Job ID = 14168250 SRX = SRX8688999 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20428931 spots for SRR12174230/SRR12174230.sra Written 20428931 spots for SRR12174230/SRR12174230.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169125 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:24 20428931 reads; of these: 20428931 (100.00%) were unpaired; of these: 587385 (2.88%) aligned 0 times 14666355 (71.79%) aligned exactly 1 time 5175191 (25.33%) aligned >1 times 97.12% overall alignment rate Time searching: 00:08:24 Overall time: 00:08:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1393383 / 19841546 = 0.0702 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:50:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:50:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:50:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:50:06: 1000000 INFO @ Fri, 10 Dec 2021 16:50:12: 2000000 INFO @ Fri, 10 Dec 2021 16:50:17: 3000000 INFO @ Fri, 10 Dec 2021 16:50:23: 4000000 INFO @ Fri, 10 Dec 2021 16:50:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:50:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:50:31: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:50:31: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:50:34: 6000000 INFO @ Fri, 10 Dec 2021 16:50:37: 1000000 INFO @ Fri, 10 Dec 2021 16:50:40: 7000000 INFO @ Fri, 10 Dec 2021 16:50:43: 2000000 INFO @ Fri, 10 Dec 2021 16:50:46: 8000000 INFO @ Fri, 10 Dec 2021 16:50:49: 3000000 INFO @ Fri, 10 Dec 2021 16:50:52: 9000000 INFO @ Fri, 10 Dec 2021 16:50:55: 4000000 INFO @ Fri, 10 Dec 2021 16:50:58: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:51:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:51:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:51:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:51:01: 5000000 INFO @ Fri, 10 Dec 2021 16:51:04: 11000000 INFO @ Fri, 10 Dec 2021 16:51:07: 1000000 INFO @ Fri, 10 Dec 2021 16:51:08: 6000000 INFO @ Fri, 10 Dec 2021 16:51:11: 12000000 INFO @ Fri, 10 Dec 2021 16:51:13: 2000000 INFO @ Fri, 10 Dec 2021 16:51:14: 7000000 INFO @ Fri, 10 Dec 2021 16:51:17: 13000000 INFO @ Fri, 10 Dec 2021 16:51:20: 3000000 INFO @ Fri, 10 Dec 2021 16:51:20: 8000000 INFO @ Fri, 10 Dec 2021 16:51:23: 14000000 INFO @ Fri, 10 Dec 2021 16:51:26: 4000000 INFO @ Fri, 10 Dec 2021 16:51:27: 9000000 INFO @ Fri, 10 Dec 2021 16:51:29: 15000000 INFO @ Fri, 10 Dec 2021 16:51:32: 5000000 INFO @ Fri, 10 Dec 2021 16:51:33: 10000000 INFO @ Fri, 10 Dec 2021 16:51:36: 16000000 INFO @ Fri, 10 Dec 2021 16:51:38: 6000000 INFO @ Fri, 10 Dec 2021 16:51:39: 11000000 INFO @ Fri, 10 Dec 2021 16:51:42: 17000000 INFO @ Fri, 10 Dec 2021 16:51:45: 7000000 INFO @ Fri, 10 Dec 2021 16:51:46: 12000000 INFO @ Fri, 10 Dec 2021 16:51:48: 18000000 INFO @ Fri, 10 Dec 2021 16:51:51: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:51:51: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:51:51: #1 total tags in treatment: 18448163 INFO @ Fri, 10 Dec 2021 16:51:51: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:51:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:51:51: 8000000 INFO @ Fri, 10 Dec 2021 16:51:51: #1 tags after filtering in treatment: 18448163 INFO @ Fri, 10 Dec 2021 16:51:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:51:51: #1 finished! INFO @ Fri, 10 Dec 2021 16:51:51: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:51:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:51:52: 13000000 INFO @ Fri, 10 Dec 2021 16:51:52: #2 number of paired peaks: 28 WARNING @ Fri, 10 Dec 2021 16:51:52: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:51:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:51:57: 9000000 INFO @ Fri, 10 Dec 2021 16:51:58: 14000000 INFO @ Fri, 10 Dec 2021 16:52:03: 10000000 INFO @ Fri, 10 Dec 2021 16:52:04: 15000000 INFO @ Fri, 10 Dec 2021 16:52:10: 11000000 INFO @ Fri, 10 Dec 2021 16:52:11: 16000000 INFO @ Fri, 10 Dec 2021 16:52:16: 12000000 INFO @ Fri, 10 Dec 2021 16:52:17: 17000000 INFO @ Fri, 10 Dec 2021 16:52:22: 13000000 INFO @ Fri, 10 Dec 2021 16:52:23: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:52:26: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:52:26: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:52:26: #1 total tags in treatment: 18448163 INFO @ Fri, 10 Dec 2021 16:52:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:52:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:52:26: #1 tags after filtering in treatment: 18448163 INFO @ Fri, 10 Dec 2021 16:52:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:52:26: #1 finished! INFO @ Fri, 10 Dec 2021 16:52:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:52:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:52:28: #2 number of paired peaks: 28 WARNING @ Fri, 10 Dec 2021 16:52:28: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:52:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:52:28: 14000000 INFO @ Fri, 10 Dec 2021 16:52:34: 15000000 INFO @ Fri, 10 Dec 2021 16:52:39: 16000000 INFO @ Fri, 10 Dec 2021 16:52:45: 17000000 INFO @ Fri, 10 Dec 2021 16:52:50: 18000000 INFO @ Fri, 10 Dec 2021 16:52:53: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:52:53: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:52:53: #1 total tags in treatment: 18448163 INFO @ Fri, 10 Dec 2021 16:52:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:52:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:52:53: #1 tags after filtering in treatment: 18448163 INFO @ Fri, 10 Dec 2021 16:52:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:52:53: #1 finished! INFO @ Fri, 10 Dec 2021 16:52:53: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:52:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:52:54: #2 number of paired peaks: 28 WARNING @ Fri, 10 Dec 2021 16:52:54: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:52:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688999/SRX8688999.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。