Job ID = 14167027 SRX = SRX8574262 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39426069 spots for SRR12045583/SRR12045583.sra Written 39426069 spots for SRR12045583/SRR12045583.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167390 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:28 39426069 reads; of these: 39426069 (100.00%) were unpaired; of these: 3368354 (8.54%) aligned 0 times 19139026 (48.54%) aligned exactly 1 time 16918689 (42.91%) aligned >1 times 91.46% overall alignment rate Time searching: 00:12:28 Overall time: 00:12:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13307443 / 36057715 = 0.3691 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:14:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:14:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:14:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:14:36: 1000000 INFO @ Fri, 10 Dec 2021 09:14:42: 2000000 INFO @ Fri, 10 Dec 2021 09:14:48: 3000000 INFO @ Fri, 10 Dec 2021 09:14:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:14:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:14:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:14:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:15:01: 5000000 INFO @ Fri, 10 Dec 2021 09:15:06: 1000000 INFO @ Fri, 10 Dec 2021 09:15:08: 6000000 INFO @ Fri, 10 Dec 2021 09:15:13: 2000000 INFO @ Fri, 10 Dec 2021 09:15:15: 7000000 INFO @ Fri, 10 Dec 2021 09:15:20: 3000000 INFO @ Fri, 10 Dec 2021 09:15:22: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 09:15:28: 4000000 INFO @ Fri, 10 Dec 2021 09:15:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 09:15:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 09:15:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 09:15:30: 9000000 INFO @ Fri, 10 Dec 2021 09:15:35: 5000000 INFO @ Fri, 10 Dec 2021 09:15:37: 1000000 INFO @ Fri, 10 Dec 2021 09:15:37: 10000000 INFO @ Fri, 10 Dec 2021 09:15:42: 6000000 INFO @ Fri, 10 Dec 2021 09:15:44: 2000000 INFO @ Fri, 10 Dec 2021 09:15:44: 11000000 INFO @ Fri, 10 Dec 2021 09:15:50: 7000000 INFO @ Fri, 10 Dec 2021 09:15:51: 3000000 INFO @ Fri, 10 Dec 2021 09:15:51: 12000000 INFO @ Fri, 10 Dec 2021 09:15:57: 8000000 INFO @ Fri, 10 Dec 2021 09:15:58: 4000000 INFO @ Fri, 10 Dec 2021 09:15:59: 13000000 INFO @ Fri, 10 Dec 2021 09:16:04: 9000000 INFO @ Fri, 10 Dec 2021 09:16:06: 5000000 INFO @ Fri, 10 Dec 2021 09:16:06: 14000000 INFO @ Fri, 10 Dec 2021 09:16:11: 10000000 INFO @ Fri, 10 Dec 2021 09:16:13: 6000000 INFO @ Fri, 10 Dec 2021 09:16:13: 15000000 INFO @ Fri, 10 Dec 2021 09:16:18: 11000000 INFO @ Fri, 10 Dec 2021 09:16:20: 7000000 INFO @ Fri, 10 Dec 2021 09:16:20: 16000000 INFO @ Fri, 10 Dec 2021 09:16:25: 12000000 INFO @ Fri, 10 Dec 2021 09:16:27: 8000000 INFO @ Fri, 10 Dec 2021 09:16:27: 17000000 INFO @ Fri, 10 Dec 2021 09:16:32: 13000000 INFO @ Fri, 10 Dec 2021 09:16:34: 9000000 INFO @ Fri, 10 Dec 2021 09:16:34: 18000000 INFO @ Fri, 10 Dec 2021 09:16:40: 14000000 INFO @ Fri, 10 Dec 2021 09:16:41: 10000000 INFO @ Fri, 10 Dec 2021 09:16:41: 19000000 INFO @ Fri, 10 Dec 2021 09:16:47: 15000000 INFO @ Fri, 10 Dec 2021 09:16:48: 11000000 INFO @ Fri, 10 Dec 2021 09:16:49: 20000000 INFO @ Fri, 10 Dec 2021 09:16:54: 16000000 INFO @ Fri, 10 Dec 2021 09:16:56: 12000000 INFO @ Fri, 10 Dec 2021 09:16:56: 21000000 INFO @ Fri, 10 Dec 2021 09:17:01: 17000000 INFO @ Fri, 10 Dec 2021 09:17:03: 13000000 INFO @ Fri, 10 Dec 2021 09:17:03: 22000000 INFO @ Fri, 10 Dec 2021 09:17:08: 18000000 INFO @ Fri, 10 Dec 2021 09:17:09: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:17:09: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:17:09: #1 total tags in treatment: 22750272 INFO @ Fri, 10 Dec 2021 09:17:09: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:17:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:17:09: #1 tags after filtering in treatment: 22750272 INFO @ Fri, 10 Dec 2021 09:17:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:17:09: #1 finished! INFO @ Fri, 10 Dec 2021 09:17:09: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:17:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:17:10: 14000000 INFO @ Fri, 10 Dec 2021 09:17:11: #2 number of paired peaks: 79 WARNING @ Fri, 10 Dec 2021 09:17:11: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:17:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:17:16: 19000000 INFO @ Fri, 10 Dec 2021 09:17:17: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 09:17:23: 20000000 INFO @ Fri, 10 Dec 2021 09:17:24: 16000000 INFO @ Fri, 10 Dec 2021 09:17:30: 21000000 INFO @ Fri, 10 Dec 2021 09:17:31: 17000000 INFO @ Fri, 10 Dec 2021 09:17:37: 22000000 INFO @ Fri, 10 Dec 2021 09:17:38: 18000000 INFO @ Fri, 10 Dec 2021 09:17:42: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:17:42: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:17:42: #1 total tags in treatment: 22750272 INFO @ Fri, 10 Dec 2021 09:17:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:17:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:17:42: #1 tags after filtering in treatment: 22750272 INFO @ Fri, 10 Dec 2021 09:17:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:17:42: #1 finished! INFO @ Fri, 10 Dec 2021 09:17:42: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:17:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:17:44: #2 number of paired peaks: 79 WARNING @ Fri, 10 Dec 2021 09:17:44: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:17:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 09:17:45: 19000000 INFO @ Fri, 10 Dec 2021 09:17:51: 20000000 INFO @ Fri, 10 Dec 2021 09:17:58: 21000000 INFO @ Fri, 10 Dec 2021 09:18:05: 22000000 INFO @ Fri, 10 Dec 2021 09:18:10: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 09:18:10: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 09:18:10: #1 total tags in treatment: 22750272 INFO @ Fri, 10 Dec 2021 09:18:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 09:18:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 09:18:10: #1 tags after filtering in treatment: 22750272 INFO @ Fri, 10 Dec 2021 09:18:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 09:18:10: #1 finished! INFO @ Fri, 10 Dec 2021 09:18:10: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 09:18:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 09:18:12: #2 number of paired peaks: 79 WARNING @ Fri, 10 Dec 2021 09:18:12: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 09:18:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8574262/SRX8574262.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。