Job ID = 14170052 SRX = SRX8556362 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 37604366 spots for SRR12024918/SRR12024918.sra Written 37604366 spots for SRR12024918/SRR12024918.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171082 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 02:52:36 37604366 reads; of these: 37604366 (100.00%) were paired; of these: 13176289 (35.04%) aligned concordantly 0 times 17047826 (45.33%) aligned concordantly exactly 1 time 7380251 (19.63%) aligned concordantly >1 times ---- 13176289 pairs aligned concordantly 0 times; of these: 4870306 (36.96%) aligned discordantly 1 time ---- 8305983 pairs aligned 0 times concordantly or discordantly; of these: 16611966 mates make up the pairs; of these: 11990241 (72.18%) aligned 0 times 925062 (5.57%) aligned exactly 1 time 3696663 (22.25%) aligned >1 times 84.06% overall alignment rate Time searching: 02:52:37 Overall time: 02:52:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4966290 / 28964351 = 0.1715 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:32:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:32:11: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:32:11: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:32:22: 1000000 INFO @ Sat, 11 Dec 2021 08:32:34: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:32:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:32:40: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:32:40: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:32:46: 3000000 INFO @ Sat, 11 Dec 2021 08:32:49: 1000000 INFO @ Sat, 11 Dec 2021 08:32:58: 4000000 INFO @ Sat, 11 Dec 2021 08:32:58: 2000000 INFO @ Sat, 11 Dec 2021 08:33:07: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:33:10: 5000000 INFO @ Sat, 11 Dec 2021 08:33:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:33:10: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:33:10: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:33:15: 4000000 INFO @ Sat, 11 Dec 2021 08:33:23: 6000000 INFO @ Sat, 11 Dec 2021 08:33:23: 1000000 INFO @ Sat, 11 Dec 2021 08:33:24: 5000000 INFO @ Sat, 11 Dec 2021 08:33:34: 6000000 INFO @ Sat, 11 Dec 2021 08:33:35: 2000000 INFO @ Sat, 11 Dec 2021 08:33:35: 7000000 INFO @ Sat, 11 Dec 2021 08:33:43: 7000000 INFO @ Sat, 11 Dec 2021 08:33:49: 3000000 INFO @ Sat, 11 Dec 2021 08:33:49: 8000000 INFO @ Sat, 11 Dec 2021 08:33:52: 8000000 INFO @ Sat, 11 Dec 2021 08:34:01: 9000000 INFO @ Sat, 11 Dec 2021 08:34:02: 9000000 INFO @ Sat, 11 Dec 2021 08:34:02: 4000000 INFO @ Sat, 11 Dec 2021 08:34:10: 10000000 INFO @ Sat, 11 Dec 2021 08:34:14: 5000000 INFO @ Sat, 11 Dec 2021 08:34:15: 10000000 INFO @ Sat, 11 Dec 2021 08:34:19: 11000000 INFO @ Sat, 11 Dec 2021 08:34:28: 6000000 INFO @ Sat, 11 Dec 2021 08:34:28: 12000000 INFO @ Sat, 11 Dec 2021 08:34:28: 11000000 INFO @ Sat, 11 Dec 2021 08:34:37: 13000000 INFO @ Sat, 11 Dec 2021 08:34:41: 7000000 INFO @ Sat, 11 Dec 2021 08:34:41: 12000000 INFO @ Sat, 11 Dec 2021 08:34:46: 14000000 INFO @ Sat, 11 Dec 2021 08:34:54: 8000000 INFO @ Sat, 11 Dec 2021 08:34:54: 13000000 INFO @ Sat, 11 Dec 2021 08:34:55: 15000000 INFO @ Sat, 11 Dec 2021 08:35:04: 16000000 INFO @ Sat, 11 Dec 2021 08:35:07: 9000000 INFO @ Sat, 11 Dec 2021 08:35:08: 14000000 INFO @ Sat, 11 Dec 2021 08:35:13: 17000000 INFO @ Sat, 11 Dec 2021 08:35:20: 10000000 INFO @ Sat, 11 Dec 2021 08:35:21: 15000000 INFO @ Sat, 11 Dec 2021 08:35:21: 18000000 INFO @ Sat, 11 Dec 2021 08:35:30: 19000000 INFO @ Sat, 11 Dec 2021 08:35:33: 11000000 INFO @ Sat, 11 Dec 2021 08:35:34: 16000000 INFO @ Sat, 11 Dec 2021 08:35:39: 20000000 INFO @ Sat, 11 Dec 2021 08:35:46: 12000000 INFO @ Sat, 11 Dec 2021 08:35:46: 17000000 INFO @ Sat, 11 Dec 2021 08:35:48: 21000000 INFO @ Sat, 11 Dec 2021 08:35:59: 13000000 INFO @ Sat, 11 Dec 2021 08:36:00: 22000000 INFO @ Sat, 11 Dec 2021 08:36:00: 18000000 INFO @ Sat, 11 Dec 2021 08:36:10: 23000000 INFO @ Sat, 11 Dec 2021 08:36:11: 14000000 INFO @ Sat, 11 Dec 2021 08:36:12: 19000000 INFO @ Sat, 11 Dec 2021 08:36:19: 24000000 INFO @ Sat, 11 Dec 2021 08:36:25: 15000000 INFO @ Sat, 11 Dec 2021 08:36:26: 20000000 INFO @ Sat, 11 Dec 2021 08:36:28: 25000000 INFO @ Sat, 11 Dec 2021 08:36:37: 26000000 INFO @ Sat, 11 Dec 2021 08:36:37: 16000000 INFO @ Sat, 11 Dec 2021 08:36:39: 21000000 INFO @ Sat, 11 Dec 2021 08:36:45: 27000000 INFO @ Sat, 11 Dec 2021 08:36:50: 17000000 INFO @ Sat, 11 Dec 2021 08:36:51: 22000000 INFO @ Sat, 11 Dec 2021 08:36:54: 28000000 INFO @ Sat, 11 Dec 2021 08:37:03: 29000000 INFO @ Sat, 11 Dec 2021 08:37:03: 18000000 INFO @ Sat, 11 Dec 2021 08:37:04: 23000000 INFO @ Sat, 11 Dec 2021 08:37:12: 30000000 INFO @ Sat, 11 Dec 2021 08:37:16: 19000000 INFO @ Sat, 11 Dec 2021 08:37:17: 24000000 INFO @ Sat, 11 Dec 2021 08:37:21: 31000000 INFO @ Sat, 11 Dec 2021 08:37:29: 20000000 INFO @ Sat, 11 Dec 2021 08:37:30: 25000000 INFO @ Sat, 11 Dec 2021 08:37:32: 32000000 INFO @ Sat, 11 Dec 2021 08:37:40: 33000000 INFO @ Sat, 11 Dec 2021 08:37:43: 21000000 INFO @ Sat, 11 Dec 2021 08:37:43: 26000000 INFO @ Sat, 11 Dec 2021 08:37:49: 34000000 INFO @ Sat, 11 Dec 2021 08:37:55: 22000000 INFO @ Sat, 11 Dec 2021 08:37:56: 27000000 INFO @ Sat, 11 Dec 2021 08:37:58: 35000000 INFO @ Sat, 11 Dec 2021 08:38:07: 36000000 INFO @ Sat, 11 Dec 2021 08:38:08: 23000000 INFO @ Sat, 11 Dec 2021 08:38:08: 28000000 INFO @ Sat, 11 Dec 2021 08:38:16: 37000000 INFO @ Sat, 11 Dec 2021 08:38:20: 24000000 INFO @ Sat, 11 Dec 2021 08:38:21: 29000000 INFO @ Sat, 11 Dec 2021 08:38:25: 38000000 INFO @ Sat, 11 Dec 2021 08:38:33: 25000000 INFO @ Sat, 11 Dec 2021 08:38:34: 39000000 INFO @ Sat, 11 Dec 2021 08:38:34: 30000000 INFO @ Sat, 11 Dec 2021 08:38:43: 40000000 INFO @ Sat, 11 Dec 2021 08:38:46: 26000000 INFO @ Sat, 11 Dec 2021 08:38:47: 31000000 INFO @ Sat, 11 Dec 2021 08:38:52: 41000000 INFO @ Sat, 11 Dec 2021 08:38:59: 27000000 INFO @ Sat, 11 Dec 2021 08:39:00: 32000000 INFO @ Sat, 11 Dec 2021 08:39:01: 42000000 INFO @ Sat, 11 Dec 2021 08:39:10: 43000000 INFO @ Sat, 11 Dec 2021 08:39:12: 28000000 INFO @ Sat, 11 Dec 2021 08:39:13: 33000000 INFO @ Sat, 11 Dec 2021 08:39:19: 44000000 INFO @ Sat, 11 Dec 2021 08:39:25: 29000000 INFO @ Sat, 11 Dec 2021 08:39:26: 34000000 INFO @ Sat, 11 Dec 2021 08:39:28: 45000000 INFO @ Sat, 11 Dec 2021 08:39:37: 46000000 INFO @ Sat, 11 Dec 2021 08:39:38: 30000000 INFO @ Sat, 11 Dec 2021 08:39:39: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:39:46: 47000000 INFO @ Sat, 11 Dec 2021 08:39:51: 31000000 INFO @ Sat, 11 Dec 2021 08:39:52: 36000000 INFO @ Sat, 11 Dec 2021 08:39:55: 48000000 INFO @ Sat, 11 Dec 2021 08:40:03: 32000000 INFO @ Sat, 11 Dec 2021 08:40:04: 49000000 INFO @ Sat, 11 Dec 2021 08:40:05: 37000000 INFO @ Sat, 11 Dec 2021 08:40:13: 50000000 INFO @ Sat, 11 Dec 2021 08:40:15: 33000000 INFO @ Sat, 11 Dec 2021 08:40:17: 38000000 INFO @ Sat, 11 Dec 2021 08:40:22: 51000000 INFO @ Sat, 11 Dec 2021 08:40:28: 34000000 INFO @ Sat, 11 Dec 2021 08:40:29: 39000000 INFO @ Sat, 11 Dec 2021 08:40:32: 52000000 INFO @ Sat, 11 Dec 2021 08:40:40: 35000000 INFO @ Sat, 11 Dec 2021 08:40:41: 53000000 INFO @ Sat, 11 Dec 2021 08:40:42: 40000000 INFO @ Sat, 11 Dec 2021 08:40:44: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 08:40:44: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 08:40:44: #1 total tags in treatment: 20092995 INFO @ Sat, 11 Dec 2021 