Job ID = 14167276 SRX = SRX8688928 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5380319 spots for SRR12174215/SRR12174215.sra Written 5380319 spots for SRR12174215/SRR12174215.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167891 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:02 Multiseed full-index search: 00:01:51 5380319 reads; of these: 5380319 (100.00%) were unpaired; of these: 591261 (10.99%) aligned 0 times 4007764 (74.49%) aligned exactly 1 time 781294 (14.52%) aligned >1 times 89.01% overall alignment rate Time searching: 00:01:54 Overall time: 00:01:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 134779 / 4789058 = 0.0281 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:35:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:35:51: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:35:51: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:35:59: 1000000 INFO @ Fri, 10 Dec 2021 11:36:06: 2000000 INFO @ Fri, 10 Dec 2021 11:36:13: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:36:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:36:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:36:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:36:21: 4000000 INFO @ Fri, 10 Dec 2021 11:36:26: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:36:26: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:36:26: #1 total tags in treatment: 4654279 INFO @ Fri, 10 Dec 2021 11:36:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:36:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:36:26: #1 tags after filtering in treatment: 4654279 INFO @ Fri, 10 Dec 2021 11:36:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:36:26: #1 finished! INFO @ Fri, 10 Dec 2021 11:36:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:36:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:36:27: #2 number of paired peaks: 76 WARNING @ Fri, 10 Dec 2021 11:36:27: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:36:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:36:29: 1000000 INFO @ Fri, 10 Dec 2021 11:36:38: 2000000 BedGraph に変換中... INFO @ Fri, 10 Dec 2021 11:36:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:36:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:36:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:36:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:36:57: 4000000 INFO @ Fri, 10 Dec 2021 11:37:01: 1000000 INFO @ Fri, 10 Dec 2021 11:37:02: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:37:02: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:37:02: #1 total tags in treatment: 4654279 INFO @ Fri, 10 Dec 2021 11:37:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:37:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:37:02: #1 tags after filtering in treatment: 4654279 INFO @ Fri, 10 Dec 2021 11:37:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:37:03: #1 finished! INFO @ Fri, 10 Dec 2021 11:37:03: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:37:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:37:03: #2 number of paired peaks: 76 WARNING @ Fri, 10 Dec 2021 11:37:03: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:37:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:37:09: 2000000 INFO @ Fri, 10 Dec 2021 11:37:17: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:37:25: 4000000 INFO @ Fri, 10 Dec 2021 11:37:31: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 11:37:31: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 11:37:31: #1 total tags in treatment: 4654279 INFO @ Fri, 10 Dec 2021 11:37:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:37:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:37:31: #1 tags after filtering in treatment: 4654279 INFO @ Fri, 10 Dec 2021 11:37:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:37:31: #1 finished! INFO @ Fri, 10 Dec 2021 11:37:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:37:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:37:31: #2 number of paired peaks: 76 WARNING @ Fri, 10 Dec 2021 11:37:31: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:37:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8688928/SRX8688928.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。