Job ID = 14167442 SRX = SRX8688949 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 20998262 spots for SRR12174180/SRR12174180.sra Written 20998262 spots for SRR12174180/SRR12174180.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168071 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:10 20998262 reads; of these: 20998262 (100.00%) were unpaired; of these: 798413 (3.80%) aligned 0 times 16143565 (76.88%) aligned exactly 1 time 4056284 (19.32%) aligned >1 times 96.20% overall alignment rate Time searching: 00:06:10 Overall time: 00:06:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1742978 / 20199849 = 0.0863 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:31:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:31:48: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:31:48: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:31:54: 1000000 INFO @ Fri, 10 Dec 2021 12:31:59: 2000000 INFO @ Fri, 10 Dec 2021 12:32:04: 3000000 INFO @ Fri, 10 Dec 2021 12:32:09: 4000000 INFO @ Fri, 10 Dec 2021 12:32:14: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:32:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:32:18: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:32:18: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:32:19: 6000000 INFO @ Fri, 10 Dec 2021 12:32:24: 1000000 INFO @ Fri, 10 Dec 2021 12:32:25: 7000000 INFO @ Fri, 10 Dec 2021 12:32:30: 2000000 INFO @ Fri, 10 Dec 2021 12:32:30: 8000000 INFO @ Fri, 10 Dec 2021 12:32:35: 3000000 INFO @ Fri, 10 Dec 2021 12:32:36: 9000000 INFO @ Fri, 10 Dec 2021 12:32:41: 4000000 INFO @ Fri, 10 Dec 2021 12:32:41: 10000000 INFO @ Fri, 10 Dec 2021 12:32:46: 5000000 INFO @ Fri, 10 Dec 2021 12:32:46: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:32:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:32:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:32:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:32:52: 6000000 INFO @ Fri, 10 Dec 2021 12:32:52: 12000000 INFO @ Fri, 10 Dec 2021 12:32:54: 1000000 INFO @ Fri, 10 Dec 2021 12:32:57: 7000000 INFO @ Fri, 10 Dec 2021 12:32:58: 13000000 INFO @ Fri, 10 Dec 2021 12:33:00: 2000000 INFO @ Fri, 10 Dec 2021 12:33:03: 8000000 INFO @ Fri, 10 Dec 2021 12:33:05: 14000000 INFO @ Fri, 10 Dec 2021 12:33:09: 9000000 INFO @ Fri, 10 Dec 2021 12:33:09: 3000000 INFO @ Fri, 10 Dec 2021 12:33:12: 15000000 INFO @ Fri, 10 Dec 2021 12:33:17: 10000000 INFO @ Fri, 10 Dec 2021 12:33:18: 4000000 INFO @ Fri, 10 Dec 2021 12:33:18: 16000000 INFO @ Fri, 10 Dec 2021 12:33:24: 11000000 INFO @ Fri, 10 Dec 2021 12:33:25: 5000000 INFO @ Fri, 10 Dec 2021 12:33:26: 17000000 INFO @ Fri, 10 Dec 2021 12:33:30: 12000000 INFO @ Fri, 10 Dec 2021 12:33:32: 18000000 INFO @ Fri, 10 Dec 2021 12:33:33: 6000000 INFO @ Fri, 10 Dec 2021 12:33:35: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:33:35: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:33:35: #1 total tags in treatment: 18456871 INFO @ Fri, 10 Dec 2021 12:33:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:33:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:33:36: #1 tags after filtering in treatment: 18456871 INFO @ Fri, 10 Dec 2021 12:33:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:33:36: #1 finished! INFO @ Fri, 10 Dec 2021 12:33:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:33:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:33:38: #2 number of paired peaks: 18 WARNING @ Fri, 10 Dec 2021 12:33:38: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:33:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 10 Dec 2021 12:33:38: 13000000 pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:33:40: 7000000 INFO @ Fri, 10 Dec 2021 12:33:46: 14000000 INFO @ Fri, 10 Dec 2021 12:33:48: 8000000 INFO @ Fri, 10 Dec 2021 12:33:53: 15000000 INFO @ Fri, 10 Dec 2021 12:33:56: 9000000 INFO @ Fri, 10 Dec 2021 12:34:01: 16000000 INFO @ Fri, 10 Dec 2021 12:34:04: 10000000 INFO @ Fri, 10 Dec 2021 12:34:09: 17000000 INFO @ Fri, 10 Dec 2021 12:34:12: 11000000 INFO @ Fri, 10 Dec 2021 12:34:16: 18000000 INFO @ Fri, 10 Dec 2021 12:34:20: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:34:20: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:34:20: #1 total tags in treatment: 18456871 INFO @ Fri, 10 Dec 2021 12:34:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:34:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:34:20: 12000000 INFO @ Fri, 10 Dec 2021 12:34:20: #1 tags after filtering in treatment: 18456871 INFO @ Fri, 10 Dec 2021 12:34:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:34:20: #1 finished! INFO @ Fri, 10 Dec 2021 12:34:20: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:34:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:34:22: #2 number of paired peaks: 18 WARNING @ Fri, 10 Dec 2021 12:34:22: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:34:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 45 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:34:28: 13000000 INFO @ Fri, 10 Dec 2021 12:34:36: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:34:43: 15000000 INFO @ Fri, 10 Dec 2021 12:34:49: 16000000 INFO @ Fri, 10 Dec 2021 12:34:55: 17000000 INFO @ Fri, 10 Dec 2021 12:35:01: 18000000 INFO @ Fri, 10 Dec 2021 12:35:04: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:35:04: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:35:04: #1 total tags in treatment: 18456871 INFO @ Fri, 10 Dec 2021 12:35:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:35:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:35:05: #1 tags after filtering in treatment: 18456871 INFO @ Fri, 10 Dec 2021 12:35:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:35:05: #1 finished! INFO @ Fri, 10 Dec 2021 12:35:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:35:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:35:06: #2 number of paired peaks: 18 WARNING @ Fri, 10 Dec 2021 12:35:06: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:35:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 9 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688949/SRX8688949.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。