Job ID = 14170074 SRX = SRX8556365 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 31718618 spots for SRR12024921/SRR12024921.sra Written 31718618 spots for SRR12024921/SRR12024921.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170843 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:46:51 31718618 reads; of these: 31718618 (100.00%) were paired; of these: 8124128 (25.61%) aligned concordantly 0 times 16699905 (52.65%) aligned concordantly exactly 1 time 6894585 (21.74%) aligned concordantly >1 times ---- 8124128 pairs aligned concordantly 0 times; of these: 3492536 (42.99%) aligned discordantly 1 time ---- 4631592 pairs aligned 0 times concordantly or discordantly; of these: 9263184 mates make up the pairs; of these: 5931632 (64.03%) aligned 0 times 773207 (8.35%) aligned exactly 1 time 2558345 (27.62%) aligned >1 times 90.65% overall alignment rate Time searching: 01:46:51 Overall time: 01:46:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3693229 / 26821128 = 0.1377 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:15:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:15:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:15:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:15:08: 1000000 INFO @ Sat, 11 Dec 2021 07:15:16: 2000000 INFO @ Sat, 11 Dec 2021 07:15:23: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:15:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:15:31: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:15:31: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:15:31: 4000000 INFO @ Sat, 11 Dec 2021 07:15:39: 1000000 INFO @ Sat, 11 Dec 2021 07:15:40: 5000000 INFO @ Sat, 11 Dec 2021 07:15:48: 2000000 INFO @ Sat, 11 Dec 2021 07:15:49: 6000000 INFO @ Sat, 11 Dec 2021 07:15:57: 3000000 INFO @ Sat, 11 Dec 2021 07:15:58: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:16:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:16:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:16:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:16:06: 4000000 INFO @ Sat, 11 Dec 2021 07:16:07: 8000000 INFO @ Sat, 11 Dec 2021 07:16:10: 1000000 INFO @ Sat, 11 Dec 2021 07:16:15: 5000000 INFO @ Sat, 11 Dec 2021 07:16:16: 9000000 INFO @ Sat, 11 Dec 2021 07:16:19: 2000000 INFO @ Sat, 11 Dec 2021 07:16:24: 6000000 INFO @ Sat, 11 Dec 2021 07:16:25: 10000000 INFO @ Sat, 11 Dec 2021 07:16:28: 3000000 INFO @ Sat, 11 Dec 2021 07:16:33: 7000000 INFO @ Sat, 11 Dec 2021 07:16:34: 11000000 INFO @ Sat, 11 Dec 2021 07:16:37: 4000000 INFO @ Sat, 11 Dec 2021 07:16:42: 8000000 INFO @ Sat, 11 Dec 2021 07:16:43: 12000000 INFO @ Sat, 11 Dec 2021 07:16:47: 5000000 INFO @ Sat, 11 Dec 2021 07:16:51: 9000000 INFO @ Sat, 11 Dec 2021 07:16:52: 13000000 INFO @ Sat, 11 Dec 2021 07:16:56: 6000000 INFO @ Sat, 11 Dec 2021 07:17:00: 10000000 INFO @ Sat, 11 Dec 2021 07:17:01: 14000000 INFO @ Sat, 11 Dec 2021 07:17:05: 7000000 INFO @ Sat, 11 Dec 2021 07:17:09: 11000000 INFO @ Sat, 11 Dec 2021 07:17:11: 15000000 INFO @ Sat, 11 Dec 2021 07:17:14: 8000000 INFO @ Sat, 11 Dec 2021 07:17:18: 12000000 INFO @ Sat, 11 Dec 2021 07:17:20: 16000000 INFO @ Sat, 11 Dec 2021 07:17:23: 9000000 INFO @ Sat, 11 Dec 2021 07:17:27: 13000000 INFO @ Sat, 11 Dec 2021 07:17:29: 17000000 INFO @ Sat, 11 Dec 2021 07:17:32: 10000000 INFO @ Sat, 11 Dec 2021 07:17:36: 14000000 INFO @ Sat, 11 Dec 2021 07:17:38: 18000000 INFO @ Sat, 11 Dec 2021 07:17:42: 11000000 INFO @ Sat, 11 Dec 2021 07:17:45: 15000000 INFO @ Sat, 11 Dec 2021 07:17:47: 19000000 INFO @ Sat, 11 Dec 2021 07:17:51: 12000000 INFO @ Sat, 11 Dec 2021 07:17:54: 16000000 INFO @ Sat, 11 Dec 2021 07:17:57: 20000000 INFO @ Sat, 11 Dec 2021 07:18:00: 13000000 INFO @ Sat, 11 Dec 2021 07:18:03: 17000000 INFO @ Sat, 11 Dec 2021 07:18:06: 21000000 INFO @ Sat, 11 Dec 2021 07:18:09: 14000000 INFO @ Sat, 11 Dec 2021 07:18:12: 18000000 INFO @ Sat, 11 Dec 2021 07:18:15: 22000000 INFO @ Sat, 11 Dec 2021 07:18:18: 15000000 INFO @ Sat, 11 Dec 2021 07:18:21: 19000000 INFO @ Sat, 11 Dec 2021 07:18:24: 23000000 INFO @ Sat, 11 Dec 2021 07:18:27: 16000000 INFO @ Sat, 11 Dec 2021 07:18:30: 20000000 INFO @ Sat, 11 Dec 2021 07:18:33: 24000000 INFO @ Sat, 11 Dec 2021 07:18:36: 17000000 INFO @ Sat, 11 Dec 2021 07:18:39: 21000000 INFO @ Sat, 11 Dec 2021 07:18:42: 25000000 INFO @ Sat, 11 Dec 2021 07:18:46: 18000000 INFO @ Sat, 11 Dec 2021 07:18:48: 22000000 INFO @ Sat, 11 Dec 2021 07:18:51: 26000000 INFO @ Sat, 11 Dec 2021 07:18:55: 19000000 INFO @ Sat, 11 Dec 2021 07:18:57: 23000000 INFO @ Sat, 11 Dec 2021 07:19:00: 27000000 INFO @ Sat, 11 Dec 2021 07:19:04: 20000000 INFO @ Sat, 11 Dec 2021 07:19:06: 24000000 INFO @ Sat, 11 Dec 2021 07:19:09: 28000000 INFO @ Sat, 11 Dec 2021 07:19:13: 21000000 INFO @ Sat, 11 Dec 2021 07:19:15: 25000000 INFO @ Sat, 11 Dec 2021 07:19:18: 29000000 INFO @ Sat, 11 Dec 2021 07:19:22: 22000000 INFO @ Sat, 11 Dec 2021 07:19:23: 26000000 INFO @ Sat, 11 Dec 2021 07:19:27: 30000000 INFO @ Sat, 11 Dec 2021 07:19:31: 23000000 INFO @ Sat, 11 Dec 2021 07:19:32: 27000000 INFO @ Sat, 11 Dec 2021 07:19:37: 31000000 INFO @ Sat, 11 Dec 2021 07:19:40: 24000000 INFO @ Sat, 11 Dec 2021 07:19:41: 28000000 INFO @ Sat, 11 Dec 2021 07:19:46: 32000000 INFO @ Sat, 11 Dec 2021 07:19:49: 25000000 INFO @ Sat, 11 Dec 2021 07:19:50: 29000000 INFO @ Sat, 11 Dec 2021 07:19:55: 33000000 INFO @ Sat, 11 Dec 2021 07:19:58: 26000000 INFO @ Sat, 11 Dec 2021 07:19:59: 30000000 INFO @ Sat, 11 Dec 2021 07:20:04: 34000000 INFO @ Sat, 11 Dec 2021 07:20:07: 27000000 INFO @ Sat, 11 Dec 2021 07:20:08: 31000000 INFO @ Sat, 11 Dec 2021 07:20:13: 35000000 INFO @ Sat, 11 Dec 2021 07:20:16: 28000000 INFO @ Sat, 11 Dec 2021 07:20:17: 32000000 INFO @ Sat, 11 Dec 2021 07:20:22: 36000000 INFO @ Sat, 11 Dec 2021 07:20:25: 29000000 INFO @ Sat, 11 Dec 2021 07:20:26: 33000000 INFO @ Sat, 11 Dec 2021 07:20:31: 37000000 INFO @ Sat, 11 Dec 2021 07:20:34: 30000000 INFO @ Sat, 11 Dec 2021 07:20:35: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:20:40: 38000000 INFO @ Sat, 11 Dec 2021 07:20:43: 31000000 INFO @ Sat, 11 Dec 2021 07:20:44: 35000000 INFO @ Sat, 11 Dec 2021 07:20:50: 39000000 INFO @ Sat, 11 Dec 2021 07:20:53: 32000000 INFO @ Sat, 11 Dec 2021 07:20:53: 36000000 INFO @ Sat, 11 Dec 2021 07:20:59: 40000000 INFO @ Sat, 11 Dec 2021 07:21:02: 33000000 INFO @ Sat, 11 Dec 2021 07:21:02: 37000000 INFO @ Sat, 11 Dec 2021 07:21:08: 41000000 INFO @ Sat, 11 Dec 2021 07:21:11: 34000000 INFO @ Sat, 11 Dec 2021 07:21:11: 38000000 INFO @ Sat, 11 Dec 2021 07:21:17: 42000000 INFO @ Sat, 11 Dec 2021 07:21:20: 35000000 INFO @ Sat, 11 Dec 2021 07:21:20: 39000000 INFO @ Sat, 11 Dec 2021 07:21:27: 43000000 INFO @ Sat, 11 Dec 2021 07:21:29: 36000000 INFO @ Sat, 11 Dec 2021 07:21:30: 40000000 INFO @ Sat, 11 Dec 2021 07:21:36: 44000000 INFO @ Sat, 11 Dec 2021 07:21:38: 37000000 INFO @ Sat, 11 Dec 2021 07:21:39: 41000000 INFO @ Sat, 11 Dec 2021 07:21:45: 45000000 INFO @ Sat, 11 Dec 2021 07:21:48: 38000000 INFO @ Sat, 11 Dec 2021 07:21:48: 42000000 INFO @ Sat, 11 Dec 2021 07:21:54: 46000000 INFO @ Sat, 11 Dec 2021 07:21:57: 39000000 INFO @ Sat, 11 Dec 2021 07:21:57: 43000000 INFO @ Sat, 11 Dec 2021 07:22:03: 47000000 INFO @ Sat, 11 Dec 2021 07:22:06: 40000000 INFO @ Sat, 11 Dec 2021 07:22:06: 44000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 07:22:12: 48000000 INFO @ Sat, 11 Dec 2021 07:22:15: 41000000 INFO @ Sat, 11 Dec 2021 07:22:15: 45000000 INFO @ Sat, 11 Dec 2021 07:22:21: 49000000 INFO @ Sat, 11 Dec 2021 07:22:24: 42000000 INFO @ Sat, 11 Dec 2021 07:22:24: 46000000 INFO @ Sat, 11 Dec 2021 07:22:30: 50000000 INFO @ Sat, 11 Dec 2021 07:22:31: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 07:22:31: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 07:22:31: #1 total tags in treatment: 20251514 INFO @ Sat, 11 Dec 2021 07:22:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:22:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:22:32: #1 tags after filtering in treatment: 18990045 INFO @ Sat, 11 Dec 2021 07:22:32: #1 Redundant rate of treatment: 0.06 INFO @ Sat, 11 Dec 2021 07:22:32: #1 finished! INFO @ Sat, 11 Dec 2021 07:22:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:22:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:22:33: #2 number of paired peaks: 89 WARNING @ Sat, 11 Dec 2021 07:22:33: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:22:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:22:33: 47000000 INFO @ Sat, 11 Dec 2021 07:22:33: 43000000 INFO @ Sat, 11 Dec 2021 07:22:42: 48000000 INFO @ Sat, 11 Dec 2021 07:22:42: 44000000 INFO @ Sat, 11 Dec 2021 07:22:51: 49000000 INFO @ Sat, 11 Dec 2021 07:22:52: 45000000 INFO @ Sat, 11 Dec 2021 07:23:00: 50000000 INFO @ Sat, 11 Dec 2021 07:23:01: 46000000 INFO @ Sat, 11 Dec 2021 07:23:01: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 07:23:01: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 07:23:01: #1 total tags in treatment: 20251514 INFO @ Sat, 11 Dec 2021 07:23:01: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:23:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:23:01: #1 tags after filtering in treatment: 18990045 INFO @ Sat, 11 Dec 2021 07:23:01: #1 Redundant rate of treatment: 0.06 INFO @ Sat, 11 Dec 2021 07:23:01: #1 finished! INFO @ Sat, 11 Dec 2021 07:23:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:23:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:23:03: #2 number of paired peaks: 89 WARNING @ Sat, 11 Dec 2021 07:23:03: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:23:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:23:09: 47000000 INFO @ Sat, 11 Dec 2021 07:23:17: 48000000 INFO @ Sat, 11 Dec 2021 07:23:25: 49000000 INFO @ Sat, 11 Dec 2021 07:23:33: 50000000 INFO @ Sat, 11 Dec 2021 07:23:34: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 07:23:34: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 07:23:34: #1 total tags in treatment: 20251514 INFO @ Sat, 11 Dec 2021 07:23:34: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:23:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:23:34: #1 tags after filtering in treatment: 18990045 INFO @ Sat, 11 Dec 2021 07:23:34: #1 Redundant rate of treatment: 0.06 INFO @ Sat, 11 Dec 2021 07:23:34: #1 finished! INFO @ Sat, 11 Dec 2021 07:23:34: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:23:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:23:35: #2 number of paired peaks: 89 WARNING @ Sat, 11 Dec 2021 07:23:35: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:23:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556365/SRX8556365.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling