Job ID = 14167031 SRX = SRX8574266 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27653315 spots for SRR12045587/SRR12045587.sra Written 27653315 spots for SRR12045587/SRR12045587.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167395 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:11 27653315 reads; of these: 27653315 (100.00%) were unpaired; of these: 1090213 (3.94%) aligned 0 times 19085112 (69.02%) aligned exactly 1 time 7477990 (27.04%) aligned >1 times 96.06% overall alignment rate Time searching: 00:10:11 Overall time: 00:10:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3636202 / 26563102 = 0.1369 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:16:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:16:05: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:16:05: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:16:10: 1000000 INFO @ Fri, 10 Dec 2021 09:16:15: 2000000 INFO @ Fri, 10 Dec 2021 09:16:19: 3000000 INFO @ Fri, 10 Dec 2021 09:16:24: 4000000 INFO @ Fri, 10 Dec 2021 09:16:28: 5000000 INFO @ Fri, 10 Dec 2021 09:16:33: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:16:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:16:35: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:16:35: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:16:38: 7000000 INFO @ Fri, 10 Dec 2021 09:16:41: 1000000 INFO @ Fri, 10 Dec 2021 09:16:42: 8000000 INFO @ Fri, 10 Dec 2021 09:16:46: 2000000 INFO @ Fri, 10 Dec 2021 09:16:47: 9000000 INFO @ Fri, 10 Dec 2021 09:16:52: 3000000 INFO @ Fri, 10 Dec 2021 09:16:52: 10000000 INFO @ Fri, 10 Dec 2021 09:16:57: 11000000 INFO @ Fri, 10 Dec 2021 09:16:57: 4000000 INFO @ Fri, 10 Dec 2021 09:17:02: 12000000 INFO @ Fri, 10 Dec 2021 09:17:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:17:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:17:05: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:17:05: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:17:07: 13000000 INFO @ Fri, 10 Dec 2021 09:17:09: 6000000 INFO @ Fri, 10 Dec 2021 09:17:10: 1000000 INFO @ Fri, 10 Dec 2021 09:17:12: 14000000 INFO @ Fri, 10 Dec 2021 09:17:14: 7000000 INFO @ Fri, 10 Dec 2021 09:17:15: 2000000 INFO @ Fri, 10 Dec 2021 09:17:16: 15000000 INFO @ Fri, 10 Dec 2021 09:17:20: 8000000 INFO @ Fri, 10 Dec 2021 09:17:20: 3000000 INFO @ Fri, 10 Dec 2021 09:17:21: 16000000 INFO @ Fri, 10 Dec 2021 09:17:25: 4000000 INFO @ Fri, 10 Dec 2021 09:17:25: 9000000 INFO @ Fri, 10 Dec 2021 09:17:26: 17000000 INFO @ Fri, 10 Dec 2021 09:17:30: 5000000 INFO @ Fri, 10 Dec 2021 09:17:31: 10000000 INFO @ Fri, 10 Dec 2021 09:17:31: 18000000 INFO @ Fri, 10 Dec 2021 09:17:35: 6000000 INFO @ Fri, 10 Dec 2021 09:17:36: 19000000 INFO @ Fri, 10 Dec 2021 09:17:37: 11000000 INFO @ Fri, 10 Dec 2021 09:17:40: 7000000 INFO @ Fri, 10 Dec 2021 09:17:41: 20000000 INFO @ Fri, 10 Dec 2021 09:17:42: 12000000 INFO @ Fri, 10 Dec 2021 09:17:45: 8000000 INFO @ Fri, 10 Dec 2021 09:17:46: 21000000 INFO @ Fri, 10 Dec 2021 09:17:48: 13000000 INFO @ Fri, 10 Dec 2021 09:17:50: 9000000 INFO @ Fri, 10 Dec 2021 09:17:51: 22000000 INFO @ Fri, 10 Dec 2021 09:17:53: 14000000 INFO @ Fri, 10 Dec 2021 09:17:55: 10000000 INFO @ Fri, 10 Dec 2021 09:17:56: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:17:56: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:17:56: #1 total tags in treatment: 22926900 INFO @ Fri, 10 Dec 2021 09:17:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:17:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:17:57: #1 tags after filtering in treatment: 22926900 INFO @ Fri, 10 Dec 2021 09:17:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:17:57: #1 finished! INFO @ Fri, 10 Dec 2021 09:17:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:17:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:17:58: #2 number of paired peaks: 61 WARNING @ Fri, 10 Dec 2021 09:17:58: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:17:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:17:59: 15000000 INFO @ Fri, 10 Dec 2021 09:18:00: 11000000 INFO @ Fri, 10 Dec 2021 09:18:05: 16000000 INFO @ Fri, 10 Dec 2021 09:18:05: 12000000 INFO @ Fri, 10 Dec 2021 09:18:10: 13000000 INFO @ Fri, 10 Dec 2021 09:18:10: 17000000 INFO @ Fri, 10 Dec 2021 09:18:15: 14000000 INFO @ Fri, 10 Dec 2021 09:18:16: 18000000 INFO @ Fri, 10 Dec 2021 09:18:19: 15000000 INFO @ Fri, 10 Dec 2021 09:18:21: 19000000 INFO @ Fri, 10 Dec 2021 09:18:24: 16000000 INFO @ Fri, 10 Dec 2021 09:18:27: 20000000 INFO @ Fri, 10 Dec 2021 09:18:29: 17000000 INFO @ Fri, 10 Dec 2021 09:18:32: 21000000 INFO @ Fri, 10 Dec 2021 09:18:34: 18000000 INFO @ Fri, 10 Dec 2021 09:18:38: 22000000 INFO @ Fri, 10 Dec 2021 09:18:39: 19000000 INFO @ Fri, 10 Dec 2021 09:18:43: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:18:43: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:18:43: #1 total tags in treatment: 22926900 INFO @ Fri, 10 Dec 2021 09:18:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:18:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:18:44: 20000000 INFO @ Fri, 10 Dec 2021 09:18:44: #1 tags after filtering in treatment: 22926900 INFO @ Fri, 10 Dec 2021 09:18:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:18:44: #1 finished! INFO @ Fri, 10 Dec 2021 09:18:44: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:18:44: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:18:45: #2 number of paired peaks: 61 WARNING @ Fri, 10 Dec 2021 09:18:45: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:18:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:18:48: 21000000 INFO @ Fri, 10 Dec 2021 09:18:53: 22000000 INFO @ Fri, 10 Dec 2021 09:18:58: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:18:58: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:18:58: #1 total tags in treatment: 22926900 INFO @ Fri, 10 Dec 2021 09:18:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:18:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:18:58: #1 tags after filtering in treatment: 22926900 INFO @ Fri, 10 Dec 2021 09:18:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:18:58: #1 finished! INFO @ Fri, 10 Dec 2021 09:18:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:18:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:19:00: #2 number of paired peaks: 61 WARNING @ Fri, 10 Dec 2021 09:19:00: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:19:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574266/SRX8574266.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。