Job ID = 14167037 SRX = SRX8694694 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 41736768 spots for SRR12180114/SRR12180114.sra Written 41736768 spots for SRR12180114/SRR12180114.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167793 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:06:12 41736768 reads; of these: 41736768 (100.00%) were paired; of these: 13892227 (33.29%) aligned concordantly 0 times 20316656 (48.68%) aligned concordantly exactly 1 time 7527885 (18.04%) aligned concordantly >1 times ---- 13892227 pairs aligned concordantly 0 times; of these: 3199823 (23.03%) aligned discordantly 1 time ---- 10692404 pairs aligned 0 times concordantly or discordantly; of these: 21384808 mates make up the pairs; of these: 16927449 (79.16%) aligned 0 times 1532069 (7.16%) aligned exactly 1 time 2925290 (13.68%) aligned >1 times 79.72% overall alignment rate Time searching: 02:06:13 Overall time: 02:06:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6653289 / 30759338 = 0.2163 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:38:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:38:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:38:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:38:42: 1000000 INFO @ Fri, 10 Dec 2021 11:38:49: 2000000 INFO @ Fri, 10 Dec 2021 11:38:55: 3000000 INFO @ Fri, 10 Dec 2021 11:39:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:39:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:39:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:39:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:39:09: 5000000 INFO @ Fri, 10 Dec 2021 11:39:15: 1000000 INFO @ Fri, 10 Dec 2021 11:39:16: 6000000 INFO @ Fri, 10 Dec 2021 11:39:24: 7000000 INFO @ Fri, 10 Dec 2021 11:39:24: 2000000 INFO @ Fri, 10 Dec 2021 11:39:31: 8000000 INFO @ Fri, 10 Dec 2021 11:39:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:39:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:39:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:39:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:39:38: 9000000 INFO @ Fri, 10 Dec 2021 11:39:42: 4000000 INFO @ Fri, 10 Dec 2021 11:39:43: 1000000 INFO @ Fri, 10 Dec 2021 11:39:46: 10000000 INFO @ Fri, 10 Dec 2021 11:39:51: 5000000 INFO @ Fri, 10 Dec 2021 11:39:51: 2000000 INFO @ Fri, 10 Dec 2021 11:39:54: 11000000 INFO @ Fri, 10 Dec 2021 11:39:58: 3000000 INFO @ Fri, 10 Dec 2021 11:40:00: 6000000 INFO @ Fri, 10 Dec 2021 11:40:01: 12000000 INFO @ Fri, 10 Dec 2021 11:40:06: 4000000 INFO @ Fri, 10 Dec 2021 11:40:09: 13000000 INFO @ Fri, 10 Dec 2021 11:40:09: 7000000 INFO @ Fri, 10 Dec 2021 11:40:13: 5000000 INFO @ Fri, 10 Dec 2021 11:40:16: 14000000 INFO @ Fri, 10 Dec 2021 11:40:18: 8000000 INFO @ Fri, 10 Dec 2021 11:40:21: 6000000 INFO @ Fri, 10 Dec 2021 11:40:24: 15000000 INFO @ Fri, 10 Dec 2021 11:40:27: 9000000 INFO @ Fri, 10 Dec 2021 11:40:28: 7000000 INFO @ Fri, 10 Dec 2021 11:40:31: 16000000 INFO @ Fri, 10 Dec 2021 11:40:36: 10000000 INFO @ Fri, 10 Dec 2021 11:40:36: 8000000 INFO @ Fri, 10 Dec 2021 11:40:39: 17000000 INFO @ Fri, 10 Dec 2021 11:40:43: 9000000 INFO @ Fri, 10 Dec 2021 11:40:45: 11000000 INFO @ Fri, 10 Dec 2021 11:40:46: 18000000 INFO @ Fri, 10 Dec 2021 11:40:51: 10000000 INFO @ Fri, 10 Dec 2021 11:40:53: 12000000 INFO @ Fri, 10 Dec 2021 11:40:54: 19000000 INFO @ Fri, 10 Dec 2021 11:40:58: 11000000 INFO @ Fri, 10 Dec 2021 11:41:01: 20000000 INFO @ Fri, 10 Dec 2021 11:41:02: 13000000 INFO @ Fri, 10 Dec 2021 11:41:06: 12000000 INFO @ Fri, 10 Dec 2021 11:41:09: 21000000 INFO @ Fri, 10 Dec 2021 11:41:12: 14000000 INFO @ Fri, 10 Dec 2021 11:41:13: 13000000 INFO @ Fri, 10 Dec 2021 11:41:16: 22000000 INFO @ Fri, 10 Dec 2021 11:41:21: 15000000 INFO @ Fri, 10 Dec 2021 11:41:21: 14000000 INFO @ Fri, 10 Dec 2021 11:41:24: 23000000 INFO @ Fri, 10 Dec 2021 11:41:28: 15000000 INFO @ Fri, 10 Dec 2021 11:41:30: 16000000 INFO @ Fri, 10 Dec 2021 11:41:31: 24000000 INFO @ Fri, 10 Dec 2021 11:41:36: 16000000 INFO @ Fri, 10 Dec 2021 11:41:39: 25000000 INFO @ Fri, 10 Dec 2021 11:41:39: 17000000 INFO @ Fri, 10 Dec 2021 11:41:43: 17000000 INFO @ Fri, 10 Dec 2021 11:41:46: 26000000 INFO @ Fri, 10 Dec 2021 11:41:48: 18000000 INFO @ Fri, 10 Dec 2021 11:41:51: 18000000 INFO @ Fri, 10 Dec 2021 11:41:54: 27000000 INFO @ Fri, 10 Dec 2021 11:41:57: 19000000 INFO @ Fri, 10 Dec 2021 11:41:59: 19000000 INFO @ Fri, 10 Dec 2021 11:42:02: 28000000 INFO @ Fri, 10 Dec 2021 11:42:06: 20000000 INFO @ Fri, 10 Dec 2021 11:42:07: 20000000 INFO @ Fri, 10 Dec 2021 11:42:09: 29000000 INFO @ Fri, 10 Dec 2021 11:42:15: 21000000 INFO @ Fri, 10 Dec 2021 11:42:16: 21000000 INFO @ Fri, 10 Dec 2021 11:42:17: 30000000 INFO @ Fri, 10 Dec 2021 11:42:24: 22000000 INFO @ Fri, 10 Dec 2021 11:42:24: 31000000 INFO @ Fri, 10 Dec 2021 11:42:25: 22000000 INFO @ Fri, 10 Dec 2021 11:42:31: 32000000 INFO @ Fri, 10 Dec 2021 11:42:33: 23000000 INFO @ Fri, 10 Dec 2021 11:42:33: 23000000 INFO @ Fri, 10 Dec 2021 11:42:39: 33000000 INFO @ Fri, 10 Dec 2021 11:42:42: 24000000 INFO @ Fri, 10 Dec 2021 11:42:42: 24000000 INFO @ Fri, 10 Dec 2021 11:42:47: 34000000 INFO @ Fri, 10 Dec 2021 11:42:51: 25000000 INFO @ Fri, 10 Dec 2021 11:42:52: 25000000 INFO @ Fri, 10 Dec 2021 11:42:54: 35000000 INFO @ Fri, 10 Dec 2021 11:43:00: 26000000 INFO @ Fri, 10 Dec 2021 11:43:01: 26000000 INFO @ Fri, 10 Dec 2021 11:43:02: 36000000 INFO @ Fri, 10 Dec 2021 11:43:09: 27000000 INFO @ Fri, 10 Dec 2021 11:43:10: 37000000 INFO @ Fri, 10 Dec 2021 11:43:10: 27000000 INFO @ Fri, 10 Dec 2021 11:43:17: 38000000 INFO @ Fri, 10 Dec 2021 11:43:19: 28000000 INFO @ Fri, 10 Dec 2021 11:43:19: 28000000 INFO @ Fri, 10 Dec 2021 11:43:24: 39000000 INFO @ Fri, 10 Dec 2021 11:43:28: 29000000 INFO @ Fri, 10 Dec 2021 11:43:29: 29000000 INFO @ Fri, 10 Dec 2021 11:43:32: 40000000 INFO @ Fri, 10 Dec 2021 11:43:37: 30000000 INFO @ Fri, 10 Dec 2021 11:43:38: 30000000 INFO @ Fri, 10 Dec 2021 11:43:40: 41000000 INFO @ Fri, 10 Dec 2021 11:43:46: 31000000 INFO @ Fri, 10 Dec 2021 11:43:47: 42000000 INFO @ Fri, 10 Dec 2021 11:43:47: 31000000 INFO @ Fri, 10 Dec 2021 11:43:54: 43000000 INFO @ Fri, 10 Dec 2021 11:43:55: 32000000 INFO @ Fri, 10 Dec 2021 11:43:56: 32000000 INFO @ Fri, 10 Dec 2021 11:44:02: 44000000 INFO @ Fri, 10 Dec 2021 11:44:04: 33000000 INFO @ Fri, 10 Dec 2021 11:44:05: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:44:09: 45000000 INFO @ Fri, 10 Dec 2021 11:44:14: 34000000 INFO @ Fri, 10 Dec 2021 11:44:14: 34000000 INFO @ Fri, 10 Dec 2021 11:44:17: 46000000 INFO @ Fri, 10 Dec 2021 11:44:23: 35000000 INFO @ Fri, 10 Dec 2021 11:44:24: 35000000 INFO @ Fri, 10 Dec 2021 11:44:24: 47000000 INFO @ Fri, 10 Dec 2021 11:44:32: 48000000 INFO @ Fri, 10 Dec 2021 11:44:32: 36000000 INFO @ Fri, 10 Dec 2021 11:44:33: 36000000 INFO @ Fri, 10 Dec 2021 11:44:39: 49000000 INFO @ Fri, 10 Dec 2021 11:44:41: 37000000 INFO @ Fri, 10 Dec 2021 11:44:42: 37000000 INFO @ Fri, 10 Dec 2021 11:44:47: 50000000 INFO @ Fri, 10 Dec 2021 11:44:50: 38000000 INFO @ Fri, 10 Dec 2021 11:44:51: 38000000 INFO @ Fri, 10 Dec 2021 11:44:54: 51000000 INFO @ Fri, 10 Dec 2021 11:44:59: 39000000 INFO @ Fri, 10 Dec 2021 11:45:00: 39000000 INFO @ Fri, 10 Dec 2021 11:45:02: 52000000 INFO @ Fri, 10 Dec 2021 11:45:08: 40000000 INFO @ Fri, 10 Dec 2021 11:45:08: 40000000 INFO @ Fri, 10 Dec 2021 11:45:09: 53000000 INFO @ Fri, 10 Dec 2021 11:45:11: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 11:45:11: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 11:45:11: #1 total tags in treatment: 21781346 INFO @ Fri, 10 Dec 2021 11:45:11: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:45:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:45:12: #1 tags after filtering in treatment: 19407458 INFO @ Fri, 10 Dec 2021 11:45:12: #1 Redundant rate of treatment: 0.11 INFO @ Fri, 10 Dec 2021 11:45:12: #1 finished! INFO @ Fri, 10 Dec 2021 11:45:12: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:45:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:45:13: #2 number of paired peaks: 747 WARNING @ Fri, 10 Dec 2021 11:45:13: Fewer paired peaks (747) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 747 pairs to build model! INFO @ Fri, 10 Dec 2021 11:45:13: start model_add_line... INFO @ Fri, 10 Dec 2021 11:45:13: start X-correlation... INFO @ Fri, 10 Dec 2021 11:45:13: end of X-cor INFO @ Fri, 10 Dec 2021 11:45:13: #2 finished! INFO @ Fri, 10 Dec 2021 11:45:13: #2 predicted fragment length is 217 bps INFO @ Fri, 10 Dec 2021 11:45:13: #2 alternative fragment length(s) may be 217 bps INFO @ Fri, 10 Dec 2021 11:45:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.05_model.r WARNING @ Fri, 10 Dec 2021 11:45:13: #2 Since the d (217) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 11:45:13: #2 You may need to consider one of the other alternative d(s): 217 WARNING @ Fri, 10 Dec 2021 11:45:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 11:45:13: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:45:13: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:45:17: 41000000 INFO @ Fri, 10 Dec 2021 11:45:17: 41000000 INFO @ Fri, 10 Dec 2021 11:45:25: 42000000 INFO @ Fri, 10 Dec 2021 11:45:26: 42000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:45:34: 43000000 INFO @ Fri, 10 Dec 2021 11:45:35: 43000000 INFO @ Fri, 10 Dec 2021 11:45:43: 44000000 INFO @ Fri, 10 Dec 2021 11:45:44: 44000000 INFO @ Fri, 10 Dec 2021 11:45:52: 45000000 INFO @ Fri, 10 Dec 2021 11:45:52: 45000000 INFO @ Fri, 10 Dec 2021 11:45:54: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:46:00: 46000000 INFO @ Fri, 10 Dec 2021 11:46:01: 46000000 INFO @ Fri, 10 Dec 2021 11:46:09: 47000000 INFO @ Fri, 10 Dec 2021 11:46:10: 47000000 INFO @ Fri, 10 Dec 2021 11:46:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.05_peaks.xls INFO @ Fri, 10 Dec 2021 11:46:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:46:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.05_summits.bed INFO @ Fri, 10 Dec 2021 11:46:12: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4095 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:46:18: 48000000 INFO @ Fri, 10 Dec 2021 11:46:19: 48000000 INFO @ Fri, 10 Dec 2021 11:46:28: 49000000 INFO @ Fri, 10 Dec 2021 11:46:29: 49000000 INFO @ Fri, 10 Dec 2021 11:46:37: 50000000 INFO @ Fri, 10 Dec 2021 11:46:38: 50000000 INFO @ Fri, 10 Dec 2021 11:46:46: 51000000 INFO @ Fri, 10 Dec 2021 11:46:46: 51000000 INFO @ Fri, 10 Dec 2021 11:46:55: 52000000 INFO @ Fri, 10 Dec 2021 11:46:56: 52000000 INFO @ Fri, 10 Dec 2021 11:47:04: 53000000 INFO @ Fri, 10 Dec 2021 11:47:05: 53000000 INFO @ Fri, 10 Dec 2021 11:47:06: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 11:47:06: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 11:47:06: #1 total tags in treatment: 21781346 INFO @ Fri, 10 Dec 2021 11:47:06: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:47:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:47:07: #1 tags after filtering in treatment: 19407458 INFO @ Fri, 10 Dec 2021 11:47:07: #1 Redundant rate of treatment: 0.11 INFO @ Fri, 10 Dec 2021 11:47:07: #1 finished! INFO @ Fri, 10 Dec 2021 11:47:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:47:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:47:07: #1 tag size is determined as 151 bps INFO @ Fri, 10 Dec 2021 11:47:07: #1 tag size = 151 INFO @ Fri, 10 Dec 2021 11:47:07: #1 total tags in treatment: 21781346 INFO @ Fri, 10 Dec 2021 11:47:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:47:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:47:07: #1 tags after filtering in treatment: 19407458 INFO @ Fri, 10 Dec 2021 11:47:07: #1 Redundant rate of treatment: 0.11 INFO @ Fri, 10 Dec 2021 11:47:07: #1 finished! INFO @ Fri, 10 Dec 2021 11:47:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:47:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:47:08: #2 number of paired peaks: 747 WARNING @ Fri, 10 Dec 2021 11:47:08: Fewer paired peaks (747) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 747 pairs to build model! INFO @ Fri, 10 Dec 2021 11:47:08: start model_add_line... INFO @ Fri, 10 Dec 2021 11:47:08: start X-correlation... INFO @ Fri, 10 Dec 2021 11:47:08: end of X-cor INFO @ Fri, 10 Dec 2021 11:47:08: #2 finished! INFO @ Fri, 10 Dec 2021 11:47:08: #2 predicted fragment length is 217 bps INFO @ Fri, 10 Dec 2021 11:47:08: #2 alternative fragment length(s) may be 217 bps INFO @ Fri, 10 Dec 2021 11:47:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.10_model.r WARNING @ Fri, 10 Dec 2021 11:47:08: #2 Since the d (217) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 11:47:08: #2 You may need to consider one of the other alternative d(s): 217 WARNING @ Fri, 10 Dec 2021 11:47:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 11:47:08: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:47:08: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:47:09: #2 number of paired peaks: 747 WARNING @ Fri, 10 Dec 2021 11:47:09: Fewer paired peaks (747) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 747 pairs to build model! INFO @ Fri, 10 Dec 2021 11:47:09: start model_add_line... INFO @ Fri, 10 Dec 2021 11:47:09: start X-correlation... INFO @ Fri, 10 Dec 2021 11:47:09: end of X-cor INFO @ Fri, 10 Dec 2021 11:47:09: #2 finished! INFO @ Fri, 10 Dec 2021 11:47:09: #2 predicted fragment length is 217 bps INFO @ Fri, 10 Dec 2021 11:47:09: #2 alternative fragment length(s) may be 217 bps INFO @ Fri, 10 Dec 2021 11:47:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.20_model.r WARNING @ Fri, 10 Dec 2021 11:47:09: #2 Since the d (217) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 11:47:09: #2 You may need to consider one of the other alternative d(s): 217 WARNING @ Fri, 10 Dec 2021 11:47:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 11:47:09: #3 Call peaks... INFO @ Fri, 10 Dec 2021 11:47:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 11:47:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:47:51: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 11:48:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.10_peaks.xls INFO @ Fri, 10 Dec 2021 11:48:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:48:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.10_summits.bed INFO @ Fri, 10 Dec 2021 11:48:08: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2778 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:48:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.20_peaks.xls INFO @ Fri, 10 Dec 2021 11:48:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 11:48:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8694694/SRX8694694.20_summits.bed INFO @ Fri, 10 Dec 2021 11:48:10: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1626 records, 4 fields): 3 millis CompletedMACS2peakCalling