Job ID = 14167043 SRX = SRX8574270 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 68895741 spots for SRR12045591/SRR12045591.sra Written 68895741 spots for SRR12045591/SRR12045591.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167460 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:43 68895741 reads; of these: 68895741 (100.00%) were unpaired; of these: 5511964 (8.00%) aligned 0 times 44621565 (64.77%) aligned exactly 1 time 18762212 (27.23%) aligned >1 times 92.00% overall alignment rate Time searching: 00:21:43 Overall time: 00:21:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13140292 / 63383777 = 0.2073 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:46:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:46:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:46:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:46:47: 1000000 INFO @ Fri, 10 Dec 2021 09:46:52: 2000000 INFO @ Fri, 10 Dec 2021 09:46:57: 3000000 INFO @ Fri, 10 Dec 2021 09:47:02: 4000000 INFO @ Fri, 10 Dec 2021 09:47:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:47:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:47:11: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:47:11: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:47:13: 6000000 INFO @ Fri, 10 Dec 2021 09:47:17: 1000000 INFO @ Fri, 10 Dec 2021 09:47:18: 7000000 INFO @ Fri, 10 Dec 2021 09:47:23: 2000000 INFO @ Fri, 10 Dec 2021 09:47:24: 8000000 INFO @ Fri, 10 Dec 2021 09:47:29: 3000000 INFO @ Fri, 10 Dec 2021 09:47:30: 9000000 INFO @ Fri, 10 Dec 2021 09:47:34: 4000000 INFO @ Fri, 10 Dec 2021 09:47:35: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:47:40: 5000000 INFO @ Fri, 10 Dec 2021 09:47:41: 11000000 INFO @ Fri, 10 Dec 2021 09:47:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:47:41: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:47:41: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:47:46: 6000000 INFO @ Fri, 10 Dec 2021 09:47:47: 12000000 INFO @ Fri, 10 Dec 2021 09:47:48: 1000000 INFO @ Fri, 10 Dec 2021 09:47:52: 7000000 INFO @ Fri, 10 Dec 2021 09:47:54: 13000000 INFO @ Fri, 10 Dec 2021 09:47:56: 2000000 INFO @ Fri, 10 Dec 2021 09:47:59: 8000000 INFO @ Fri, 10 Dec 2021 09:48:00: 14000000 INFO @ Fri, 10 Dec 2021 09:48:03: 3000000 INFO @ Fri, 10 Dec 2021 09:48:05: 9000000 INFO @ Fri, 10 Dec 2021 09:48:06: 15000000 INFO @ Fri, 10 Dec 2021 09:48:10: 4000000 INFO @ Fri, 10 Dec 2021 09:48:11: 10000000 INFO @ Fri, 10 Dec 2021 09:48:13: 16000000 INFO @ Fri, 10 Dec 2021 09:48:17: 5000000 INFO @ Fri, 10 Dec 2021 09:48:17: 11000000 INFO @ Fri, 10 Dec 2021 09:48:19: 17000000 INFO @ Fri, 10 Dec 2021 09:48:24: 6000000 INFO @ Fri, 10 Dec 2021 09:48:24: 12000000 INFO @ Fri, 10 Dec 2021 09:48:25: 18000000 INFO @ Fri, 10 Dec 2021 09:48:30: 13000000 INFO @ Fri, 10 Dec 2021 09:48:31: 7000000 INFO @ Fri, 10 Dec 2021 09:48:31: 19000000 INFO @ Fri, 10 Dec 2021 09:48:36: 14000000 INFO @ Fri, 10 Dec 2021 09:48:38: 20000000 INFO @ Fri, 10 Dec 2021 09:48:38: 8000000 INFO @ Fri, 10 Dec 2021 09:48:43: 15000000 INFO @ Fri, 10 Dec 2021 09:48:44: 21000000 INFO @ Fri, 10 Dec 2021 09:48:45: 9000000 INFO @ Fri, 10 Dec 2021 09:48:49: 16000000 INFO @ Fri, 10 Dec 2021 09:48:50: 22000000 INFO @ Fri, 10 Dec 2021 09:48:52: 10000000 INFO @ Fri, 10 Dec 2021 09:48:55: 17000000 INFO @ Fri, 10 Dec 2021 09:48:56: 23000000 INFO @ Fri, 10 Dec 2021 09:48:59: 11000000 INFO @ Fri, 10 Dec 2021 09:49:01: 18000000 INFO @ Fri, 10 Dec 2021 09:49:03: 24000000 INFO @ Fri, 10 Dec 2021 09:49:06: 12000000 INFO @ Fri, 10 Dec 2021 09:49:08: 19000000 INFO @ Fri, 10 Dec 2021 09:49:09: 25000000 INFO @ Fri, 10 Dec 2021 09:49:13: 13000000 INFO @ Fri, 10 Dec 2021 09:49:14: 20000000 INFO @ Fri, 10 Dec 2021 09:49:15: 26000000 INFO @ Fri, 10 Dec 2021 09:49:20: 14000000 INFO @ Fri, 10 Dec 2021 09:49:20: 21000000 INFO @ Fri, 10 Dec 2021 09:49:22: 27000000 INFO @ Fri, 10 Dec 2021 09:49:26: 22000000 INFO @ Fri, 10 Dec 2021 09:49:27: 15000000 INFO @ Fri, 10 Dec 2021 09:49:28: 28000000 INFO @ Fri, 10 Dec 2021 09:49:33: 23000000 INFO @ Fri, 10 Dec 2021 09:49:34: 29000000 INFO @ Fri, 10 Dec 2021 09:49:34: 16000000 INFO @ Fri, 10 Dec 2021 09:49:39: 24000000 INFO @ Fri, 10 Dec 2021 09:49:40: 30000000 INFO @ Fri, 10 Dec 2021 09:49:41: 17000000 INFO @ Fri, 10 Dec 2021 09:49:45: 25000000 INFO @ Fri, 10 Dec 2021 09:49:47: 31000000 INFO @ Fri, 10 Dec 2021 09:49:48: 18000000 INFO @ Fri, 10 Dec 2021 09:49:52: 26000000 INFO @ Fri, 10 Dec 2021 09:49:53: 32000000 INFO @ Fri, 10 Dec 2021 09:49:55: 19000000 INFO @ Fri, 10 Dec 2021 09:49:58: 27000000 INFO @ Fri, 10 Dec 2021 09:49:59: 33000000 INFO @ Fri, 10 Dec 2021 09:50:02: 20000000 INFO @ Fri, 10 Dec 2021 09:50:04: 28000000 INFO @ Fri, 10 Dec 2021 09:50:05: 34000000 INFO @ Fri, 10 Dec 2021 09:50:09: 21000000 INFO @ Fri, 10 Dec 2021 09:50:10: 29000000 INFO @ Fri, 10 Dec 2021 09:50:12: 35000000 INFO @ Fri, 10 Dec 2021 09:50:16: 22000000 INFO @ Fri, 10 Dec 2021 09:50:17: 30000000 INFO @ Fri, 10 Dec 2021 09:50:18: 36000000 INFO @ Fri, 10 Dec 2021 09:50:23: 31000000 INFO @ Fri, 10 Dec 2021 09:50:23: 23000000 INFO @ Fri, 10 Dec 2021 09:50:24: 37000000 INFO @ Fri, 10 Dec 2021 09:50:29: 32000000 INFO @ Fri, 10 Dec 2021 09:50:30: 24000000 INFO @ Fri, 10 Dec 2021 09:50:31: 38000000 INFO @ Fri, 10 Dec 2021 09:50:35: 33000000 INFO @ Fri, 10 Dec 2021 09:50:37: 39000000 INFO @ Fri, 10 Dec 2021 09:50:37: 25000000 INFO @ Fri, 10 Dec 2021 09:50:41: 34000000 INFO @ Fri, 10 Dec 2021 09:50:43: 40000000 INFO @ Fri, 10 Dec 2021 09:50:44: 26000000 INFO @ Fri, 10 Dec 2021 09:50:48: 35000000 INFO @ Fri, 10 Dec 2021 09:50:50: 41000000 INFO @ Fri, 10 Dec 2021 09:50:51: 27000000 INFO @ Fri, 10 Dec 2021 09:50:54: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:50:56: 42000000 INFO @ Fri, 10 Dec 2021 09:50:58: 28000000 INFO @ Fri, 10 Dec 2021 09:51:00: 37000000 INFO @ Fri, 10 Dec 2021 09:51:02: 43000000 INFO @ Fri, 10 Dec 2021 09:51:05: 29000000 INFO @ Fri, 10 Dec 2021 09:51:07: 38000000 INFO @ Fri, 10 Dec 2021 09:51:09: 44000000 INFO @ Fri, 10 Dec 2021 09:51:12: 30000000 INFO @ Fri, 10 Dec 2021 09:51:13: 39000000 INFO @ Fri, 10 Dec 2021 09:51:15: 45000000 INFO @ Fri, 10 Dec 2021 09:51:19: 40000000 INFO @ Fri, 10 Dec 2021 09:51:20: 31000000 INFO @ Fri, 10 Dec 2021 09:51:21: 46000000 INFO @ Fri, 10 Dec 2021 09:51:26: 41000000 INFO @ Fri, 10 Dec 2021 09:51:27: 32000000 INFO @ Fri, 10 Dec 2021 09:51:27: 47000000 INFO @ Fri, 10 Dec 2021 09:51:32: 42000000 INFO @ Fri, 10 Dec 2021 09:51:34: 33000000 INFO @ Fri, 10 Dec 2021 09:51:34: 48000000 INFO @ Fri, 10 Dec 2021 09:51:38: 43000000 INFO @ Fri, 10 Dec 2021 09:51:40: 49000000 INFO @ Fri, 10 Dec 2021 09:51:41: 34000000 INFO @ Fri, 10 Dec 2021 09:51:45: 44000000 INFO @ Fri, 10 Dec 2021 09:51:46: 50000000 INFO @ Fri, 10 Dec 2021 