Job ID = 14170376 SRX = SRX8689000 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21040672 spots for SRR12174231/SRR12174231.sra Written 21040672 spots for SRR12174231/SRR12174231.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170768 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:27 21040672 reads; of these: 21040672 (100.00%) were unpaired; of these: 568166 (2.70%) aligned 0 times 15260753 (72.53%) aligned exactly 1 time 5211753 (24.77%) aligned >1 times 97.30% overall alignment rate Time searching: 00:06:27 Overall time: 00:06:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1427854 / 20472506 = 0.0697 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:38:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:38:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:38:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:38:07: 1000000 INFO @ Sat, 11 Dec 2021 06:38:13: 2000000 INFO @ Sat, 11 Dec 2021 06:38:18: 3000000 INFO @ Sat, 11 Dec 2021 06:38:23: 4000000 INFO @ Sat, 11 Dec 2021 06:38:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:38:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:38:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:38:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:38:34: 6000000 INFO @ Sat, 11 Dec 2021 06:38:38: 1000000 INFO @ Sat, 11 Dec 2021 06:38:41: 7000000 INFO @ Sat, 11 Dec 2021 06:38:44: 2000000 INFO @ Sat, 11 Dec 2021 06:38:47: 8000000 INFO @ Sat, 11 Dec 2021 06:38:50: 3000000 INFO @ Sat, 11 Dec 2021 06:38:53: 9000000 INFO @ Sat, 11 Dec 2021 06:38:56: 4000000 INFO @ Sat, 11 Dec 2021 06:38:59: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:39:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:39:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:39:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:39:02: 5000000 INFO @ Sat, 11 Dec 2021 06:39:05: 11000000 INFO @ Sat, 11 Dec 2021 06:39:08: 1000000 INFO @ Sat, 11 Dec 2021 06:39:08: 6000000 INFO @ Sat, 11 Dec 2021 06:39:11: 12000000 INFO @ Sat, 11 Dec 2021 06:39:14: 2000000 INFO @ Sat, 11 Dec 2021 06:39:14: 7000000 INFO @ Sat, 11 Dec 2021 06:39:17: 13000000 INFO @ Sat, 11 Dec 2021 06:39:20: 3000000 INFO @ Sat, 11 Dec 2021 06:39:20: 8000000 INFO @ Sat, 11 Dec 2021 06:39:24: 14000000 INFO @ Sat, 11 Dec 2021 06:39:27: 4000000 INFO @ Sat, 11 Dec 2021 06:39:27: 9000000 INFO @ Sat, 11 Dec 2021 06:39:30: 15000000 INFO @ Sat, 11 Dec 2021 06:39:33: 10000000 INFO @ Sat, 11 Dec 2021 06:39:33: 5000000 INFO @ Sat, 11 Dec 2021 06:39:36: 16000000 INFO @ Sat, 11 Dec 2021 06:39:39: 11000000 INFO @ Sat, 11 Dec 2021 06:39:39: 6000000 INFO @ Sat, 11 Dec 2021 06:39:42: 17000000 INFO @ Sat, 11 Dec 2021 06:39:45: 12000000 INFO @ Sat, 11 Dec 2021 06:39:45: 7000000 INFO @ Sat, 11 Dec 2021 06:39:49: 18000000 INFO @ Sat, 11 Dec 2021 06:39:51: 13000000 INFO @ Sat, 11 Dec 2021 06:39:51: 8000000 INFO @ Sat, 11 Dec 2021 06:39:55: 19000000 INFO @ Sat, 11 Dec 2021 06:39:55: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:39:55: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:39:55: #1 total tags in treatment: 19044652 INFO @ Sat, 11 Dec 2021 06:39:55: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:39:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:39:55: #1 tags after filtering in treatment: 19044652 INFO @ Sat, 11 Dec 2021 06:39:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:39:55: #1 finished! INFO @ Sat, 11 Dec 2021 06:39:55: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:39:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:39:57: #2 number of paired peaks: 28 WARNING @ Sat, 11 Dec 2021 06:39:57: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:39:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:39:58: 14000000 INFO @ Sat, 11 Dec 2021 06:39:58: 9000000 INFO @ Sat, 11 Dec 2021 06:40:04: 15000000 INFO @ Sat, 11 Dec 2021 06:40:04: 10000000 INFO @ Sat, 11 Dec 2021 06:40:10: 16000000 INFO @ Sat, 11 Dec 2021 06:40:10: 11000000 INFO @ Sat, 11 Dec 2021 06:40:16: 12000000 INFO @ Sat, 11 Dec 2021 06:40:16: 17000000 INFO @ Sat, 11 Dec 2021 06:40:22: 18000000 INFO @ Sat, 11 Dec 2021 06:40:22: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:40:28: 19000000 INFO @ Sat, 11 Dec 2021 06:40:29: 14000000 INFO @ Sat, 11 Dec 2021 06:40:29: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:40:29: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:40:29: #1 total tags in treatment: 19044652 INFO @ Sat, 11 Dec 2021 06:40:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:40:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:40:29: #1 tags after filtering in treatment: 19044652 INFO @ Sat, 11 Dec 2021 06:40:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:40:29: #1 finished! INFO @ Sat, 11 Dec 2021 06:40:29: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:40:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:40:30: #2 number of paired peaks: 28 WARNING @ Sat, 11 Dec 2021 06:40:30: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:40:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:40:34: 15000000 INFO @ Sat, 11 Dec 2021 06:40:40: 16000000 INFO @ Sat, 11 Dec 2021 06:40:46: 17000000 INFO @ Sat, 11 Dec 2021 06:40:52: 18000000 INFO @ Sat, 11 Dec 2021 06:40:58: 19000000 INFO @ Sat, 11 Dec 2021 06:40:58: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 06:40:58: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 06:40:58: #1 total tags in treatment: 19044652 INFO @ Sat, 11 Dec 2021 06:40:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:40:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:40:58: #1 tags after filtering in treatment: 19044652 INFO @ Sat, 11 Dec 2021 06:40:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 06:40:58: #1 finished! INFO @ Sat, 11 Dec 2021 06:40:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:40:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:41:00: #2 number of paired peaks: 28 WARNING @ Sat, 11 Dec 2021 06:41:00: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 06:41:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8689000/SRX8689000.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。