Job ID = 14170094 SRX = SRX8556250 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 180466668 spots for SRR13595046/SRR13595046.sra Written 180466668 spots for SRR13595046/SRR13595046.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171800 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 05:44:01 180466668 reads; of these: 180466668 (100.00%) were paired; of these: 138422164 (76.70%) aligned concordantly 0 times 16685021 (9.25%) aligned concordantly exactly 1 time 25359483 (14.05%) aligned concordantly >1 times ---- 138422164 pairs aligned concordantly 0 times; of these: 5566310 (4.02%) aligned discordantly 1 time ---- 132855854 pairs aligned 0 times concordantly or discordantly; of these: 265711708 mates make up the pairs; of these: 245136178 (92.26%) aligned 0 times 2366241 (0.89%) aligned exactly 1 time 18209289 (6.85%) aligned >1 times 32.08% overall alignment rate Time searching: 05:44:01 Overall time: 05:44:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 84 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 30981616 / 47314181 = 0.6548 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:16:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:16:37: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:16:37: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:16:46: 1000000 INFO @ Sat, 11 Dec 2021 12:16:55: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:17:05: 3000000 INFO @ Sat, 11 Dec 2021 12:17:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:17:06: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:17:06: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:17:14: 4000000 INFO @ Sat, 11 Dec 2021 12:17:16: 1000000 INFO @ Sat, 11 Dec 2021 12:17:24: 5000000 INFO @ Sat, 11 Dec 2021 12:17:26: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 12:17:34: 6000000 INFO @ Sat, 11 Dec 2021 12:17:36: 3000000 INFO @ Sat, 11 Dec 2021 12:17:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 12:17:36: #1 read tag files... INFO @ Sat, 11 Dec 2021 12:17:36: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 12:17:45: 7000000 INFO @ Sat, 11 Dec 2021 12:17:45: 4000000 INFO @ Sat, 11 Dec 2021 12:17:47: 1000000 INFO @ Sat, 11 Dec 2021 12:17:55: 5000000 INFO @ Sat, 11 Dec 2021 12:17:56: 8000000 INFO @ Sat, 11 Dec 2021 12:17:58: 2000000 INFO @ Sat, 11 Dec 2021 12:18:05: 6000000 INFO @ Sat, 11 Dec 2021 12:18:07: 9000000 INFO @ Sat, 11 Dec 2021 12:18:10: 3000000 INFO @ Sat, 11 Dec 2021 12:18:15: 7000000 INFO @ Sat, 11 Dec 2021 12:18:18: 10000000 INFO @ Sat, 11 Dec 2021 12:18:22: 4000000 INFO @ Sat, 11 Dec 2021 12:18:25: 8000000 INFO @ Sat, 11 Dec 2021 12:18:29: 11000000 INFO @ Sat, 11 Dec 2021 12:18:33: 5000000 INFO @ Sat, 11 Dec 2021 12:18:34: 9000000 INFO @ Sat, 11 Dec 2021 12:18:41: 12000000 INFO @ Sat, 11 Dec 2021 12:18:44: 6000000 INFO @ Sat, 11 Dec 2021 12:18:46: 10000000 INFO @ Sat, 11 Dec 2021 12:18:52: 13000000 INFO @ Sat, 11 Dec 2021 12:18:55: 7000000 INFO @ Sat, 11 Dec 2021 12:18:56: 11000000 INFO @ Sat, 11 Dec 2021 12:19:03: 14000000 INFO @ Sat, 11 Dec 2021 12:19:06: 12000000 INFO @ Sat, 11 Dec 2021 12:19:06: 8000000 INFO @ Sat, 11 Dec 2021 12:19:14: 15000000 INFO @ Sat, 11 Dec 2021 12:19:16: 13000000 INFO @ Sat, 11 Dec 2021 12:19:17: 9000000 INFO @ Sat, 11 Dec 2021 12:19:25: 16000000 INFO @ Sat, 11 Dec 2021 12:19:27: 14000000 INFO @ Sat, 11 Dec 2021 12:19:29: 10000000 INFO @ Sat, 11 Dec 2021 12:19:37: 17000000 INFO @ Sat, 11 Dec 2021 12:19:38: 15000000 INFO @ Sat, 11 Dec 2021 12:19:40: 11000000 INFO @ Sat, 11 Dec 2021 12:19:48: 18000000 INFO @ Sat, 11 Dec 2021 12:19:48: 16000000 INFO @ Sat, 11 Dec 2021 12:19:51: 12000000 INFO @ Sat, 11 Dec 2021 12:19:59: 19000000 INFO @ Sat, 11 Dec 2021 12:20:00: 17000000 INFO @ Sat, 11 Dec 2021 12:20:02: 13000000 INFO @ Sat, 11 Dec 2021 12:20:10: 18000000 INFO @ Sat, 11 Dec 2021 12:20:10: 20000000 INFO @ Sat, 11 Dec 2021 12:20:14: 14000000 INFO @ Sat, 11 Dec 2021 12:20:22: 21000000 INFO @ Sat, 11 Dec 2021 12:20:22: 19000000 INFO @ Sat, 11 Dec 2021 12:20:25: 15000000 INFO @ Sat, 11 Dec 2021 12:20:32: 20000000 INFO @ Sat, 11 Dec 2021 12:20:33: 22000000 INFO @ Sat, 11 Dec 2021 12:20:36: 16000000 INFO @ Sat, 11 Dec 2021 12:20:42: 21000000 INFO @ Sat, 11 Dec 2021 12:20:43: 23000000 INFO @ Sat, 11 Dec 2021 12:20:48: 17000000 INFO @ Sat, 11 Dec 2021 12:20:52: 22000000 INFO @ Sat, 11 Dec 2021 12:20:54: 24000000 INFO @ Sat, 11 Dec 2021 12:20:59: 18000000 INFO @ Sat, 11 Dec 2021 12:21:01: 23000000 INFO @ Sat, 11 Dec 2021 12:21:04: 25000000 INFO @ Sat, 11 Dec 2021 12:21:10: 24000000 INFO @ Sat, 11 Dec 2021 12:21:11: 19000000 INFO @ Sat, 11 Dec 2021 12:21:14: 26000000 INFO @ Sat, 11 Dec 2021 12:21:21: 25000000 INFO @ Sat, 11 Dec 2021 12:21:22: 20000000 INFO @ Sat, 11 Dec 2021 12:21:25: 27000000 INFO @ Sat, 11 Dec 2021 12:21:30: 26000000 INFO @ Sat, 11 Dec 2021 12:21:34: 21000000 INFO @ Sat, 11 Dec 2021 12:21:35: 28000000 INFO @ Sat, 11 Dec 2021 12:21:39: 27000000 INFO @ Sat, 11 Dec 2021 12:21:45: 29000000 INFO @ Sat, 11 Dec 2021 12:21:45: 22000000 INFO @ Sat, 11 Dec 2021 12:21:48: 28000000 INFO @ Sat, 11 Dec 2021 12:21:56: 30000000 INFO @ Sat, 11 Dec 2021 12:21:56: 23000000 INFO @ Sat, 11 Dec 2021 12:21:57: 29000000 INFO @ Sat, 11 Dec 2021 12:22:06: 30000000 INFO @ Sat, 11 Dec 2021 12:22:06: 31000000 INFO @ Sat, 11 Dec 2021 12:22:07: 24000000 INFO @ Sat, 11 Dec 2021 12:22:15: 31000000 INFO @ Sat, 11 Dec 2021 12:22:17: 32000000 INFO @ Sat, 11 Dec 2021 12:22:17: 25000000 INFO @ Sat, 11 Dec 2021 12:22:24: 32000000 INFO @ Sat, 11 Dec 2021 12:22:27: 33000000 INFO @ Sat, 11 Dec 2021 12:22:28: 26000000 INFO @ Sat, 11 Dec 2021 12:22:33: 33000000 INFO @ Sat, 11 Dec 2021 12:22:38: 34000000 INFO @ Sat, 11 Dec 2021 12:22:39: 27000000 INFO @ Sat, 11 Dec 2021 12:22:42: 34000000 INFO @ Sat, 11 Dec 2021 12:22:49: 35000000 INFO @ Sat, 11 Dec 2021 12:22:50: 28000000 INFO @ Sat, 11 Dec 2021 12:22:51: 35000000 INFO @ Sat, 11 Dec 2021 12:23:00: 36000000 INFO @ Sat, 11 Dec 2021 12:23:01: 36000000 INFO @ Sat, 11 Dec 2021 12:23:02: 29000000 INFO @ Sat, 11 Dec 2021 12:23:10: 37000000 INFO @ Sat, 11 Dec 2021 12:23:10: 37000000 INFO @ Sat, 11 Dec 2021 12:23:14: 30000000 INFO @ Sat, 11 Dec 2021 12:23:20: 38000000 INFO @ Sat, 11 Dec 2021 12:23:20: 38000000 INFO @ Sat, 11 Dec 2021 12:23:26: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 12:23:30: 39000000 INFO @ Sat, 11 Dec 2021 12:23:30: 39000000 INFO @ Sat, 11 Dec 2021 12:23:36: 32000000 INFO @ Sat, 11 Dec 2021 12:23:39: 40000000 INFO @ Sat, 11 Dec 2021 12:23:41: 40000000 INFO @ Sat, 11 Dec 2021 12:23:47: 33000000 INFO @ Sat, 11 Dec 2021 12:23:49: 41000000 INFO @ Sat, 11 Dec 2021 12:23:51: 41000000 INFO @ Sat, 11 Dec 2021 12:23:57: 34000000 INFO @ Sat, 11 Dec 2021 12:23:58: 42000000 INFO @ Sat, 11 Dec 2021 12:24:01: 42000000 INFO @ Sat, 11 Dec 2021 12:24:08: 35000000 INFO @ Sat, 11 Dec 2021 12:24:08: 43000000 INFO @ Sat, 11 Dec 2021 12:24:12: 43000000 INFO @ Sat, 11 Dec 2021 12:24:18: 36000000 INFO @ Sat, 11 Dec 2021 12:24:18: 44000000 INFO @ Sat, 11 Dec 2021 12:24:23: 44000000 INFO @ Sat, 11 Dec 2021 12:24:28: 37000000 INFO @ Sat, 11 Dec 2021 12:24:28: 45000000 INFO @ Sat, 11 Dec 2021 12:24:35: 45000000 INFO @ Sat, 11 Dec 2021 12:24:38: 46000000 INFO @ Sat, 11 Dec 2021 12:24:38: 38000000 INFO @ Sat, 11 Dec 2021 12:24:46: 46000000 INFO @ Sat, 11 Dec 2021 12:24:48: 47000000 INFO @ Sat, 11 Dec 2021 12:24:48: 39000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 12:24:57: 47000000 INFO @ Sat, 11 Dec 2021 12:24:58: 40000000 INFO @ Sat, 11 Dec 2021 12:24:59: 48000000 INFO @ Sat, 11 Dec 2021 12:25:08: 41000000 INFO @ Sat, 11 Dec 2021 12:25:09: 48000000 INFO @ Sat, 11 Dec 2021 12:25:10: 49000000 INFO @ Sat, 11 Dec 2021 12:25:19: 42000000 INFO @ Sat, 11 Dec 2021 12:25:20: 50000000 INFO @ Sat, 11 Dec 2021 12:25:20: 49000000 INFO @ Sat, 11 Dec 2021 12:25:30: 43000000 INFO @ Sat, 11 Dec 2021 12:25:30: 51000000 INFO @ Sat, 11 Dec 2021 12:25:32: 50000000 INFO @ Sat, 11 Dec 2021 12:25:40: 52000000 INFO @ Sat, 11 Dec 2021 12:25:41: 44000000 INFO @ Sat, 11 Dec 2021 12:25:43: 51000000 INFO @ Sat, 11 Dec 2021 12:25:50: 53000000 INFO @ Sat, 11 Dec 2021 12:25:53: 45000000 INFO @ Sat, 11 Dec 2021 12:25:54: 52000000 INFO @ Sat, 11 Dec 2021 12:25:58: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 12:25:58: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 12:25:58: #1 total tags in treatment: 13756107 INFO @ Sat, 11 Dec 2021 12:25:58: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:25:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:25:58: #1 tags after filtering in treatment: 10280753 INFO @ Sat, 11 Dec 2021 12:25:58: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 12:25:58: #1 finished! INFO @ Sat, 11 Dec 2021 12:25:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:25:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:25:59: #2 number of paired peaks: 314 WARNING @ Sat, 11 Dec 2021 12:25:59: Fewer paired peaks (314) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 314 pairs to build model! INFO @ Sat, 11 Dec 2021 12:25:59: start model_add_line... INFO @ Sat, 11 Dec 2021 12:25:59: start X-correlation... INFO @ Sat, 11 Dec 2021 12:25:59: end of X-cor INFO @ Sat, 11 Dec 2021 12:25:59: #2 finished! INFO @ Sat, 11 Dec 2021 12:25:59: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 12:25:59: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 12:25:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.10_model.r WARNING @ Sat, 11 Dec 2021 12:25:59: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 12:25:59: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Sat, 11 Dec 2021 12:25:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 12:25:59: #3 Call peaks... INFO @ Sat, 11 Dec 2021 12:25:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 12:26:04: 46000000 INFO @ Sat, 11 Dec 2021 12:26:05: 53000000 INFO @ Sat, 11 Dec 2021 12:26:14: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 12:26:14: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 12:26:14: #1 total tags in treatment: 13756107 INFO @ Sat, 11 Dec 2021 12:26:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:26:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:26:14: #1 tags after filtering in treatment: 10280753 INFO @ Sat, 11 Dec 2021 12:26:14: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 12:26:14: #1 finished! INFO @ Sat, 11 Dec 2021 12:26:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:26:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:26:15: #2 number of paired peaks: 314 WARNING @ Sat, 11 Dec 2021 12:26:15: Fewer paired peaks (314) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 314 pairs to build model! INFO @ Sat, 11 Dec 2021 12:26:15: start model_add_line... INFO @ Sat, 11 Dec 2021 12:26:15: 47000000 INFO @ Sat, 11 Dec 2021 12:26:15: start X-correlation... INFO @ Sat, 11 Dec 2021 12:26:15: end of X-cor INFO @ Sat, 11 Dec 2021 12:26:15: #2 finished! INFO @ Sat, 11 Dec 2021 12:26:15: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 