Job ID = 14167047 SRX = SRX8574278 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 82931494 spots for SRR12045599/SRR12045599.sra Written 82931494 spots for SRR12045599/SRR12045599.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167525 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:32 82931494 reads; of these: 82931494 (100.00%) were unpaired; of these: 3722367 (4.49%) aligned 0 times 54371497 (65.56%) aligned exactly 1 time 24837630 (29.95%) aligned >1 times 95.51% overall alignment rate Time searching: 00:38:32 Overall time: 00:38:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 16913695 / 79209127 = 0.2135 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:24:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:24:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:24:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:25:02: 1000000 INFO @ Fri, 10 Dec 2021 10:25:08: 2000000 INFO @ Fri, 10 Dec 2021 10:25:14: 3000000 INFO @ Fri, 10 Dec 2021 10:25:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:25:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:25:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:25:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:25:26: 5000000 INFO @ Fri, 10 Dec 2021 10:25:32: 1000000 INFO @ Fri, 10 Dec 2021 10:25:32: 6000000 INFO @ Fri, 10 Dec 2021 10:25:38: 2000000 INFO @ Fri, 10 Dec 2021 10:25:39: 7000000 INFO @ Fri, 10 Dec 2021 10:25:45: 3000000 INFO @ Fri, 10 Dec 2021 10:25:45: 8000000 INFO @ Fri, 10 Dec 2021 10:25:51: 4000000 INFO @ Fri, 10 Dec 2021 10:25:51: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:25:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:25:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:25:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:25:57: 5000000 INFO @ Fri, 10 Dec 2021 10:25:57: 10000000 INFO @ Fri, 10 Dec 2021 10:26:03: 1000000 INFO @ Fri, 10 Dec 2021 10:26:04: 6000000 INFO @ Fri, 10 Dec 2021 10:26:04: 11000000 INFO @ Fri, 10 Dec 2021 10:26:09: 2000000 INFO @ Fri, 10 Dec 2021 10:26:10: 7000000 INFO @ Fri, 10 Dec 2021 10:26:10: 12000000 INFO @ Fri, 10 Dec 2021 10:26:16: 3000000 INFO @ Fri, 10 Dec 2021 10:26:17: 13000000 INFO @ Fri, 10 Dec 2021 10:26:17: 8000000 INFO @ Fri, 10 Dec 2021 10:26:22: 4000000 INFO @ Fri, 10 Dec 2021 10:26:23: 14000000 INFO @ Fri, 10 Dec 2021 10:26:23: 9000000 INFO @ Fri, 10 Dec 2021 10:26:29: 5000000 INFO @ Fri, 10 Dec 2021 10:26:29: 15000000 INFO @ Fri, 10 Dec 2021 10:26:29: 10000000 INFO @ Fri, 10 Dec 2021 10:26:35: 6000000 INFO @ Fri, 10 Dec 2021 10:26:35: 16000000 INFO @ Fri, 10 Dec 2021 10:26:36: 11000000 INFO @ Fri, 10 Dec 2021 10:26:41: 7000000 INFO @ Fri, 10 Dec 2021 10:26:41: 17000000 INFO @ Fri, 10 Dec 2021 10:26:42: 12000000 INFO @ Fri, 10 Dec 2021 10:26:47: 18000000 INFO @ Fri, 10 Dec 2021 10:26:47: 8000000 INFO @ Fri, 10 Dec 2021 10:26:48: 13000000 INFO @ Fri, 10 Dec 2021 10:26:54: 19000000 INFO @ Fri, 10 Dec 2021 10:26:54: 9000000 INFO @ Fri, 10 Dec 2021 10:26:54: 14000000 INFO @ Fri, 10 Dec 2021 10:27:00: 20000000 INFO @ Fri, 10 Dec 2021 10:27:00: 10000000 INFO @ Fri, 10 Dec 2021 10:27:00: 15000000 INFO @ Fri, 10 Dec 2021 10:27:06: 21000000 INFO @ Fri, 10 Dec 2021 10:27:06: 11000000 INFO @ Fri, 10 Dec 2021 10:27:07: 16000000 INFO @ Fri, 10 Dec 2021 10:27:12: 22000000 INFO @ Fri, 10 Dec 2021 10:27:13: 12000000 INFO @ Fri, 10 Dec 2021 10:27:13: 17000000 INFO @ Fri, 10 Dec 2021 10:27:18: 23000000 INFO @ Fri, 10 Dec 2021 10:27:19: 13000000 INFO @ Fri, 10 Dec 2021 10:27:19: 18000000 INFO @ Fri, 10 Dec 2021 10:27:25: 24000000 INFO @ Fri, 10 Dec 2021 10:27:26: 14000000 INFO @ Fri, 10 Dec 2021 10:27:26: 19000000 INFO @ Fri, 10 Dec 2021 10:27:31: 25000000 INFO @ Fri, 10 Dec 2021 10:27:32: 15000000 INFO @ Fri, 10 Dec 2021 10:27:32: 20000000 INFO @ Fri, 10 Dec 2021 10:27:37: 26000000 INFO @ Fri, 10 Dec 2021 10:27:38: 16000000 INFO @ Fri, 10 Dec 2021 10:27:38: 21000000 INFO @ Fri, 10 Dec 2021 10:27:43: 27000000 INFO @ Fri, 10 Dec 2021 10:27:44: 17000000 INFO @ Fri, 10 Dec 2021 10:27:45: 22000000 INFO @ Fri, 10 Dec 2021 10:27:50: 28000000 INFO @ Fri, 10 Dec 2021 10:27:51: 18000000 INFO @ Fri, 10 Dec 2021 10:27:51: 23000000 INFO @ Fri, 10 Dec 2021 10:27:56: 29000000 INFO @ Fri, 10 Dec 2021 10:27:57: 19000000 INFO @ Fri, 10 Dec 2021 10:27:57: 24000000 INFO @ Fri, 10 Dec 2021 10:28:02: 30000000 INFO @ Fri, 10 Dec 2021 10:28:03: 20000000 INFO @ Fri, 10 Dec 2021 10:28:03: 25000000 INFO @ Fri, 10 Dec 2021 10:28:08: 31000000 INFO @ Fri, 10 Dec 2021 10:28:09: 21000000 INFO @ Fri, 10 Dec 2021 10:28:09: 26000000 INFO @ Fri, 10 Dec 2021 10:28:14: 32000000 INFO @ Fri, 10 Dec 2021 10:28:15: 22000000 INFO @ Fri, 10 Dec 2021 10:28:15: 27000000 INFO @ Fri, 10 Dec 2021 10:28:20: 33000000 INFO @ Fri, 10 Dec 2021 10:28:21: 23000000 INFO @ Fri, 10 Dec 2021 10:28:22: 28000000 INFO @ Fri, 10 Dec 2021 10:28:27: 34000000 INFO @ Fri, 10 Dec 2021 10:28:28: 24000000 INFO @ Fri, 10 Dec 2021 10:28:28: 29000000 INFO @ Fri, 10 Dec 2021 10:28:33: 35000000 INFO @ Fri, 10 Dec 2021 10:28:34: 25000000 INFO @ Fri, 10 Dec 2021 10:28:34: 30000000 INFO @ Fri, 10 Dec 2021 10:28:39: 36000000 INFO @ Fri, 10 Dec 2021 10:28:40: 26000000 INFO @ Fri, 10 Dec 2021 10:28:40: 31000000 INFO @ Fri, 10 Dec 2021 10:28:45: 37000000 INFO @ Fri, 10 Dec 2021 10:28:46: 27000000 INFO @ Fri, 10 Dec 2021 10:28:47: 32000000 INFO @ Fri, 10 Dec 2021 10:28:51: 38000000 INFO @ Fri, 10 Dec 2021 10:28:53: 28000000 INFO @ Fri, 10 Dec 2021 10:28:53: 33000000 INFO @ Fri, 10 Dec 2021 10:28:58: 39000000 INFO @ Fri, 10 Dec 2021 10:28:59: 29000000 INFO @ Fri, 10 Dec 2021 10:28:59: 34000000 INFO @ Fri, 10 Dec 2021 10:29:04: 40000000 INFO @ Fri, 10 Dec 2021 10:29:05: 30000000 INFO @ Fri, 10 Dec 2021 10:29:05: 35000000 INFO @ Fri, 10 Dec 2021 10:29:10: 41000000 INFO @ Fri, 10 Dec 2021 10:29:11: 31000000 INFO @ Fri, 10 Dec 2021 10:29:11: 36000000 INFO @ Fri, 10 Dec 2021 10:29:16: 42000000 INFO @ Fri, 10 Dec 2021 10:29:17: 32000000 INFO @ Fri, 10 Dec 2021 10:29:17: 37000000 INFO @ Fri, 10 Dec 2021 10:29:22: 43000000 INFO @ Fri, 10 Dec 2021 10:29:23: 33000000 INFO @ Fri, 10 Dec 2021 10:29:23: 38000000 INFO @ Fri, 10 Dec 2021 10:29:28: 44000000 INFO @ Fri, 10 Dec 2021 10:29:29: 34000000 INFO @ Fri, 10 Dec 2021 10:29:29: 39000000 INFO @ Fri, 10 Dec 2021 10:29:35: 45000000 INFO @ Fri, 10 Dec 2021 10:29:35: 35000000 INFO @ Fri, 10 Dec 2021 10:29:36: 40000000 INFO @ Fri, 10 Dec 2021 10:29:41: 46000000 INFO @ Fri, 10 Dec 2021 10:29:41: 36000000 INFO @ Fri, 10 Dec 2021 10:29:42: 41000000 INFO @ Fri, 10 Dec 2021 10:29:47: 47000000 INFO @ Fri, 10 Dec 2021 10:29:48: 37000000 INFO @ Fri, 10 Dec 2021 10:29:48: 42000000 INFO @ Fri, 10 Dec 2021 10:29:53: 48000000 INFO @ Fri, 10 Dec 2021 10:29:54: 38000000 INFO @ Fri, 10 Dec 2021 10:29:54: 43000000 INFO @ Fri, 10 Dec 2021 10:29:59: 49000000 INFO @ Fri, 10 Dec 2021 10:30:00: 39000000 INFO @ Fri, 10 Dec 2021 10:30:00: 44000000 INFO @ Fri, 10 Dec 2021 10:30:05: 50000000 INFO @ Fri, 10 Dec 2021 10:30:06: 40000000 INFO @ Fri, 10 Dec 2021 10:30:06: 45000000 INFO @ Fri, 10 Dec 2021 10:30:12: 51000000 INFO @ Fri, 10 Dec 2021 10:30:12: 41000000 INFO @ Fri, 10 Dec 2021 10:30:12: 46000000 INFO @ Fri, 10 Dec 2021 10:30:18: 52000000 INFO @ Fri, 10 Dec 2021 10:30:18: 42000000 INFO @ Fri, 10 Dec 2021 10:30:19: 47000000 INFO @ Fri, 10 Dec 2021 10:30:24: 53000000 INFO @ Fri, 10 Dec 2021 10:30:24: 43000000 INFO @ Fri, 10 Dec 2021 10:30:25: 48000000 INFO @ Fri, 10 Dec 2021 10:30:30: 54000000 INFO @ Fri, 10 Dec 2021 10:30:30: 44000000 INFO @ Fri, 10 Dec 2021 10:30:31: 49000000 INFO @ Fri, 10 Dec 2021 10:30:36: 55000000 INFO @ Fri, 10 Dec 2021 10:30:36: 45000000 INFO @ Fri, 10 Dec 2021 10:30:37: 50000000 INFO @ Fri, 10 Dec 2021 10:30:42: 56000000 INFO @ Fri, 10 Dec 2021 10:30:43: 46000000 INFO @ Fri, 10 Dec 2021 10:30:43: 51000000 INFO @ Fri, 10 Dec 2021 10:30:49: 57000000 INFO @ Fri, 10 Dec 2021 10:30:49: 47000000 INFO @ Fri, 10 Dec 2021 10:30:49: 52000000 INFO @ Fri, 10 Dec 2021 10:30:55: 58000000 INFO @ Fri, 10 Dec 2021 10:30:55: 48000000 INFO @ Fri, 10 Dec 2021 10:30:56: 53000000 INFO @ Fri, 10 Dec 2021 10:31:01: 59000000 INFO @ Fri, 10 Dec 2021 10:31:01: 49000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:31:02: 54000000 INFO @ Fri, 10 Dec 2021 10:31:07: 60000000 INFO @ Fri, 10 Dec 2021 10:31:07: 50000000 INFO @ Fri, 10 Dec 2021 10:31:09: 55000000 INFO @ Fri, 10 Dec 2021 10:31:13: 61000000 INFO @ Fri, 10 Dec 2021 10:31:14: 51000000 INFO @ Fri, 10 Dec 2021 10:31:15: 56000000 INFO @ Fri, 10 Dec 2021 10:31:20: 62000000 INFO @ Fri, 10 Dec 2021 10:31:20: 52000000 INFO @ Fri, 10 Dec 2021 10:31:21: 57000000 INFO @ Fri, 10 Dec 2021 10:31:22: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:31:22: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:31:22: #1 total tags in treatment: 