Job ID = 14170565 SRX = SRX8689097 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6378269 spots for SRR12174355/SRR12174355.sra Written 6378269 spots for SRR12174355/SRR12174355.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170969 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:42 6378269 reads; of these: 6378269 (100.00%) were unpaired; of these: 221377 (3.47%) aligned 0 times 5180783 (81.23%) aligned exactly 1 time 976109 (15.30%) aligned >1 times 96.53% overall alignment rate Time searching: 00:01:42 Overall time: 00:01:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 205319 / 6156892 = 0.0333 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:20:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:20:23: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:20:23: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:20:28: 1000000 INFO @ Sat, 11 Dec 2021 07:20:32: 2000000 INFO @ Sat, 11 Dec 2021 07:20:37: 3000000 INFO @ Sat, 11 Dec 2021 07:20:42: 4000000 INFO @ Sat, 11 Dec 2021 07:20:46: 5000000 INFO @ Sat, 11 Dec 2021 07:20:51: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:20:51: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:20:51: #1 total tags in treatment: 5951573 INFO @ Sat, 11 Dec 2021 07:20:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:20:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:20:51: #1 tags after filtering in treatment: 5951573 INFO @ Sat, 11 Dec 2021 07:20:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:20:51: #1 finished! INFO @ Sat, 11 Dec 2021 07:20:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:20:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:20:51: #2 number of paired peaks: 15 WARNING @ Sat, 11 Dec 2021 07:20:51: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:20:51: Process for pairing-model is terminated! WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:20:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:20:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:20:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:20:58: 1000000 INFO @ Sat, 11 Dec 2021 07:21:02: 2000000 INFO @ Sat, 11 Dec 2021 07:21:07: 3000000 INFO @ Sat, 11 Dec 2021 07:21:12: 4000000 INFO @ Sat, 11 Dec 2021 07:21:16: 5000000 INFO @ Sat, 11 Dec 2021 07:21:21: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:21:21: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:21:21: #1 total tags in treatment: 5951573 INFO @ Sat, 11 Dec 2021 07:21:21: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:21:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:21:21: #1 tags after filtering in treatment: 5951573 INFO @ Sat, 11 Dec 2021 07:21:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:21:21: #1 finished! INFO @ Sat, 11 Dec 2021 07:21:21: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:21:21: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... INFO @ Sat, 11 Dec 2021 07:21:21: #2 number of paired peaks: 15 WARNING @ Sat, 11 Dec 2021 07:21:21: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:21:21: Process for pairing-model is terminated! WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:21:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:21:23: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:21:23: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:21:28: 1000000 INFO @ Sat, 11 Dec 2021 07:21:33: 2000000 INFO @ Sat, 11 Dec 2021 07:21:38: 3000000 INFO @ Sat, 11 Dec 2021 07:21:42: 4000000 INFO @ Sat, 11 Dec 2021 07:21:47: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:21:52: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:21:52: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:21:52: #1 total tags in treatment: 5951573 INFO @ Sat, 11 Dec 2021 07:21:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:21:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:21:52: #1 tags after filtering in treatment: 5951573 INFO @ Sat, 11 Dec 2021 07:21:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:21:52: #1 finished! INFO @ Sat, 11 Dec 2021 07:21:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:21:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:21:53: #2 number of paired peaks: 15 WARNING @ Sat, 11 Dec 2021 07:21:53: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:21:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689097/SRX8689097.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。