Job ID = 14167433 SRX = SRX8688944 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21907683 spots for SRR12174175/SRR12174175.sra Written 21907683 spots for SRR12174175/SRR12174175.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168060 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 21907683 reads; of these: 21907683 (100.00%) were unpaired; of these: 5923469 (27.04%) aligned 0 times 12520797 (57.15%) aligned exactly 1 time 3463417 (15.81%) aligned >1 times 72.96% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1125690 / 15984214 = 0.0704 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:29:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:29:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:29:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:29:20: 1000000 INFO @ Fri, 10 Dec 2021 12:29:26: 2000000 INFO @ Fri, 10 Dec 2021 12:29:32: 3000000 INFO @ Fri, 10 Dec 2021 12:29:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:29:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:29:44: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:29:44: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:29:44: 5000000 INFO @ Fri, 10 Dec 2021 12:29:50: 1000000 INFO @ Fri, 10 Dec 2021 12:29:51: 6000000 INFO @ Fri, 10 Dec 2021 12:29:57: 2000000 INFO @ Fri, 10 Dec 2021 12:29:57: 7000000 INFO @ Fri, 10 Dec 2021 12:30:03: 8000000 INFO @ Fri, 10 Dec 2021 12:30:04: 3000000 INFO @ Fri, 10 Dec 2021 12:30:10: 9000000 INFO @ Fri, 10 Dec 2021 12:30:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:30:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:30:14: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:30:14: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:30:16: 10000000 INFO @ Fri, 10 Dec 2021 12:30:17: 5000000 INFO @ Fri, 10 Dec 2021 12:30:20: 1000000 INFO @ Fri, 10 Dec 2021 12:30:23: 11000000 INFO @ Fri, 10 Dec 2021 12:30:24: 6000000 INFO @ Fri, 10 Dec 2021 12:30:25: 2000000 INFO @ Fri, 10 Dec 2021 12:30:29: 12000000 INFO @ Fri, 10 Dec 2021 12:30:30: 7000000 INFO @ Fri, 10 Dec 2021 12:30:31: 3000000 INFO @ Fri, 10 Dec 2021 12:30:36: 13000000 INFO @ Fri, 10 Dec 2021 12:30:37: 8000000 INFO @ Fri, 10 Dec 2021 12:30:37: 4000000 INFO @ Fri, 10 Dec 2021 12:30:42: 14000000 INFO @ Fri, 10 Dec 2021 12:30:43: 5000000 INFO @ Fri, 10 Dec 2021 12:30:43: 9000000 INFO @ Fri, 10 Dec 2021 12:30:48: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:30:48: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:30:48: #1 total tags in treatment: 14858524 INFO @ Fri, 10 Dec 2021 12:30:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:30:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:30:48: #1 tags after filtering in treatment: 14858524 INFO @ Fri, 10 Dec 2021 12:30:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:30:48: #1 finished! INFO @ Fri, 10 Dec 2021 12:30:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:30:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:30:49: 6000000 INFO @ Fri, 10 Dec 2021 12:30:49: #2 number of paired peaks: 76 WARNING @ Fri, 10 Dec 2021 12:30:49: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:30:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:30:50: 10000000 INFO @ Fri, 10 Dec 2021 12:30:54: 7000000 INFO @ Fri, 10 Dec 2021 12:30:56: 11000000 INFO @ Fri, 10 Dec 2021 12:31:00: 8000000 INFO @ Fri, 10 Dec 2021 12:31:03: 12000000 INFO @ Fri, 10 Dec 2021 12:31:06: 9000000 INFO @ Fri, 10 Dec 2021 12:31:09: 13000000 INFO @ Fri, 10 Dec 2021 12:31:12: 10000000 INFO @ Fri, 10 Dec 2021 12:31:15: 14000000 INFO @ Fri, 10 Dec 2021 12:31:17: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:31:21: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:31:21: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:31:21: #1 total tags in treatment: 14858524 INFO @ Fri, 10 Dec 2021 12:31:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:31:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:31:21: #1 tags after filtering in treatment: 14858524 INFO @ Fri, 10 Dec 2021 12:31:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:31:21: #1 finished! INFO @ Fri, 10 Dec 2021 12:31:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:31:22: #2 number of paired peaks: 76 WARNING @ Fri, 10 Dec 2021 12:31:22: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:31:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:31:23: 12000000 INFO @ Fri, 10 Dec 2021 12:31:28: 13000000 INFO @ Fri, 10 Dec 2021 12:31:34: 14000000 INFO @ Fri, 10 Dec 2021 12:31:38: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:31:38: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:31:38: #1 total tags in treatment: 14858524 INFO @ Fri, 10 Dec 2021 12:31:38: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:31:38: #1 tags after filtering in treatment: 14858524 INFO @ Fri, 10 Dec 2021 12:31:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:31:38: #1 finished! INFO @ Fri, 10 Dec 2021 12:31:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:31:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:31:39: #2 number of paired peaks: 76 WARNING @ Fri, 10 Dec 2021 12:31:39: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:31:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688944/SRX8688944.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。