Job ID = 14170510 SRX = SRX8689069 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6796177 spots for SRR12174204/SRR12174204.sra Written 6796177 spots for SRR12174204/SRR12174204.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170915 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:33 6796177 reads; of these: 6796177 (100.00%) were unpaired; of these: 335083 (4.93%) aligned 0 times 5718828 (84.15%) aligned exactly 1 time 742266 (10.92%) aligned >1 times 95.07% overall alignment rate Time searching: 00:01:33 Overall time: 00:01:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 288449 / 6461094 = 0.0446 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:05:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:05:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:05:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:05:09: 1000000 INFO @ Sat, 11 Dec 2021 07:05:15: 2000000 INFO @ Sat, 11 Dec 2021 07:05:21: 3000000 INFO @ Sat, 11 Dec 2021 07:05:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:05:32: 5000000 INFO @ Sat, 11 Dec 2021 07:05:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:05:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:05:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:05:38: 6000000 INFO @ Sat, 11 Dec 2021 07:05:39: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:05:39: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:05:39: #1 total tags in treatment: 6172645 INFO @ Sat, 11 Dec 2021 07:05:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:05:39: #1 tags after filtering in treatment: 6172645 INFO @ Sat, 11 Dec 2021 07:05:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:05:39: #1 finished! INFO @ Sat, 11 Dec 2021 07:05:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:05:39: #2 number of paired peaks: 7 WARNING @ Sat, 11 Dec 2021 07:05:39: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:05:39: Process for pairing-model is terminated! INFO @ Sat, 11 Dec 2021 07:05:40: 1000000 cut: /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:05:45: 2000000 INFO @ Sat, 11 Dec 2021 07:05:51: 3000000 INFO @ Sat, 11 Dec 2021 07:05:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:06:03: 5000000 INFO @ Sat, 11 Dec 2021 07:06:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:06:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:06:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:06:09: 6000000 INFO @ Sat, 11 Dec 2021 07:06:10: 1000000 INFO @ Sat, 11 Dec 2021 07:06:10: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:06:10: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:06:10: #1 total tags in treatment: 6172645 INFO @ Sat, 11 Dec 2021 07:06:10: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:06:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:06:10: #1 tags after filtering in treatment: 6172645 INFO @ Sat, 11 Dec 2021 07:06:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:06:10: #1 finished! INFO @ Sat, 11 Dec 2021 07:06:10: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:06:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:06:10: #2 number of paired peaks: 7 WARNING @ Sat, 11 Dec 2021 07:06:10: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:06:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:06:16: 2000000 INFO @ Sat, 11 Dec 2021 07:06:21: 3000000 INFO @ Sat, 11 Dec 2021 07:06:27: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:06:33: 5000000 INFO @ Sat, 11 Dec 2021 07:06:38: 6000000 INFO @ Sat, 11 Dec 2021 07:06:39: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:06:39: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:06:39: #1 total tags in treatment: 6172645 INFO @ Sat, 11 Dec 2021 07:06:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:06:39: #1 tags after filtering in treatment: 6172645 INFO @ Sat, 11 Dec 2021 07:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:06:39: #1 finished! INFO @ Sat, 11 Dec 2021 07:06:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:06:40: #2 number of paired peaks: 7 WARNING @ Sat, 11 Dec 2021 07:06:40: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:06:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689069/SRX8689069.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。