09:51:48: 35000000 INFO @ Fri, 10 Dec 2021 09:51:48: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:51:48: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:51:48: #1 total tags in treatment: 50243485 INFO @ Fri, 10 Dec 2021 09:51:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:51:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:51:49: #1 tags after filtering in treatment: 50243485 INFO @ Fri, 10 Dec 2021 09:51:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:51:49: #1 finished! INFO @ Fri, 10 Dec 2021 09:51:49: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:51:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:51:51: 45000000 INFO @ Fri, 10 Dec 2021 09:51:52: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 09:51:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:51:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:51:55: 36000000 INFO @ Fri, 10 Dec 2021 09:51:57: 46000000 INFO @ Fri, 10 Dec 2021 09:52:02: 37000000 INFO @ Fri, 10 Dec 2021 09:52:03: 47000000 INFO @ Fri, 10 Dec 2021 09:52:09: 38000000 INFO @ Fri, 10 Dec 2021 09:52:09: 48000000 INFO @ Fri, 10 Dec 2021 09:52:16: 49000000 INFO @ Fri, 10 Dec 2021 09:52:16: 39000000 INFO @ Fri, 10 Dec 2021 09:52:22: 50000000 INFO @ Fri, 10 Dec 2021 09:52:23: 40000000 INFO @ Fri, 10 Dec 2021 09:52:23: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:52:23: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:52:23: #1 total tags in treatment: 50243485 INFO @ Fri, 10 Dec 2021 09:52:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:52:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:52:24: #1 tags after filtering in treatment: 50243485 INFO @ Fri, 10 Dec 2021 09:52:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:52:24: #1 finished! INFO @ Fri, 10 Dec 2021 09:52:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:52:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:52:27: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 09:52:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:52:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 09:52:30: 41000000 INFO @ Fri, 10 Dec 2021 09:52:36: 42000000 INFO @ Fri, 10 Dec 2021 09:52:43: 43000000 INFO @ Fri, 10 Dec 2021 09:52:49: 44000000 INFO @ Fri, 10 Dec 2021 09:52:56: 45000000 INFO @ Fri, 10 Dec 2021 09:53:02: 46000000 INFO @ Fri, 10 Dec 2021 09:53:09: 47000000 INFO @ Fri, 10 Dec 2021 09:53:15: 48000000 INFO @ Fri, 10 Dec 2021 09:53:22: 49000000 INFO @ Fri, 10 Dec 2021 09:53:28: 50000000 INFO @ Fri, 10 Dec 2021 09:53:30: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:53:30: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:53:30: #1 total tags in treatment: 50243485 INFO @ Fri, 10 Dec 2021 09:53:30: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:53:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:53:31: #1 tags after filtering in treatment: 50243485 INFO @ Fri, 10 Dec 2021 09:53:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:53:31: #1 finished! INFO @ Fri, 10 Dec 2021 09:53:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:53:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:53:34: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 09:53:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:53:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574270/SRX8574270.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling