Job ID = 14170507 SRX = SRX8689066 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6567109 spots for SRR12174201/SRR12174201.sra Written 6567109 spots for SRR12174201/SRR12174201.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170914 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:00 6567109 reads; of these: 6567109 (100.00%) were unpaired; of these: 539679 (8.22%) aligned 0 times 5122725 (78.01%) aligned exactly 1 time 904705 (13.78%) aligned >1 times 91.78% overall alignment rate Time searching: 00:02:00 Overall time: 00:02:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 239683 / 6027430 = 0.0398 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:05:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:05:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:05:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:05:18: 1000000 INFO @ Sat, 11 Dec 2021 07:05:24: 2000000 INFO @ Sat, 11 Dec 2021 07:05:30: 3000000 INFO @ Sat, 11 Dec 2021 07:05:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:05:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:05:41: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:05:41: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:05:42: 5000000 INFO @ Sat, 11 Dec 2021 07:05:46: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:05:46: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:05:46: #1 total tags in treatment: 5787747 INFO @ Sat, 11 Dec 2021 07:05:46: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:05:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:05:47: #1 tags after filtering in treatment: 5787747 INFO @ Sat, 11 Dec 2021 07:05:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:05:47: #1 finished! INFO @ Sat, 11 Dec 2021 07:05:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:05:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:05:47: #2 number of paired peaks: 71 WARNING @ Sat, 11 Dec 2021 07:05:47: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:05:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:05:48: 1000000 INFO @ Sat, 11 Dec 2021 07:05:55: 2000000 INFO @ Sat, 11 Dec 2021 07:06:02: 3000000 INFO @ Sat, 11 Dec 2021 07:06:08: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:06:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:06:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:06:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:06:15: 5000000 INFO @ Sat, 11 Dec 2021 07:06:18: 1000000 INFO @ Sat, 11 Dec 2021 07:06:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:06:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:06:20: #1 total tags in treatment: 5787747 INFO @ Sat, 11 Dec 2021 07:06:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:06:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:06:20: #1 tags after filtering in treatment: 5787747 INFO @ Sat, 11 Dec 2021 07:06:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:06:20: #1 finished! INFO @ Sat, 11 Dec 2021 07:06:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:06:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:06:21: #2 number of paired peaks: 71 WARNING @ Sat, 11 Dec 2021 07:06:21: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:06:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:06:25: 2000000 INFO @ Sat, 11 Dec 2021 07:06:31: 3000000 INFO @ Sat, 11 Dec 2021 07:06:38: 4000000 INFO @ Sat, 11 Dec 2021 07:06:44: 5000000 INFO @ Sat, 11 Dec 2021 07:06:48: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:06:48: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:06:48: #1 total tags in treatment: 5787747 INFO @ Sat, 11 Dec 2021 07:06:48: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:06:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:06:48: #1 tags after filtering in treatment: 5787747 INFO @ Sat, 11 Dec 2021 07:06:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:06:48: #1 finished! INFO @ Sat, 11 Dec 2021 07:06:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:06:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:06:49: #2 number of paired peaks: 71 WARNING @ Sat, 11 Dec 2021 07:06:49: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:06:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689066/SRX8689066.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。