62295432 INFO @ Fri, 10 Dec 2021 10:31:22: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:31:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:31:23: #1 tags after filtering in treatment: 62295432 INFO @ Fri, 10 Dec 2021 10:31:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:31:23: #1 finished! INFO @ Fri, 10 Dec 2021 10:31:23: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:31:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:31:26: 53000000 INFO @ Fri, 10 Dec 2021 10:31:27: 58000000 INFO @ Fri, 10 Dec 2021 10:31:27: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 10:31:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:31:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:31:33: 54000000 INFO @ Fri, 10 Dec 2021 10:31:33: 59000000 INFO @ Fri, 10 Dec 2021 10:31:39: 55000000 INFO @ Fri, 10 Dec 2021 10:31:40: 60000000 INFO @ Fri, 10 Dec 2021 10:31:45: 56000000 INFO @ Fri, 10 Dec 2021 10:31:46: 61000000 INFO @ Fri, 10 Dec 2021 10:31:51: 57000000 INFO @ Fri, 10 Dec 2021 10:31:52: 62000000 INFO @ Fri, 10 Dec 2021 10:31:54: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:31:54: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:31:54: #1 total tags in treatment: 62295432 INFO @ Fri, 10 Dec 2021 10:31:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:31:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:31:55: #1 tags after filtering in treatment: 62295432 INFO @ Fri, 10 Dec 2021 10:31:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:31:55: #1 finished! INFO @ Fri, 10 Dec 2021 10:31:55: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:31:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:31:58: 58000000 INFO @ Fri, 10 Dec 2021 10:32:00: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 10:32:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:32:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:32:04: 59000000 INFO @ Fri, 10 Dec 2021 10:32:10: 60000000 INFO @ Fri, 10 Dec 2021 10:32:16: 61000000 INFO @ Fri, 10 Dec 2021 10:32:22: 62000000 INFO @ Fri, 10 Dec 2021 10:32:24: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:32:24: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:32:24: #1 total tags in treatment: 62295432 INFO @ Fri, 10 Dec 2021 10:32:24: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:32:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:32:25: #1 tags after filtering in treatment: 62295432 INFO @ Fri, 10 Dec 2021 10:32:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:32:25: #1 finished! INFO @ Fri, 10 Dec 2021 10:32:25: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:32:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:32:30: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 10:32:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:32:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574278/SRX8574278.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。