Job ID = 14170473 SRX = SRX8689038 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 18402995 spots for SRR12174237/SRR12174237.sra Written 18402995 spots for SRR12174237/SRR12174237.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170907 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:47 18402995 reads; of these: 18402995 (100.00%) were unpaired; of these: 466317 (2.53%) aligned 0 times 12769741 (69.39%) aligned exactly 1 time 5166937 (28.08%) aligned >1 times 97.47% overall alignment rate Time searching: 00:07:47 Overall time: 00:07:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1267833 / 17936678 = 0.0707 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:07:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:07:47: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:07:47: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:07:53: 1000000 INFO @ Sat, 11 Dec 2021 07:07:58: 2000000 INFO @ Sat, 11 Dec 2021 07:08:04: 3000000 INFO @ Sat, 11 Dec 2021 07:08:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:08:16: 5000000 INFO @ Sat, 11 Dec 2021 07:08:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:08:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:08:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:08:22: 6000000 INFO @ Sat, 11 Dec 2021 07:08:27: 1000000 INFO @ Sat, 11 Dec 2021 07:08:28: 7000000 INFO @ Sat, 11 Dec 2021 07:08:34: 8000000 INFO @ Sat, 11 Dec 2021 07:08:34: 2000000 INFO @ Sat, 11 Dec 2021 07:08:40: 9000000 INFO @ Sat, 11 Dec 2021 07:08:41: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:08:46: 10000000 INFO @ Sat, 11 Dec 2021 07:08:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:08:47: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:08:47: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:08:49: 4000000 INFO @ Sat, 11 Dec 2021 07:08:52: 11000000 INFO @ Sat, 11 Dec 2021 07:08:54: 1000000 INFO @ Sat, 11 Dec 2021 07:08:57: 5000000 INFO @ Sat, 11 Dec 2021 07:08:59: 12000000 INFO @ Sat, 11 Dec 2021 07:09:00: 2000000 INFO @ Sat, 11 Dec 2021 07:09:04: 6000000 INFO @ Sat, 11 Dec 2021 07:09:05: 13000000 INFO @ Sat, 11 Dec 2021 07:09:07: 3000000 INFO @ Sat, 11 Dec 2021 07:09:10: 7000000 INFO @ Sat, 11 Dec 2021 07:09:11: 14000000 INFO @ Sat, 11 Dec 2021 07:09:13: 4000000 INFO @ Sat, 11 Dec 2021 07:09:17: 8000000 INFO @ Sat, 11 Dec 2021 07:09:18: 15000000 INFO @ Sat, 11 Dec 2021 07:09:20: 5000000 INFO @ Sat, 11 Dec 2021 07:09:24: 9000000 INFO @ Sat, 11 Dec 2021 07:09:24: 16000000 INFO @ Sat, 11 Dec 2021 07:09:26: 6000000 INFO @ Sat, 11 Dec 2021 07:09:29: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:09:29: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:09:29: #1 total tags in treatment: 16668845 INFO @ Sat, 11 Dec 2021 07:09:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:09:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:09:29: #1 tags after filtering in treatment: 16668845 INFO @ Sat, 11 Dec 2021 07:09:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:09:29: #1 finished! INFO @ Sat, 11 Dec 2021 07:09:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:09:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:09:30: #2 number of paired peaks: 34 WARNING @ Sat, 11 Dec 2021 07:09:30: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:09:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:09:30: 10000000 INFO @ Sat, 11 Dec 2021 07:09:32: 7000000 INFO @ Sat, 11 Dec 2021 07:09:37: 11000000 INFO @ Sat, 11 Dec 2021 07:09:38: 8000000 INFO @ Sat, 11 Dec 2021 07:09:43: 12000000 INFO @ Sat, 11 Dec 2021 07:09:43: 9000000 INFO @ Sat, 11 Dec 2021 07:09:49: 13000000 INFO @ Sat, 11 Dec 2021 07:09:49: 10000000 INFO @ Sat, 11 Dec 2021 07:09:55: 14000000 INFO @ Sat, 11 Dec 2021 07:09:55: 11000000 INFO @ Sat, 11 Dec 2021 07:10:01: 15000000 INFO @ Sat, 11 Dec 2021 07:10:01: 12000000 INFO @ Sat, 11 Dec 2021 07:10:08: 13000000 INFO @ Sat, 11 Dec 2021 07:10:08: 16000000 INFO @ Sat, 11 Dec 2021 07:10:13: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:10:13: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:10:13: #1 total tags in treatment: 16668845 INFO @ Sat, 11 Dec 2021 07:10:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:10:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:10:13: #1 tags after filtering in treatment: 16668845 INFO @ Sat, 11 Dec 2021 07:10:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:10:13: #1 finished! INFO @ Sat, 11 Dec 2021 07:10:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:10:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:10:14: 14000000 INFO @ Sat, 11 Dec 2021 07:10:14: #2 number of paired peaks: 34 WARNING @ Sat, 11 Dec 2021 07:10:14: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:10:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:10:20: 15000000 INFO @ Sat, 11 Dec 2021 07:10:26: 16000000 INFO @ Sat, 11 Dec 2021 07:10:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 07:10:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 07:10:30: #1 total tags in treatment: 16668845 INFO @ Sat, 11 Dec 2021 07:10:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:10:30: #1 tags after filtering in treatment: 16668845 INFO @ Sat, 11 Dec 2021 07:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:10:30: #1 finished! INFO @ Sat, 11 Dec 2021 07:10:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:10:31: #2 number of paired peaks: 34 WARNING @ Sat, 11 Dec 2021 07:10:31: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:10:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689038/SRX8689038.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。