Job ID = 14167476 SRX = SRX8688950 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6777993 spots for SRR12174221/SRR12174221.sra Written 6777993 spots for SRR12174221/SRR12174221.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168064 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:47 6777993 reads; of these: 6777993 (100.00%) were unpaired; of these: 924803 (13.64%) aligned 0 times 4700720 (69.35%) aligned exactly 1 time 1152470 (17.00%) aligned >1 times 86.36% overall alignment rate Time searching: 00:01:47 Overall time: 00:01:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 271422 / 5853190 = 0.0464 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:35: 1000000 INFO @ Fri, 10 Dec 2021 12:27:40: 2000000 INFO @ Fri, 10 Dec 2021 12:27:45: 3000000 INFO @ Fri, 10 Dec 2021 12:27:50: 4000000 INFO @ Fri, 10 Dec 2021 12:27:54: 5000000 INFO @ Fri, 10 Dec 2021 12:27:57: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:27:57: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:27:57: #1 total tags in treatment: 5581768 INFO @ Fri, 10 Dec 2021 12:27:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:27:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:27:57: #1 tags after filtering in treatment: 5581768 INFO @ Fri, 10 Dec 2021 12:27:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:27:57: #1 finished! INFO @ Fri, 10 Dec 2021 12:27:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:27:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:27:58: #2 number of paired peaks: 30 WARNING @ Fri, 10 Dec 2021 12:27:58: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:27:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:28:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:05: 1000000 INFO @ Fri, 10 Dec 2021 12:28:10: 2000000 INFO @ Fri, 10 Dec 2021 12:28:15: 3000000 INFO @ Fri, 10 Dec 2021 12:28:20: 4000000 INFO @ Fri, 10 Dec 2021 12:28:25: 5000000 INFO @ Fri, 10 Dec 2021 12:28:27: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:27: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:27: #1 total tags in treatment: 5581768 INFO @ Fri, 10 Dec 2021 12:28:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:28: #1 tags after filtering in treatment: 5581768 INFO @ Fri, 10 Dec 2021 12:28:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:28: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:28: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... INFO @ Fri, 10 Dec 2021 12:28:28: #2 number of paired peaks: 30 WARNING @ Fri, 10 Dec 2021 12:28:28: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:28: Process for pairing-model is terminated! WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:28:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:28:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:28:36: 1000000 INFO @ Fri, 10 Dec 2021 12:28:41: 2000000 INFO @ Fri, 10 Dec 2021 12:28:47: 3000000 INFO @ Fri, 10 Dec 2021 12:28:52: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:28:58: 5000000 INFO @ Fri, 10 Dec 2021 12:29:01: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:29:01: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:29:01: #1 total tags in treatment: 5581768 INFO @ Fri, 10 Dec 2021 12:29:01: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:29:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:29:02: #1 tags after filtering in treatment: 5581768 INFO @ Fri, 10 Dec 2021 12:29:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:29:02: #1 finished! INFO @ Fri, 10 Dec 2021 12:29:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:29:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:29:02: #2 number of paired peaks: 30 WARNING @ Fri, 10 Dec 2021 12:29:02: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:29:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688950/SRX8688950.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。