Job ID = 14168249 SRX = SRX8688998 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21498167 spots for SRR12174229/SRR12174229.sra Written 21498167 spots for SRR12174229/SRR12174229.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169120 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:09 21498167 reads; of these: 21498167 (100.00%) were unpaired; of these: 662257 (3.08%) aligned 0 times 15306599 (71.20%) aligned exactly 1 time 5529311 (25.72%) aligned >1 times 96.92% overall alignment rate Time searching: 00:07:09 Overall time: 00:07:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1449329 / 20835910 = 0.0696 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:47:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:47:40: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:47:40: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:47:47: 1000000 INFO @ Fri, 10 Dec 2021 16:47:54: 2000000 INFO @ Fri, 10 Dec 2021 16:48:02: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:48:09: 4000000 INFO @ Fri, 10 Dec 2021 16:48:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:48:10: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:48:10: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:48:17: 5000000 INFO @ Fri, 10 Dec 2021 16:48:17: 1000000 INFO @ Fri, 10 Dec 2021 16:48:25: 2000000 INFO @ Fri, 10 Dec 2021 16:48:25: 6000000 INFO @ Fri, 10 Dec 2021 16:48:33: 3000000 INFO @ Fri, 10 Dec 2021 16:48:33: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:48:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:48:40: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:48:40: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:48:40: 4000000 INFO @ Fri, 10 Dec 2021 16:48:41: 8000000 INFO @ Fri, 10 Dec 2021 16:48:47: 1000000 INFO @ Fri, 10 Dec 2021 16:48:48: 5000000 INFO @ Fri, 10 Dec 2021 16:48:50: 9000000 INFO @ Fri, 10 Dec 2021 16:48:55: 2000000 INFO @ Fri, 10 Dec 2021 16:48:56: 6000000 INFO @ Fri, 10 Dec 2021 16:48:58: 10000000 INFO @ Fri, 10 Dec 2021 16:49:03: 3000000 INFO @ Fri, 10 Dec 2021 16:49:03: 7000000 INFO @ Fri, 10 Dec 2021 16:49:06: 11000000 INFO @ Fri, 10 Dec 2021 16:49:10: 4000000 INFO @ Fri, 10 Dec 2021 16:49:11: 8000000 INFO @ Fri, 10 Dec 2021 16:49:14: 12000000 INFO @ Fri, 10 Dec 2021 16:49:18: 5000000 INFO @ Fri, 10 Dec 2021 16:49:19: 9000000 INFO @ Fri, 10 Dec 2021 16:49:23: 13000000 INFO @ Fri, 10 Dec 2021 16:49:25: 6000000 INFO @ Fri, 10 Dec 2021 16:49:26: 10000000 INFO @ Fri, 10 Dec 2021 16:49:31: 14000000 INFO @ Fri, 10 Dec 2021 16:49:33: 7000000 INFO @ Fri, 10 Dec 2021 16:49:34: 11000000 INFO @ Fri, 10 Dec 2021 16:49:40: 15000000 INFO @ Fri, 10 Dec 2021 16:49:41: 8000000 INFO @ Fri, 10 Dec 2021 16:49:41: 12000000 INFO @ Fri, 10 Dec 2021 16:49:48: 9000000 INFO @ Fri, 10 Dec 2021 16:49:49: 16000000 INFO @ Fri, 10 Dec 2021 16:49:49: 13000000 INFO @ Fri, 10 Dec 2021 16:49:56: 10000000 INFO @ Fri, 10 Dec 2021 16:49:57: 14000000 INFO @ Fri, 10 Dec 2021 16:49:57: 17000000 INFO @ Fri, 10 Dec 2021 16:50:03: 11000000 INFO @ Fri, 10 Dec 2021 16:50:04: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:50:06: 18000000 INFO @ Fri, 10 Dec 2021 16:50:11: 12000000 INFO @ Fri, 10 Dec 2021 16:50:12: 16000000 INFO @ Fri, 10 Dec 2021 16:50:14: 19000000 INFO @ Fri, 10 Dec 2021 16:50:18: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:50:18: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:50:18: #1 total tags in treatment: 19386581 INFO @ Fri, 10 Dec 2021 16:50:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:50:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:50:18: #1 tags after filtering in treatment: 19386581 INFO @ Fri, 10 Dec 2021 16:50:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:50:18: #1 finished! INFO @ Fri, 10 Dec 2021 16:50:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:50:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:50:18: 13000000 INFO @ Fri, 10 Dec 2021 16:50:19: #2 number of paired peaks: 25 WARNING @ Fri, 10 Dec 2021 16:50:19: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:50:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:50:20: 17000000 INFO @ Fri, 10 Dec 2021 16:50:26: 14000000 INFO @ Fri, 10 Dec 2021 16:50:27: 18000000 INFO @ Fri, 10 Dec 2021 16:50:33: 15000000 INFO @ Fri, 10 Dec 2021 16:50:34: 19000000 INFO @ Fri, 10 Dec 2021 16:50:37: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:50:37: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:50:37: #1 total tags in treatment: 19386581 INFO @ Fri, 10 Dec 2021 16:50:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:50:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:50:37: #1 tags after filtering in treatment: 19386581 INFO @ Fri, 10 Dec 2021 16:50:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:50:37: #1 finished! INFO @ Fri, 10 Dec 2021 16:50:37: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:50:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:50:39: #2 number of paired peaks: 25 WARNING @ Fri, 10 Dec 2021 16:50:39: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:50:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:50:40: 16000000 INFO @ Fri, 10 Dec 2021 16:50:47: 17000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:50:54: 18000000 INFO @ Fri, 10 Dec 2021 16:51:01: 19000000 INFO @ Fri, 10 Dec 2021 16:51:03: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 16:51:03: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 16:51:03: #1 total tags in treatment: 19386581 INFO @ Fri, 10 Dec 2021 16:51:03: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:51:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:51:04: #1 tags after filtering in treatment: 19386581 INFO @ Fri, 10 Dec 2021 16:51:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 16:51:04: #1 finished! INFO @ Fri, 10 Dec 2021 16:51:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:51:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:51:05: #2 number of paired peaks: 25 WARNING @ Fri, 10 Dec 2021 16:51:05: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:51:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688998/SRX8688998.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling