Job ID = 14170095 SRX = SRX8556251 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 195026193 spots for SRR13595047/SRR13595047.sra Written 195026193 spots for SRR13595047/SRR13595047.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171512 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:51:49 195026193 reads; of these: 195026193 (100.00%) were paired; of these: 161930189 (83.03%) aligned concordantly 0 times 8570627 (4.39%) aligned concordantly exactly 1 time 24525377 (12.58%) aligned concordantly >1 times ---- 161930189 pairs aligned concordantly 0 times; of these: 2711759 (1.67%) aligned discordantly 1 time ---- 159218430 pairs aligned 0 times concordantly or discordantly; of these: 318436860 mates make up the pairs; of these: 294558337 (92.50%) aligned 0 times 1434081 (0.45%) aligned exactly 1 time 22444442 (7.05%) aligned >1 times 24.48% overall alignment rate Time searching: 03:51:50 Overall time: 03:51:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 72 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 23050529 / 35475907 = 0.6498 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:58:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:58:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:58:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:59:05: 1000000 INFO @ Sat, 11 Dec 2021 09:59:17: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:59:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:59:23: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:59:23: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:59:29: 3000000 INFO @ Sat, 11 Dec 2021 09:59:36: 1000000 INFO @ Sat, 11 Dec 2021 09:59:43: 4000000 INFO @ Sat, 11 Dec 2021 09:59:50: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:59:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:59:53: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:59:53: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:59:56: 5000000 INFO @ Sat, 11 Dec 2021 10:00:05: 3000000 INFO @ Sat, 11 Dec 2021 10:00:06: 1000000 INFO @ Sat, 11 Dec 2021 10:00:10: 6000000 INFO @ Sat, 11 Dec 2021 10:00:19: 4000000 INFO @ Sat, 11 Dec 2021 10:00:19: 2000000 INFO @ Sat, 11 Dec 2021 10:00:24: 7000000 INFO @ Sat, 11 Dec 2021 10:00:33: 3000000 INFO @ Sat, 11 Dec 2021 10:00:33: 5000000 INFO @ Sat, 11 Dec 2021 10:00:37: 8000000 INFO @ Sat, 11 Dec 2021 10:00:47: 4000000 INFO @ Sat, 11 Dec 2021 10:00:47: 6000000 INFO @ Sat, 11 Dec 2021 10:00:51: 9000000 INFO @ Sat, 11 Dec 2021 10:01:00: 5000000 INFO @ Sat, 11 Dec 2021 10:01:01: 7000000 INFO @ Sat, 11 Dec 2021 10:01:05: 10000000 INFO @ Sat, 11 Dec 2021 10:01:14: 6000000 INFO @ Sat, 11 Dec 2021 10:01:16: 8000000 INFO @ Sat, 11 Dec 2021 10:01:19: 11000000 INFO @ Sat, 11 Dec 2021 10:01:26: 7000000 INFO @ Sat, 11 Dec 2021 10:01:29: 9000000 INFO @ Sat, 11 Dec 2021 10:01:33: 12000000 INFO @ Sat, 11 Dec 2021 10:01:40: 8000000 INFO @ Sat, 11 Dec 2021 10:01:43: 10000000 INFO @ Sat, 11 Dec 2021 10:01:46: 13000000 INFO @ Sat, 11 Dec 2021 10:01:54: 9000000 INFO @ Sat, 11 Dec 2021 10:01:57: 11000000 INFO @ Sat, 11 Dec 2021 10:02:00: 14000000 INFO @ Sat, 11 Dec 2021 10:02:07: 10000000 INFO @ Sat, 11 Dec 2021 10:02:11: 12000000 INFO @ Sat, 11 Dec 2021 10:02:14: 15000000 INFO @ Sat, 11 Dec 2021 10:02:21: 11000000 INFO @ Sat, 11 Dec 2021 10:02:24: 13000000 INFO @ Sat, 11 Dec 2021 10:02:27: 16000000 INFO @ Sat, 11 Dec 2021 10:02:34: 12000000 INFO @ Sat, 11 Dec 2021 10:02:38: 14000000 INFO @ Sat, 11 Dec 2021 10:02:40: 17000000 INFO @ Sat, 11 Dec 2021 10:02:48: 13000000 INFO @ Sat, 11 Dec 2021 10:02:51: 15000000 INFO @ Sat, 11 