Job ID = 14167029 SRX = SRX8574264 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 66766333 spots for SRR12045585/SRR12045585.sra Written 66766333 spots for SRR12045585/SRR12045585.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167409 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:00 66766333 reads; of these: 66766333 (100.00%) were unpaired; of these: 2241698 (3.36%) aligned 0 times 45806771 (68.61%) aligned exactly 1 time 18717864 (28.03%) aligned >1 times 96.64% overall alignment rate Time searching: 00:21:00 Overall time: 00:21:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13375778 / 64524635 = 0.2073 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:28:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:28:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:28:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:28:51: 1000000 INFO @ Fri, 10 Dec 2021 09:28:56: 2000000 INFO @ Fri, 10 Dec 2021 09:29:01: 3000000 INFO @ Fri, 10 Dec 2021 09:29:05: 4000000 INFO @ Fri, 10 Dec 2021 09:29:10: 5000000 INFO @ Fri, 10 Dec 2021 09:29:15: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:29:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:29:17: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:29:17: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:29:20: 7000000 INFO @ Fri, 10 Dec 2021 09:29:22: 1000000 INFO @ Fri, 10 Dec 2021 09:29:25: 8000000 INFO @ Fri, 10 Dec 2021 09:29:27: 2000000 INFO @ Fri, 10 Dec 2021 09:29:29: 9000000 INFO @ Fri, 10 Dec 2021 09:29:32: 3000000 INFO @ Fri, 10 Dec 2021 09:29:34: 10000000 INFO @ Fri, 10 Dec 2021 09:29:37: 4000000 INFO @ Fri, 10 Dec 2021 09:29:39: 11000000 INFO @ Fri, 10 Dec 2021 09:29:42: 5000000 INFO @ Fri, 10 Dec 2021 09:29:44: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:29:47: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:29:47: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:29:47: 6000000 INFO @ Fri, 10 Dec 2021 09:29:49: 13000000 INFO @ Fri, 10 Dec 2021 09:29:52: 7000000 INFO @ Fri, 10 Dec 2021 09:29:53: 1000000 INFO @ Fri, 10 Dec 2021 09:29:54: 14000000 INFO @ Fri, 10 Dec 2021 09:29:57: 8000000 INFO @ Fri, 10 Dec 2021 09:29:58: 2000000 INFO @ Fri, 10 Dec 2021 09:29:59: 15000000 INFO @ Fri, 10 Dec 2021 09:30:03: 9000000 INFO @ Fri, 10 Dec 2021 09:30:04: 3000000 INFO @ Fri, 10 Dec 2021 09:30:04: 16000000 INFO @ Fri, 10 Dec 2021 09:30:08: 10000000 INFO @ Fri, 10 Dec 2021 09:30:09: 4000000 INFO @ Fri, 10 Dec 2021 09:30:09: 17000000 INFO @ Fri, 10 Dec 2021 09:30:13: 11000000 INFO @ Fri, 10 Dec 2021 09:30:14: 18000000 INFO @ Fri, 10 Dec 2021 09:30:15: 5000000 INFO @ Fri, 10 Dec 2021 09:30:18: 12000000 INFO @ Fri, 10 Dec 2021 09:30:19: 19000000 INFO @ Fri, 10 Dec 2021 09:30:20: 6000000 INFO @ Fri, 10 Dec 2021 09:30:23: 13000000 INFO @ Fri, 10 Dec 2021 09:30:24: 20000000 INFO @ Fri, 10 Dec 2021 09:30:26: 7000000 INFO @ Fri, 10 Dec 2021 09:30:28: 14000000 INFO @ Fri, 10 Dec 2021 09:30:29: 21000000 INFO @ Fri, 10 Dec 2021 09:30:32: 8000000 INFO @ Fri, 10 Dec 2021 09:30:33: 15000000 INFO @ Fri, 10 Dec 2021 09:30:34: 22000000 INFO @ Fri, 10 Dec 2021 09:30:37: 9000000 INFO @ Fri, 10 Dec 2021 09:30:38: 16000000 INFO @ Fri, 10 Dec 2021 09:30:39: 23000000 INFO @ Fri, 10 Dec 2021 09:30:43: 10000000 INFO @ Fri, 10 Dec 2021 09:30:44: 17000000 INFO @ Fri, 10 Dec 2021 09:30:44: 24000000 INFO @ Fri, 10 Dec 2021 09:30:48: 11000000 INFO @ Fri, 10 Dec 2021 