Job ID = 14170269 SRX = SRX8556391 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13545090 spots for SRR12024947/SRR12024947.sra Written 13545090 spots for SRR12024947/SRR12024947.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170746 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:54 13545090 reads; of these: 13545090 (100.00%) were paired; of these: 11959802 (88.30%) aligned concordantly 0 times 503820 (3.72%) aligned concordantly exactly 1 time 1081468 (7.98%) aligned concordantly >1 times ---- 11959802 pairs aligned concordantly 0 times; of these: 191341 (1.60%) aligned discordantly 1 time ---- 11768461 pairs aligned 0 times concordantly or discordantly; of these: 23536922 mates make up the pairs; of these: 22280899 (94.66%) aligned 0 times 287661 (1.22%) aligned exactly 1 time 968362 (4.11%) aligned >1 times 17.75% overall alignment rate Time searching: 00:20:54 Overall time: 00:20:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 757509 / 1728033 = 0.4384 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:25:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:25:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:25:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:25:27: 1000000 INFO @ Sat, 11 Dec 2021 06:25:35: 2000000 INFO @ Sat, 11 Dec 2021 06:25:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:25:47: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 06:25:47: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 06:25:47: #1 total tags in treatment: 888364 INFO @ Sat, 11 Dec 2021 06:25:47: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:25:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:25:47: #1 tags after filtering in treatment: 625710 INFO @ Sat, 11 Dec 2021 06:25:47: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 11 Dec 2021 06:25:47: #1 finished! INFO @ Sat, 11 Dec 2021 06:25:47: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:25:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:25:47: #2 number of paired peaks: 3084 INFO @ Sat, 11 Dec 2021 06:25:47: start model_add_line... INFO @ Sat, 11 Dec 2021 06:25:47: start X-correlation... INFO @ Sat, 11 Dec 2021 06:25:47: end of X-cor INFO @ Sat, 11 Dec 2021 06:25:47: #2 finished! INFO @ Sat, 11 Dec 2021 06:25:47: #2 predicted fragment length is 169 bps INFO @ Sat, 11 Dec 2021 06:25:47: #2 alternative fragment length(s) may be 169 bps INFO @ Sat, 11 Dec 2021 06:25:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.05_model.r WARNING @ Sat, 11 Dec 2021 06:25:47: #2 Since the d (169) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:25:47: #2 You may need to consider one of the other alternative d(s): 169 WARNING @ Sat, 11 Dec 2021 06:25:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:25:47: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:25:47: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:25:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:25:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:25:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:25:49: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:25:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.05_peaks.xls INFO @ Sat, 11 Dec 2021 06:25:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:25:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.05_summits.bed INFO @ Sat, 11 Dec 2021 06:25:50: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1294 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:25:55: 1000000 INFO @ Sat, 11 Dec 2021 06:26:02: 2000000 INFO @ Sat, 11 Dec 2021 06:26:09: 3000000 INFO @ Sat, 11 Dec 2021 06:26:11: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 06:26:11: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 06:26:11: #1 total tags in treatment: 888364 INFO @ Sat, 11 Dec 2021 06:26:11: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:26:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:26:11: #1 tags after filtering in treatment: 625710 INFO @ Sat, 11 Dec 2021 06:26:11: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 11 Dec 2021 06:26:11: #1 finished! INFO @ Sat, 11 Dec 2021 06:26:11: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:26:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:26:11: #2 number of paired peaks: 3084 INFO @ Sat, 11 Dec 2021 06:26:11: start model_add_line... INFO @ Sat, 11 Dec 2021 06:26:11: start X-correlation... INFO @ Sat, 11 Dec 2021 06:26:11: end of X-cor INFO @ Sat, 11 Dec 2021 06:26:11: #2 finished! INFO @ Sat, 11 Dec 2021 06:26:11: #2 predicted fragment length is 169 bps INFO @ Sat, 11 Dec 2021 06:26:11: #2 alternative fragment length(s) may be 169 bps INFO @ Sat, 11 Dec 2021 06:26:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.10_model.r WARNING @ Sat, 11 Dec 2021 06:26:11: #2 Since the d (169) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:26:11: #2 You may need to consider one of the other alternative d(s): 169 WARNING @ Sat, 11 Dec 2021 06:26:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:26:11: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:26:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:26:13: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:26:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.10_peaks.xls INFO @ Sat, 11 Dec 2021 06:26:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:26:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.10_summits.bed INFO @ Sat, 11 Dec 2021 06:26:14: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (681 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:26:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:26:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:26:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:26:25: 1000000 INFO @ Sat, 11 Dec 2021 06:26:32: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:26:39: 3000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:26:41: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 06:26:41: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 06:26:41: #1 total tags in treatment: 888364 INFO @ Sat, 11 Dec 2021 06:26:41: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:26:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:26:41: #1 tags after filtering in treatment: 625710 INFO @ Sat, 11 Dec 2021 06:26:41: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 11 Dec 2021 06:26:41: #1 finished! INFO @ Sat, 11 Dec 2021 06:26:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:26:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:26:41: #2 number of paired peaks: 3084 INFO @ Sat, 11 Dec 2021 06:26:41: start model_add_line... INFO @ Sat, 11 Dec 2021 06:26:41: start X-correlation... INFO @ Sat, 11 Dec 2021 06:26:41: end of X-cor INFO @ Sat, 11 Dec 2021 06:26:41: #2 finished! INFO @ Sat, 11 Dec 2021 06:26:41: #2 predicted fragment length is 169 bps INFO @ Sat, 11 Dec 2021 06:26:41: #2 alternative fragment length(s) may be 169 bps INFO @ Sat, 11 Dec 2021 06:26:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.20_model.r WARNING @ Sat, 11 Dec 2021 06:26:41: #2 Since the d (169) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:26:41: #2 You may need to consider one of the other alternative d(s): 169 WARNING @ Sat, 11 Dec 2021 06:26:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:26:41: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:26:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:26:43: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:26:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.20_peaks.xls INFO @ Sat, 11 Dec 2021 06:26:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:26:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556391/SRX8556391.20_summits.bed INFO @ Sat, 11 Dec 2021 06:26:44: Done! pass1 - making usageList (9 chroms): 0 millis pass2 - checking and writing primary data (453 records, 4 fields): 2 millis CompletedMACS2peakCalling