Job ID = 14170380 SRX = SRX8689004 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22897659 spots for SRR12174235/SRR12174235.sra Written 22897659 spots for SRR12174235/SRR12174235.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170787 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:10 22897659 reads; of these: 22897659 (100.00%) were unpaired; of these: 697946 (3.05%) aligned 0 times 14956359 (65.32%) aligned exactly 1 time 7243354 (31.63%) aligned >1 times 96.95% overall alignment rate Time searching: 00:09:11 Overall time: 00:09:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2206525 / 22199713 = 0.0994 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:43:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:43:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:43:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:44:03: 1000000 INFO @ Sat, 11 Dec 2021 06:44:09: 2000000 INFO @ Sat, 11 Dec 2021 06:44:15: 3000000 INFO @ Sat, 11 Dec 2021 06:44:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:44:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:44:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:44:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:44:28: 5000000 INFO @ Sat, 11 Dec 2021 06:44:34: 1000000 INFO @ Sat, 11 Dec 2021 06:44:35: 6000000 INFO @ Sat, 11 Dec 2021 06:44:41: 2000000 INFO @ Sat, 11 Dec 2021 06:44:42: 7000000 INFO @ Sat, 11 Dec 2021 06:44:48: 3000000 INFO @ Sat, 11 Dec 2021 06:44:49: 8000000 INFO @ Sat, 11 Dec 2021 06:44:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:44:56: 9000000 INFO @ Sat, 11 Dec 2021 06:44:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:44:57: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:44:57: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:45:01: 5000000 INFO @ Sat, 11 Dec 2021 06:45:03: 10000000 INFO @ Sat, 11 Dec 2021 06:45:04: 1000000 INFO @ Sat, 11 Dec 2021 06:45:08: 6000000 INFO @ Sat, 11 Dec 2021 06:45:10: 11000000 INFO @ Sat, 11 Dec 2021 06:45:11: 2000000 INFO @ Sat, 11 Dec 2021 06:45:16: 7000000 INFO @ Sat, 11 Dec 2021 06:45:17: 12000000 INFO @ Sat, 11 Dec 2021 06:45:18: 3000000 INFO @ Sat, 11 Dec 2021 06:45:23: 8000000 INFO @ Sat, 11 Dec 2021 06:45:24: 13000000 INFO @ Sat, 11 Dec 2021 06:45:25: 4000000 INFO @ Sat, 11 Dec 2021 06:45:30: 9000000 INFO @ Sat, 11 Dec 2021 06:45:31: 14000000 INFO @ Sat, 11 Dec 2021 06:45:32: 5000000 INFO @ Sat, 11 Dec 2021 06:45:37: 10000000 INFO @ Sat, 11 Dec 2021 06:45:38: 15000000 INFO @ Sat, 11 Dec 2021 06:45:39: 6000000 INFO @ Sat, 11 Dec 2021 06:45:44: 11000000 INFO @ Sat, 11 Dec 2021 06:45:45: 16000000 INFO @ Sat, 11 Dec 2021 06:45:46: 7000000 INFO @ Sat, 11 Dec 2021 06:45:51: 12000000 INFO @ Sat, 11 Dec 2021 06:45:52: 17000000 INFO @ Sat, 11 Dec 2021 06:45:53: 8000000 INFO @ Sat, 11 Dec 2021 06:45:58: 13000000 INFO @ Sat, 11 Dec 2021 06:45:59: 18000000 INFO @ Sat, 11 Dec 2021 06:46:00: 9000000 INFO @ Sat, 11 Dec 2021 06:46:05: 14000000 INFO @ Sat, 11 Dec 2021 06:46:06: 19000000 INFO @ Sat, 11 Dec 2021 06:46:07: 10000000 INFO @ Sat, 11 Dec 2021 06:46:12: 15000000 INFO @ Sat, 11 Dec 2021 06:46:13: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:46:13: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:46:13: #1 total tags in treatment: 19993188 INFO @ Sat, 11 Dec 2021 06:46:13: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:46:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:46:14: #1 tags after filtering in treatment: 19993188 INFO @ Sat, 11 Dec 2021 06:46:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:46:14: #1 finished! INFO @ Sat, 11 Dec 2021 06:46:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:46:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:46:14: 11000000 INFO @ Sat, 11 Dec 2021 06:46:15: #2 number of paired peaks: 70 WARNING @ Sat, 11 Dec 2021 06:46:15: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:46:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:46:19: 16000000 INFO @ Sat, 11 Dec 2021 06:46:21: 12000000 INFO @ Sat, 11 Dec 2021 06:46:27: 17000000 INFO @ Sat, 11 Dec 2021 06:46:28: 13000000 INFO @ Sat, 11 Dec 2021 06:46:34: 18000000 INFO @ Sat, 11 Dec 2021 06:46:35: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:46:41: 19000000 INFO @ Sat, 11 Dec 2021 06:46:42: 15000000 INFO @ Sat, 11 Dec 2021 06:46:48: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:46:48: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:46:48: #1 total tags in treatment: 19993188 INFO @ Sat, 11 Dec 2021 06:46:48: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:46:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:46:48: #1 tags after filtering in treatment: 19993188 INFO @ Sat, 11 Dec 2021 06:46:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:46:48: #1 finished! INFO @ Sat, 11 Dec 2021 06:46:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:46:49: 16000000 INFO @ Sat, 11 Dec 2021 06:46:50: #2 number of paired peaks: 70 WARNING @ Sat, 11 Dec 2021 06:46:50: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:46:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:46:56: 17000000 INFO @ Sat, 11 Dec 2021 06:47:02: 18000000 INFO @ Sat, 11 Dec 2021 06:47:09: 19000000 INFO @ Sat, 11 Dec 2021 06:47:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:47:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:47:15: #1 total tags in treatment: 19993188 INFO @ Sat, 11 Dec 2021 06:47:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:47:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:47:16: #1 tags after filtering in treatment: 19993188 INFO @ Sat, 11 Dec 2021 06:47:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:47:16: #1 finished! INFO @ Sat, 11 Dec 2021 06:47:16: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:47:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:47:17: #2 number of paired peaks: 70 WARNING @ Sat, 11 Dec 2021 06:47:17: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:47:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689004/SRX8689004.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。