Job ID = 14170474 SRX = SRX8689039 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16670807 spots for SRR12174238/SRR12174238.sra Written 16670807 spots for SRR12174238/SRR12174238.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170896 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 16670807 reads; of these: 16670807 (100.00%) were unpaired; of these: 403653 (2.42%) aligned 0 times 11794911 (70.75%) aligned exactly 1 time 4472243 (26.83%) aligned >1 times 97.58% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1075480 / 16267154 = 0.0661 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:03:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:03:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:03:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:03:25: 1000000 INFO @ Sat, 11 Dec 2021 07:03:30: 2000000 INFO @ Sat, 11 Dec 2021 07:03:35: 3000000 INFO @ Sat, 11 Dec 2021 07:03:40: 4000000 INFO @ Sat, 11 Dec 2021 07:03:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:03:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:03:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:03:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:03:50: 6000000 INFO @ Sat, 11 Dec 2021 07:03:55: 1000000 INFO @ Sat, 11 Dec 2021 07:03:55: 7000000 INFO @ Sat, 11 Dec 2021 07:04:01: 2000000 INFO @ Sat, 11 Dec 2021 07:04:01: 8000000 INFO @ Sat, 11 Dec 2021 07:04:06: 3000000 INFO @ Sat, 11 Dec 2021 07:04:06: 9000000 INFO @ Sat, 11 Dec 2021 07:04:12: 10000000 INFO @ Sat, 11 Dec 2021 07:04:12: 4000000 INFO @ Sat, 11 Dec 2021 07:04:17: 11000000 INFO @ Sat, 11 Dec 2021 07:04:17: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:04:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:04:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:04:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:04:22: 12000000 INFO @ Sat, 11 Dec 2021 07:04:22: 6000000 INFO @ Sat, 11 Dec 2021 07:04:26: 1000000 INFO @ Sat, 11 Dec 2021 07:04:28: 13000000 INFO @ Sat, 11 Dec 2021 07:04:28: 7000000 INFO @ Sat, 11 Dec 2021 07:04:32: 2000000 INFO @ Sat, 11 Dec 2021 07:04:33: 14000000 INFO @ Sat, 11 Dec 2021 07:04:33: 8000000 INFO @ Sat, 11 Dec 2021 07:04:38: 3000000 INFO @ Sat, 11 Dec 2021 07:04:39: 15000000 INFO @ Sat, 11 Dec 2021 07:04:39: 9000000 INFO @ Sat, 11 Dec 2021 07:04:40: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:04:40: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:04:40: #1 total tags in treatment: 15191674 INFO @ Sat, 11 Dec 2021 07:04:40: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:04:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:04:40: #1 tags after filtering in treatment: 15191674 INFO @ Sat, 11 Dec 2021 07:04:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:04:40: #1 finished! INFO @ Sat, 11 Dec 2021 07:04:40: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:04:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:04:41: #2 number of paired peaks: 51 WARNING @ Sat, 11 Dec 2021 07:04:41: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:04:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:04:45: 4000000 INFO @ Sat, 11 Dec 2021 07:04:45: 10000000 INFO @ Sat, 11 Dec 2021 07:04:50: 11000000 INFO @ Sat, 11 Dec 2021 07:04:51: 5000000 INFO @ Sat, 11 Dec 2021 07:04:56: 12000000 INFO @ Sat, 11 Dec 2021 07:04:57: 6000000 INFO @ Sat, 11 Dec 2021 07:05:01: 13000000 INFO @ Sat, 11 Dec 2021 07:05:03: 7000000 INFO @ Sat, 11 Dec 2021 07:05:07: 14000000 INFO @ Sat, 11 Dec 2021 07:05:09: 8000000 INFO @ Sat, 11 Dec 2021 07:05:12: 15000000 INFO @ Sat, 11 Dec 2021 07:05:14: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:05:14: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:05:14: #1 total tags in treatment: 15191674 INFO @ Sat, 11 Dec 2021 07:05:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:05:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:05:14: #1 tags after filtering in treatment: 15191674 INFO @ Sat, 11 Dec 2021 07:05:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:05:14: #1 finished! INFO @ Sat, 11 Dec 2021 07:05:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:05:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:05:15: #2 number of paired peaks: 51 WARNING @ Sat, 11 Dec 2021 07:05:15: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:05:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:05:15: 9000000 INFO @ Sat, 11 Dec 2021 07:05:21: 10000000 INFO @ Sat, 11 Dec 2021 07:05:27: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:05:33: 12000000 INFO @ Sat, 11 Dec 2021 07:05:39: 13000000 INFO @ Sat, 11 Dec 2021 07:05:44: 14000000 INFO @ Sat, 11 Dec 2021 07:05:50: 15000000 INFO @ Sat, 11 Dec 2021 07:05:51: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:05:51: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:05:51: #1 total tags in treatment: 15191674 INFO @ Sat, 11 Dec 2021 07:05:51: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:05:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:05:51: #1 tags after filtering in treatment: 15191674 INFO @ Sat, 11 Dec 2021 07:05:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:05:51: #1 finished! INFO @ Sat, 11 Dec 2021 07:05:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:05:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:05:52: #2 number of paired peaks: 51 WARNING @ Sat, 11 Dec 2021 07:05:52: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:05:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689039/SRX8689039.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。