Job ID = 14167042 SRX = SRX8694699 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 38737751 spots for SRR12180109/SRR12180109.sra Written 38737751 spots for SRR12180109/SRR12180109.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167922 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:28:03 38737751 reads; of these: 38737751 (100.00%) were paired; of these: 14452327 (37.31%) aligned concordantly 0 times 17097155 (44.14%) aligned concordantly exactly 1 time 7188269 (18.56%) aligned concordantly >1 times ---- 14452327 pairs aligned concordantly 0 times; of these: 3988963 (27.60%) aligned discordantly 1 time ---- 10463364 pairs aligned 0 times concordantly or discordantly; of these: 20926728 mates make up the pairs; of these: 11910804 (56.92%) aligned 0 times 4230394 (20.22%) aligned exactly 1 time 4785530 (22.87%) aligned >1 times 84.63% overall alignment rate Time searching: 02:28:03 Overall time: 02:28:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3188607 / 26890048 = 0.1186 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:10:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:10:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:10:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:10:34: 1000000 INFO @ Fri, 10 Dec 2021 12:10:41: 2000000 INFO @ Fri, 10 Dec 2021 12:10:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:10:55: 4000000 INFO @ Fri, 10 Dec 2021 12:10:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:10:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:10:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:11:03: 5000000 INFO @ Fri, 10 Dec 2021 12:11:05: 1000000 INFO @ Fri, 10 Dec 2021 12:11:11: 6000000 INFO @ Fri, 10 Dec 2021 12:11:14: 2000000 INFO @ Fri, 10 Dec 2021 12:11:19: 7000000 INFO @ Fri, 10 Dec 2021 12:11:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:11:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:11:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:11:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:11:27: 8000000 INFO @ Fri, 10 Dec 2021 12:11:31: 4000000 INFO @ Fri, 10 Dec 2021 12:11:35: 9000000 INFO @ Fri, 10 Dec 2021 12:11:35: 1000000 INFO @ Fri, 10 Dec 2021 12:11:40: 5000000 INFO @ Fri, 10 Dec 2021 12:11:43: 10000000 INFO @ Fri, 10 Dec 2021 12:11:44: 2000000 INFO @ Fri, 10 Dec 2021 12:11:49: 6000000 INFO @ Fri, 10 Dec 2021 12:11:50: 11000000 INFO @ Fri, 10 Dec 2021 12:11:53: 3000000 INFO @ Fri, 10 Dec 2021 12:11:58: 12000000 INFO @ Fri, 10 Dec 2021 12:11:58: 7000000 INFO @ Fri, 10 Dec 2021 12:12:02: 4000000 INFO @ Fri, 10 Dec 2021 12:12:06: 13000000 INFO @ Fri, 10 Dec 2021 12:12:07: 8000000 INFO @ Fri, 10 Dec 2021 12:12:11: 5000000 INFO @ Fri, 10 Dec 2021 12:12:14: 14000000 INFO @ Fri, 10 Dec 2021 12:12:16: 9000000 INFO @ Fri, 10 Dec 2021 12:12:20: 6000000 INFO @ Fri, 10 Dec 2021 12:12:22: 15000000 INFO @ Fri, 10 Dec 2021 12:12:25: 10000000 INFO @ Fri, 10 Dec 2021 12:12:29: 7000000 INFO @ Fri, 10 Dec 2021 12:12:29: 16000000 INFO @ Fri, 10 Dec 2021 12:12:34: 11000000 INFO @ Fri, 10 Dec 2021 12:12:37: 17000000 INFO @ Fri, 10 Dec 2021 12:12:38: 8000000 