Job ID = 14167049 SRX = SRX8574280 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 57863513 spots for SRR12045601/SRR12045601.sra Written 57863513 spots for SRR12045601/SRR12045601.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167499 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:01 57863513 reads; of these: 57863513 (100.00%) were unpaired; of these: 2941435 (5.08%) aligned 0 times 37241023 (64.36%) aligned exactly 1 time 17681055 (30.56%) aligned >1 times 94.92% overall alignment rate Time searching: 00:23:02 Overall time: 00:23:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10713405 / 54922078 = 0.1951 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:03:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:03:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:03:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:03:41: 1000000 INFO @ Fri, 10 Dec 2021 10:03:46: 2000000 INFO @ Fri, 10 Dec 2021 10:03:52: 3000000 INFO @ Fri, 10 Dec 2021 10:03:57: 4000000 INFO @ Fri, 10 Dec 2021 10:04:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:04:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:04:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:04:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:04:07: 6000000 INFO @ Fri, 10 Dec 2021 10:04:12: 1000000 INFO @ Fri, 10 Dec 2021 10:04:12: 7000000 INFO @ Fri, 10 Dec 2021 10:04:17: 2000000 INFO @ Fri, 10 Dec 2021 10:04:18: 8000000 INFO @ Fri, 10 Dec 2021 10:04:22: 3000000 INFO @ Fri, 10 Dec 2021 10:04:23: 9000000 INFO @ Fri, 10 Dec 2021 10:04:28: 4000000 INFO @ Fri, 10 Dec 2021 10:04:29: 10000000 INFO @ Fri, 10 Dec 2021 10:04:33: 5000000 INFO @ Fri, 10 Dec 2021 10:04:34: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:04:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:04:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:04:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:04:39: 6000000 INFO @ Fri, 10 Dec 2021 10:04:40: 12000000 INFO @ Fri, 10 Dec 2021 10:04:43: 1000000 INFO @ Fri, 10 Dec 2021 10:04:44: 7000000 INFO @ Fri, 10 Dec 2021 10:04:45: 13000000 INFO @ Fri, 10 Dec 2021 10:04:49: 2000000 INFO @ Fri, 10 Dec 2021 10:04:50: 8000000 INFO @ Fri, 10 Dec 2021 10:04:51: 14000000 INFO @ Fri, 10 Dec 2021 10:04:56: 9000000 INFO @ Fri, 10 Dec 2021 10:04:56: 3000000 INFO @ Fri, 10 Dec 2021 10:04:56: 15000000 INFO @ Fri, 10 Dec 2021 10:05:01: 10000000 INFO @ Fri, 10 Dec 2021 10:05:02: 16000000 INFO @ Fri, 10 Dec 2021 10:05:02: 4000000 INFO @ Fri, 10 Dec 2021 10:05:07: 11000000 INFO @ Fri, 10 Dec 2021 10:05:08: 17000000 INFO @ Fri, 10 Dec 2021 10:05:09: 5000000 INFO @ Fri, 10 Dec 2021 10:05:13: 12000000 INFO @ Fri, 10 Dec 2021 10:05:13: 18000000 INFO @ Fri, 10 Dec 2021 10:05:16: 6000000 INFO @ Fri, 10 Dec 2021 10:05:18: 13000000 INFO @ Fri, 10 Dec 2021 10:05:19: 19000000 INFO @ Fri, 10 Dec 2021 10:05:22: 7000000 INFO @ Fri, 10 Dec 2021 10:05:24: 14000000 INFO @ Fri, 10 Dec 2021 10:05:25: 20000000 INFO @ Fri, 10 Dec 2021 10:05:29: 8000000 INFO @ Fri, 10 Dec 2021 10:05:30: 15000000 INFO @ Fri, 10 Dec 2021 10:05:30: 21000000 INFO @ Fri, 10 Dec 2021 10:05:35: 16000000 INFO @ Fri, 10 Dec 2021 10:05:35: 9000000 INFO @ Fri, 10 Dec 2021 10:05:36: 22000000 INFO @ Fri, 10 Dec 2021 10:05:41: 17000000 INFO @ Fri, 10 Dec 2021 10:05:41: 23000000 INFO @ Fri, 10 Dec 2021 10:05:42: 10000000 INFO @ Fri, 10 Dec 2021 10:05:46: 18000000 INFO @ Fri, 10 Dec 2021 10:05:47: 24000000 INFO @ Fri, 10 Dec 2021 10:05:49: 11000000 INFO @ Fri, 10 Dec 2021 10:05:52: 19000000 INFO @ Fri, 10 Dec 2021 10:05:53: 25000000 INFO @ Fri, 10 Dec 2021 10:05:55: 12000000 INFO @ Fri, 10 Dec 2021 10:05:58: 20000000 INFO @ Fri, 10 Dec 2021 10:05:58: 26000000 INFO @ Fri, 10 Dec 2021 10:06:02: 13000000 INFO @ Fri, 10 Dec 2021 10:06:03: 21000000 INFO @ Fri, 10 Dec 2021 10:06:04: 27000000 INFO @ Fri, 10 Dec 2021 10:06:08: 14000000 INFO @ Fri, 10 Dec 2021 10:06:09: 22000000 INFO @ Fri, 10 Dec 2021 10:06:09: 28000000 INFO @ Fri, 10 Dec 2021 10:06:15: 15000000 INFO @ Fri, 10 Dec 2021 10:06:15: 23000000 INFO @ Fri, 10 Dec 2021 10:06:15: 29000000 INFO @ Fri, 10 Dec 2021 10:06:20: 24000000 INFO @ Fri, 10 Dec 2021 10:06:20: 30000000 INFO @ Fri, 10 Dec 2021 10:06:21: 16000000 INFO @ Fri, 10 Dec 2021 10:06:26: 31000000 INFO @ Fri, 10 Dec 2021 10:06:26: 25000000 INFO @ Fri, 10 Dec 2021 10:06:28: 17000000 INFO @ Fri, 10 Dec 2021 10:06:31: 32000000 INFO @ Fri, 10 Dec 2021 10:06:31: 26000000 INFO @ Fri, 10 Dec 2021 10:06:35: 18000000 INFO @ Fri, 10 Dec 2021 10:06:37: 33000000 INFO @ Fri, 10 Dec 2021 10:06:37: 27000000 INFO @ Fri, 10 Dec 2021 10:06:41: 19000000 INFO @ Fri, 10 Dec 2021 10:06:42: 34000000 INFO @ Fri, 10 Dec 2021 10:06:42: 28000000 INFO @ Fri, 10 Dec 2021 10:06:48: 35000000 INFO @ Fri, 10 Dec 2021 10:06:48: 20000000 INFO @ Fri, 10 Dec 2021 10:06:48: 29000000 INFO @ Fri, 10 Dec 2021 10:06:53: 36000000 INFO @ Fri, 10 Dec 2021 10:06:53: 30000000 INFO @ Fri, 10 Dec 2021 10:06:54: 21000000 INFO @ Fri, 10 Dec 2021 10:06:59: 37000000 INFO @ Fri, 10 Dec 2021 10:06:59: 31000000 INFO @ Fri, 10 Dec 2021 10:07:01: 22000000 INFO @ Fri, 10 Dec 2021 10:07:04: 38000000 INFO @ Fri, 10 Dec 2021 10:07:04: 32000000 INFO @ Fri, 10 Dec 2021 10:07:07: 23000000 INFO @ Fri, 10 Dec 2021 10:07:10: 39000000 INFO @ Fri, 10 Dec 2021 10:07:10: 33000000 INFO @ Fri, 10 Dec 2021 10:07:14: 24000000 INFO @ Fri, 10 Dec 2021 10:07:15: 40000000 INFO @ Fri, 10 Dec 2021 10:07:15: 34000000 INFO @ Fri, 10 Dec 2021 10:07:20: 25000000 INFO @ Fri, 10 Dec 2021 10:07:21: 41000000 INFO @ Fri, 10 Dec 2021 10:07:21: 35000000 INFO @ Fri, 10 Dec 2021 10:07:26: 42000000 INFO @ Fri, 10 Dec 2021 10:07:26: 36000000 INFO @ Fri, 10 Dec 2021 10:07:27: 26000000 INFO @ Fri, 10 Dec 2021 10:07:32: 43000000 INFO @ Fri, 10 Dec 2021 10:07:32: 37000000 INFO @ Fri, 10 Dec 2021 10:07:34: 27000000 INFO @ Fri, 10 Dec 2021 10:07:37: 44000000 INFO @ Fri, 10 Dec 2021 10:07:37: 38000000 INFO @ Fri, 10 Dec 2021 10:07:39: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:07:39: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:07:39: #1 total tags in treatment: 44208673 INFO @ Fri, 10 Dec 2021 10:07:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:07:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:07:39: #1 tags after filtering in treatment: 44208673 