09:30:49: 18000000 INFO @ Fri, 10 Dec 2021 09:30:49: 25000000 INFO @ Fri, 10 Dec 2021 09:30:54: 19000000 INFO @ Fri, 10 Dec 2021 09:30:54: 12000000 INFO @ Fri, 10 Dec 2021 09:30:54: 26000000 INFO @ Fri, 10 Dec 2021 09:30:59: 20000000 INFO @ Fri, 10 Dec 2021 09:30:59: 27000000 INFO @ Fri, 10 Dec 2021 09:31:00: 13000000 INFO @ Fri, 10 Dec 2021 09:31:04: 21000000 INFO @ Fri, 10 Dec 2021 09:31:04: 28000000 INFO @ Fri, 10 Dec 2021 09:31:05: 14000000 INFO @ Fri, 10 Dec 2021 09:31:09: 29000000 INFO @ Fri, 10 Dec 2021 09:31:09: 22000000 INFO @ Fri, 10 Dec 2021 09:31:11: 15000000 INFO @ Fri, 10 Dec 2021 09:31:14: 30000000 INFO @ Fri, 10 Dec 2021 09:31:14: 23000000 INFO @ Fri, 10 Dec 2021 09:31:16: 16000000 INFO @ Fri, 10 Dec 2021 09:31:19: 31000000 INFO @ Fri, 10 Dec 2021 09:31:19: 24000000 INFO @ Fri, 10 Dec 2021 09:31:22: 17000000 INFO @ Fri, 10 Dec 2021 09:31:24: 32000000 INFO @ Fri, 10 Dec 2021 09:31:24: 25000000 INFO @ Fri, 10 Dec 2021 09:31:29: 18000000 INFO @ Fri, 10 Dec 2021 09:31:29: 33000000 INFO @ Fri, 10 Dec 2021 09:31:30: 26000000 INFO @ Fri, 10 Dec 2021 09:31:34: 34000000 INFO @ Fri, 10 Dec 2021 09:31:35: 27000000 INFO @ Fri, 10 Dec 2021 09:31:36: 19000000 INFO @ Fri, 10 Dec 2021 09:31:39: 35000000 INFO @ Fri, 10 Dec 2021 09:31:40: 28000000 INFO @ Fri, 10 Dec 2021 09:31:42: 20000000 INFO @ Fri, 10 Dec 2021 09:31:44: 36000000 INFO @ Fri, 10 Dec 2021 09:31:45: 29000000 INFO @ Fri, 10 Dec 2021 09:31:49: 37000000 INFO @ Fri, 10 Dec 2021 09:31:49: 21000000 INFO @ Fri, 10 Dec 2021 09:31:50: 30000000 INFO @ Fri, 10 Dec 2021 09:31:54: 38000000 INFO @ Fri, 10 Dec 2021 09:31:55: 31000000 INFO @ Fri, 10 Dec 2021 09:31:56: 22000000 INFO @ Fri, 10 Dec 2021 09:31:59: 39000000 INFO @ Fri, 10 Dec 2021 09:32:00: 32000000 INFO @ Fri, 10 Dec 2021 09:32:02: 23000000 INFO @ Fri, 10 Dec 2021 09:32:04: 40000000 INFO @ Fri, 10 Dec 2021 09:32:05: 33000000 INFO @ Fri, 10 Dec 2021 09:32:08: 24000000 INFO @ Fri, 10 Dec 2021 09:32:09: 41000000 INFO @ Fri, 10 Dec 2021 09:32:10: 34000000 INFO @ Fri, 10 Dec 2021 09:32:13: 42000000 INFO @ Fri, 10 Dec 2021 09:32:14: 25000000 INFO @ Fri, 10 Dec 2021 09:32:15: 35000000 INFO @ Fri, 10 Dec 2021 09:32:18: 43000000 INFO @ Fri, 10 Dec 2021 09:32:19: 26000000 INFO @ Fri, 10 Dec 2021 09:32:20: 36000000 INFO @ Fri, 10 Dec 2021 09:32:23: 44000000 INFO @ Fri, 10 Dec 2021 09:32:25: 27000000 INFO @ Fri, 10 Dec 2021 09:32:25: 37000000 INFO @ Fri, 10 Dec 2021 09:32:28: 45000000 INFO @ Fri, 10 Dec 2021 09:32:30: 38000000 INFO @ Fri, 10 Dec 2021 09:32:31: 28000000 INFO @ Fri, 10 Dec 2021 09:32:33: 46000000 INFO @ Fri, 10 Dec 2021 09:32:35: 39000000 INFO @ Fri, 10 Dec 2021 09:32:36: 29000000 INFO @ Fri, 10 Dec 2021 09:32:38: 47000000 INFO @ Fri, 10 Dec 2021 09:32:40: 40000000 INFO @ Fri, 10 Dec 2021 09:32:42: 30000000 INFO @ Fri, 10 Dec 2021 09:32:43: 48000000 INFO @ Fri, 10 Dec 2021 09:32:45: 41000000 INFO @ Fri, 10 Dec 2021 09:32:47: 31000000 INFO @ Fri, 10 Dec 2021 09:32:48: 49000000 INFO @ Fri, 10 Dec 2021 09:32:50: 42000000 INFO @ Fri, 10 Dec 2021 09:32:53: 32000000 INFO @ Fri, 10 Dec 2021 09:32:53: 50000000 INFO @ Fri, 10 Dec 2021 09:32:55: 43000000 INFO @ Fri, 10 Dec 2021 09:32:58: 51000000 INFO @ Fri, 10 Dec 2021 09:32:58: 33000000 INFO @ Fri, 10 Dec 2021 09:32:59: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:32:59: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:32:59: #1 total tags in treatment: 51148857 INFO @ Fri, 10 Dec 2021 09:32:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:32:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:33:00: #1 tags after filtering in treatment: 51148857 INFO @ Fri, 10 Dec 2021 09:33:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:33:00: #1 finished! INFO @ Fri, 10 Dec 2021 09:33:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:33:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:33:00: 44000000 INFO @ Fri, 10 Dec 2021 09:33:03: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 09:33:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:33:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:33:04: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:33:05: 45000000 INFO @ Fri, 10 Dec 2021 09:33:09: 35000000 INFO @ Fri, 10 Dec 2021 09:33:10: 46000000 INFO @ Fri, 10 Dec 2021 09:33:15: 47000000 INFO @ Fri, 10 Dec 2021 09:33:15: 36000000 INFO @ Fri, 10 Dec 2021 09:33:20: 48000000 INFO @ Fri, 10 Dec 2021 09:33:21: 37000000 INFO @ Fri, 10 Dec 2021 09:33:25: 49000000 INFO @ Fri, 10 Dec 2021 09:33:27: 38000000 INFO @ Fri, 10 Dec 2021 09:33:30: 50000000 INFO @ Fri, 10 Dec 2021 09:33:33: 39000000 INFO @ Fri, 10 Dec 2021 09:33:35: 51000000 INFO @ Fri, 10 Dec 2021 09:33:36: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:33:36: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:33:36: #1 total tags in treatment: 51148857 INFO @ Fri, 10 Dec 2021 09:33:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:33:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:33:37: #1 tags after filtering in treatment: 51148857 INFO @ Fri, 10 Dec 2021 09:33:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:33:37: #1 finished! INFO @ Fri, 10 Dec 2021 09:33:37: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:33:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:33:39: 40000000 INFO @ Fri, 10 Dec 2021 09:33:40: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 09:33:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:33:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:33:45: 41000000 INFO @ Fri, 10 Dec 2021 09:33:51: 42000000 INFO @ Fri, 10 Dec 2021 09:33:56: 43000000 INFO @ Fri, 10 Dec 2021 09:34:01: 44000000 INFO @ Fri, 10 Dec 2021 09:34:07: 45000000 INFO @ Fri, 10 Dec 2021 09:34:13: 46000000 INFO @ Fri, 10 Dec 2021 09:34:19: 47000000 INFO @ Fri, 10 Dec 2021 09:34:25: 48000000 INFO @ Fri, 10 Dec 2021 09:34:31: 49000000 INFO @ Fri, 10 Dec 2021 09:34:38: 50000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 09:34:45: 51000000 INFO @ Fri, 10 Dec 2021 09:34:46: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:34:46: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:34:46: #1 total tags in treatment: 51148857 INFO @ Fri, 10 Dec 2021 09:34:46: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:34:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:34:47: #1 tags after filtering in treatment: 51148857 INFO @ Fri, 10 Dec 2021 09:34:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:34:47: #1 finished! INFO @ Fri, 10 Dec 2021 09:34:47: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:34:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:34:50: #2 number of paired peaks: 0 WARNING @ Fri, 10 Dec 2021 09:34:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:34:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574264/SRX8574264.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling