Job ID = 14167431 SRX = SRX8688942 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21750684 spots for SRR12174173/SRR12174173.sra Written 21750684 spots for SRR12174173/SRR12174173.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168050 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:07 21750684 reads; of these: 21750684 (100.00%) were unpaired; of these: 7859864 (36.14%) aligned 0 times 10892906 (50.08%) aligned exactly 1 time 2997914 (13.78%) aligned >1 times 63.86% overall alignment rate Time searching: 00:05:07 Overall time: 00:05:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 862610 / 13890820 = 0.0621 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:26:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:26:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:26:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:26:41: 1000000 INFO @ Fri, 10 Dec 2021 12:26:47: 2000000 INFO @ Fri, 10 Dec 2021 12:26:54: 3000000 INFO @ Fri, 10 Dec 2021 12:27:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:07: 5000000 INFO @ Fri, 10 Dec 2021 12:27:14: 1000000 INFO @ Fri, 10 Dec 2021 12:27:15: 6000000 INFO @ Fri, 10 Dec 2021 12:27:21: 2000000 INFO @ Fri, 10 Dec 2021 12:27:23: 7000000 INFO @ Fri, 10 Dec 2021 12:27:29: 3000000 INFO @ Fri, 10 Dec 2021 12:27:30: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:27:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:27:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:27:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:27:36: 4000000 INFO @ Fri, 10 Dec 2021 12:27:38: 9000000 INFO @ Fri, 10 Dec 2021 12:27:41: 1000000 INFO @ Fri, 10 Dec 2021 12:27:44: 5000000 INFO @ Fri, 10 Dec 2021 12:27:45: 10000000 INFO @ Fri, 10 Dec 2021 12:27:48: 2000000 INFO @ Fri, 10 Dec 2021 12:27:51: 6000000 INFO @ Fri, 10 Dec 2021 12:27:53: 11000000 INFO @ Fri, 10 Dec 2021 12:27:54: 3000000 INFO @ Fri, 10 Dec 2021 12:27:59: 7000000 INFO @ Fri, 10 Dec 2021 12:28:01: 12000000 INFO @ Fri, 10 Dec 2021 12:28:01: 4000000 INFO @ Fri, 10 Dec 2021 12:28:06: 8000000 INFO @ Fri, 10 Dec 2021 12:28:08: 5000000 INFO @ Fri, 10 Dec 2021 12:28:08: 13000000 INFO @ Fri, 10 Dec 2021 12:28:08: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:08: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:08: #1 total tags in treatment: 13028210 INFO @ Fri, 10 Dec 2021 12:28:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:09: #1 tags after filtering in treatment: 13028210 INFO @ Fri, 10 Dec 2021 12:28:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:09: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:09: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:10: #2 number of paired peaks: 85 WARNING @ Fri, 10 Dec 2021 12:28:10: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:14: 9000000 INFO @ Fri, 10 Dec 2021 12:28:14: 6000000 INFO @ Fri, 10 Dec 2021 12:28:21: 7000000 INFO @ Fri, 10 Dec 2021 12:28:21: 10000000 INFO @ Fri, 10 Dec 2021 12:28:28: 8000000 INFO @ Fri, 10 Dec 2021 12:28:29: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:28:34: 9000000 INFO @ Fri, 10 Dec 2021 12:28:36: 12000000 INFO @ Fri, 10 Dec 2021 12:28:41: 10000000 INFO @ Fri, 10 Dec 2021 12:28:44: 13000000 INFO @ Fri, 10 Dec 2021 12:28:44: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:44: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:44: #1 total tags in treatment: 13028210 INFO @ Fri, 10 Dec 2021 12:28:44: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:44: #1 tags after filtering in treatment: 13028210 INFO @ Fri, 10 Dec 2021 12:28:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:44: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:45: #2 number of paired peaks: 85 WARNING @ Fri, 10 Dec 2021 12:28:45: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:28:47: 11000000 INFO @ Fri, 10 Dec 2021 12:28:53: 12000000 INFO @ Fri, 10 Dec 2021 12:28:58: 13000000 INFO @ Fri, 10 Dec 2021 12:28:58: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 12:28:58: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 12:28:58: #1 total tags in treatment: 13028210 INFO @ Fri, 10 Dec 2021 12:28:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:28:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:28:58: #1 tags after filtering in treatment: 13028210 INFO @ Fri, 10 Dec 2021 12:28:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 12:28:58: #1 finished! INFO @ Fri, 10 Dec 2021 12:28:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:28:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:28:59: #2 number of paired peaks: 85 WARNING @ Fri, 10 Dec 2021 12:28:59: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:28:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688942/SRX8688942.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。