08:40:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:40:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:40:44: #1 tags after filtering in treatment: 18536940 INFO @ Sat, 11 Dec 2021 08:40:44: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 11 Dec 2021 08:40:44: #1 finished! INFO @ Sat, 11 Dec 2021 08:40:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:40:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:40:45: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 08:40:45: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:40:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:40:52: 36000000 INFO @ Sat, 11 Dec 2021 08:40:54: 41000000 INFO @ Sat, 11 Dec 2021 08:41:04: 37000000 INFO @ Sat, 11 Dec 2021 08:41:07: 42000000 INFO @ Sat, 11 Dec 2021 08:41:15: 38000000 INFO @ Sat, 11 Dec 2021 08:41:19: 43000000 INFO @ Sat, 11 Dec 2021 08:41:27: 39000000 INFO @ Sat, 11 Dec 2021 08:41:31: 44000000 INFO @ Sat, 11 Dec 2021 08:41:39: 40000000 INFO @ Sat, 11 Dec 2021 08:41:43: 45000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:41:52: 41000000 INFO @ Sat, 11 Dec 2021 08:41:55: 46000000 INFO @ Sat, 11 Dec 2021 08:42:04: 42000000 INFO @ Sat, 11 Dec 2021 08:42:08: 47000000 INFO @ Sat, 11 Dec 2021 08:42:17: 43000000 INFO @ Sat, 11 Dec 2021 08:42:20: 48000000 INFO @ Sat, 11 Dec 2021 08:42:28: 44000000 INFO @ Sat, 11 Dec 2021 08:42:32: 49000000 INFO @ Sat, 11 Dec 2021 08:42:40: 45000000 INFO @ Sat, 11 Dec 2021 08:42:44: 50000000 INFO @ Sat, 11 Dec 2021 08:42:52: 46000000 INFO @ Sat, 11 Dec 2021 08:42:56: 51000000 INFO @ Sat, 11 Dec 2021 08:43:04: 47000000 INFO @ Sat, 11 Dec 2021 08:43:08: 52000000 INFO @ Sat, 11 Dec 2021 08:43:17: 48000000 INFO @ Sat, 11 Dec 2021 08:43:21: 53000000 INFO @ Sat, 11 Dec 2021 08:43:25: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 08:43:25: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 08:43:25: #1 total tags in treatment: 20092995 INFO @ Sat, 11 Dec 2021 08:43:25: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:43:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:43:25: #1 tags after filtering in treatment: 18536940 INFO @ Sat, 11 Dec 2021 08:43:25: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 11 Dec 2021 08:43:25: #1 finished! INFO @ Sat, 11 Dec 2021 08:43:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:43:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:43:27: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 08:43:27: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:43:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:43:29: 49000000 INFO @ Sat, 11 Dec 2021 08:43:40: 50000000 INFO @ Sat, 11 Dec 2021 08:43:51: 51000000 INFO @ Sat, 11 Dec 2021 08:44:02: 52000000 INFO @ Sat, 11 Dec 2021 08:44:13: 53000000 INFO @ Sat, 11 Dec 2021 08:44:16: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 08:44:16: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 08:44:16: #1 total tags in treatment: 20092995 INFO @ Sat, 11 Dec 2021 08:44:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:44:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:44:17: #1 tags after filtering in treatment: 18536940 INFO @ Sat, 11 Dec 2021 08:44:17: #1 Redundant rate of treatment: 0.08 INFO @ Sat, 11 Dec 2021 08:44:17: #1 finished! INFO @ Sat, 11 Dec 2021 08:44:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:44:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:44:18: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 08:44:18: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:44:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8556362/SRX8556362.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling