Job ID = 14170319 SRX = SRX8689102 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 18410510 spots for SRR12174335/SRR12174335.sra Written 18410510 spots for SRR12174335/SRR12174335.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170751 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:03 18410510 reads; of these: 18410510 (100.00%) were unpaired; of these: 452911 (2.46%) aligned 0 times 13174951 (71.56%) aligned exactly 1 time 4782648 (25.98%) aligned >1 times 97.54% overall alignment rate Time searching: 00:06:03 Overall time: 00:06:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1446584 / 17957599 = 0.0806 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:29:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:29:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:29:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:29:10: 1000000 INFO @ Sat, 11 Dec 2021 06:29:16: 2000000 INFO @ Sat, 11 Dec 2021 06:29:21: 3000000 INFO @ Sat, 11 Dec 2021 06:29:27: 4000000 INFO @ Sat, 11 Dec 2021 06:29:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:29:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:29:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:29:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:29:38: 6000000 INFO @ Sat, 11 Dec 2021 06:29:41: 1000000 INFO @ Sat, 11 Dec 2021 06:29:44: 7000000 INFO @ Sat, 11 Dec 2021 06:29:47: 2000000 INFO @ Sat, 11 Dec 2021 06:29:50: 8000000 INFO @ Sat, 11 Dec 2021 06:29:53: 3000000 INFO @ Sat, 11 Dec 2021 06:29:56: 9000000 INFO @ Sat, 11 Dec 2021 06:30:00: 4000000 INFO @ Sat, 11 Dec 2021 06:30:02: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:30:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:30:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:30:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:30:06: 5000000 INFO @ Sat, 11 Dec 2021 06:30:08: 11000000 INFO @ Sat, 11 Dec 2021 06:30:11: 1000000 INFO @ Sat, 11 Dec 2021 06:30:12: 6000000 INFO @ Sat, 11 Dec 2021 06:30:14: 12000000 INFO @ Sat, 11 Dec 2021 06:30:17: 2000000 INFO @ Sat, 11 Dec 2021 06:30:18: 7000000 INFO @ Sat, 11 Dec 2021 06:30:20: 13000000 INFO @ Sat, 11 Dec 2021 06:30:24: 3000000 INFO @ Sat, 11 Dec 2021 06:30:24: 8000000 INFO @ Sat, 11 Dec 2021 06:30:26: 14000000 INFO @ Sat, 11 Dec 2021 06:30:30: 4000000 INFO @ Sat, 11 Dec 2021 06:30:30: 9000000 INFO @ Sat, 11 Dec 2021 06:30:32: 15000000 INFO @ Sat, 11 Dec 2021 06:30:36: 5000000 INFO @ Sat, 11 Dec 2021 06:30:37: 10000000 INFO @ Sat, 11 Dec 2021 06:30:38: 16000000 INFO @ Sat, 11 Dec 2021 06:30:42: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:30:42: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:30:42: #1 total tags in treatment: 16511015 INFO @ Sat, 11 Dec 2021 06:30:42: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:30:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:30:42: #1 tags after filtering in treatment: 16511015 INFO @ Sat, 11 Dec 2021 06:30:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:30:42: #1 finished! INFO @ Sat, 11 Dec 2021 06:30:42: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:30:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:30:42: 6000000 INFO @ Sat, 11 Dec 2021 06:30:43: 11000000 INFO @ Sat, 11 Dec 2021 06:30:43: #2 number of paired peaks: 95 WARNING @ Sat, 11 Dec 2021 06:30:43: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:30:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:30:48: 7000000 INFO @ Sat, 11 Dec 2021 06:30:49: 12000000 INFO @ Sat, 11 Dec 2021 06:30:54: 8000000 INFO @ Sat, 11 Dec 2021 06:30:55: 13000000 INFO @ Sat, 11 Dec 2021 06:31:00: 9000000 INFO @ Sat, 11 Dec 2021 06:31:01: 14000000 INFO @ Sat, 11 Dec 2021 06:31:06: 10000000 INFO @ Sat, 11 Dec 2021 06:31:07: 15000000 INFO @ Sat, 11 Dec 2021 06:31:12: 11000000 INFO @ Sat, 11 Dec 2021 06:31:13: 16000000 INFO @ Sat, 11 Dec 2021 06:31:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:31:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:31:16: #1 total tags in treatment: 16511015 INFO @ Sat, 11 Dec 2021 06:31:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:31:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:31:16: #1 tags after filtering in treatment: 16511015 INFO @ Sat, 11 Dec 2021 06:31:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:31:16: #1 finished! INFO @ Sat, 11 Dec 2021 06:31:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:31:16: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:31:18: #2 number of paired peaks: 95 WARNING @ Sat, 11 Dec 2021 06:31:18: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:31:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:31:18: 12000000 INFO @ Sat, 11 Dec 2021 06:31:23: 13000000 INFO @ Sat, 11 Dec 2021 06:31:29: 14000000 INFO @ Sat, 11 Dec 2021 06:31:34: 15000000 INFO @ Sat, 11 Dec 2021 06:31:40: 16000000 INFO @ Sat, 11 Dec 2021 06:31:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:31:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:31:43: #1 total tags in treatment: 16511015 INFO @ Sat, 11 Dec 2021 06:31:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:31:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:31:43: #1 tags after filtering in treatment: 16511015 INFO @ Sat, 11 Dec 2021 06:31:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:31:43: #1 finished! INFO @ Sat, 11 Dec 2021 06:31:43: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:31:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:31:44: #2 number of paired peaks: 95 WARNING @ Sat, 11 Dec 2021 06:31:44: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:31:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689102/SRX8689102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。