Job ID = 14167497 SRX = SRX8688957 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23949992 spots for SRR12174228/SRR12174228.sra Written 23949992 spots for SRR12174228/SRR12174228.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168103 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:07 23949992 reads; of these: 23949992 (100.00%) were unpaired; of these: 834766 (3.49%) aligned 0 times 17216357 (71.88%) aligned exactly 1 time 5898869 (24.63%) aligned >1 times 96.51% overall alignment rate Time searching: 00:08:07 Overall time: 00:08:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1747820 / 23115226 = 0.0756 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:43:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:43:10: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:43:10: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:43:15: 1000000 INFO @ Fri, 10 Dec 2021 12:43:20: 2000000 INFO @ Fri, 10 Dec 2021 12:43:25: 3000000 INFO @ Fri, 10 Dec 2021 12:43:30: 4000000 INFO @ Fri, 10 Dec 2021 12:43:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:43:40: 6000000 INFO @ Fri, 10 Dec 2021 12:43:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:43:40: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:43:40: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:43:45: 7000000 INFO @ Fri, 10 Dec 2021 12:43:46: 1000000 INFO @ Fri, 10 Dec 2021 12:43:50: 8000000 INFO @ Fri, 10 Dec 2021 12:43:52: 2000000 INFO @ Fri, 10 Dec 2021 12:43:56: 9000000 INFO @ Fri, 10 Dec 2021 12:43:58: 3000000 INFO @ Fri, 10 Dec 2021 12:44:01: 10000000 INFO @ Fri, 10 Dec 2021 12:44:05: 4000000 INFO @ Fri, 10 Dec 2021 12:44:07: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:44:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:44:10: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:44:10: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:44:11: 5000000 INFO @ Fri, 10 Dec 2021 12:44:12: 12000000 INFO @ Fri, 10 Dec 2021 12:44:16: 1000000 INFO @ Fri, 10 Dec 2021 12:44:17: 6000000 INFO @ Fri, 10 Dec 2021 12:44:17: 13000000 INFO @ Fri, 10 Dec 2021 12:44:21: 2000000 INFO @ Fri, 10 Dec 2021 12:44:23: 14000000 INFO @ Fri, 10 Dec 2021 12:44:23: 7000000 INFO @ Fri, 10 Dec 2021 12:44:26: 3000000 INFO @ Fri, 10 Dec 2021 12:44:28: 15000000 INFO @ Fri, 10 Dec 2021 12:44:29: 8000000 INFO @ Fri, 10 Dec 2021 12:44:32: 4000000 INFO @ Fri, 10 Dec 2021 12:44:33: 16000000 INFO @ Fri, 10 Dec 2021 12:44:35: 9000000 INFO @ Fri, 10 Dec 2021 12:44:37: 5000000 INFO @ Fri, 10 Dec 2021 12:44:38: 17000000 INFO @ Fri, 10 Dec 2021 12:44:42: 10000000 INFO @ Fri, 10 Dec 2021 12:44:43: 6000000 INFO @ Fri, 10 Dec 2021 12:44:44: 18000000 INFO @ Fri, 10 Dec 2021 12:44:48: 11000000 INFO @ Fri, 10 Dec 2021 12:44:48: 7000000 INFO @ Fri, 10 Dec 2021 12:44:49: 19000000 INFO @ Fri, 10 Dec 2021 12:44:54: 8000000 INFO @ Fri, 10 Dec 2021 12:44:54: 12000000 INFO @ Fri, 10 Dec 2021 12:44:54: 20000000 INFO @ Fri, 10 Dec 2021 12:44:59: 9000000 INFO @ Fri, 10 Dec 2021 12:45:00: 13000000 INFO @ Fri, 10 Dec 2021 12:45:00: 21000000 INFO @ Fri, 10 Dec 2021 12:45:02: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:45:02: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:45:02: #1 total tags in treatment: 21367406 INFO @ Fri, 10 Dec 2021 12:45:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:45:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:45:02: #1 tags after filtering in treatment: 21367406 INFO @ Fri, 10 Dec 2021 12:45:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:45:02: #1 finished! INFO @ Fri, 10 Dec 2021 12:45:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:45:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:45:03: #2 number of paired peaks: 19 WARNING @ Fri, 10 Dec 2021 12:45:03: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:45:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:45:04: 10000000 INFO @ Fri, 10 Dec 2021 12:45:06: 14000000 INFO @ Fri, 10 Dec 2021 12:45:10: 11000000 INFO @ Fri, 10 Dec 2021 12:45:11: 15000000 INFO @ Fri, 10 Dec 2021 12:45:15: 12000000 INFO @ Fri, 10 Dec 2021 12:45:17: 16000000 INFO @ Fri, 10 Dec 2021 12:45:20: 13000000 INFO @ Fri, 10 Dec 2021 12:45:23: 17000000 INFO @ Fri, 10 Dec 2021 12:45:25: 14000000 INFO @ Fri, 10 Dec 2021 12:45:29: 18000000 INFO @ Fri, 10 Dec 2021 12:45:31: 15000000 INFO @ Fri, 10 Dec 2021 12:45:35: 19000000 INFO @ Fri, 10 Dec 2021 12:45:36: 16000000 INFO @ Fri, 10 Dec 2021 12:45:41: 20000000 INFO @ Fri, 10 Dec 2021 12:45:41: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:45:47: 18000000 INFO @ Fri, 10 Dec 2021 12:45:47: 21000000 INFO @ Fri, 10 Dec 2021 12:45:49: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:45:49: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:45:49: #1 total tags in treatment: 21367406 INFO @ Fri, 10 Dec 2021 12:45:49: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:45:49: #1 tags after filtering in treatment: 21367406 INFO @ Fri, 10 Dec 2021 12:45:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:45:49: #1 finished! INFO @ Fri, 10 Dec 2021 12:45:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:45:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:45:51: #2 number of paired peaks: 19 WARNING @ Fri, 10 Dec 2021 12:45:51: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:45:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:45:52: 19000000 INFO @ Fri, 10 Dec 2021 12:45:57: 20000000 INFO @ Fri, 10 Dec 2021 12:46:02: 21000000 INFO @ Fri, 10 Dec 2021 12:46:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:46:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:46:04: #1 total tags in treatment: 21367406 INFO @ Fri, 10 Dec 2021 12:46:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:46:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:46:04: #1 tags after filtering in treatment: 21367406 INFO @ Fri, 10 Dec 2021 12:46:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:46:04: #1 finished! INFO @ Fri, 10 Dec 2021 12:46:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:46:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:46:06: #2 number of paired peaks: 19 WARNING @ Fri, 10 Dec 2021 12:46:06: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:46:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688957/SRX8688957.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。