Dec 2021 10:02:54: 18000000 INFO @ Sat, 11 Dec 2021 10:03:02: 14000000 INFO @ Sat, 11 Dec 2021 10:03:04: 16000000 INFO @ Sat, 11 Dec 2021 10:03:08: 19000000 INFO @ Sat, 11 Dec 2021 10:03:16: 15000000 INFO @ Sat, 11 Dec 2021 10:03:17: 17000000 INFO @ Sat, 11 Dec 2021 10:03:22: 20000000 INFO @ Sat, 11 Dec 2021 10:03:30: 18000000 INFO @ Sat, 11 Dec 2021 10:03:30: 16000000 INFO @ Sat, 11 Dec 2021 10:03:36: 21000000 INFO @ Sat, 11 Dec 2021 10:03:43: 19000000 INFO @ Sat, 11 Dec 2021 10:03:44: 17000000 INFO @ Sat, 11 Dec 2021 10:03:49: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 10:03:56: 20000000 INFO @ Sat, 11 Dec 2021 10:03:57: 18000000 INFO @ Sat, 11 Dec 2021 10:04:03: 23000000 INFO @ Sat, 11 Dec 2021 10:04:08: 21000000 INFO @ Sat, 11 Dec 2021 10:04:11: 19000000 INFO @ Sat, 11 Dec 2021 10:04:17: 24000000 INFO @ Sat, 11 Dec 2021 10:04:21: 22000000 INFO @ Sat, 11 Dec 2021 10:04:25: 20000000 INFO @ Sat, 11 Dec 2021 10:04:30: 25000000 INFO @ Sat, 11 Dec 2021 10:04:34: 23000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 10:04:38: 21000000 INFO @ Sat, 11 Dec 2021 10:04:43: 26000000 INFO @ Sat, 11 Dec 2021 10:04:47: 24000000 INFO @ Sat, 11 Dec 2021 10:04:51: 22000000 INFO @ Sat, 11 Dec 2021 10:04:57: 27000000 INFO @ Sat, 11 Dec 2021 10:05:00: 25000000 INFO @ Sat, 11 Dec 2021 10:05:04: 23000000 INFO @ Sat, 11 Dec 2021 10:05:10: 28000000 INFO @ Sat, 11 Dec 2021 10:05:13: 26000000 INFO @ Sat, 11 Dec 2021 10:05:17: 24000000 INFO @ Sat, 11 Dec 2021 10:05:23: 29000000 INFO @ Sat, 11 Dec 2021 10:05:26: 27000000 INFO @ Sat, 11 Dec 2021 10:05:30: 25000000 INFO @ Sat, 11 Dec 2021 10:05:36: 30000000 INFO @ Sat, 11 Dec 2021 10:05:39: 28000000 INFO @ Sat, 11 Dec 2021 10:05:43: 26000000 INFO @ Sat, 11 Dec 2021 10:05:50: 31000000 INFO @ Sat, 11 Dec 2021 10:05:53: 29000000 INFO @ Sat, 11 Dec 2021 10:05:56: 27000000 INFO @ Sat, 11 Dec 2021 10:06:03: 32000000 INFO @ Sat, 11 Dec 2021 10:06:06: 30000000 INFO @ Sat, 11 Dec 2021 10:06:09: 28000000 INFO @ Sat, 11 Dec 2021 10:06:16: 33000000 INFO @ Sat, 11 Dec 2021 10:06:19: 31000000 INFO @ Sat, 11 Dec 2021 10:06:22: 29000000 INFO @ Sat, 11 Dec 2021 10:06:29: 34000000 INFO @ Sat, 11 Dec 2021 10:06:33: 32000000 INFO @ Sat, 11 Dec 2021 10:06:35: 30000000 INFO @ Sat, 11 Dec 2021 10:06:42: 35000000 INFO @ Sat, 11 Dec 2021 10:06:46: 33000000 INFO @ Sat, 11 Dec 2021 10:06:49: 31000000 INFO @ Sat, 11 Dec 2021 10:06:55: 36000000 INFO @ Sat, 11 Dec 2021 10:06:59: 34000000 INFO @ Sat, 11 Dec 2021 10:07:02: 32000000 INFO @ Sat, 11 Dec 2021 10:07:08: 37000000 INFO @ Sat, 11 Dec 2021 10:07:12: 35000000 INFO @ Sat, 11 Dec 2021 10:07:16: 33000000 INFO @ Sat, 11 Dec 2021 10:07:21: 38000000 INFO @ Sat, 11 Dec 2021 10:07:25: 36000000 INFO @ Sat, 11 Dec 2021 10:07:30: 34000000 INFO @ Sat, 11 Dec 2021 10:07:34: 39000000 INFO @ Sat, 11 Dec 2021 10:07:38: 37000000 INFO @ Sat, 11 Dec 2021 10:07:43: 35000000 INFO @ Sat, 11 Dec 2021 10:07:47: 40000000 INFO @ Sat, 11 Dec 2021 10:07:50: 38000000 INFO @ Sat, 11 Dec 2021 10:07:57: 36000000 INFO @ Sat, 11 Dec 2021 10:08:00: 41000000 INFO @ Sat, 11 Dec 2021 10:08:03: 39000000 INFO @ Sat, 11 Dec 2021 10:08:10: 37000000 INFO @ Sat, 11 Dec 2021 10:08:13: 42000000 INFO @ Sat, 11 Dec 2021 10:08:16: 40000000 INFO @ Sat, 11 Dec 2021 10:08:24: 38000000 INFO @ Sat, 11 Dec 2021 10:08:27: 43000000 INFO @ Sat, 11 Dec 2021 10:08:28: 41000000 INFO @ Sat, 11 Dec 2021 10:08:37: 39000000 INFO @ Sat, 11 Dec 2021 10:08:40: 44000000 INFO @ Sat, 11 Dec 2021 10:08:42: 42000000 INFO @ Sat, 11 Dec 2021 10:08:51: 40000000 INFO @ Sat, 11 Dec 2021 10:08:53: 45000000 INFO @ Sat, 11 Dec 2021 10:08:55: 43000000 INFO @ Sat, 11 Dec 2021 10:09:04: 41000000 INFO @ Sat, 11 Dec 2021 10:09:07: 46000000 INFO @ Sat, 11 Dec 2021 10:09:08: 44000000 INFO @ Sat, 11 Dec 2021 10:09:18: 42000000 INFO @ Sat, 11 Dec 2021 10:09:21: 47000000 INFO @ Sat, 11 Dec 2021 10:09:21: 45000000 INFO @ Sat, 11 Dec 2021 10:09:32: 43000000 INFO @ Sat, 11 Dec 2021 10:09:34: 46000000 INFO @ Sat, 11 Dec 2021 10:09:35: 48000000 INFO @ Sat, 11 Dec 2021 10:09:46: 44000000 INFO @ Sat, 11 Dec 2021 10:09:48: 47000000 INFO @ Sat, 11 Dec 2021 10:09:48: 49000000 INFO @ Sat, 11 Dec 2021 10:09:53: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 10:09:53: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 10:09:53: #1 total tags in treatment: 10972740 INFO @ Sat, 11 Dec 2021 10:09:53: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:09:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:09:53: #1 tags after filtering in treatment: 7890908 INFO @ Sat, 11 Dec 2021 10:09:53: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 11 Dec 2021 10:09:53: #1 finished! INFO @ Sat, 11 Dec 2021 10:09:53: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:09:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:09:54: #2 number of paired peaks: 753 WARNING @ Sat, 11 Dec 2021 10:09:54: Fewer paired peaks (753) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 753 pairs to build model! INFO @ Sat, 11 Dec 2021 10:09:54: start model_add_line... INFO @ Sat, 11 Dec 2021 10:09:54: start X-correlation... INFO @ Sat, 11 Dec 2021 10:09:54: end of X-cor INFO @ Sat, 11 Dec 2021 10:09:54: #2 finished! INFO @ Sat, 11 Dec 2021 10:09:54: #2 predicted fragment length is 195 bps INFO @ Sat, 11 Dec 2021 10:09:54: #2 alternative fragment length(s) may be 195 bps INFO @ Sat, 11 Dec 2021 10:09:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.05_model.r WARNING @ Sat, 11 Dec 2021 10:09:54: #2 Since the d (195) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 10:09:54: #2 You may need to consider one of the other alternative d(s): 195 WARNING @ Sat, 11 Dec 2021 10:09:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 10:09:54: #3 Call peaks... INFO @ Sat, 11 Dec 2021 10:09:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 10:09:59: 45000000 INFO @ Sat, 11 Dec 2021 10:10:01: 48000000 INFO @ Sat, 11 Dec 2021 10:10:10: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 10:10:14: 46000000 INFO @ Sat, 11 Dec 2021 10:10:14: 49000000 INFO @ Sat, 11 Dec 2021 10:10:18: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.05_peaks.xls INFO @ Sat, 11 Dec 2021 10:10:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 10:10:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.05_summits.bed INFO @ Sat, 11 Dec 2021 10:10:18: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3903 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:10:19: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 10:10:19: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 10:10:19: #1 total tags in treatment: 10972740 INFO @ Sat, 11 Dec 2021 10:10:19: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:10:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:10:19: #1 tags after filtering in treatment: 7890908 INFO @ Sat, 11 Dec 2021 10:10:19: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 11 Dec 2021 10:10:19: #1 finished! INFO @ Sat, 11 Dec 2021 10:10:19: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:10:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:10:19: #2 number of paired peaks: 753 WARNING @ Sat, 11 Dec 2021 10:10:19: Fewer paired peaks (753) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 753 pairs to build model! INFO @ Sat, 11 Dec 2021 10:10:19: start model_add_line... INFO @ Sat, 11 Dec 2021 10:10:20: start X-correlation... INFO @ Sat, 11 Dec 2021 10:10:20: end of X-cor INFO @ Sat, 11 Dec 2021 10:10:20: #2 finished! INFO @ Sat, 11 Dec 2021 10:10:20: #2 predicted fragment length is 195 bps INFO @ Sat, 11 Dec 2021 10:10:20: #2 alternative fragment length(s) may be 195 bps INFO @ Sat, 11 Dec 2021 10:10:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.10_model.r WARNING @ Sat, 11 Dec 2021 10:10:20: #2 Since the d (195) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 10:10:20: #2 You may need to consider one of the other alternative d(s): 195 WARNING @ Sat, 11 Dec 2021 10:10:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 10:10:20: #3 Call peaks... INFO @ Sat, 11 Dec 2021 10:10:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 10:10:27: 47000000 INFO @ Sat, 11 Dec 2021 10:10:36: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 10:10:40: 48000000 INFO @ Sat, 11 Dec 2021 10:10:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.10_peaks.xls INFO @ Sat, 11 Dec 2021 10:10:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 10:10:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.10_summits.bed INFO @ Sat, 11 Dec 2021 10:10:44: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1951 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 10:10:52: 49000000 INFO @ Sat, 11 Dec 2021 10:10:57: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 10:10:57: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 10:10:57: #1 total tags in treatment: 10972740 INFO @ Sat, 11 Dec 2021 10:10:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 10:10:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 10:10:57: #1 tags after filtering in treatment: 7890908 INFO @ Sat, 11 Dec 2021 10:10:57: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 11 Dec 2021 10:10:57: #1 finished! INFO @ Sat, 11 Dec 2021 10:10:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 10:10:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 10:10:58: #2 number of paired peaks: 753 WARNING @ Sat, 11 Dec 2021 10:10:58: Fewer paired peaks (753) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 753 pairs to build model! INFO @ Sat, 11 Dec 2021 10:10:58: start model_add_line... INFO @ Sat, 11 Dec 2021 10:10:58: start X-correlation... INFO @ Sat, 11 Dec 2021 10:10:58: end of X-cor INFO @ Sat, 11 Dec 2021 10:10:58: #2 finished! INFO @ Sat, 11 Dec 2021 10:10:58: #2 predicted fragment length is 195 bps INFO @ Sat, 11 Dec 2021 10:10:58: #2 alternative fragment length(s) may be 195 bps INFO @ Sat, 11 Dec 2021 10:10:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.20_model.r WARNING @ Sat, 11 Dec 2021 10:10:58: #2 Since the d (195) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 10:10:58: #2 You may need to consider one of the other alternative d(s): 195 WARNING @ Sat, 11 Dec 2021 10:10:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 10:10:58: #3 Call peaks... INFO @ Sat, 11 Dec 2021 10:10:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 10:11:15: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 10:11:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.20_peaks.xls INFO @ Sat, 11 Dec 2021 10:11:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 10:11:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556251/SRX8556251.20_summits.bed INFO @ Sat, 11 Dec 2021 10:11:23: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (930 records, 4 fields): 3 millis CompletedMACS2peakCalling