INFO @ Fri, 10 Dec 2021 12:12:43: 12000000 INFO @ Fri, 10 Dec 2021 12:12:45: 18000000 INFO @ Fri, 10 Dec 2021 12:12:47: 9000000 INFO @ Fri, 10 Dec 2021 12:12:52: 13000000 INFO @ Fri, 10 Dec 2021 12:12:52: 19000000 INFO @ Fri, 10 Dec 2021 12:12:56: 10000000 INFO @ Fri, 10 Dec 2021 12:13:00: 20000000 INFO @ Fri, 10 Dec 2021 12:13:01: 14000000 INFO @ Fri, 10 Dec 2021 12:13:05: 11000000 INFO @ Fri, 10 Dec 2021 12:13:08: 21000000 INFO @ Fri, 10 Dec 2021 12:13:10: 15000000 INFO @ Fri, 10 Dec 2021 12:13:13: 12000000 INFO @ Fri, 10 Dec 2021 12:13:15: 22000000 INFO @ Fri, 10 Dec 2021 12:13:19: 16000000 INFO @ Fri, 10 Dec 2021 12:13:22: 13000000 INFO @ Fri, 10 Dec 2021 12:13:23: 23000000 INFO @ Fri, 10 Dec 2021 12:13:28: 17000000 INFO @ Fri, 10 Dec 2021 12:13:30: 24000000 INFO @ Fri, 10 Dec 2021 12:13:31: 14000000 INFO @ Fri, 10 Dec 2021 12:13:37: 18000000 INFO @ Fri, 10 Dec 2021 12:13:38: 25000000 INFO @ Fri, 10 Dec 2021 12:13:40: 15000000 INFO @ Fri, 10 Dec 2021 12:13:45: 26000000 INFO @ Fri, 10 Dec 2021 12:13:46: 19000000 INFO @ Fri, 10 Dec 2021 12:13:49: 16000000 INFO @ Fri, 10 Dec 2021 12:13:53: 27000000 INFO @ Fri, 10 Dec 2021 12:13:55: 20000000 INFO @ Fri, 10 Dec 2021 12:13:58: 17000000 INFO @ Fri, 10 Dec 2021 12:14:00: 28000000 INFO @ Fri, 10 Dec 2021 12:14:04: 21000000 INFO @ Fri, 10 Dec 2021 12:14:08: 18000000 INFO @ Fri, 10 Dec 2021 12:14:08: 29000000 INFO @ Fri, 10 Dec 2021 12:14:14: 22000000 INFO @ Fri, 10 Dec 2021 12:14:15: 30000000 INFO @ Fri, 10 Dec 2021 12:14:17: 19000000 INFO @ Fri, 10 Dec 2021 12:14:22: 31000000 INFO @ Fri, 10 Dec 2021 12:14:23: 23000000 INFO @ Fri, 10 Dec 2021 12:14:26: 20000000 INFO @ Fri, 10 Dec 2021 12:14:30: 32000000 INFO @ Fri, 10 Dec 2021 12:14:32: 24000000 INFO @ Fri, 10 Dec 2021 12:14:35: 21000000 INFO @ Fri, 10 Dec 2021 12:14:38: 33000000 INFO @ Fri, 10 Dec 2021 12:14:42: 25000000 INFO @ Fri, 10 Dec 2021 12:14:44: 22000000 INFO @ Fri, 10 Dec 2021 12:14:45: 34000000 INFO @ Fri, 10 Dec 2021 12:14:51: 26000000 INFO @ Fri, 10 Dec 2021 12:14:52: 35000000 INFO @ Fri, 10 Dec 2021 12:14:53: 23000000 INFO @ Fri, 10 Dec 2021 12:15:00: 36000000 INFO @ Fri, 10 Dec 2021 12:15:00: 27000000 INFO @ Fri, 10 Dec 2021 12:15:02: 24000000 INFO @ Fri, 10 Dec 2021 12:15:07: 37000000 INFO @ Fri, 10 Dec 2021 12:15:09: 28000000 INFO @ Fri, 10 Dec 2021 12:15:12: 25000000 INFO @ Fri, 10 Dec 2021 12:15:14: 38000000 INFO @ Fri, 10 Dec 2021 12:15:18: 29000000 INFO @ Fri, 10 Dec 2021 12:15:21: 26000000 INFO @ Fri, 10 Dec 2021 12:15:22: 39000000 INFO @ Fri, 10 Dec 2021 12:15:27: 30000000 INFO @ Fri, 10 Dec 2021 12:15:29: 27000000 INFO @ Fri, 10 Dec 2021 12:15:29: 40000000 INFO @ Fri, 10 Dec 2021 12:15:36: 31000000 INFO @ Fri, 10 Dec 2021 12:15:37: 41000000 INFO @ Fri, 10 Dec 2021 12:15:38: 28000000 INFO @ Fri, 10 Dec 2021 12:15:44: 42000000 INFO @ Fri, 10 Dec 2021 12:15:44: 32000000 INFO @ Fri, 10 Dec 2021 12:15:46: 29000000 INFO @ Fri, 10 Dec 2021 12:15:52: 43000000 INFO @ Fri, 10 Dec 2021 12:15:53: 33000000 INFO @ Fri, 10 Dec 2021 12:15:55: 30000000 INFO @ Fri, 10 Dec 2021 12:15:59: 44000000 INFO @ Fri, 10 Dec 2021 12:16:02: 34000000 INFO @ Fri, 10 Dec 2021 12:16:04: 31000000 INFO @ Fri, 10 Dec 2021 12:16:07: 45000000 INFO @ Fri, 10 Dec 2021 12:16:10: 35000000 INFO @ Fri, 10 Dec 2021 12:16:12: 32000000 INFO @ Fri, 10 Dec 2021 12:16:14: 46000000 INFO @ Fri, 10 Dec 2021 12:16:19: 36000000 INFO @ Fri, 10 Dec 2021 12:16:21: 33000000 INFO @ Fri, 10 Dec 2021 12:16:22: 47000000 INFO @ Fri, 10 Dec 2021 12:16:28: 37000000 INFO @ Fri, 10 Dec 2021 12:16:30: 48000000 INFO @ Fri, 10 Dec 2021 12:16:30: 34000000 INFO @ Fri, 10 Dec 2021 12:16:36: 38000000 INFO @ Fri, 10 Dec 2021 12:16:37: 49000000 INFO @ Fri, 10 Dec 2021 12:16:38: 35000000 INFO @ Fri, 10 Dec 2021 12:16:44: 50000000 INFO @ Fri, 10 Dec 2021 12:16:45: 39000000 INFO @ Fri, 10 Dec 2021 12:16:46: 36000000 INFO @ Fri, 10 Dec 2021 12:16:52: 51000000 INFO @ Fri, 10 Dec 2021 12:16:54: 40000000 INFO @ Fri, 10 Dec 2021 12:16:55: 37000000 INFO @ Fri, 10 Dec 2021 12:16:59: 52000000 INFO @ Fri, 10 Dec 2021 12:17:02: 41000000 INFO @ Fri, 10 Dec 2021 12:17:04: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:17:06: 53000000 INFO @ Fri, 10 Dec 2021 12:17:11: 42000000 INFO @ Fri, 10 Dec 2021 12:17:12: 39000000 INFO @ Fri, 10 Dec 2021 12:17:14: 54000000 INFO @ Fri, 10 Dec 2021 12:17:20: 43000000 INFO @ Fri, 10 Dec 2021 12:17:21: 55000000 INFO @ Fri, 10 Dec 2021 12:17:21: 40000000 INFO @ Fri, 10 Dec 2021 12:17:28: 56000000 INFO @ Fri, 10 Dec 2021 12:17:28: 44000000 INFO @ Fri, 10 Dec 2021 12:17:30: 41000000 INFO @ Fri, 10 Dec 2021 12:17:36: 57000000 INFO @ Fri, 10 Dec 2021 12:17:37: 45000000 INFO @ Fri, 10 Dec 2021 12:17:38: 42000000 INFO @ Fri, 10 Dec 2021 12:17:43: 58000000 INFO @ Fri, 10 Dec 2021 12:17:46: 46000000 INFO @ Fri, 10 Dec 2021 12:17:46: 43000000 INFO @ Fri, 10 Dec 2021 12:17:50: 59000000 INFO @ Fri, 10 Dec 2021 12:17:52: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 12:17:52: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 12:17:52: #1 total tags in treatment: 21366169 INFO @ Fri, 10 Dec 2021 12:17:52: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:17:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:17:52: #1 tags after filtering in treatment: 19019651 INFO @ Fri, 10 Dec 2021 12:17:52: #1 Redundant rate of treatment: 0.11 INFO @ Fri, 10 Dec 2021 12:17:52: #1 finished! INFO @ Fri, 10 Dec 2021 12:17:52: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:17:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:17:54: #2 number of paired peaks: 640 WARNING @ Fri, 10 Dec 2021 12:17:54: Fewer paired peaks (640) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 640 pairs to build model! INFO @ Fri, 10 Dec 2021 12:17:54: start model_add_line... INFO @ Fri, 10 Dec 2021 12:17:54: start X-correlation... INFO @ Fri, 10 Dec 2021 12:17:54: end of X-cor INFO @ Fri, 10 Dec 2021 12:17:54: #2 finished! INFO @ Fri, 10 Dec 2021 12:17:54: #2 predicted fragment length is 202 bps INFO @ Fri, 10 Dec 2021 12:17:54: #2 alternative fragment length(s) may be 202 bps INFO @ Fri, 10 Dec 2021 12:17:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.05_model.r WARNING @ Fri, 10 Dec 2021 12:17:54: #2 Since the d (202) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:17:54: #2 You may need to consider one of the other alternative d(s): 202 WARNING @ Fri, 10 Dec 2021 12:17:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:17:54: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:17:54: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:17:55: 47000000 INFO @ Fri, 10 Dec 2021 12:17:55: 44000000 INFO @ Fri, 10 Dec 2021 12:18:03: 48000000 INFO @ Fri, 10 Dec 2021 12:18:03: 45000000 INFO @ Fri, 10 Dec 2021 12:18:11: 49000000 INFO @ Fri, 10 Dec 2021 12:18:12: 46000000 INFO @ Fri, 10 Dec 2021 12:18:19: 50000000 INFO @ Fri, 10 Dec 2021 12:18:20: 47000000 INFO @ Fri, 10 Dec 2021 12:18:27: 51000000 INFO @ Fri, 10 Dec 2021 12:18:28: 48000000 INFO @ Fri, 10 Dec 2021 12:18:36: 52000000 INFO @ Fri, 10 Dec 2021 12:18:36: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:18:36: 49000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:18:44: 53000000 INFO @ Fri, 10 Dec 2021 12:18:44: 50000000 INFO @ Fri, 10 Dec 2021 12:18:52: 54000000 INFO @ Fri, 10 Dec 2021 12:18:52: 51000000 INFO @ Fri, 10 Dec 2021 12:18:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.05_peaks.xls INFO @ Fri, 10 Dec 2021 12:18:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:18:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.05_summits.bed INFO @ Fri, 10 Dec 2021 12:18:55: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3310 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:19:00: 55000000 INFO @ Fri, 10 Dec 2021 12:19:00: 52000000 INFO @ Fri, 10 Dec 2021 12:19:08: 56000000 INFO @ Fri, 10 Dec 2021 12:19:09: 53000000 INFO @ Fri, 10 Dec 2021 12:19:17: 57000000 INFO @ Fri, 10 Dec 2021 12:19:17: 54000000 INFO @ Fri, 10 Dec 2021 12:19:25: 55000000 INFO @ Fri, 10 Dec 2021 12:19:25: 58000000 INFO @ Fri, 10 Dec 2021 12:19:33: 56000000 INFO @ Fri, 10 Dec 2021 12:19:33: 59000000 INFO @ Fri, 10 Dec 2021 12:19:35: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 12:19:35: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 12:19:35: #1 total tags in treatment: 21366169 INFO @ Fri, 10 Dec 2021 12:19:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:19:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:19:36: #1 tags after filtering in treatment: 19019651 INFO @ Fri, 10 Dec 2021 12:19:36: #1 Redundant rate of treatment: 0.11 INFO @ Fri, 10 Dec 2021 12:19:36: #1 finished! INFO @ Fri, 10 Dec 2021 12:19:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:19:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:19:37: #2 number of paired peaks: 640 WARNING @ Fri, 10 Dec 2021 12:19:37: Fewer paired peaks (640) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 640 pairs to build model! INFO @ Fri, 10 Dec 2021 12:19:37: start model_add_line... INFO @ Fri, 10 Dec 2021 12:19:37: start X-correlation... INFO @ Fri, 10 Dec 2021 12:19:37: end of X-cor INFO @ Fri, 10 Dec 2021 12:19:37: #2 finished! INFO @ Fri, 10 Dec 2021 12:19:37: #2 predicted fragment length is 202 bps INFO @ Fri, 10 Dec 2021 12:19:37: #2 alternative fragment length(s) may be 202 bps INFO @ Fri, 10 Dec 2021 12:19:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.10_model.r WARNING @ Fri, 10 Dec 2021 12:19:37: #2 Since the d (202) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:19:37: #2 You may need to consider one of the other alternative d(s): 202 WARNING @ Fri, 10 Dec 2021 12:19:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:19:37: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:19:37: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:19:41: 57000000 INFO @ Fri, 10 Dec 2021 12:19:49: 58000000 INFO @ Fri, 10 Dec 2021 12:19:57: 59000000 INFO @ Fri, 10 Dec 2021 12:19:59: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 12:19:59: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 12:19:59: #1 total tags in treatment: 21366169 INFO @ Fri, 10 Dec 2021 12:19:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:19:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:19:59: #1 tags after filtering in treatment: 19019651 INFO @ Fri, 10 Dec 2021 12:19:59: #1 Redundant rate of treatment: 0.11 INFO @ Fri, 10 Dec 2021 12:19:59: #1 finished! INFO @ Fri, 10 Dec 2021 12:19:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:19:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:20:00: #2 number of paired peaks: 640 WARNING @ Fri, 10 Dec 2021 12:20:00: Fewer paired peaks (640) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 640 pairs to build model! INFO @ Fri, 10 Dec 2021 12:20:00: start model_add_line... INFO @ Fri, 10 Dec 2021 12:20:00: start X-correlation... INFO @ Fri, 10 Dec 2021 12:20:00: end of X-cor INFO @ Fri, 10 Dec 2021 12:20:00: #2 finished! INFO @ Fri, 10 Dec 2021 12:20:00: #2 predicted fragment length is 202 bps INFO @ Fri, 10 Dec 2021 12:20:00: #2 alternative fragment length(s) may be 202 bps INFO @ Fri, 10 Dec 2021 12:20:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.20_model.r WARNING @ Fri, 10 Dec 2021 12:20:00: #2 Since the d (202) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:20:00: #2 You may need to consider one of the other alternative d(s): 202 WARNING @ Fri, 10 Dec 2021 12:20:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:20:00: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:20:00: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:20:18: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:20:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.10_peaks.xls INFO @ Fri, 10 Dec 2021 12:20:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:20:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.10_summits.bed INFO @ Fri, 10 Dec 2021 12:20:36: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (2180 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:20:41: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:20:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.20_peaks.xls INFO @ Fri, 10 Dec 2021 12:20:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:20:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694699/SRX8694699.20_summits.bed INFO @ Fri, 10 Dec 2021 12:20:59: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1280 records, 4 fields): 9 millis CompletedMACS2peakCalling