Job ID = 14170506 SRX = SRX8689065 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6566374 spots for SRR12174200/SRR12174200.sra Written 6566374 spots for SRR12174200/SRR12174200.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170911 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:51 6566374 reads; of these: 6566374 (100.00%) were unpaired; of these: 559222 (8.52%) aligned 0 times 5110679 (77.83%) aligned exactly 1 time 896473 (13.65%) aligned >1 times 91.48% overall alignment rate Time searching: 00:01:51 Overall time: 00:01:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 244151 / 6007152 = 0.0406 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:04:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:04:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:04:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:04:24: 1000000 INFO @ Sat, 11 Dec 2021 07:04:29: 2000000 INFO @ Sat, 11 Dec 2021 07:04:34: 3000000 INFO @ Sat, 11 Dec 2021 07:04:38: 4000000 INFO @ Sat, 11 Dec 2021 07:04:43: 5000000 INFO @ Sat, 11 Dec 2021 07:04:47: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:04:47: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:04:47: #1 total tags in treatment: 5763001 INFO @ Sat, 11 Dec 2021 07:04:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:04:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:04:47: #1 tags after filtering in treatment: 5763001 INFO @ Sat, 11 Dec 2021 07:04:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:04:47: #1 finished! INFO @ Sat, 11 Dec 2021 07:04:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:04:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:04:47: #2 number of paired peaks: 48 WARNING @ Sat, 11 Dec 2021 07:04:47: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:04:47: Process for pairing-model is terminated! WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:04:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:04:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:04:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:04:54: 1000000 INFO @ Sat, 11 Dec 2021 07:05:00: 2000000 INFO @ Sat, 11 Dec 2021 07:05:05: 3000000 INFO @ Sat, 11 Dec 2021 07:05:11: 4000000 INFO @ Sat, 11 Dec 2021 07:05:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:05:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:05:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:05:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:05:20: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:05:20: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:05:20: #1 total tags in treatment: 5763001 INFO @ Sat, 11 Dec 2021 07:05:20: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:05:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:05:20: #1 tags after filtering in treatment: 5763001 INFO @ Sat, 11 Dec 2021 07:05:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:05:20: #1 finished! INFO @ Sat, 11 Dec 2021 07:05:20: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:05:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:05:21: #2 number of paired peaks: 48 WARNING @ Sat, 11 Dec 2021 07:05:21: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:05:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:05:25: 1000000 INFO @ Sat, 11 Dec 2021 07:05:30: 2000000 INFO @ Sat, 11 Dec 2021 07:05:36: 3000000 INFO @ Sat, 11 Dec 2021 07:05:41: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:05:47: 5000000 INFO @ Sat, 11 Dec 2021 07:05:51: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:05:51: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:05:51: #1 total tags in treatment: 5763001 INFO @ Sat, 11 Dec 2021 07:05:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:05:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:05:51: #1 tags after filtering in treatment: 5763001 INFO @ Sat, 11 Dec 2021 07:05:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:05:51: #1 finished! INFO @ Sat, 11 Dec 2021 07:05:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:05:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:05:51: #2 number of paired peaks: 48 WARNING @ Sat, 11 Dec 2021 07:05:52: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:05:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689065/SRX8689065.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。