12:26:15: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 12:26:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.05_model.r WARNING @ Sat, 11 Dec 2021 12:26:15: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 12:26:15: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Sat, 11 Dec 2021 12:26:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 12:26:15: #3 Call peaks... INFO @ Sat, 11 Dec 2021 12:26:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 12:26:21: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 12:26:25: 48000000 INFO @ Sat, 11 Dec 2021 12:26:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.10_peaks.xls INFO @ Sat, 11 Dec 2021 12:26:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 12:26:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.10_summits.bed INFO @ Sat, 11 Dec 2021 12:26:33: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1874 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:26:36: 49000000 INFO @ Sat, 11 Dec 2021 12:26:36: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 12:26:46: 50000000 INFO @ Sat, 11 Dec 2021 12:26:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.05_peaks.xls INFO @ Sat, 11 Dec 2021 12:26:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 12:26:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.05_summits.bed INFO @ Sat, 11 Dec 2021 12:26:49: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4172 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 12:26:55: 51000000 INFO @ Sat, 11 Dec 2021 12:27:05: 52000000 INFO @ Sat, 11 Dec 2021 12:27:15: 53000000 INFO @ Sat, 11 Dec 2021 12:27:23: #1 tag size is determined as 150 bps INFO @ Sat, 11 Dec 2021 12:27:23: #1 tag size = 150 INFO @ Sat, 11 Dec 2021 12:27:23: #1 total tags in treatment: 13756107 INFO @ Sat, 11 Dec 2021 12:27:23: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 12:27:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 12:27:23: #1 tags after filtering in treatment: 10280753 INFO @ Sat, 11 Dec 2021 12:27:23: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 12:27:23: #1 finished! INFO @ Sat, 11 Dec 2021 12:27:23: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 12:27:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 12:27:24: #2 number of paired peaks: 314 WARNING @ Sat, 11 Dec 2021 12:27:24: Fewer paired peaks (314) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 314 pairs to build model! INFO @ Sat, 11 Dec 2021 12:27:24: start model_add_line... INFO @ Sat, 11 Dec 2021 12:27:24: start X-correlation... INFO @ Sat, 11 Dec 2021 12:27:24: end of X-cor INFO @ Sat, 11 Dec 2021 12:27:24: #2 finished! INFO @ Sat, 11 Dec 2021 12:27:24: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 12:27:24: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 12:27:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.20_model.r WARNING @ Sat, 11 Dec 2021 12:27:24: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 12:27:24: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Sat, 11 Dec 2021 12:27:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 12:27:24: #3 Call peaks... INFO @ Sat, 11 Dec 2021 12:27:24: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 12:27:45: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 12:27:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.20_peaks.xls INFO @ Sat, 11 Dec 2021 12:27:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 12:27:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8556250/SRX8556250.20_summits.bed INFO @ Sat, 11 Dec 2021 12:27:58: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (769 records, 4 fields): 6 millis CompletedMACS2peakCalling