INFO @ Fri, 10 Dec 2021 10:07:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:07:39: #1 finished! INFO @ Fri, 10 Dec 2021 10:07:39: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:07:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:07:40: 28000000 INFO @ Fri, 10 Dec 2021 10:07:42: #2 number of paired peaks: 23 WARNING @ Fri, 10 Dec 2021 10:07:42: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:07:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:07:43: 39000000 INFO @ Fri, 10 Dec 2021 10:07:47: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:07:48: 40000000 INFO @ Fri, 10 Dec 2021 10:07:53: 41000000 INFO @ Fri, 10 Dec 2021 10:07:53: 30000000 INFO @ Fri, 10 Dec 2021 10:07:59: 42000000 INFO @ Fri, 10 Dec 2021 10:07:59: 31000000 INFO @ Fri, 10 Dec 2021 10:08:04: 43000000 INFO @ Fri, 10 Dec 2021 10:08:06: 32000000 INFO @ Fri, 10 Dec 2021 10:08:10: 44000000 INFO @ Fri, 10 Dec 2021 10:08:11: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:08:11: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:08:11: #1 total tags in treatment: 44208673 INFO @ Fri, 10 Dec 2021 10:08:11: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:08:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:08:12: #1 tags after filtering in treatment: 44208673 INFO @ Fri, 10 Dec 2021 10:08:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:08:12: #1 finished! INFO @ Fri, 10 Dec 2021 10:08:12: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:08:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:08:12: 33000000 INFO @ Fri, 10 Dec 2021 10:08:14: #2 number of paired peaks: 23 WARNING @ Fri, 10 Dec 2021 10:08:14: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:08:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:08:18: 34000000 INFO @ Fri, 10 Dec 2021 10:08:24: 35000000 INFO @ Fri, 10 Dec 2021 10:08:30: 36000000 INFO @ Fri, 10 Dec 2021 10:08:36: 37000000 INFO @ Fri, 10 Dec 2021 10:08:42: 38000000 INFO @ Fri, 10 Dec 2021 10:08:49: 39000000 INFO @ Fri, 10 Dec 2021 10:08:55: 40000000 INFO @ Fri, 10 Dec 2021 10:09:01: 41000000 INFO @ Fri, 10 Dec 2021 10:09:07: 42000000 INFO @ Fri, 10 Dec 2021 10:09:13: 43000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:09:20: 44000000 INFO @ Fri, 10 Dec 2021 10:09:21: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:09:21: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:09:21: #1 total tags in treatment: 44208673 INFO @ Fri, 10 Dec 2021 10:09:21: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:09:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:09:22: #1 tags after filtering in treatment: 44208673 INFO @ Fri, 10 Dec 2021 10:09:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:09:22: #1 finished! INFO @ Fri, 10 Dec 2021 10:09:22: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:09:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:09:25: #2 number of paired peaks: 23 WARNING @ Fri, 10 Dec 2021 10:09:25: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:09:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574280/SRX8574280.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling