Job ID = 9159931 sra ファイルのダウンロード中... Completed: 336639K bytes transferred in 6 seconds (450215K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18285746 spots for /home/okishinya/chipatlas/results/dm3/SRX859014/SRR1779570.sra Written 18285746 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:30 18285746 reads; of these: 18285746 (100.00%) were unpaired; of these: 85907 (0.47%) aligned 0 times 17421893 (95.28%) aligned exactly 1 time 777946 (4.25%) aligned >1 times 99.53% overall alignment rate Time searching: 00:03:30 Overall time: 00:03:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 781 / 18199839 = 0.0000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:17:20: # Command line: callpeak -t SRX859014.bam -f BAM -g dm -n SRX859014.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX859014.05 # format = BAM # ChIP-seq file = ['SRX859014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:17:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:17:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:17:20: # Command line: callpeak -t SRX859014.bam -f BAM -g dm -n SRX859014.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX859014.10 # format = BAM # ChIP-seq file = ['SRX859014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:17:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:17:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:17:20: # Command line: callpeak -t SRX859014.bam -f BAM -g dm -n SRX859014.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX859014.20 # format = BAM # ChIP-seq file = ['SRX859014.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:17:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:17:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:17:27: 1000000 INFO @ Wed, 28 Jun 2017 01:17:27: 1000000 INFO @ Wed, 28 Jun 2017 01:17:27: 1000000 INFO @ Wed, 28 Jun 2017 01:17:34: 2000000 INFO @ Wed, 28 Jun 2017 01:17:34: 2000000 INFO @ Wed, 28 Jun 2017 01:17:34: 2000000 INFO @ Wed, 28 Jun 2017 01:17:41: 3000000 INFO @ Wed, 28 Jun 2017 01:17:41: 3000000 INFO @ Wed, 28 Jun 2017 01:17:41: 3000000 INFO @ Wed, 28 Jun 2017 01:17:48: 4000000 INFO @ Wed, 28 Jun 2017 01:17:48: 4000000 INFO @ Wed, 28 Jun 2017 01:17:48: 4000000 INFO @ Wed, 28 Jun 2017 01:17:54: 5000000 INFO @ Wed, 28 Jun 2017 01:17:54: 5000000 INFO @ Wed, 28 Jun 2017 01:17:54: 5000000 INFO @ Wed, 28 Jun 2017 01:18:01: 6000000 INFO @ Wed, 28 Jun 2017 01:18:01: 6000000 INFO @ Wed, 28 Jun 2017 01:18:01: 6000000 INFO @ Wed, 28 Jun 2017 01:18:08: 7000000 INFO @ Wed, 28 Jun 2017 01:18:08: 7000000 INFO @ Wed, 28 Jun 2017 01:18:08: 7000000 INFO @ Wed, 28 Jun 2017 01:18:14: 8000000 INFO @ Wed, 28 Jun 2017 01:18:14: 8000000 INFO @ Wed, 28 Jun 2017 01:18:15: 8000000 INFO @ Wed, 28 Jun 2017 01:18:21: 9000000 INFO @ Wed, 28 Jun 2017 01:18:21: 9000000 INFO @ Wed, 28 Jun 2017 01:18:21: 9000000 INFO @ Wed, 28 Jun 2017 01:18:28: 10000000 INFO @ Wed, 28 Jun 2017 01:18:28: 10000000 INFO @ Wed, 28 Jun 2017 01:18:28: 10000000 INFO @ Wed, 28 Jun 2017 01:18:35: 11000000 INFO @ Wed, 28 Jun 2017 01:18:35: 11000000 INFO @ Wed, 28 Jun 2017 01:18:35: 11000000 INFO @ Wed, 28 Jun 2017 01:18:42: 12000000 INFO @ Wed, 28 Jun 2017 01:18:42: 12000000 INFO @ Wed, 28 Jun 2017 01:18:42: 12000000 INFO @ Wed, 28 Jun 2017 01:18:49: 13000000 INFO @ Wed, 28 Jun 2017 01:18:49: 13000000 INFO @ Wed, 28 Jun 2017 01:18:49: 13000000 INFO @ Wed, 28 Jun 2017 01:18:56: 14000000 INFO @ Wed, 28 Jun 2017 01:18:56: 14000000 INFO @ Wed, 28 Jun 2017 01:18:57: 14000000 INFO @ Wed, 28 Jun 2017 01:19:03: 15000000 INFO @ Wed, 28 Jun 2017 01:19:03: 15000000 INFO @ Wed, 28 Jun 2017 01:19:04: 15000000 INFO @ Wed, 28 Jun 2017 01:19:10: 16000000 INFO @ Wed, 28 Jun 2017 01:19:10: 16000000 INFO @ Wed, 28 Jun 2017 01:19:11: 16000000 INFO @ Wed, 28 Jun 2017 01:19:17: 17000000 INFO @ Wed, 28 Jun 2017 01:19:17: 17000000 INFO @ Wed, 28 Jun 2017 01:19:18: 17000000 INFO @ Wed, 28 Jun 2017 01:19:24: 18000000 INFO @ Wed, 28 Jun 2017 01:19:24: 18000000 INFO @ Wed, 28 Jun 2017 01:19:25: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:19:25: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:19:25: #1 total tags in treatment: 18199058 INFO @ Wed, 28 Jun 2017 01:19:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:19:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:19:25: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:19:25: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:19:25: #1 total tags in treatment: 18199058 INFO @ Wed, 28 Jun 2017 01:19:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:19:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:19:26: 18000000 INFO @ Wed, 28 Jun 2017 01:19:26: #1 tags after filtering in treatment: 18199058 INFO @ Wed, 28 Jun 2017 01:19:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:19:26: #1 finished! INFO @ Wed, 28 Jun 2017 01:19:26: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:19:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:19:26: #1 tags after filtering in treatment: 18199058 INFO @ Wed, 28 Jun 2017 01:19:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:19:26: #1 finished! INFO @ Wed, 28 Jun 2017 01:19:26: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:19:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:19:27: #2 number of paired peaks: 12 WARNING @ Wed, 28 Jun 2017 01:19:27: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:19:27: Process for pairing-model is terminated! cat: SRX859014.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 01:19:27: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 01:19:27: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 01:19:27: #1 total tags in treatment: 18199058 INFO @ Wed, 28 Jun 2017 01:19:27: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:19:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:19:27: #2 number of paired peaks: 12 WARNING @ Wed, 28 Jun 2017 01:19:27: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:19:27: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX859014.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859014.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859014.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX859014.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX859014.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859014.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859014.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:19:27: #1 tags after filtering in treatment: 18199058 INFO @ Wed, 28 Jun 2017 01:19:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:19:27: #1 finished! INFO @ Wed, 28 Jun 2017 01:19:27: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:19:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:19:28: #2 number of paired peaks: 12 WARNING @ Wed, 28 Jun 2017 01:19:28: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:19:28: Process for pairing-model is terminated! cat: SRX859014.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX859014.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859014.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX859014.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。