10 Dec 2021 15:41:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:41:41: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:41:41: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:41:41: 5000000 INFO @ Fri, 10 Dec 2021 15:41:47: 10000000 INFO @ Fri, 10 Dec 2021 15:41:47: 6000000 INFO @ Fri, 10 Dec 2021 15:41:48: 1000000 INFO @ Fri, 10 Dec 2021 15:41:54: 7000000 INFO @ Fri, 10 Dec 2021 15:41:54: 11000000 INFO @ Fri, 10 Dec 2021 15:41:55: 2000000 INFO @ Fri, 10 Dec 2021 15:42:00: 8000000 INFO @ Fri, 10 Dec 2021 15:42:01: 12000000 INFO @ Fri, 10 Dec 2021 15:42:02: 3000000 INFO @ Fri, 10 Dec 2021 15:42:06: 9000000 INFO @ Fri, 10 Dec 2021 15:42:07: 13000000 INFO @ Fri, 10 Dec 2021 15:42:09: 4000000 INFO @ Fri, 10 Dec 2021 15:42:12: 10000000 INFO @ Fri, 10 Dec 2021 15:42:14: 14000000 INFO @ Fri, 10 Dec 2021 15:42:16: 5000000 INFO @ Fri, 10 Dec 2021 15:42:18: 11000000 INFO @ Fri, 10 Dec 2021 15:42:21: 15000000 INFO @ Fri, 10 Dec 2021 15:42:23: 6000000 INFO @ Fri, 10 Dec 2021 15:42:24: 12000000 INFO @ Fri, 10 Dec 2021 15:42:28: 16000000 INFO @ Fri, 10 Dec 2021 15:42:30: 7000000 INFO @ Fri, 10 Dec 2021 15:42:30: 13000000 INFO @ Fri, 10 Dec 2021 15:42:34: 17000000 INFO @ Fri, 10 Dec 2021 15:42:36: 14000000 INFO @ Fri, 10 Dec 2021 15:42:37: 8000000 INFO @ Fri, 10 Dec 2021 15:42:41: 18000000 INFO @ Fri, 10 Dec 2021 15:42:42: 15000000 INFO @ Fri, 10 Dec 2021 15:42:43: 9000000 INFO @ Fri, 10 Dec 2021 15:42:48: 19000000 INFO @ Fri, 10 Dec 2021 15:42:48: 16000000 INFO @ Fri, 10 Dec 2021 15:42:50: 10000000 INFO @ Fri, 10 Dec 2021 15:42:54: 17000000 INFO @ Fri, 10 Dec 2021 15:42:55: 20000000 INFO @ Fri, 10 Dec 2021 15:42:57: 11000000 INFO @ Fri, 10 Dec 2021 15:43:00: 18000000 INFO @ Fri, 10 Dec 2021 15:43:01: 21000000 INFO @ Fri, 10 Dec 2021 15:43:04: 12000000 INFO @ Fri, 10 Dec 2021 15:43:07: 19000000 INFO @ Fri, 10 Dec 2021 15:43:08: 22000000 INFO @ Fri, 10 Dec 2021 15:43:11: 13000000 INFO @ Fri, 10 Dec 2021 15:43:13: 20000000 INFO @ Fri, 10 Dec 2021 15:43:15: 23000000 INFO @ Fri, 10 Dec 2021 15:43:17: 14000000 INFO @ Fri, 10 Dec 2021 15:43:19: 21000000 INFO @ Fri, 10 Dec 2021 15:43:22: 24000000 INFO @ Fri, 10 Dec 2021 15:43:24: 15000000 INFO @ Fri, 10 Dec 2021 15:43:25: 22000000 INFO @ Fri, 10 Dec 2021 15:43:29: 25000000 INFO @ Fri, 10 Dec 2021 15:43:31: 23000000 INFO @ Fri, 10 Dec 2021 15:43:31: 16000000 INFO @ Fri, 10 Dec 2021 15:43:35: 26000000 INFO @ Fri, 10 Dec 2021 15:43:37: 24000000 INFO @ Fri, 10 Dec 2021 15:43:38: 17000000 INFO @ Fri, 10 Dec 2021 15:43:42: 27000000 INFO @ Fri, 10 Dec 2021 15:43:43: 25000000 INFO @ Fri, 10 Dec 2021 15:43:45: 18000000 INFO @ Fri, 10 Dec 2021 15:43:49: 26000000 INFO @ Fri, 10 Dec 2021 15:43:49: 28000000 INFO @ Fri, 10 Dec 2021 15:43:51: 19000000 INFO @ Fri, 10 Dec 2021 15:43:55: 27000000 INFO @ Fri, 10 Dec 2021 15:43:56: 29000000 INFO @ Fri, 10 Dec 2021 15:43:58: 20000000 INFO @ Fri, 10 Dec 2021 15:44:01: 28000000 INFO @ Fri, 10 Dec 2021 15:44:03: 30000000 INFO @ Fri, 10 Dec 2021 15:44:05: 21000000 INFO @ Fri, 10 Dec 2021 15:44:08: 29000000 INFO @ Fri, 10 Dec 2021 15:44:10: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 15:44:12: 22000000 INFO @ Fri, 10 Dec 2021 15:44:14: 30000000 INFO @ Fri, 10 Dec 2021 15:44:17: 32000000 INFO @ Fri, 10 Dec 2021 15:44:18: 23000000 INFO @ Fri, 10 Dec 2021 15:44:20: 31000000 INFO @ Fri, 10 Dec 2021 15:44:24: 33000000 INFO @ Fri, 10 Dec 2021 15:44:25: 24000000 INFO @ Fri, 10 Dec 2021 15:44:26: 32000000 INFO @ Fri, 10 Dec 2021 15:44:30: 34000000 INFO @ Fri, 10 Dec 2021 15:44:32: 33000000 INFO @ Fri, 10 Dec 2021 15:44:32: 25000000 INFO @ Fri, 10 Dec 2021 15:44:37: 35000000 INFO @ Fri, 10 Dec 2021 15:44:38: 34000000 INFO @ Fri, 10 Dec 2021 15:44:39: 26000000 INFO @ Fri, 10 Dec 2021 15:44:44: 35000000 INFO @ Fri, 10 Dec 2021 15:44:45: 36000000 INFO @ Fri, 10 Dec 2021 15:44:46: 27000000 INFO @ Fri, 10 Dec 2021 15:44:49: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:44:49: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:44:49: #1 total tags in treatment: 36674518 INFO @ Fri, 10 Dec 2021 15:44:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:44:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:44:50: #1 tags after filtering in treatment: 36674518 INFO @ Fri, 10 Dec 2021 15:44:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:44:50: #1 finished! INFO @ Fri, 10 Dec 2021 15:44:50: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:44:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:44:50: 36000000 INFO @ Fri, 10 Dec 2021 15:44:52: #2 number of paired peaks: 66 WARNING @ Fri, 10 Dec 2021 15:44:52: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:44:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:44:53: 28000000 INFO @ Fri, 10 Dec 2021 15:44:54: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:44:54: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:44:54: #1 total tags in treatment: 36674518 INFO @ Fri, 10 Dec 2021 15:44:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:44:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:44:55: #1 tags after filtering in treatment: 36674518 INFO @ Fri, 10 Dec 2021 15:44:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:44:55: #1 finished! INFO @ Fri, 10 Dec 2021 15:44:55: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:44:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:44:57: #2 number of paired peaks: 66 WARNING @ Fri, 10 Dec 2021 15:44:57: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:44:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:44:59: 29000000 INFO @ Fri, 10 Dec 2021 15:45:06: 30000000 INFO @ Fri, 10 Dec 2021 15:45:12: 31000000 INFO @ Fri, 10 Dec 2021 15:45:19: 32000000 INFO @ Fri, 10 Dec 2021 15:45:25: 33000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 15:45:31: 34000000 INFO @ Fri, 10 Dec 2021 15:45:38: 35000000 INFO @ Fri, 10 Dec 2021 15:45:44: 36000000 INFO @ Fri, 10 Dec 2021 15:45:49: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 15:45:49: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 15:45:49: #1 total tags in treatment: 36674518 INFO @ Fri, 10 Dec 2021 15:45:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:45:50: #1 tags after filtering in treatment: 36674518 INFO @ Fri, 10 Dec 2021 15:45:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 15:45:50: #1 finished! INFO @ Fri, 10 Dec 2021 15:45:50: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:45:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:45:52: #2 number of paired peaks: 66 WARNING @ Fri, 10 Dec 2021 15:45:52: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 15:45:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8723975/SRX8723975.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling